35 results about "Kidney transplantation" patented technology

A novel and effective strategy for organ transplantation is provided. A therapeutic agent and method for preventing an acute rejection in a transplanted organ and improving prognosis are provided. A therapeutic agent for suppressing a rejection in organ transplantation, which comprises an NF-κB decoy compound, is provided. Representatively, the organ transplantation is kidney transplantation. The therapeutic agent further comprises an ultrasonic inspection contrast agent. A therapeutic agent for improving prognosis in organ transplantation is also provided. The therapeutic agent is administered into a donor organ. The transfection efficiency of the NF-κB decoy compound into the organ may be enhanced by ultrasound treatment.

A method of treating a kidney disease in a subject is disclosed. The method is effected by transplanting into the subject a graft of nephric tissue at a predetermined developmental stage thereby treating the kidney disease in the subject.

The invention discloses a method for detecting serum polypeptide for kidney transplantation rejection. The method comprises the following steps of: 1, classifying the previously collected serum samples according to the seriousness of the disease of a donor; 2, capturing the polypeptide from the serum in the serum sample groups by using MB-WCX respectively and concentrating the polypeptide on magnetic balls to form tie molecules; 3, eluting the tie molecules from the magnetic balls and performing MALDI-TOF mass spectrometric analysis; classifying the different peptide peaks measured from the samples of each group to establish a classifying and predicting model so as to acquire a difference peptide expression map; 4, combining the serum samples of a kidney transplantation rejection patient to be detected by using the MB-WCX, then performing the MALDI-TOF mass spectrometric analysis and comparing the analysis result with the map acquired in the step 3 so as to mark the peptide of the serum sample. The method contributes to understanding the pathogenesis for the kidney transplantation rejection well from the level of the proteomics and makes the early diagnosis of the kidney transplantation rejection possible.

Provided herein are methods, compositions, and kits for diagnosing acute rejection of renal transplants using the gene expression profile of sets of classifier genes. Such methods and compositions are independent of external confounders such as recipient age, transplant center, RNA source, assay, cause of end-stage renal disease, co-morbidities, immunosuppression usage, and the like.

The invention discloses a kidney transplantation specimen storage device. The storage device comprises a shell, wherein an inner box is arranged in the shell, an air delivery pipe is arranged in the inner box, an air delivery pipe base is fixedly connected to the lower end of the air delivery pipe, blades capable of being overturned and opened are arranged in the air delivery pipe base, a liftingframe is fixedly connected to the outer side of the air delivery pipe base, lifting racks are fixedly connected to the front side and the rear side of the two ends of the lifting frame, lifting mechanisms capable of controlling the lifting frame to ascend and descend are meshed on the outer side of the lifting racks, a specimen placement barrel is arranged at the lower end of the air delivery pipebase, a placement table capable of moving back and forth is arranged at the lower end of the specimen placement barrel, and a sealing door is hinged to the left side of the front end of the shell. According to the storage device, a test tube for storing a specimen can be placed in a specimen placement box, the specimen placement box can be taken out from the specimen placement barrel by pressingthe specimen placement box, operation is convenient and efficient, circulation of cold air can be controlled by controlling blades, operation and using of a user are facilitated, the fixation stability of the specimen placement barrel is enhanced by the arrangement of the sealing door, and the safety of the specimen is ensured.

The present invention relates to a hepatitis B vaccine, the main composition of said vaccine comprises gene engineering hepatitis B virus surface antigen, muramyl dipeptide (MDP) and aluminium adjuvant. Said hepatitis B vaccine can be used for immunity of adult, renal transplanted patient and patient with renal dialysis therapy and for preventing infection of hepatitis B virus, and said hepatitis B vaccine also can be used for immunotherapy of chronic hepatitis B patient, and the immunogenicity of said vaccine is superior to that of existent aluminium adjuvant hepatitis B vaccine.

The invention discloses a use of Klotho gene editing in hetero-kidney transplantation. Through Klotho gene editing, acute and chronic damage to a hetero-kidney is prevented, hetero-kidney normal state and functions are kept and survival time of the hetero-kidney in a receptor is prolonged.

The invention relates to a urine-derived stem cell preparation and a preparation method thereof, and an application of the preparation in preparation of medicines for resisting immune rejection reaction after organ transplantation. The method comprises the steps: selecting clean middle section urine of healthy volunteers; carrying out separated culture and in-vitro amplification by using a culturemedium, and detecting the in-vitro proliferation capacity; detecting the expression level of surface molecular markers by flow cytometry, and detecting the expression level of kidney-associated proteins by immunoblotting; take the third-sixth generations of urinary stem cells identified, meeting the requirements and with confluence of 80% in logarithmic growth phase; preparing the urine-derived stem cell preparation by further digestion, centrifugation and physiological saline resuspension. The urine-derived stem cell preparation has the advantages of wide source, low acquisition cost and high separation efficiency. The urine-derived stem cell preparation can remarkably prolong the survival time of rats after kidney transplantation, reduce the levels of urea nitrogen and creatinine and reduce the infiltration of T cells in kidney tissues; meanwhile, the beating time of a transplant in the mouse heart transplantation can be obviously prolonged.

The invention provides a method of a biomarker for identifying renal transplantation prognosis and a detection kit for identifying the renal transplantation prognosis. The biomarker is a ratio of freemannose and glucose obtained by pre-column 1-phenyl-5-methyl pyrazolone (PMP) derivatization high performance liquid chromatography in serum. The detection method is a pre-column PMP derivatization high performance liquid chromatography method. The technical scheme has the advantages that the pretreatment is simple, the analysis time is short, the instrument price is reasonable, the conventionaluse requirements are met, the operation steps are simple and easy to learn, the accuracy of a detection result is high, only blood collection is needed, the needed serum amount is extremely small, theblood collection amount is smaller than 1 mL, and the like. The obtained result shows that the analysis method can quickly quantify the free mannose and glucose in the serum of a renal transplantation prognosis patient, and has very important significance for researching a relation between the free mannose and glucose in the serum and the renal transplantation prognosis, and finding a novel clinical prognosis monitoring marker for the renal transplantation prognosis.

The invention belongs to the field of serum drug concentration monitoring after renal transplantation, and discloses a primer set, a kit and an evaluation method for evaluating tacrolimus metabolism.Specifically, genotypes of five SNP loci (CYP3A5 rs15524, CYP3A5 rs4646453, POR rs2286822, POR rs1135612 and POR rs1057868) on two important genes CYP3A5 and POR related to tacrolimus metabolism are detected to predict individual metabolic conditions of tacrolimus in a patient, so as to provide medication guidance for individualized design of an initial dosage of tacrolimus.

The invention belongs to the technical field of pharmaceutical dosage forms and nano medicine preparation, and more particularly relates to a micelle loaded with Tacrolimus or pharmaceutical salts thereof, a lyophilized preparation as well as preparation methods and applications thereof. In order to achieve the clinical application of the micelle provided by the invention, the drug loading, the encapsulation rate and the stability of the preparation need to be ensured. The micelle is prepared from biodegradable polymer materials and Tacrolimus or pharmaceutical salts thereof in parts by weight: 1 part of Tacrolimus or pharmaceutical salts thereof and 1.5-99 parts of degradable polymer materials, wherein the degradable polymer materials are diblock polymer methoxy polyethylene glycol-polycaprolactone (the molecular weight ratio of MPEG to PCL is 0.5-2), and triblock polymer polycaprolactone-polyethylene glycol-polycaprolactone (the molecular weight ratio of PCL to PEG is 0.5-3). The micelle using Tacrolimus or pharmaceutical salts thereof or the lyophilized preparation of the micelle can be used in medicines for anti-rejection in liver transplantation, kidney transplantation, lung transplantation, corneal transplantation and other organ transplantations.

The invention belongs to the field of medical instruments and relates to a fixing device, in particular to a limb fixing device for kidney transplantation operation, comprising a base, an adapter chute is rotatably connected to the base, and an arm supporting device is slidably connected to the adapter chute. Both sides of the base are provided with movable sliding grooves which are communicated with the adapter sliding grooves. The arm support device comprises a pair of guide rails slidably connected with the adapter chute, and a slider is slidably connected with the guide rails; One end of the slider far away from the adapter chute is connected with a sliding seat, an upper sliding seat is slidably connected with the sliding seat, a lower supporting plate is connected with the upper sliding seat, an upper supporting plate is arranged above the lower supporting plate, a pair of telescopic rods are arranged on the upper sliding seat, and the telescopic ends of the telescopic rods are rotatably connected with the upper supporting plate base fixedly connected with the upper supporting plate; a support plate is arranged above that base, and a buttock clamping device is arranged on thesupport plate. The invention effectively solves the problems that the arm of a patient is prone to sour anesthesia and the fixation effect of the patient is not good in the operation process.

Disclosed is a compound electrolytic fructose injection for fluid replacement treatment in diuresis period after kidney transplantation surgery, wherein levulose is used for substituting glucose, for making compound injection with correct concentration range, The volume of the transfusion bottle and the transfusion bag is increased, thus simplifying transfusion operational program, reducing working capacity of fluid replacement treatment, and overcoming the possible pollution and error caused by frequent replacement of transfusion bottle or bag.

The invention discloses a post-renal transplantation wound protector which comprises a bottom plate, a connecting mechanism, a relief hole, a protection sleeve, a radiation mechanism, a fixing mechanism, a control mechanism and an air exchange mechanism. By adopting the post-renal transplantation wound protector, a postoperative wound part of a patient after renal transplant is at a state of beingisolated from the outside, accidents that the wound part of the patient is extruded by an external article and harm can be done to the wound of the patient can be avoided, and in addition, red lightradiation treatment on t he wound of the patient can be implemented by using the device, so that healing of the wound of the patient can be promoted in an assistant manner; and therefore, by adoptingthe device disclosed by the invention, not only is a protection function on the wound of the patient achieved, but also the rehabilitation progress of the patient can be promoted.

Provided herein are methods, compositions, and kits for diagnosing acute rejection of renal transplants using the gene expression profile of sets of classifier genes. Such methods and compositions are independent of external confounders such as recipient age, transplant center, RNA source, assay, cause of end-stage renal disease, co-morbidities, immunosuppression usage, and the like.

The invention relates to a method for detecting harm degree of postoperative cytomegalovirus infection of a kidney transplantation patient. The invention includes the following steps: a, collecting blood; b, acquiring and analyzing CD19+B lymphocytes; c, preparing cytomegalovirus seeds and quantifying; d, culturing the CD19+B lymphocytes in vitro, and using HCMV to directly and indirectly stimulate the CD19+B lymphocytes; e, performing flow detection on expression of a BAFF receptor on the CD19+B lymphocytes; f, detecting apoptosis rate of the CD19+B lymphocytes; g, detecting IgG secretion capability of the CD19+B lymphocytes. By the method, reaction, to HCMV stimulation, of the B lymphocytes of the patient can be analyzed. The method has important value in evaluating chronic rejection which is indirect effect caused by HCMV infection of the kidney transplantation patient.

The invention discloses an extravascular stent for kidney transplantation, and belongs to the field of kidney transplantation surgeries. The extravascular stent comprises a telescopic part, and the telescopic part comprises two telescopic plates; the ends, close to each other, of the two telescopic plates are in threaded installation with a bidirectional threaded rod at the same time, and two support shields are arranged on the lower side of the telescopic part; the two supporting shields are both adjusted and controlled through a driven mechanism, the two driven mechanisms are jointly in transmission fit with a driving mechanism, each supporting shield comprises two arc-shaped pieces which are matched with each other, and the top end of each arc-shaped piece is rotatably installed on thebottom wall of the telescopic plate. The overall size of the stent can be conveniently adjusted, meanwhile, the two supporting shields are arranged and can be crossed with each other, so that the internal space of the supporting shields can be adjusted according to needs, renal artery or renal vein can be well limited and bound, and therefore the stent has a good operation effect and is suitable for popularization and application. And the complex actual operation requirements can be better met.

The invention discloses a distraction fixator for a kidney transplantation operation, and relates to the field of kidney transplantation operations. The distraction fixator for the kidney transplantation operation comprises a bed body, a supporting and positioning base, a lifting supporting mechanism and a distraction fixator structure, the bottom of the bed body is fixedly connected with two sets of supporting blocks, the two sets of supporting blocks are symmetrically distributed, and a reinforcing transverse plate is fixedly connected between the two sets of supporting blocks. When the distraction fixator for the kidney transplantation operation is used, the position can be adjusted according to the body position of a patient, then the rectangular frame is installed and fixed to the upper portion of the bottom supporting platform through the lifting supporting mechanism, and the position of the rectangular frame is adjusted according to the weight of the patient; and finally, the distraction arms and the distraction plates can be installed according to the incision condition of the patient, and the distance and the angle between the distraction arms and the distraction plates can be adjusted, so that the distraction effect is guaranteed.

The invention discloses a kidney preservation bag in kidney transplantation, which comprises a heat preservation box, a sealing cover, a moving mechanism, an exhaust mechanism, a containing bag, avoiding holes, a self-sealing zipper and a hanging belt. According to the kidney preservation bag in kidney transplantation, Firstly, the device can carry out isolated storage treatment on an ice bag anda kidney needing to be transplanted, the ice bag is subjected to heat preservation treatment in a closed environment, and the medical personnel discharge cold air of the ice bag to the kidney area byusing the moving mechanism of the device, so that the kidney is refrigerated and stored. Therefore, the device can prevent the ice bag from being in direct contact with the kidney, the kidney is protected, and the operation effect is ensured.

The invention discloses a model system for kidney transplantation rejection resistance type classification and a construction method of the model system, and solves the problem that various limitations exist in prediction and judgment of kidney transplantation rejection resistance at present. According to the technical scheme, the method is characterized by comprising a sample substrate, a sample data set, a Raman spectrometer, a classification model and actual sample data, wherein the Raman spectrometer is used for detecting samples to obtain spectral data; the classification model is established for classification prediction; the actual sample data is used for verifying classification results of the classification model; the method comprises the following steps: detecting a sample through a Raman spectrometer to obtain spectral data, training and testing an established classification model through the spectral data to obtain a convergent classification model, verifying the obtained classification model through actual sample data, and carrying out classification discrimination through the classification model. According to the model system for renal transplantation anti-rejection reaction type classification and the construction method thereof, the classification model can be efficiently, simply and conveniently obtained, and then classification judgment can be accurately carried out.

The present invention relates to a medicine for treating chronic renal failure. The chronic renal failure is the final result of various kidney diseases, but without effective western medicine in clinic yet, and dialysis therapy and renal transplantation price are expensive and not suitable for early and middle patients. The medicine whole formula of the present invention both activates and tonifies, consideration to right and evil, nourishing and releasing integrated, leading nourishing not stagnating evil, releasing not injuring right, can not only delay chronic renal failure course, but also improve or eliminate clinical symptoms, having good curative effect, lower cost characteristic. The present invention concretely is with radix astragali, poria, hedyotis diffusa, serissa foetida, pyrola calliantha, ligusticum chuanxiong, morinda officinalis how, centella asiatica, prepared rhubarb and etc. traditional Chinese medicine to prepare into compound preparation. The present invention also relates to the medicine preparation method. As pharmacological experiment shows the present invention can obviously decrease adenine causing chronic renal failure rat and nephrectomy causing chronic renal failure rat kidney function Crea and BUN value, the present invention can also obviously increase water load rat urinary volume.

The invention belongs to the technical field of pharmaceutical dosage forms and nano medicine preparation, and more particularly relates to a micelle loaded with Tacrolimus or pharmaceutical salts thereof, a lyophilized preparation as well as preparation methods and applications thereof. In order to achieve the clinical application of the micelle provided by the invention, the drug loading, the encapsulation rate and the stability of the preparation need to be ensured. The micelle is prepared from biodegradable polymer materials and Tacrolimus or pharmaceutical salts thereof in parts by weight: 1 part of Tacrolimus or pharmaceutical salts thereof and 1.5-99 parts of degradable polymer materials, wherein the degradable polymer materials are diblock polymer methoxy polyethylene glycol-polycaprolactone (the molecular weight ratio of MPEG to PCL is 0.5-2), and triblock polymer polycaprolactone-polyethylene glycol-polycaprolactone (the molecular weight ratio of PCL to PEG is 0.5-3). The micelle using Tacrolimus or pharmaceutical salts thereof or the lyophilized preparation of the micelle can be used in medicines for anti-rejection in liver transplantation, kidney transplantation, lung transplantation, corneal transplantation and other organ transplantations.

The invention discloses a method for detecting serum polypeptide for kidney transplantation rejection. The method comprises the following steps of: 1, classifying the previously collected serum samples according to the seriousness of the disease of a donor; 2, capturing the polypeptide from the serum in the serum sample groups by using MB-WCX respectively and concentrating the polypeptide on magnetic balls to form tie molecules; 3, eluting the tie molecules from the magnetic balls and performing MALDI-TOF mass spectrometric analysis; classifying the different peptide peaks measured from the samples of each group to establish a classifying and predicting model so as to acquire a difference peptide expression map; 4, combining the serum samples of a kidney transplantation rejection patient to be detected by using the MB-WCX, then performing the MALDI-TOF mass spectrometric analysis and comparing the analysis result with the map acquired in the step 3 so as to mark the peptide of the serum sample. The method contributes to understanding the pathogenesis for the kidney transplantation rejection well from the level of the proteomics and makes the early diagnosis of the kidney transplantation rejection possible.

The invention discloses a novel application of CX-5461 in preparation of a medicine for treating renal transplantation immunological rejection. The research shows that CX-5461 can obviously reduce the occurrence of renal transplantation immunological rejection, specifically, the fibrosis degree and immune infiltration degree of a renal graft are reduced, the creatinine and urea nitrogen levels in blood after a rat renal transplantation operation are reduced, the IL-17A cell factor level of the rat renal graft and the in-vivo proinflammatory IL-6 cell factor level are reduced, and the like. Compared with the prior art, the CX-5461 has the advantages that the CX-5461 can be used as a novel immunosuppressive agent for treating renal transplantation immunological rejection, especially acute rejection after renal transplantation, and can be used for preventing and treating renal transplantation immunological rejection, and the survival time of rats can be prolonged, and the long-term survival rate of rats after renal transplantation can be obviously prolonged. The CX-5461 can be used as a novel immunosuppressive agent for treating renal transplantation immunological rejection, especially acute rejection after renal transplantation.

Provided herein are methods, compositions, and kits for diagnosing acute rejection of renal transplants using the gene expression profile of sets of classifier genes. Such methods and compositions are independent of external confounders such as recipient age, transplant center, RNA source, assay, cause of end-stage renal disease, co-morbidities, immunosuppression usage, and the like.

The invention relates to the technical field of biology, in particular to applications of SNP (single nucleotide polymorphism) sites as well as primers, probes and a detection kit for the SNP sites, and particularly provides applications of 40 SNP sites in evaluation of transplant damage and rejection risk after renal transplantation. The 40 specific SNP sites of total free DNA of a receptor after transplantation are detected, and by means of combination with a specific calculation method, content of free DNA of a donor in free DNA of the receptor is obtained finally, that is, GcfDNA donor-receptor ratio is obtained; the degree of transplant rejection is judged rapidly and accurately according to the GcfDNA donor-receptor ratio, any intrusive detection is not needed, and one simple and effective auxiliary means is provided for clinical detection of the transplant damage and rejection risk after organ transplantation.

The invention relates to a construction method of a model for predicting the dose of a kidney transplantation drug by using an endogenous substance, and the method comprises the following steps: collecting a blood sample and a use effect of an individual after using an immunosuppressive agent; metabolic characteristics and clinical characteristics which are highly related to the use effect are screened according to the combination of the metabolic characteristics and clinical characteristics in the blood sample and the use effect, and then the screened metabolic characteristics and clinical characteristics are used to establish a drug dose prediction model of the immunosuppressor. The model constructed by the method provided by the invention can predict the dosage applicable to individuals, avoids potential adverse drug reaction, and is beneficial to individual drug administration.