Sphingomonaspaucimobilis of high-yield gellan gum and use therefor

A technology of gellan gum and monosporus bacteria, applied in the direction of bacteria, fermentation, etc., can solve the problems of long fermentation period, complex medium, low yield, etc., and achieve the effect of increasing fermentation yield, simple fermentation medium, and reducing costs

Inactive Publication Date: 2007-05-30
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] (1) In the above process 1, the fermentation medium for producing gellan gum by fermentation with glucose as a carbon source all contains complex salt solution, the medium is complicated, the production cost is high, the product quality is unstable, and the yield is low
[0010] (2) In the above-mentioned process 2, starch is used as the carbon source, and the bacterial strain has relatively high requirements on nitrogen source, the conversion rate of fermentation is low, and it is difficult to remove starch in the post-treatment process
[0011] (3) The fermentation period in the above process is long (50-72 hours), the rubber yield is low (1.0-1.6%), the conversion rate is low (45-50%), and the viscosity of the fermentation broth is 4,000-8,000cP, which is low The extraction rate of acyl gellan gum is less than 50%
Compared with xanthan gum, complex medium, long fermentation cycle, low yield and low conversion rate are the main obstacles to the large-scale industrialization of gellan gum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Screening, cultivation and identification of Sphingomonas paucimobilis XLJ CCTCC No.M206058

[0037]Soil and water samples were obtained from the sewage outlet of the refinery, soaked in basic inorganic salt medium (MAMD) overnight, and incubated at 30°C and 180 rpm for 48 hours with shaking. The soil samples were removed from the culture medium and then centrifuged to obtain the cells. The obtained cells were washed twice with normal saline, suspended in a potassium phosphate buffer solution with a concentration of 100 mM pH 7.0, and the concentration of the cells was adjusted to 2 g dry cells / liter. Add this bacterial solution to the above-mentioned basic inorganic salt medium test tube containing 2% glucose at an inoculum of 10% by volume, incubate at 30°C for 24 hours, and then transfer it to a fresh tube with an inoculum of 10% by volume The culture was continued for 2 more days in minimal mineral salt medium containing 2% glucose. Use the basic inorgan...

Embodiment 2

[0044] Embodiment 2: Production of microbial polysaccharide gellan gum by fermentation of Sphingomonas paucimobilis

[0045] (1) Strain selection: select Sphingomonas paucimobilis XLJ CCTCC No.M206058;

[0046] (2) Slant culture: inoculate the above-mentioned bacterial strains on a solid slant medium, and cultivate them for 20 hours at 30°C;

[0047] (3) First-level seed cultivation: the bacteria cultivated in step (2) were placed in 100mL seed liquid medium with an inoculation loop under sterile conditions, and shaken for 10 hours at 30°C to obtain first-class seed liquid;

[0048] (4) Expanded cultivation: with an inoculum size of 5% by volume, connect the primary seed solution to 500 mL seed liquid culture medium, and culture under shaking for 8 hours at 30° C. to obtain the secondary seed solution;

[0049] (5) Fermentation tank cultivation: with the inoculum size of 6% by volume ratio, connect the secondary seed solution in 50L liquid fermentation medium, under the cond...

Embodiment 3

[0056] Embodiment 3: Production of microbial polysaccharide gellan gum by fermentation of Sphingomonas paucimobilis

[0057] (1) Strain selection: select Sphingomonas paucimobilis XLJ CCTCC No.M206058;

[0058] (2) Slant culture: inoculate the above-mentioned bacterial strains on a solid slant medium, and cultivate them for 24 hours at 25°C;

[0059] (3) First-level seed cultivation: the thallus cultivated in step (2) was put into 50mL seed liquid culture medium with an inoculation loop under sterile conditions, and shaken for 12 hours at 25°C to obtain first-class seed liquid;

[0060] (4) Expanded cultivation: with an inoculum size of 8% by volume, connect the primary seed solution to 500 mL seed liquid culture medium, and culture under shaking for 10 hours at 25° C. to obtain the secondary seed solution;

[0061] (5) Fermentation tank cultivation: with the inoculum size of 6% by volume ratio, connect the secondary seed liquid in 10L liquid fermentation medium, under the c...

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PUM

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Abstract

The invention discloses a manufacturing method of Shingomonas paucimobilis XLJ with reserving number at M206058, which comprises the following steps: (1) selecting bacterial; (2) culturing through slope; (3) culturing one-grade seed; (4) enlarging culture; (5) culturing in the ferment tank; (6) collecting ferment product.

Description

technical field [0001] The present invention relates to a strain of Sphingomonas paucikineta and its application, in particular to a strain of Sphingomonas paucikineta with high yield of gellan gum and the use of Sphingomonas paucikineta to ferment and produce microbial polysaccharides The cold glue method. Background technique [0002] Gellan gum is a new type of microbial polysaccharide produced by Sphingomonas paucimobilis. It has excellent characteristics such as low dosage, high transparency, strong aroma release ability, acid resistance, high temperature resistance, enzyme resistance, and good compatibility. It can be applied It is a biological material with high profit and great development potential in many fields such as food, beverage, cosmetics, detergent, ceramics, oil exploration, chemical coating, etc. [0003] Gellan gum was first discovered in 1978, and in 1992 it was certified by the US FDA. The European Community also officially included it in the food sa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04
Inventor 许平王霞马翠卿
Owner SHANDONG UNIV
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