Bacillus amyloliquefaciens D2WM as well as preparation method and application of bacillus amyloliquefaciens D2WM

Abstract

The invention discloses a bacillus amyloliquefaciens D2WM as well as a preparation method and application of bacillus amyloliquefaciens D2WM. The bacillus amyloliquefaciens D2WM comprises the steps of A, bacillus separation and purification, namely collecting after inoculating a slope; B, preliminary screening of bacillus, namely sequentially selecting strains having antagonistic effects on Erwinia and camellia oleifera diseases by using a point connection method and a plate confrontation method; and C, secondary screening of bacillus, namely culturing the preliminarily-screened strains with the antagonistic effects in an LB liquid culture medium by using a cup-plate method, and screening the strains simultaneously having the antagonistic effects on main diseases of Erwinia and camellia oleifera through an antagonistic bacterium fermentation liquid and sterile filtrate. When being applied to preparation of drugs for treating or preventing soft rot of vegetables and camellia oleifera diseases, the bacillus amyloliquefaciens has a broad-spectrum antagonistic effect, high in growth speed, capable of efficiently antagonizing anthracnose of camellia oleifera, leaf spot diseases of camellia oleifera, black spot of camellia oleifera, canker of camellia oleifera and various diseases and remarkable in antibacterial effect, the culture time for general bacteria and fungi is greatly shortened, and the production cost is reduced.

Description

Technical field [0001] The invention relates to the field of plant protection, and more specifically to a method for preparing Bacillusamyloliquefaciens D2WM which can simultaneously antagonize vegetable soft rot and main diseases of camellia oleifera, and also relates to a preparation method of Bacillusamyloliquefaciens D2WM, and also relates to a starch solution Application of Bacillus Bacillusamyloliquefaciens D2WM in preparing medicine for treating or preventing vegetable soft rot and camellia disease. Background technique [0002] Vegetable soft rot is also known as water rotten and bumps. In addition to harming cruciferous vegetables (such as radish, Chinese cabbage, etc.), it can also harm vegetables such as potatoes, tomatoes, lettuce, cucumber, celery and onions, causing varying degrees of loss. In the early stage of the onset of vegetable soft rot, the diseased part is water-soaked and translucent, and then expanded to light gray-brown wet rot, the diseased tissue is s...

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Problems solved by technology

Sun Ming etc. have invented a kind of Bacillus subtilis BSn5 and antibacterial protein APn5 (application number: 200710053427.8) that have bacteriostatic activity to Erwinia carotovora soft rot, the bacteriostatic active substance of this Bacillus subtilis is protein APn5, can only Antagonism against Erwinia subspecies of carrot soft rot, its biocontrol range is relatively narrow; Huang Jing et al. disclosed that a kind of Bacillus amyloliquefaciens Lh-1 is effective in the prevention and treatment of cotton verticillium wilt, Chinese cabbage soft rot, bacterial wilt, and peach tree rot. disease, apple rot, etc. (application number: 201410735508.6), it is necessary to use Bacillus amyloliquefaciens and chitosan oligosaccharides to form a microecological bacterial agent, which is relatively effective only for the control of cotton verticillium wilt; Liu Junang et al. A Bacillus brevis Z26 that antagonizes camellia oleifera disease is disclosed, which can effectively inhibit the growth of pathogenic bacteria such as camellia oleifera anthracnose, root rot, and hemimania (application number: 201110027957.1). The rate reached 83.4%, and there is still room for further improvement; Zhou Guoying and others invented a compound microbial source pesticide and its application method (application number: 201310203845.6) for effectively preventing and controlling the disease of Camellia oleifera, and disclosed a kind of pesticide produced by the actinomycete Streptomyces coccidioides The microbial pesticide compounded by coccidioides subspecies, Bacillus brevis and other additives can prevent one or several major diseases of camellia oleifera. The preparation process of the compound microbial pesticide is relatively cumbersome and takes a long time; Related reports of Bacillus and Bacillus thuringiensis ML1 on the control of peanut fruit rot and konjac soft rot respectively (application number: 201310708367.4, application number: 201510015310.5)

However, there are few reports on the prevention and control of Bacillus amyloliquefaciens on vegetable soft rot and major diseases of Camellia oleifera

In addition, most of the literature reports are for the single control of a certain disease of camellia oleifera. At present, there is basically no single bacterial agent product that can prevent and treat more than two kinds of camellia oleifera diseases at the same time. Reports on two diseases

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