49 results about "Sphingomonas paucimobilis" patented technology

The invention discloses a sphingomonas paucimobilis strain and an application thereof. The sphingomonas paucimobilis strain is named as sphingomonas paucimobilis QHZJUJW, with the preservation number: CGMCC No.7783. The stain is endophyte derived and screened from seeds of wild microporous grass in Qinghai-Tibet Plateau, can be used for industrialized production of gellan gum and provides a new way for the industrialized production of the endophyte and gellan gum.

In the invention, a strain for efficient production of gellan gum is obtained by separation and screening of paddy root soil from Sha County, Sanming, Fujian province. The strain is Sphingomonas paucimobilis strain FJAT-5627. The invention also reveals a method for preparing gellan gum from the Sphingomonas paucimobilis strain FJAT-5627. The invention has the advantages that: a new Sphingomonas paucimobilis strain FJAT-5627 is provided and utilized to prepare gellan gum, and the prepared gellan gum has a yield meeting the industrialized production requirement, thereby increasing the sources of gellan gum production strains in line with the industrialized production requirements.

The invention relates to a strain for degrading phenol compounds and application of the strain. The strain is classified and named as Sphingomonas paucimobilis DL-10 and has been preserved in China Center for Type Culture Collection on March 3, 2014, with a preservation number of CCTCC NO: M 2014058. A 16S rDNA nucleotide sequence of the strain is as shown in SEQ ID NO: 1 in a sequence list. The strain DL-10 is an aerobic microorganism, wherein the optimal growth temperature is 30 DEG C, the optimal growth pH is 6.0, and the growth is obviously limited when the NaCl concentration is higher than 3%. By virtue of the strain DL-10, 100mg/L phenol can be completely degraded within 12h, and by taking the strain DL-10 as a unique carbon source for growth, the phenol compounds such as catechol, hydroquinone, o-methylphenol, m-methylphenol, 2,6-dimethylphenol, 3,5-dimethylphenol, 2,6-dimethyl hydroquinone can be completely degraded. The strain has important application value in the biological treatment of the phenol compounds in industrial wastewater.

A method of reducing the content of TSNA in tobacco leaves, comprising treating tobacco leaves with a microorganism which has the capability to reduce TSNA and which is selected from the group consisting of Sphingomonas paucimobilis and Pseudomonas fluorescens.

The present invention provides a method for preparing a moxifloxacin side chain through a biological method, particularly to a method for preparing a compound represented by a formula 2. The method comprises: catalyzing a compound represented by a formula 4 with transaminase to form a compound represented by formula 3, and carrying out spontaneous ring closure on the compound represented by the formula 3 to obtain the compound represented by the formula 2, wherein the transaminase is selected from the omega-transaminase in Arthrobacter, Aspergillus terreus, Vibrio fluvialis, Bacillus megaterium, Sphingomonas paucimobilis, Hyphomonasneptunium, and Chromobacterium violaceum, and in the formulas 2, 3 and 4, R is selected from benzyloxycarbonyl, benzyl and ethoxycarbonyl. The formulas 2, 3 and 4 are defined in the specification.

The invention discloses a Sphingomonas paucimobilis strain and application thereof and belongs to the technical field of bioengineering. The Sphingomonas paucimobilis strain is named Sphingomonas paucimobilis strain Y5, is collected in CCTCC (China Center for Type Culture Collection), which is located in Wuhan University, Wuhan, China, and has the collection number of CCTCC NO: M 2015523. Welan gum can be prepared from the train by fermenting under appropriate conditions through carbon sources, nitrogen sources, inorganic salts and water . The strain disclosed by the invention has relatively high sugar conversion ratio and welan gum yield and has a certain application value. The welan gum produced through fermenting the strain is high in quality and is resistant to acids and bases, a 1% aqueous solution viscosity of the welan gum reaches 3500mPa.S, and the viscosity is fundamentally unchanged at the temperature of 150 DEG C. During the fermentation of the strain, the sugar conversion ratio is relatively high and reaches 55% to 60%, and the yield is relatively high and reaches 25-32g/L (dry weight).

The invention provides the DNA sequence of a lycopene cyclase gene (crtY) in sphingomonas sp., a recombinant strain lacking lycopene cyclase and use thereof. The nucleotide sequence of the crtY gene is represented by SEQ ID NO.1. The DNA sequence of the lycopene cyclase gene (crtY) in sphingomonas sp., which is provided by the invention, lays a foundation for the genetic modification of a sphingomonas sp. carotenoid biological synthesis means. In addition, compared with a wild strain, the sphingomonas sp. recombinant strain lacking the lycopene cyclase gene, which is constructed by a gene knockout method, has the advantages that: the gellengum yield is basically unchanged; and lycopene can be produced in the production of gellengum by fermentation. And the strain can be used for further gene knockout or metabolic engineering modification.

The invention provides the DNA sequences of a beta-carotene hydroxylase gene (crtZ) and a 2,2'-betahydroxylase gene (crtG) in sphingomonas sp., recombinant strains lacking beta-carotene hydroxylase and 2,2'-betahydroxylase and the use of the two strains. The nucleotide sequence of the crtZ gene is represented by SEQ ID NO.1; and the nucleotide sequence of the crtG gene is represented by SEQ ID NO.2. The DNA sequences of the beta-carotene hydroxylase gene and the 2,2'-betahydroxylase gene in the sphingomonas sp., which are provided by the invention, lay a foundation for the genetic modification of a sphingomonas sp. carotenoid biological synthesis means. In addition, compared with wild strains, the recombinant strains of the sphingomonas sp., which are constructed by a gene knockout method, have the advantages that: gellengum yield is basically unchanged; and beta-carotene or zeaxanthin can be produced in the production of gellengum by fermentation. And the strains can be used in further gene knockout or metabolic engineering modification.

A solid microbial inoculum for remediating Cd in farmland soil, comprising: curtobacterium luteum, cupriavidus necator, sphingomonas parapaucimobilis, cellulosimicrobium cellulans, azotobacter sp., saccharomyces sp., streptomyces sp. and pseudomonas sp. Acetic acid solubility of Cd, Cu and Zn in soil that is remediated by the solid microbial inoculum disclosed by the invention can be decreased significantly.

The present invention belongs to the technical field of soil environments, and specifically relates to a microbial soil improvement agent for petroleum polluted soil. The microbial soil improvement agent comprises the following raw materials: Paxillus involutus Hypha, Hebeloma mesophaeusm, Acinetobacter calcoaceticus, Sphingomonas paucimobilis, pyrene degrading bacteria, Glomus etunicatum, Cephalosporium roseum, Aureobasidium pullulans, nitrilase, long-chain alkane degradation enzyme, integration membrane di iron alkane hydroxylase, phenylalanine deaminase, arginine dihydrolase, Rhodococcus erythropolis, pseudomonas aeruginosa, sodium oleate, thiamine hydrochloride, calcium pantothenate, oyster shell powder, vermiculite powder, paspalum wettsteinii hackel powder, kentucky eupatorium fortunei powder, miscanthus floridulus powder, medicago sativa powder, pig manure granules, goat manure granules, gamma-polyglutamic acid, malic acid, citric acid, and Nostocales powder. According to the present invention, various types of the microorganisms in the improvement agent synergistically coordinate, and co-act with the carrier raw materials; and with the application of the improvement agent to treat different types of crude oil polluted soil in the Shengli oil field, the total removal rate of the petroleum hydrocarbon in the polluted soil achieves 77.68-89.76%.

An object of the present invention is to provide a method for producing ethanol from polysaccharide alginate contained in a large amounts in brown algae, using the alginate assimilation capacity of the Sphingomonas sp. strain A1 and the strong ethanol production capacity of bacteria such as Zymomonas mobilis. Specifically, a method for producing ethanol using alginate as a raw material comprises causing genes encoding proteins and enzymes involved in Sphingomonas sp. strain A1-derived alginate assimilation and genes encoding enzymes involved in ethanol production to co-exist in a single microorganism and then culturing the microorganism in a medium containing alginate.

The invention belongs to the field of preparation of microbiological polysaccharide solution, and especially relates to a preparation method of a paste welan gum. The preparation method comprises the following steps: inoculating sphingomonas paucimobilis (CGMCC No.1561) to a sterile fermentation culture medium, which contains a carbon source, a nitrogen source, inorganic salts, and water; then carrying out extraction and solid-liquid separation, and finally manufacturing the paste welan gum. In the prior art, the welan gum powder cannot be directly applied to oil-based slurry in petroleum drilling wells and the operation safety of welan gum powder is bad. The provided paste welan gum solves the problems mentioned above. The preparation method is simple. The paste welan gum is suitable for industrial production, transportation and preservation. The stability of the paste welan gum is good. The paste welan gum can be rapidly and evenly mixed with oil-based slurry. Moreover, no dust is generated during the using process, the environment is protected, and the material consumption is reduced.

The invention provides a sphingomonas paucimobilis gene knockout mutant strain and a construction method thereof. The strain is collected in China Center for Type Culture Collection and has the collection number of CCTCC No. 2016610. A PHB deletion sphingomonas paucimobilis gene knockout mutant strain is constructed, and genes of acetoacetyl coenzyme A reductase in a PHB synthetic route of sphingomonas paucimobilis are knocked out by utilizing homologous recombination so as to obtain the gene deletion mutant strain, so that production of the PHB is inhibited, and the purity and transparency of colloid are improved; and meanwhile, the colloid productive rate and colloid production stability are maintained, so that the possibility of removing transparent high acyl gellan gum of the PHB in large-scale production is greatly improved.

The invention comprises a photoautotrophic organism, generally having simpler nutritional requirements than heterotrophic organisms, utilized as a chassis for the heterologous expression and function of enzymes, or derivatives of said enzymes, that show activity toward the degradation/detoxification of toxins known to be associated with and specific to harmful algal blooms. As an example, a cyanobacterial strain (Synechocystis sp. PCC 6803) modified to express Sphingomonas sp. USTB-05 MlrA enzyme functionality, showing the capability of degrading microcystins (results shown here) and nodularins, is presented. Under modelled natural conditions, results indicate that heterologous enzymatic activity against microcystin-LR is more stable over time when utilizing a photoautotrophic chassis in comparison to use of a heterotrophic bacterial strain. In addition, both the viability and cell density of the photoautotrophic host is maintained for a significantly longer period of time, compared to a heterotrophic host.

The invention discloses a pseudomonas aeruginosa and sphingomonas paucimobilis inactivating method based on a water supply network growth ring and relates to a method for inactivating two kinds of pathogenic bacteria, namely pseudomonas aeruginosa and sphingomonas paucimobilis in drinking water. The method aims to solve the problem that an existing method for inactivating pseudomonas aeruginosa and sphingomonas paucimobilis in drinking water is poor in effect. The method comprises the single step of adding alpha-FeOOH and H2O2 to a clean water reservoir of a city water supply plant. According to the method, the cost of H2O2 is low, alpha-FeOOH is cheap and easy to obtain, is a main constituent of the growth ring and can exist continuously in a city water supply network after being added once, only H2O2 needs to be added in the later period, the requirement for equipment is simple, energy consumption is low, and investment and operation cost are reduced. A Fenton-like body formed by alpha-FeOOH and H2O2 can inactivate pseudomonas aeruginosa and sphingomonas paucimobilis existing in filtered water remarkably.

The invention discloses a modified illite powder microbial agent as well as a preparation method and application thereof. The modified illite powder microbial agent is prepared from the following components in parts by weight: 75-85 parts of an adsorption carrier and 15-25 parts of a mixed strain fermentation product, wherein the adsorption carrier is prepared by loading illite powder with water-soluble chitosan or a derivative of the water-soluble chitosan and then modifying the illite powder through ethanol, the mixed strain fermentation product is prepared by carrying out slant culture andshake culture on original strains of achromobacter xylosoxidans, sphingomonas paucimobilis and bacillus megatherium respectively and sequentially under the sterile condition, then inoculating a liquidfermentation medium with the original strains jointly, carrying out mixed culture through a fermentation tank, and then carrying out centrifuging, concentrating and drying, and the viable count of the mixed strain fermentation product is 20-30 billion cfu/ml. According to the modified illite powder microbial agent as well as the preparation method and application thereof, a product is applied during soil preparation, heavy metal ions can be immobilized in situ, the biological effectiveness of the heavy metal cadmium in soil can be effectively reduced, and the absorption of crops to the heavymetal cadmium ions can be reduced.

The invention discloses a purification method of a cyclina sinensis finished product. The purification method comprises the following steps: washing an obtained cyclina sinensis finished product; removing sludge; putting into a temporary cultivation tank; during a cultivation period, adding a microalgae preparation into sand-filtered seawater, wherein the microalgae is a mixture of skeletonema, tetraselmis and dicrateria inornata which are mixed at a random ratio; after 5 hours, putting the cyclina sinensis finished product into another temporary cultivation tank; adding a microbial preparation into the sand-filtered seawater, wherein the microbial preparation contains agritol freeze-dried powder, bacillus subtilis freeze-dried powder, bacillus licheniformis freeze-dried powder, wild mushroom entity freeze-dried powder and diatomite; and culturing for 24-48 hours, renewing the water and further culturing for 4-8 hours to obtain a purified finished product. The purification method disclosed by the invention has the advantages of effectively removing heavy metals in the body of the cyclina sinensis within short time, having a quite good removal effect on escherichia coli, sphingomonas paucimobilis, vibrio and aeromonas salmonicida, and is simple to operate, short in purification time and extremely good in purification efficiency.

The invention provides gellan gum/curdlan compound microbial food gum and a preparation method thereof. The preparation method comprises the following steps: S1, inoculating a culture medium which takes glucose as a carbon source with sphingomonas paucimobilis and alcaligenes faecalis, and carrying out aerobic fermentation; S2, adjusting fermentation liquor to be alkaline after fermentation, performing water-bath heating until the temperature is 50-60 DEG C, preserving heat for 1-2 hours, and performing solid-liquid separation to obtain supernate rich in microbial food gum; and S3, adjusting the pH value of the supernate to be neutral, adding ethanol into the supernate, and performing standing for 1-12 hours, and performing solid-liquid separation to obtain the gellan gum/curdlan compound microbial food gum. Compared with single-bacterium fermentation, the preparation method has the advantages that the compound microbial food gum prepared by a mixed-bacterium fermentation 'one-step method ' has a simple process flow, and can be used for promoting the microbial fermentation and increase gum yield. The food gum can improve the gel performance of gellan gum, and can be used for preparing gel without adding cations in the gelling process.

The invention provides an induced sphingomonas paucimobilis and belongs to the field of gellan gum preparation. The induced sphingomonas paucimobilis is collected in China General Microbiological Culture Collection Center, which is located in Institute of Microbiology of Chinese Academy of Sciences, 3#, No.1 Yuan, Western Beichen Road, Chaoyang District, Beijing, with the collection number of CGMCC No.10341 on January 12, 2015. The induced sphingomonas paucimobilis having passage stability can be obtained by inducing the sphingomonas paucimobilis as a starting bacterial strain twice. The induced sphingomonas paucimobilis has passage stability; after the induced sphingomonas paucimobilis is fermented and cultured, the gum-yielding rate of the induced sphingomonas paucimobilis can be 16.25g/L, which is increased by more than 25% in contrast with the gum-yielding rate of the non-induced sphingomonas paucimobilis.

The invention provides a strain which is capable of producing exopolysaccharides with high yield and is separated and screened from leaves of kumquat in Shunchang County, Nanping City, Fujian Province. The strain is a Sphingomonas paucimobilis strain FJAT-10625. The invention further discloses a method for preparing exopolysaccharides from the Sphingomonas paucimobilis strain FJAT-10625. The strain and the method have the advantages as follows: one new Sphingomonas paucimobilis strain FJAT-10625 is provided, and the yield of exopolysaccharides prepared from the strain is higher, so that the source of exopolysaccharide production strains is increased; besides, the process for preparing exopolysaccharides from the Sphingomonas paucimobilis strain FJAT-10625 is simple and easy to operate.

The invention belongs to the technical field of rubber wastewater treatment, and particularly relates to a water purifier adopted in nitrile rubber production and a wastewater treatment method. The water purifier is composed of a solid adsorption material, a microorganism water purifier and an enzymic preparation. The solid adsorption material is prepared from, by weight, 10-25 parts of medical stone, 10-20 parts of slag and 5-20 parts of polyacrylamide. The microorganism water purifier is prepared from, by weight, 0.5-2.0 parts of sphingomonas paucimobilis powder, 0.5-1.5 parts of sulfate reducting bacterium powder, 0.2-1.0 part of rhodococcus ruber powder and 0.5-1.5 parts of paracoccus aminovorans powder. The enzymic preparation is prepared from, by weight, 0.05-0.1 part of thioesterase, 0.04-0.15 part of aldehyde dehydrogenase, 0.03-0.2 part of mannose and 0.02-0.1 part of lipoxygenase. The solid water purifier, the composite microbial agent and the enzymic preparation act on wastewater together, secondary pollution caused by chemical treatment is avoided, sewage discharge is reduced, and the quality of wastewater is improved; by the adoption of the method, CODcr discharge meets the national standard.