13 results about "Sphingomonas sp." patented technology

The invention belongs to the technical field of environmental biology, and particularly relates to a prothioconazole methyltransferase gene proS38 and application thereof. The prothioconazole methyltransferase proS38 gene in the Sphingomonas sp.AJ-1 strain and the prothioconazole methyltransferase which is a protein expression substance thereof are found through analysis, screening, identification and verification, the prothioconazole methyltransferase proS38 gene and the prothioconazole methyltransferase which is a protein expression substance thereof are proved to have efficient and specific degradation catalysis capability on the existing pesticide prothioconazole which is used in a large amount, and a foundation is laid for biodegradation of prothioconazole.

The present invention relates to a use of a Sphingomonas sp. microorganisms, particularly Sphingomonas olei, for promoting hair growth or preventing hair loss. A composition comprising a Sphingomonas sp. microorganism (particularly Sphingomonas olei) or a lysate, culture medium, or extract thereof enhances the growth rate of hair length and increases the expression of growth promotion-related factors of hair follicle cells, and thus is useful for promoting hair growth or preventing hair loss.

Therapeutic compounds and their use in the prevention and treatment of disease are disclosed. The compounds include one or more materials selected from algal biomass / supernatants (including both algae and bacteria), bacterial biomass, and isolated and purified compounds, as well as specific active sites or structures on those compounds. Therapeutic compounds derived from bacteria that appear to selectively alter one or more TLR pathways and the use of such compounds in the prevention and treatment of disease in both animals and humans. The bacteria are selected from the group consisting of Algoriphagus sp., Bosea sp., Brevundimonas sp., Desulfovibrio sp., Microbacterium sp., Sphingomonas sp., and Variovorax sp. The disclosed compounds and methods of treatment find particular use in treating diseases in poultry, particularly in the prevention and treatment of coccidiosis.

The invention discloses sphingomonas CCSM001 separated from human skin and capable of improving skin health and a metagen thereof, and belongs to the field of microbial technology and medicine. The sphingomonas CCSM001 provided by the invention has a good barrier function repairing effect and application prospect in the aspect of external use, and is specifically shown in that the activity of damaged HaCaT cells is improved in vitro, the gene level expression of FLG, IVL, AQP3, ZO-1, Occludin and CLDN is improved in the aspects of functional protein and structural protein after damage. The metagen prepared from the sphingomonas can be used for preparing cosmetics or medicines for repairing skin barriers, and has a huge application prospect.

The invention relates to the technical field of sewage treatment, in particular to aerobic denitrifying bacteria and application thereof. The bacterial strain is monomonas stutzeri C3, the bacterial strain is preserved in Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC No.66733, and the 16S rDNA sequence of the bacterial strain is as shown in SEQ ID NO.1. The invention further discloses a preparation method of the bacterial strain. The invention relates to application of aerobic denitrifying bacteria in sewage treatment, in particular to application in treatment of sewage of a stainless steel plant. The strain disclosed by the invention is used for carrying out denitrification treatment on sewage in an aerobic environment, and nitrate nitrogen, nitrate nitrogen and COD (Chemical Oxygen Demand) in the sewage can be efficiently removed.

The invention belongs to the technical field of microorganisms, and particularly relates to sphingomonas fluorescens and application thereof in lignin degradation. The invention discloses a sphingomonas fluorescens H39, the preservation number of the sphingomonas fluorescens H39 is CCTCC (China Center for Type Culture Collection) No: M 2025936, and the sphingomonas fluorescens H39 is preserved in the China Center for Type Culture Collection on April 29, 2025. The strain not only has remarkable peroxidase activity (85.34 U / mL), but also can stably grow under the condition of multiple nitrogen sources (such as ammonium sulfate, peptone, yeast extract and the like), the culture period is short (3-5 days), and the lignin degradation rate of agricultural wastes such as corn straw and the like is remarkably improved. The invention finds that a new microbial resource is provided for biodegradation of lignin, and resource utilization of agricultural wastes and development of environmental protection industry are expected to be promoted.

This invention provides the application of a uterine-derived Sphingomonas DS-28 strain and its metabolites in the treatment of endometritis, relating to the field of microbial technology. The Sphingomonas DS-28 strain provided by this invention possesses uterine colonization ability, good niche adaptability, and significant efficacy in treating Escherichia coli-induced endometritis. Animal experiments show that the preparation of this strain can effectively alleviate uterine redness and swelling, reduce pathological damage, and significantly reduce [the condition / progression]. TNF-alpha , IL-6 , IL-8 It inhibits gene expression and suppresses the NF-κB signaling pathway, exhibiting superior anti-inflammatory and tissue repair effects compared to other Sphingomonas strains C3-25.1, C3-41.1, and C3-42.1. Furthermore, the metabiotic preparation of this strain can also alleviate Escherichia coli-induced endometritis in mice, improve uterine redness and swelling, and reduce pathological damage.

The invention belongs to preparation of Sanzan gum, and particularly relates to a preparation method of easily purified Sanzan gum fermentation liquor. The method comprises the following steps: inoculating sphingomonas with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.19480 and the Latin name of Sphingomonas sp. Into a sterile fermentation culture medium, and carrying out aerated fermentation; the carbon source of the fermentation medium adopts one or a combination of glucose and sucrose, the inorganic salt adopts one or a combination of potassium dihydrogen phosphate and sodium dihydrogen phosphate, and ammonia water is adopted to adjust the fermentation medium and control the pH value in aerated fermentation to be 6.5-7.5. The method solves the problems of more nitrogen source residues, high ash content, low gum production rate and the like in the prior art, and has the advantages of easiness in purification, high gum production rate, low nitrogen source residues, low ash content and the like.

The application provides a strain of sphingomonas which can degrade phthalate, a bacterial combination and a composite microbial agent and application thereof, and belongs to the technical field of microorganisms. The application provides a strain of sphingomonas which can degrade phthalate, WXX-05, which can simultaneously degrade multiple phthalates. The application also provides a bacterial combination, which comprises the sphingomonas WXX-05 and a new species of rhizomucor WXX-04. The new species of rhizomucor WXX_04 strain and the sphingomonas WXX_05 strain can simultaneously degrade multiple phthalates. The bacterial combination of the application has strong adaptability to the environment, can effectively degrade multiple phthalates, and can improve the phthalate pollution remediation efficiency of soil by being applied to phthalate-polluted farmland soil.

The invention belongs to the field of microorganisms, and particularly relates to a complex microbial inoculant for overcoming continuous cropping obstacles of pinellia ternate and application of the complex microbial inoculant. The active ingredients of the composite bacterial agent are composed of sphingomonas kungheensis JN21 (CGMCC (China General Microbiological Culture Collection Center) No. 36292) and bacillus fruticosa F16 (CGMCC No. 36291), and the active ingredients of the composite bacterial agent are composed of sphingomonas kungheensis JN21 (CGMCC No. 36292) and bacillus fruticosa F16 (CGMCC No. 36291). The two strains can effectively degrade the key continuous cropping autotoxic substance p-hydroxybenzoic acid of pinellia ternate. When the complex microbial inoculant is applied to continuous cropping soil of pinellia ternate, the content of p-hydroxybenzoic acid in the soil is remarkably reduced, the circulation of carbon, nitrogen and sulfur in the soil is promoted, and particularly, the abundance of hydrogen sulfide generation pathway genes is promoted, so that continuous cropping obstacles of pinellia ternate are relieved or prevented. The invention provides an effective microbial solution for solving the continuous cropping obstacle problem of pinellia ternate.

The invention relates to the technical field of microorganisms, and discloses a novel sphingobacterium sp. And application thereof, the novel sphingobacterium sp. Is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 67028. The invention also provides a biological detoxication agent containing the preserved strain and a preparation method of the biological detoxication agent. In addition, the invention also provides an application of the strain or the biological detoxication agent in degrading ochratoxin A, and an application of the strain or the biological detoxication agent in inhibiting aspergillus ochraceus growth and toxin production.

The invention provides application of uterus-derived sphingomonas DS-28 and a metagen thereof in treatment of endometritis, and relates to the technical field of microorganisms. The sphingomonas DS-28 provided by the invention has uterus colonization ability and good ecological niche adaptability, and also has a remarkable curative effect in treatment of Escherichia coli induced endometritis. Animal experiments show that the strain preparation can effectively relieve red and swollen uterus, relieve pathological damage, remarkably reduce gene expression of TNF-alpha, IL-6 and IL-8 and inhibit an NF-kappa B signal channel, and the anti-inflammatory and tissue repair effects of the strain preparation are superior to those of other sphingomonas strains C3-25.1, C3-41.1 and C3-42.1. Meanwhile, the metastatic preparation of the strain can also relieve mouse escherichia coli endometritis, improve red and swollen uterus and relieve pathological injury.

This invention belongs to the field of gene editing technology, specifically relating to a scarless gene editing system for *Sphingomonas* and its application. The scarless gene editing system comprises a first inducing plasmid, a donor plasmid, and a second inducing plasmid. The first inducing plasmid includes the following elements: the EXO gene, the Gam gene, the Beta gene, a tetracycline resistance gene, and replicon 3233. The donor plasmid includes an exogenous DNA fragment for homologous recombination capable of amplifying upstream and downstream homologous arms of the target gene, an FRT fragment, and a chloramphenicol resistance gene. The second inducing plasmid includes the following elements: the FLP gene, a bleomycin resistance gene, and replicon 3233. Based on this scarless gene editing system, this invention knocks out the *Sphingomonas* 1535 gene, achieving an engineered strain capable of producing zeaxanthin, and further knocks out the 516 gene to increase zeaxanthin yield.