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Welan gum high-producing strain sphingol monad TJA 3-1 and method and application thereof for producing welan gum

A technology of sphingomonas and high-yield strains, which is applied in the field of microorganisms, can solve the problems of low gelatin content in fermentation broth, single fermentation strains, high cost of fermentation raw materials, etc., and achieve good temperature resistance

Inactive Publication Date: 2019-07-09
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Welan gum is the earliest product developed by CPkelco in the United States. Domestic Hebei Xinhe Biochemical and other companies are also engaged in the production and development of Welan gum. However, the existing Welan gum fermentation strains are single, mostly Sphingomonassp.ATCC 31555 and Its variant has problems such as high cost of fermentation raw materials, low fermentation yield, and less gum content in the fermentation broth.

Method used

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  • Welan gum high-producing strain sphingol monad TJA 3-1 and method and application thereof for producing welan gum
  • Welan gum high-producing strain sphingol monad TJA 3-1 and method and application thereof for producing welan gum
  • Welan gum high-producing strain sphingol monad TJA 3-1 and method and application thereof for producing welan gum

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Embodiment 1

[0040] A strain of Sphingomonas TJA 3-1 with a high yield of Welan gum, named TJA 3-1, taxonomic name Sphingomonas sp., preservation number: CGMCC No.15057, preservation date: December 13, 2017 , No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, preservation unit: General Microbiology Center of China Committee for the Collection of Microbial Cultures.

[0041] Wherein, the Sphingomonas TJA 3-1 is isolated from sandy soil in Ongniud Desert, Inner Mongolia.

[0042] Wherein, the characteristic of the taxonomic identification of said strain Sphingomonas TJA 3-1 is: Gram-negative bacillus, cell size is 0.6-1.0um * 1.2-2.5um, forms yellow, Sticky colonies ( figure 1 ), the optimum growth temperature is 28°C, the optimum growth pH is 6.0, the NaCl tolerance range is 0-0.3%, oxidase, catalase, nitrate reduction and escin hydrolysis are positive, methyl red and V-P test , starch hydrolysis, gelatin hydrolysis and indole production were negative.

[0043] Take 5 mL o...

Embodiment 2

[0052] A strain of Sphingomonas TJA 3-1 with a high yield of Welan gum, named TJA 3-1, taxonomic name Sphingomonas sp., preservation number: CGMCC No.15057, preservation date: December 13, 2017 , No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, preservation unit: General Microbiology Center of China Committee for the Collection of Microbial Cultures.

[0053] Wherein, the Sphingomonas TJA 3-1 is isolated from sandy soil in Ongniud Desert, Inner Mongolia.

[0054] The relevant steps are as follows:

[0055] 1) Isolation and purification of bacterial strains:

[0056] Collect sandy soil samples from the Ongniud Desert in Inner Mongolia, take 10g of soil samples, add sterilized saline solution to vortex and oscillate, take the supernatant after standing still, dilute with sterile normal saline, take 100uL sample solution and spread on Screen the surface of medium (g / L: sucrose 10, peptone 3, sodium chloride 0.5, beef extract 1, yeast extract 1, pH 6.5). Cultiv...

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Abstract

The invention relates to welan gum high-producing strain sphingol monad TJA 3-1. The name of the welan gum high-producing strain sphingol monad TJA 3-1 is TJA 3-1, the classification name is Sphingomonas sp., the preservation number is CGMCC No.15057, the preservation date is 13th December, 2017, 3 yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation institution is China General Microbiological Culture Collection Center. The strain can be fermented and produced with low-cost molasses as a raw material, the viscosity of fermentation liquor reaches 10500 mPa.s, the yield of the welan gum reaches 21 g / L, and still has good temperature resisting performance at the temperature of 160 DEG C, and a new path for producing the welan gum is provided.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to a high-yielding Welan gum strain Sphingomonas TJA 3-1 and a method and application for producing Welan gum. Background technique [0002] Many microorganisms in nature can synthesize extracellular polysaccharides, which adhere to the cell surface or secrete into the culture medium in the form of amorphous mucus to protect the microorganisms. The polysaccharides synthesized by some microorganisms have become important biotechnology products. Known as microbial glue, it is widely used in chemical, food and other industries. Sphingomonas is an important source of microbial species that can synthesize microbial glue. Some strains of this genus can synthesize polysaccharides with similar main chain structures, which are collectively called sphingans. Including gellan gum (Gellan) synthesized by Sphingomonas elodea ATCC 31461, Welan gum (Welan) synthesized by Sphingomonas sp.ATCC 315...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/01
CPCC12P19/04C12N1/205C12R2001/01
Inventor 黄海东吴一凡李晓雁杨红澎王新张怡
Owner TIANJIN AGRICULTURE COLLEGE
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