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High-performance welan gum strain and application thereof

A high-performance technology of Brunei glue, applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of backward quality and lack of large scale, and achieve high glue yield, short growth cycle and comprehensive performance excellent effect

Active Publication Date: 2015-04-22
INNER MONGOLIA RAINBOW BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many scientific research institutions and related companies in China have already realized this point and conducted extensive research on it. However, its core technology has been firmly controlled by American companies, so the domestic Brunei rubber industry has been in the initial stage of exploration. It has formed a large scale, and its quality is far behind the standards of American companies.

Method used

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  • High-performance welan gum strain and application thereof
  • High-performance welan gum strain and application thereof
  • High-performance welan gum strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Obtaining High Performance Brunei Gum Strain

[0026] 1. The activation of the original strain: the original strain is Sphingomonas (Sphingomonas Species.ATCC31555), which is purchased from Jiangnan University. Transfer it to the slant medium using aseptic technique in the bacterial operation bench, and cultivate it at 30°C for 72 hours; the formula of the slant medium is: 1% sucrose, 2% soybean peptone, 0.5% yeast extract, 2% agar, and the rest is Distilled water, pH control: 7.0-7.2;

[0027] 2. The state after cultivation on the slope: the bacterial lawn is yellow, thin, and the surface is wet. After further cultivation, the color deepens and the surface is dry;

[0028] 3. Re-cultivation on the slant: transfer the slant culture after 72 hours to the slant again for secondary culture at 30°C for 72 hours;

[0029] 4. The state after the secondary culture on the slope: the bacterial lawn is yellow, thick, and the surface is wet. After further cultivation, the color ...

Embodiment 2

[0040] The original strain and Sphingomonas W-1 were cultured in shake flasks respectively, and the culture conditions and culture medium were the same as in Example 1;

[0041] Group 1: Shake flask culture for 30 hours to obtain the original strain and Sphingomonas W-1 bacteria liquid, calculate the concentration of the bacteria, inoculate the same amount of bacteria into the fermentation medium for fermentation culture, use a shaker culture for fermentation culture, temperature 30 ℃, rotating speed: 150 rev / min, fermentation culture 70h; fermentation medium: glucose 20g / L, ammonium nitrate 0.5g / L, yeast powder 0.4g / L, KH 2 PO 4 4g / L, K 2 HPO 4 3g / L,FeSO 4 0.0025g / L, 1ml / L of comprehensive trace element solution, pH 7.0, sterilized at 121°C for 20 minutes; among them, the comprehensive trace element solution contains 0.5% magnesium sulfate, 0.1% manganese sulfate, and 0.25% zinc sulfate;

[0042] Group 2: Shake flask culture for 30 hours to obtain the original strain and ...

Embodiment 3

[0050] The original strain and Sphingomonas W-1 were cultured in shake flasks respectively, and the culture conditions and culture medium were the same as in Example 1;

[0051] Group 1: Shake flask culture for 30 hours to obtain the original strain and Sphingomonas W-1 bacteria liquid, calculate the concentration of the bacteria, inoculate the same amount of bacteria into the fermentation medium for fermentation culture, use a fermenter for fermentation culture, and the temperature is 30°C, ferment for 70 hours; fermentation medium: glucose 20g / L, ammonium nitrate 0.5g / L, yeast powder 0.4g / L, KH 2 PO 4 4g / L, K 2 HPO 4 3g / L, FeSO40.0025g / L, 1ml / L of comprehensive trace element solution, pH 7.0, sterilized at 121°C for 20 minutes; among them, the comprehensive trace element solution contains 0.5% magnesium sulfate, 0.1% manganese sulfate, and 0.25% zinc sulfate, of which , the dissolved oxygen in the fermentation process is 10%;

[0052] Group 2: Shake flask culture for 30 ...

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Abstract

The invention belongs to the field of production of welan gum and particularly relates to a high-performance welan gum strain and application thereof. The high-performance welan gum strain is collected in the China General Microbiological Culture Collection Center (CGMCC) on November 3, 2014 as the name of (Sphingomonas sp.) W-1 with the collection number of CGMCC No.9919. The high-performance welan gum strain provided by the invention is prepared by carrying out ultraviolet mutagenesis screening on an original strain. Compared with the original strain, the high-performance welan gum strain has short growth cycle. The welan gum obtained by fermenting by using the welan gum strain has high yield, high viscosity, good salt resistance and good high-temperature resistance. The invention further provides application of the high-performance welan gum strain to fermentation of welan gum.

Description

technical field [0001] The invention relates to the field of Brunei gum production, in particular to a high-performance Brunei gum strain and its application. Background technique [0002] Brunei gum, as a new type of microbial polysaccharide, has many excellent and unique physical and chemical properties, and has a wide range of applications in petroleum, chemical, food, cosmetics and other fields. Among them, Brunei gum has unique suspension, stability, thickening and other properties, and is widely used in drilling mud formulations, construction concrete and mortar formulations, coatings and self-leveling mortar formulations, etc. Compared with xanthan gum, it has Unique advantages. Many scientific research institutions and related companies in China have already realized this point and conducted extensive research on it. However, its core technology has been firmly controlled by American companies, so the domestic Brunei rubber industry has been in the initial stage of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/01
CPCC12P19/04C12N1/205C12R2001/01
Inventor 古立谦
Owner INNER MONGOLIA RAINBOW BIOTECH CO LTD
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