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A kind of high-performance Brunei gum bacterial strain and application thereof

A Brunei gum, high-performance technology, applied in bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of backward quality, no large scale, etc., to achieve high glue yield, short growth cycle, comprehensive performance excellent effect

Active Publication Date: 2017-06-30
INNER MONGOLIA RAINBOW BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many scientific research institutions and related companies in China have already realized this point and conducted extensive research on it. However, its core technology has been firmly controlled by American companies, so the domestic Brunei rubber industry has been in the initial stage of exploration. It has formed a large scale, and its quality is far behind the standards of American companies.

Method used

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  • A kind of high-performance Brunei gum bacterial strain and application thereof
  • A kind of high-performance Brunei gum bacterial strain and application thereof
  • A kind of high-performance Brunei gum bacterial strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Obtaining high-performance Brunei gum strains

[0026] 1. Activation of the original strain: The original strain is Sphingomonas Species (ATCC31555), which is purchased from Jiangnan University. The strain can also be purchased commercially; the original strain is a freeze-dried powder, which is sterile In the operating table, it was transferred to the slant culture medium by aseptic operation technology, and cultured at 30℃ for 72 hours; the formula of the slant culture medium: sucrose 1%, soy peptone 2%, yeast extract 0.5%, agar 2%, the rest was distilled water , PH control: 7.0-7.2;

[0027] 2. The state after cultivating the inclined plane: the moss is yellow, thin, and the surface is moist. After further cultivation, the color becomes darker and the surface is dry;

[0028] 3. Slope re-cultivation: The slant culture after 72h is transferred to the slant again for secondary cultivation, the culture temperature is 30℃, and the time is 72h;

[0029] 4. The state of the incli...

Embodiment 2

[0040] The original strain and Sphingomonas W-1 were cultured in shake flasks, and the culture conditions and culture medium were the same as in Example 1;

[0041] Group 1: Shake flask culture for 30 hours to obtain the original strain and Sphingomonas W-1 bacterial solution, calculate the concentration of the bacteria, inoculate the same amount of inoculation to the fermentation medium for fermentation culture, the fermentation culture uses a shaker culture, temperature The temperature is 30℃, the rotation speed is: 150 rpm, the fermentation culture is 70h; the fermentation medium: glucose 20g / L, ammonium nitrate 0.5g / L, yeast powder 0.4g / L, KH 2 PO 4 4g / L, K 2 HPO 4 3g / L, FeSO 4 0.0025g / L, 1ml / L of integrated trace element solution, pH 7.0, sterilized at 121°C for 20 minutes; among them, the integrated trace element solution contains 0.5% magnesium sulfate, 0.1% manganese sulfate, and 0.25% zinc sulfate;

[0042] Group 2: Shake flask culture for 30 hours to obtain the original st...

Embodiment 3

[0050] The original strain and Sphingomonas W-1 were cultured in shake flasks, and the culture conditions and culture medium were the same as in Example 1;

[0051] Group 1: Shake flask culture for 30 hours to obtain the original strain and Sphingomonas W-1 bacterial liquid, calculate the bacterial concentration, inoculate the same amount of inoculation into the fermentation medium for fermentation culture, and use the fermentation tank for fermentation culture at a temperature of 30℃, fermentation culture for 70h; fermentation medium: glucose 20g / L, ammonium nitrate 0.5g / L, yeast powder 0.4g / L, KH 2 PO 4 4g / L, K 2 HPO 4 3g / L, FeSO40.0025g / L, 1ml / L of integrated trace element solution, pH 7.0, sterilized at 121°C for 20 minutes; among them, the integrated trace element solution contains 0.5% magnesium sulfate, 0.1% manganese sulfate, and 0.25% zinc sulfate. , The dissolved oxygen in the fermentation process is 10%;

[0052] Group 2: Shake flask culture for 30 hours to obtain the or...

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Abstract

The invention relates to the field of Brunei gum production, in particular to a strain of Brunei gum with high performance and its application. A high-performance Brunei gum strain, preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on November 03, 2014, the preservation number is CGMCC No.9919, and the preservation name is Sphingomonas sp. W-1. The high-performance Brunei gum strain provided by the present invention is obtained by screening the original strain by ultraviolet mutagenesis. Compared with the original strain, the growth period of the strain is shorter, and the rubber Brunei gum produced by fermentation has a high gel yield, and the obtained Brunei gum is viscous Higher temperature, more salt and high temperature resistance. The present invention also provides the application of the high-performance Brunei gum strain in the fermentation of Brunei gum. The high-performance Brunei gum strain provided by the invention has a high yield of Brunei gum produced by fermentation, and the obtained Brunei gum has higher viscosity, more salt resistance and higher temperature resistance.

Description

Technical field [0001] The present invention relates to the production field of Brunei gum, in particular to a high-performance Brunei gum strain and its application. Background technique [0002] As a new type of microbial polysaccharide, Brunei gum has many excellent and unique physical and chemical properties and has a wide range of applications in petroleum, chemical, food, cosmetics and other fields. Among them, Brunei gum has unique suspending, stabilizing and thickening properties, and is widely used in drilling mud formulations, construction concrete and mortar formulations, coatings and self-leveling mortar formulations. Compared with xanthan gum, it has Unique advantage. Many domestic scientific research institutions and related companies have already recognized this point and conducted extensive research on it, but its core technology has been firmly controlled by American companies, so the domestic Brunei rubber industry has been in the initial stage of exploration. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/01
CPCC12P19/04C12N1/205C12R2001/01
Inventor 古立谦
Owner INNER MONGOLIA RAINBOW BIOTECH CO LTD
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