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Mangrove plant rhizosphere growth promoting azotobacter (DZY-N56) and uses thereof

A technology for promoting growth of mangrove plants and rhizosphere, applied in the biological field, can solve problems such as low survival rate, and achieve the effect of improving productivity and promoting growth.

Inactive Publication Date: 2010-04-14
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the survival rate of introduced strains in our country's mangroves is low, and there are also various hidden dangers caused by foreign species invasion

Method used

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  • Mangrove plant rhizosphere growth promoting azotobacter (DZY-N56) and uses thereof
  • Mangrove plant rhizosphere growth promoting azotobacter (DZY-N56) and uses thereof
  • Mangrove plant rhizosphere growth promoting azotobacter (DZY-N56) and uses thereof

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Experimental program
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Effect test

Embodiment Construction

[0029] 1. Materials and methods

[0030] 1. Materials

[0031] 1.1 Soil sample collection

[0032] The samples were collected from the rhizosphere sediments of Bruguiera gymnorhiza in the mangrove area along the Hongsha River in Sanya City, Hainan Province. After the samples were collected, they were put into sterilized sealed polyethylene bags and brought back to the laboratory for low temperature storage.

[0033] 1.2 Medium

[0034] Ashby Broth: Mannitol 10.0g, KH 2 PO 4 ·H 2 O 0.2g, MgSO 4 ·7H 2 O 0.2g, NaCl 0.2g, CaSO 4 0.1g, CaCO 3 5.0g, 1000mL deionized water, pH 7.0.

[0035] Ashby solid medium: Ashby liquid medium + 2.2% agar.

[0036] to improve Nitrogen-free liquid medium: sucrose 10g, malic acid 5.0g, K 2 HPO 4 ·H 2 O 0.1g, KH 2 PO 4 ·H 2 O 0.4g, MgSO 4 ·7H 2 O 0.2g, NaCl 0.1g, NaCl 2 MoO 4 ·H 2 O 0.002g, FeCl30.01g, deionized water 1000ml, pH 7.0.

[0037] LB medium: yeast extract 5g, tryptone 10g, NaCl 10g, agar 1-2%, deionized water 1000m...

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Abstract

The invention provides redwood plant rhizosphere promoting azotobacteria (DZY-N56) and an application thereof. The bacteria is Sphingomonas sp. DZY-N56, is sieved and separated from a redwood plant rhizosphere sediment sample in a tropical mangrove zone, contains a nif gene nifH and 16S rDNA as shown by SEQ1, and is preserved in China Center for Type Culture Collection with a preservation No. of CCTCC NO: M207120. The strain has high biological nitrogen fixation activity, and can obviously promote plant growth by using microbial inoculum which contains the bacteria for inoculation of redwood plants such as mangrove, Rhizophora stylosa, bruguiera gymnorrhiza and so on, thereby the strain can be applied to prepare biological azotobacterin for promoting the growth of the mangrove plants.

Description

Technical field: [0001] The invention belongs to the field of biological technology, and in particular relates to a mangrove rhizosphere nitrogen-fixing bacterium having a good growth-promoting effect on mangrove plants and an application thereof for preparing biological nitrogen-fixing bacterial fertilizer. Background technique: [0002] Mangroves are woody plant communities naturally distributed in the intertidal zone of tropical and subtropical coasts, covering about 60-75% of tropical and subtropical coasts, playing an important role in maintaining the ecological balance of bays and estuaries, and are an important part of marine productivity (Holguin et al., 2001), considered to be a highly productive ecosystem. The ecosystem has high levels of organic matter (100 TgC per year in global litter), providing organic matter to adjacent coastal waters and associated biome habitats (Holguin et al., 2001; Lugomela and Bergman, 2002). However, mangrove ecosystems are poor in in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N15/31C05F11/08C12R1/01
Inventor 董俊德杨志浩张燕英杨斌凌娟田新鹏
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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