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Microbial preparation for treating high-grease restaurant wastewater and application of microbial preparation

A microbial agent and microbial technology, applied in the field of environmental biology, can solve the problems of low lipase activity, increased sewage treatment process, low grease removal efficiency, etc., achieve good application prospects, and remove high grease pollution effects

Inactive Publication Date: 2016-01-06
NINGBO BLUE ENVIRONMENT SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This not only needs to add additional corresponding equipment, but also increases the technological process of sewage treatment, and the lipase used must be immobilized to improve its enzymatic properties, which will inevitably increase the cost of sewage treatment.
Adding lipase-producing microorganisms directly into activated sludge can save the trouble of adding equipment and increasing the process flow. However, due to the low activity and poor properties of lipase in general lipase-producing microorganisms, the removal of grease in sewage treatment Low efficiency, long sewage treatment cycle

Method used

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  • Microbial preparation for treating high-grease restaurant wastewater and application of microbial preparation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 A method for removing pollutants in sewage

[0024] The catering sewage of a hotel was taken as the research object. To achieve the determination of the corresponding oil and COD content.

[0025] Sewage pretreatment, the catering sewage is passed through a solid-liquid separator for solid-liquid separation to remove large solid particles, then the liquid enters the sedimentation tank and settles for 4 hours, and then the liquid is passed through a round hole filter to remove solid flocs, round holes The hole diameter of the filter screen is 0.1mm.

[0026] Biological oxidation: the liquid through the round hole filter screen enters the biological reaction tank, adjusts the pH to 7, and adds 15 grams of compound bacterial agent per cubic meter of liquid, once a day, continuously for one week, and finally stands for 3 day, drain the liquid.

[0027] Above-mentioned mixed bacterium liquid is mixed by the raw material bacterium of following weight portion: ...

Embodiment 2

[0034] The acquisition of embodiment 2 transgenic bacteria

[0035] Using DNAMAN software analysis, the nucleotide sequence YZ gene was synthesized according to the amino acid sequence WP_011915015.1 whole gene, the target fragment was obtained by amplification, and the target gene YZ was obtained by PCR amplification (at the same time, the corresponding amino acid mutation position was obtained by multiplex PCR Points 65L / S, 71R / T, 83T / V, 119C / M, 130T / Q, 144G / S, 179H / I, 192F / G, 241G / L, 253D / S, 261F / V were introduced into the gene sequence , so as to obtain different mutant genes), the PCR product was double-digested with restriction endonucleases, connected to the expression vector PWB980 that had also been double-digested, and the successfully verified recombinant plasmid was transformed into the Bacillus pumilus ATCC27142 BP-YZ, a genetically engineered bacterium that degrades high-fat sewage, was obtained.

Embodiment 3

[0036] Implementation example 3: Verification of the sewage treatment effect of Bacillus pumilus genetically engineered bacteria and other bacterial agents

[0037] According to the method of Example 1, the corresponding catering sewage treatment experiment was carried out, and the sewage and the sewage of Example 1 belonged to the same batch, and had the same concentration of grease. Wherein each component composition of bacterial agent is identical with embodiment 1.

[0038]The genetically engineered bacteria with different mutation sites were used to remove grease. Through experiments, it was found that 65L / S, 71R / T, 83T / V, 119C / M, 130T / Q, 144G / S, 192F / G, 241G / L, 253D / S, and 261F / V mutants all had significantly enhanced effects compared with the original strain. The total processing time is not more than 4 days, and the results are as follows:

[0039] The sewage is the same batch of sewage, so as to ensure the same conditions.

[0040]

[0041] It can be seen from ...

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Abstract

The invention relates to a microbial preparation for treating high-grease restaurant wastewater and application of the microbial preparation, discloses a method for removing pollutants from urban restaurant wastewater and belongs to the field of wastewater treatment. According to the method, a complex microbial inoculant is used and comprises 15 parts of rhodococcus, 10 parts of thiobacillus denitrificans, 6 parts of sphingomonas sp. and 30 parts of bacillus pumilus. The method has the advantages that microorganisms are mainly used for treating the pollutants, the grease pollutants in the restaurant wastewater can be removed effectively, input cost is low, and accordingly, the method is promising in application prospect.

Description

technical field [0001] The invention relates to a microbial preparation applied to the treatment of high-fat restaurant sewage and belongs to the technical field of environmental biology. Background technique [0002] With the development of society and the improvement of people's living standards, the catering industry is increasingly prosperous. The sewage discharged from the catering industry generally contains oil and chemical substances left by the detergent used to clean the oil. If such sewage is discharged directly, it will cause environmental pollution, because the oily substances in this sewage are easy to adhere to the drainage pipes , Long-term accumulation is likely to cause pipeline blockage. At present, the wastewater generated by catering enterprises and merchants generally enters the urban sewage treatment plant directly through the drainage system. The urban sewage treatment plant is equipped with a biochemical treatment system to decompose heavy metals, n...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N1/20C12N9/20C02F3/34C02F9/14C12R1/07C12R1/01
Inventor 冯国亮张正剑
Owner NINGBO BLUE ENVIRONMENT SCI & TECH CO LTD
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