46results about How to "Extensive culture conditions" patented technology

Production of gama-polyglutamic acid by fermentation is carried out by: culturing bacillus subtills zju-7 strain, i.e., CGMCC No. 1250, in medium with carbon and nitrogen sources, glutamic acid, MgSO4, NaCl and K2HPO4 to obtain ferment liquid, and making it to pass organic solvent settlement and dialysis. The process is easy, yields highly, and costs low with quality products.

The invention provides a composite bacillus preparation containing three strains, a preparation method of the composite bacillus preparation and application of the composite bacillus preparation to ecological breeding, and belongs to the technical field of microorganism and feed additives. Bacillus coagulans, butyric acid clostridia and bacillus megaterium are used as fermentative strains respectively, and large-scale, low-cost and high-density fermentation production of spores is conducted through step-by-step magnification; three live bacterium preparations are prepared by mixing bacterial sludge with the spores and dry starch; the three live bacterium preparations are matched according to the equal weight ratio, the composite bacillus preparation is obtained, and the concentration of the spores reaches more than 1*108-109 cfu/g; the composite bacillus preparation serves as a microorganism and feed additive to be added to farmed animal feed according to the mass ratio ranging from 0.02% to 0.5%. The composite bacillus preparation is simple and stable in process, low in cost, high in spore content and environment tolerance, easy to store and high in survival rate; the purposes of composition and synergism are achieved through the collaborative supplementary effects of different bacilli in the aspect of micro-ecology adjustment, growth of farmed animals is promoted, the feed utilization rate and feed rewards are increased, diarrhea is prevented and reduced, and the breeding ecological environment is improved.

The invention relates to an epicoccum nigrum DB3 bacterial strain as well as a preparation and an application thereof. The preparation method comprises the steps of: 1, placing rotten sea grass in an enrichment culture medium, standing and culturing at room temperature, then coating the enrichment culture medium in a separation culture medium, standing and culturing at a temperature of 30 DEG C for 1-4 days to obtain a wild type flax biological treatment bacterial strain; 2, inoculating the bacterial strain obtained from the step 1 into a flax lignin nutrition culture medium, culturing at a temperature of 28 DEG C for 72 hours; and 3, selecting the bacterial strain with the capacity of degrading the lignin from the flax lignin nutrition culture medium to obtain the epicoccum nigrum DB3 bacterial strain. The epicoccum nigrum DB3 bacterial strain can be applied to a process for preparing spinning flax, hemp, jute or red ramie fibers through peroxide degumming. The epicoccum nigrum DB3 bacterial strain has the characteristics of short growth period, low possibility of being polluted, low treatment cost, mild reaction conditions, strong pollution resistance capacity, good heat-resistant performance, no environment pollution and good quality of the treated fibers. The preparation method has a simple process and is suitable for large-scale industrial production.

The invention discloses Streptomyces griseofuscus LS-H1, namely CCTCC M 209211, and a method for preparing epsilon-polylysine and salt thereof by utilizing the strain. The Streptomyces griseofuscus LS-H1 is the strain obtained through screening; and the epsilon-polylysine at the concentration of between 0.7 and 20g/L can be accumulated under optimum conditions by culturing the Streptomyces griseofuscus LS-H1 in a culture medium containing a carbon source and a nitrogen source. After thalluses are removed from a zymotic fluid through centrifugation or filtration, the epsilon-polylysine and the salt thereof are obtained through the separation by an ion exchange method. The method is simple in the culture of the thalluses, and is favorable for industrialized mass production.

The invention discloses application of Bacillus subtilis in high-yield adenosine and acetoin co-production. The Bacillus subtilis is CGMCC No. 4484. Screened strains can be fermented for adenosine production and acetoin co-production by multiple carbon sources and nitrogen sources. Yield of adenosine and acetoin can be evidently increased by adding sodium citrate into fermentation medium. Operation is convenient and simple, culture conditions are extensive, a higher level can be kept on a 5L fermentation tank, and potential of large-scale industrialization is provided.

The invention relates to Escherichia coli and an application thereof in fermentation production of L-tryptophan, the Escherichia coli is Escherichia coli ACTrp104, the Escherichia coli ACTrp104 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is December 3, 2018, and the preservation number of the strain is CGMCC No.16861. The invention further discloses an application of the Escherichia coli in fermentation production of L-tryptophan. The Escherichia coli AC-1042 is screened by combining an ARTP mutation breeding technology and a resistance directional screening technology, and the strain has unique physiological and biochemical characteristics and can be researched and applied in the fields of scientific research, industry and the like.

This invention discloses a method for biologically removing Ca during preparation pf chitin. The method comprises: mixing Aspergillus niger with one of chitin-containing material, saccharide, starch or starch-containing carbohydrate, fermenting in a container equipped with stirrer at 28-40 deg.C, 0.1-0.3 MPa and pH value of 5-7, introducing asepsis wind with a flux of 400-700 m3/h, stirring, terminating fermentation when the pH value is lower than 2.5 and the chitin content is lower than 10%, discharging, solid-liquid separating, washing the solid with water and drying to obtain chitin. The method has such advantages as no need for inorganic strong acid, no secondary pollution, low water and energy consumption, no waste liquid, little wastewater and little pollution. The obtained chitin has high quality. The method can effectively recover and utilize the byproducts, thus has a high profit.

The invention relates to a method for preparing fibrilia by using epicoccum nigrum DB3 strains. The method comprises the following steps: (1) preserving the epicoccum nigrum DB3 strains in a potato dextrose agar medium for cultivation, and adding a freezing buffer solution; (2) inoculating bacteria the amount of which equals to the pick-up amount of an inoculating ring, obtained in the step (1) into the potato dextrose agar medium, and carrying out shaking culture; (3) inoculating the culture medium obtained in the step (2) into a flax lignin fermentation medium, and carrying out shaking culture to obtain a fermentation bacteria liquid; (4) mixing sterilized fiber raw material with the fermentation bacteria liquid obtain in the step (3), carrying out table shaking and removing fermentation liquid to obtain the fibrilia; and (5) mixing the fibrilia with degumming liquid, processing and then rinsing, oiling and drying to obtain the fibrilia. The method in the invention has simple technology, short degumming time and high degumming efficiency, thus being suitable for large-scale industrial production.

The invention relates to a strain of Escherichia coli ACThr1032 and application thereof in fermentation production of L-threonine, and belongs to the field of biotechnology. The strain of Escherichiacoli ACThr1032 has a classification name of namedEscherichia coli, a deposit number of CGMCC No.16144, and a preservation date of July 23, 2018. The Escherichia coli ACThr1032 is cultured, fermented,purified and fermented to prepare L-threonine. The strain stability of Escherichia coli ACThr1032 in the invention is greatly improved, and the strain is used for fermentation production of L-threonine, acid production and conversion have been significantly improved, and breakthroughs have been made in technology, with significant progress. Moreover, the culture condition of the strain is extensive, which greatly reduces the energy consumption and has a good prospect of industrial application.

The invention discloses recombinant bacillus subtilis of exocytosis PNAG polysaccharide. A PNAG polysaccharide synthetase coding gene and expression plasmid are connected, recombinant expression plasmid is constructed, then the recombinant expression plasmid is transferred into bacillus subtilis, and the recombinant bacillus subtilis is obtained. The invention further discloses a construction method of the recombinant bacillus subtilis. A new metabolism synthetase gene is guided into bacillus subtilis, the recombinant bacillus subtilis is successfully constructed, PNAG polysaccharide can be produced through fermentation, the produced exocytosis PNAG polysaccharide can reach 136mg/L, and a base is established for further reforming the bacillus subtilis for producing the PNAG polysaccharidethrough metabolic engineering. The invention further discloses an application of the recombinant bacillus subtilis to preparation of products containing the PNAG polysaccharide through fermentation indifferent fields of foods, medicines and chemical industry. The PNAG polysaccharide produced from recombinant strains can promote growth of probiotics and restrain harmful bacteria, and has good application prospects in many fields.