Streptomyces griseofuscus strain and method for preparing epsilon-polylysine and salt thereof by utilizing same

A technology of Streptomyces tamarus and polylysine, which is applied in the fields of food engineering and fermentation engineering, can solve the problems of ε-polylysine production strains and technical limitations, and achieves the effects of convenient operation and extensive culture conditions

Active Publication Date: 2011-06-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

That is to say, compared with foreign monopoly production, the production of ε-polylysine in China is still subject to many restrictions due to strains and technical reasons.

Method used

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  • Streptomyces griseofuscus strain and method for preparing epsilon-polylysine and salt thereof by utilizing same
  • Streptomyces griseofuscus strain and method for preparing epsilon-polylysine and salt thereof by utilizing same
  • Streptomyces griseofuscus strain and method for preparing epsilon-polylysine and salt thereof by utilizing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Slant medium: 1.0% glucose, 0.2% peptone, 0.1% yeast extract, 1.5% agar, pH 7.5, sterilized at 115°C for 20min.

[0050] Shake flask medium: glucose 5%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.08%, KH 2 PO 4 0.14%, MgSO 4 ·7H 2 O 0.05%, ZnSO 4 ·7H 2 O 0.005%, FeSO 4 ·7H 2 O 0.001%, yeast extract 0.5%, pH 6.8, sterilized at 115°C for 20min.

[0051] The purified CCTCC M 209211 was cultured on a slant medium at 30°C for 7 days, and then a ring of spores of this fungus was placed in the shake flask medium, cultured at 30°C for 72h, and the shaker flask rotated at 200r / min. Finally, the content of ε-polylysine in the fermented liquid was 0.7 g / L.

Embodiment 2

[0053] Fill a 5-liter glass stirred fermenter with 3L of shake flask medium and steam sterilize at 121°C for 15 minutes. CCTCC M 209211 was cultured with seed medium at 30°C for 24h, and the seed medium was the same as the fermentation medium. Insert 240ml of the seed liquid into the cooled fermented liquid, cultivate at 30°C, (ventilation ratio 1:2.5vvm, stirring speed is 350r / min), when 72h, the fermented liquid contains ε-polylysine 0.8g / L.

Embodiment 3

[0055] Same as Example 2, the fermentation process is controlled at pH 3.5, glucose and nitrogen sources are added to maintain a glucose concentration of about 10 g / L, and after 100 hours of fermentation, 20 g / L of ε-polylysine is accumulated in the fermentation broth. Centrifuge the fermentation broth to remove bacteria, adsorb on 732 cation exchange resin on the supernatant, wash with water, elute with ammonia water, concentrate the collected liquid to remove nitrogen, decolorize with activated carbon, continue to concentrate and precipitate with ethanol to obtain ε-polylysine hydrochloride 13.5g. The specific rotation of the product a 25 D =+57.1(c,1H 2 0), the molecular weight measured by SDS-PAGE electrophoresis is 5000Da.

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Abstract

The invention discloses Streptomyces griseofuscus LS-H1, namely CCTCC M 209211, and a method for preparing epsilon-polylysine and salt thereof by utilizing the strain. The Streptomyces griseofuscus LS-H1 is the strain obtained through screening; and the epsilon-polylysine at the concentration of between 0.7 and 20g/L can be accumulated under optimum conditions by culturing the Streptomyces griseofuscus LS-H1 in a culture medium containing a carbon source and a nitrogen source. After thalluses are removed from a zymotic fluid through centrifugation or filtration, the epsilon-polylysine and the salt thereof are obtained through the separation by an ion exchange method. The method is simple in the culture of the thalluses, and is favorable for industrialized mass production.

Description

technical field [0001] The invention belongs to the technical fields of fermentation engineering and food engineering, and specifically relates to a high-yield polylysine microbial strain Streptomyces griseus, which is used in the production of polylysine and its salt and a specific production and preparation method. Background technique [0002] Polylysine is a L-lysine homopolymer synthesized by microorganisms, which is formed by linking ε-amino groups and carboxyl groups through peptide bonds. ε-polylysine has the activity of resisting Gram-positive bacteria, Gram-negative bacteria, fungi and viruses, and is a biological preservative with excellent performance. In addition, it is also used as cosmetics, gene carriers, drug coatings, electronic materials and environmental protection materials. [0003] In 1977, when Sakai Heiichi and Shima Shoji screened a large number of D.P. positive substances (alkaloids), they found a large amount of D.P. substances in the fermentatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P13/00C12R1/465
Inventor 毛忠贵张建华张宏建李树
Owner JIANGNAN UNIV
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