401 results about "Epsilon-Polylysine" patented technology

It has been desired to develop a highly preservative and antibacterial cosmetic composition that can easily be applied to both emulsion and non-emulsion type cosmetics. It has also been desired to develop a method of improving a preservative and/or antibacterial effect(s) of a cosmetic composition comprising polyorganosiloxane-containing epsilon-polylysine and thereby reducing the amount of antibacterial preservative agent to be used. There is provided a cosmetic composition comprising one or a combination of two or more of polyorganosiloxane-containing epsilon-polylysine compounds obtained by reacting epsilon-polylysine with polyorganosiloxane or a physiologically acceptable salt thereof, and polyhydric alcohol.

The invention relates to a compound biological antiseptic and preservation agent for food. The compound biological antiseptic and preservation agent is prepared by evenly mixing pure biological solid preparations or products such as epsilon-polylysine, nisin, natamycin, lysozyme, bacillus subtilis, ascorbic acid, a toona sinensis leaf extract, a water-soluble resveratrol preparation, tea polyphenol and chitosan. The compound biological antiseptic and preservation agent has a strong inhibition effect on spoilage bacteria and pathogenic bacteria in various foods and good inhibition action on oxidative deterioration of the food. Compared with the existing various preservatives, the compound biological antiseptic and preservation agent has the characteristics of broad antibacterial spectrum, high safety, high efficiency and convenience in use.

The invention provides a cell freezing medium. The cell freezing medium comprises acylated epsilon polylysine, polyhydric alcohols and equilibration buffer. According to the cell freezing medium disclosed by the invention, the polyhydric alcohols and acylated epsilon polylysine replace toxic dimethyl sulfoxide so as to serve as cryoprotective agents, so that damage to cells in the freezing resuscitation process is reduced, and the cell survival rate is improved. The invention further provides a method for performing freezing resuscitation on cells by utilizing the cell freezing medium and application of the method for freezing stem cells and immune cells. The method is simple and feasible to operate and is suitable for large-scale clinical application of cell therapy, and great convenienceis brought to clinical application of the cell freezing technology.

The invention relates to a natural preservative antistaling agent, which comprises epsilon-polylysine and alcohol extractions of anise, black plum, Cinnamomum cassia, and purple perilla, and can improve the cell membrane permeability of microorganism, thereby causing microorganism restricted substance leak, and can inhibit the growth of Gram-positive bacteria and Gram-negative bacterium, fungus, and saccharomycete in fresh-cut vegetables, and forming a layer of protective film on the surface of fruits and vegetables. The invention can prevent the intrusion of microbe, and reduces spoilage. When utilized in fresh-cut vegetables, the preservative antistaling agent can reduces the rotten rate and avoid the shortcoming that general chemical preservative needs lower PH, the invention is particularly suitable to the fresh-keeping of low acid foods such as vegetables, and can reduce loss in weight which is caused by water evaporation, and can be used for fresh-keeping of fresh-cut vegetables, inhibits oxidation and browning reaction and nutrient loss, prolongs the goods shelf life, and is a preservative antistaling agent which is safe and has no side effect. The invention can prolong the shelf life by 2-3 days.

The invention relates to a mutagenized strain of streptomyces albus TUST2, and a method for using the same to produce epsilon-polylysine and a salt of the epsilon-epsilon. The strain is a high-yield mutagenized strain of epsilon-polylysine selected and bred by means of ultraviolet mutagenesis, ultraviolet-chemical mutagenesis, N ion injection mutagenesis, etc from a strain of streptomyces albus TUST2 which is capable of producing epsilon-polylysine and is screened from the earth in Hainan province of China; the mutagenized strain is resistant to 10mg/ml or above S-(2 Aminoethyl )-L-Cysteine and can generate 10 to 30 g/L acid under optimal conditions; zymotic fluid is removed of the streptomyces albus by configuration and is filtered by diatomaceous earth to obtain clear filtrate and to weak acid type ion exchange resin to obtain coarse epsilon-polylysine products which is subjected to ultrafiltration and purification to get epsilon-polylysine and the salt of the epsilon-epsilon. The distribution of molecular weight of epsilon-polylysine is between 4000 and 6500Da.

The invention provides a compound antibacterial agent and a preparation method thereof. The compound antibacterial agent comprises the following components in portions by weight: 100 portions of water, 0.5-10 portions of soybean extract, 0.5-2.5 portions of glycin, 0.005-3.0 portions of epsilon-polylysine, 0.1-5.0 portions of benzalkonium chloride and 0.1-6.0 portions of didecyl dimethyl ammoniumchloride. The preparation method comprises the following steps: diluting the soybean extract with water, and adding the glycin, the epsilon-polylysine, the benzalkonium chloride and the didecyl dimethyl ammonium chloride according to the ratio to obtain the compound antibacterial agent. The compound antibacterial agent is novel antibacterial agent having no harm to people and livestock and high sterilizing capability, can be used in various locations to be sterilized and disinfected, and has the advantages of low cost, wide application range, good sterilizing effect and the like.

The invention relates to the technical field of litchi preservation, and particularly discloses a room-temperature litchi storage preservative, and a preparation method and an application of the preservative. The preservative mainly comprises carboxymethyl-chitosan, nisin, natamycin, tea polyphenol, epsilon-polylysine, citric acid and phytic acid; litchi fruit is soaked in a preservation solution, and then aired, so that the litchi fruit can be stored at a room temperature; the quality and low level browning of the litchi fruit can be kept for a long time at the room temperature. Litchi treated by the preservative has the characteristics that the preservative is good in preservation effect, safe, environment-friendly, simple to operate and convenient to use; the preservative is extensive in source, low in cost, edible, and easy to degrade; no pesticide residue risk exists; short-term preservation during room-temperature storage and room-temperature logistics can be achieved; the preservative is a novel preservation material with a great development potential.

The invention provides a preparation method of natural polyelectrolyte-based core-shell structured nano-fiber. According to the invention, natural products of hyaluronic acid, sodium polymannuronate, heparin, gamma-polyglutamic acid, chitosan, and epsilon-polylysine, and synthesized polymers of polyoxyethylene, polyvinylpyrrolidone, polyethylene glycol, polyvinyl alcohol and polycaprolactone are adopted as raw materials; a soluble composite solution of the materials are prepared; and the natural polyelectrolyte-based core-shell structured nano-fiber is prepared with a high-pressure electrospinning technology. According to the invention, polyelectrolyte and synthesized polymers are separated through the induction of electric fields, such that the core-shell structured nano-fiber is successfully prepared. The nano-fiber prepared by the invention has special potential application in respects such as bioactive compound or medicine packaging, chemical modification, cell stents, medicine releasing, and gene transferring.

The present invention discloses an antibacterial blended regenerated cotton yarn which is characterized by being prepared by the following raw materials by weight: 36-40 parts of waste cotton fabric, 15-17 parts of cotton yarn leftover, 5-8 parts of bamboo fiber, 2-3 parts of graphene oxide, 0.8-1 part of epsilon-polylysine, 9-10 parts of nano-titanium dioxide, 6-8 parts of silica sol, 2-4 parts of waterborne polyurethane, 39-44 parts of polypropylene, 3-5 parts of nano-zinc oxide, 12-14 parts of liquid paraffin, 1.5-2 parts of carboxymethyl cellulose, 1-2 parts of glyceryl stearate and proper amount of water; by mixing of regenerated cotton yarn and modified polypropylene fiber, the characteristics of various fibers can be neutralized, the disadvantages of poor sunscreen property of the cotton yarn and the polypropylene fiber can be made up, the antibacterial property can be enhanced, the antibacterial blended regenerated cotton yarn has high strength, good elasticity, wear resistance, corrosion resistance and thermal resistance, improves product quality, and saves cost.

A process for preparing epsilon-polylysine and its salt from kitasatospora sp. PL6-3 (CCTCC No.M205012) includes screening kitasatospora sp.PL6-3, culturing in the culture medium containing carbon and nitrogen sources, fermenting, centrifugal separation or filtering for removing thallus, and separating epsilon-polylysine and its salt by ion exchange resin method.

The invention relates to a bioactive substance included alginate microcapsule product. The invention is characterized in that: the microcapsule product is a spherical microcapsule with diameter of 100 to 1,000 microns, and the structure of the microcapsule product comprises a microcapsule film and a core, wherein the microcapsule film is a polyelectrolyte composite hydrogel film formed by electrostatic complexing reaction of two macromolecular materials, namely alginate and epsilon-polylysine; and the core is a liquid or hydrogel environment containing a bioactive substance. The microcapsule product is mainly applied to cell transplantation, cell culture and included carriers of bioactive substances such as protein, polypeptide, enzyme, nucleic acid and the like.

The invention discloses a pork fresh-keeping agent with an excellent fresh-keeping performance. The pork fresh-keeping agent is characterized by comprising the following materials in parts by weight: 20-40 parts of clove and cinnamon compound extracting solution, 5-15 parts of pomegranate extracting solution, 2-5 parts of chitosan, 3-6 parts of trehalose, 2-6 parts of propolis, 3-8 parts of lysozyme, 1-4 parts of tea polyphenol, 2-5 parts of Epsilon-polylysine, 1-4 parts of acetic acid, 1-3 parts of sodium lactate, 1-3 parts of grape-seed essential oil, 1-3 parts of orange essential oil and 2-4 parts of bacillus natto. The invention also discloses a preparation method of the pork fresh-keeping agent with the excellent fresh-keeping performance. The pork fresh-keeping agent disclosed by the invention has excellent preservative and fresh-keeping performances.

The invention discloses an endotoxin adsorbent using a molecular cluster as a functional group and a preparation method thereof, and belongs to the fields of bioseparation and biomedical engineering. By taking agarose gel as a vector, the endotoxin adsorbent is subjected to epoxy activation to be directly coupled with a molecular sieve frame epsilon-polylysine or polyaspartic acid, then the coupled mixture is ingrafted with micromolecular lysine or lycine by virtue of a condensation reaction to prepare the adsorbent. The adsorbent prepared by the method has higher removal rate to endotoxin inserum of a human, but adsorbs less serum protein. Meanwhile, the preparation process of the adsorbent has the advantages of mild conditions, fewer synthesis steps and relatively low material cost.

The invention relates to an epsilon-polylysine bionic antibacterial membrane and a preparation method and application thereof. The method for preparing the epsilon-polylysine bionic antibacterial membrane is characterized by comprising the following steps: preparing a gelatinous aqueous solution containing epsilon-polylysine, chitosan, hyaluronic acid, gelatin, polyvinyl alcohol and glycerol, thenadding a cross-linking agent to perform a cross-linking reaction in a patterned template to obtain a gel, and then neutralizing, washing, and drying. The epsilon-polylysine bionic antibacterial membrane has good antibacterial and moisturizing properties, and the composition approximates an extracellular matrix and has a patterned surface that can promote epithelial cell adhesion, growth and migration. Therefore, the epsilon-polylysine bionic antibacterial membrane can be used as a skin wound dressing to improve the wound healing effect.

The invention provides a biological microcapsule, in particular relating to a preparation method of an alginate/epsilon-polylysine/alginate biological microcapsule (epsilon-APA microcapsule). Based on the characteristic that the highly polymerized multivalence-cationic epsilon-polylysine has a high electrostatic interaction with a substance with negative ions, the epsilon-polylysine is taken as amicrocapsule film material, and the alginate microcapsule is externally made into a coating film with slow-release performance to change the permeability and immune isolation of the biological microcapsule, so that the epsilon-APA microcapsule is used for the biological and medical fields such as the cell transplantation, the cell culture, the drug release, and the like.

The invention relates to the technical field of medical gels, and in particular relates to a recombinant human collagen gel and a preparation method thereof. The recombinant human collagen gel consists of the following raw materials in percentage by weight: 1-3% of small-molecule collagen, 1.5-2.5% of glycerol, 1-2% of chitosan quaternary ammonium salt, 0.5-1.5% of sodium hyaluronate, 0.5-1.5% of epsilon-polylysine, 0.3-0.7% of borneol, 0.3-0.7% of poloxamer, 0.3-0.7% of coenzyme Q10, 0.3-0.7% of butanediol and the balance of purified water. The recombinant human collagen gel provided by the invention has effects of moisture retention and isolation, can improve vaginal atrophy, increase vaginal elasticity and relieve dyspareunia, and also can improve the wettability and the dry state of a vagina, relieve dry and coarse stabbing pain symptoms of cunnus, and ease itching, calor, skin mucous membrane fissure and diffuse superficial bleeding caused by vaginal dryness.

The invention discloses a dressing preparation method of an antibacterial amorphous hydrogel based on biological properties, which comprises the following steps: step 1, weighing water, adding sodium carboxymethyl cellulose to the water slowly at normal temperature, stirring, completely dissolving the sodium carboxymethyl cellulose, and putting aside to prepare a component A; step 2, weighing water, adding sodium alginate to the water at normal temperature, quickly stirring, completely dissolving the sodium alginate, and putting aside for future use to prepare a component B, step 3, weighing water, adding Saxifraga dshagalensis rubber to the water at normal temperature, stirring at a high shearing speed, completely dissolving the Saxifraga dshagalensis rubber, and putting aside for future use to prepare a component C; step 4, weighing water, adding epsilon-polylysine to the water, slowly stirring, and putting aside for future use to prepare a component D; and step 5, mixing the component A, the component B and the component C together, stirring at the high shearing speed, slowly stirring so that the components A, B and C are dissolved mutually, and slowly adding the component D, adequately dissolving the component D to form a gel, sterilizing and packaging in a bottle for use.

The invention discloses a freshness keeping method for preventing darkening of southern American white prawns, which specifically comprises the steps of: (1) preparing fixed amount of biological freshness keeping agent solution; (2) collecting and transporting healthy southern American white prawns with the uniform size while the southern American white prawns are kept alive; (3) picking out abnormal individuals such as dead, mutilation, color change and the like; (4) washing the selected prawn bodies with sterilized water, and putting the prawn bodies in crushed ice to shock the prawn bodies; (5) soaking the well-treated southern American white prawns in the freshness keeping agent solution; and (6) putting the southern American white prawns in a refrigerator at 3 DEG C for the purpose of storage. The freshness keeping agent solution consists of phytic acid, chitosan, epsilon-polylysine, glacial acetic acid and water, with the proportions thereof as follows: 0.05% to 0.1% of the phytic acid, 1% to 2% of the chitosan, 0.05% to 0.15% of epsilon-polylysine, 0.8% to 1.2% of the glacial acetic acid and the balance of water. The freshness keeping agent solution prepared by adopting themethod disclosed by the invention can favorably inhibit darkening and bacteria-based spoilage of the southern American white prawns, and compared with the way of refrigerated storage at 3 DEG C, the freshness keeping agent can remarkably prolong the shelf life of the southern American white prawns by 5 to 6 days.

The invention relates to biological antibiotic cotton fibers or cotton cloth and a method for preparing the same. In the preparation method, a biological preservative of epsilon-polylysine serving as a bacteriostatic agent is subjected to grafting reaction with cotton fibers or cotton cloth undergoing oxidation treatment by sodium periodate or subjected to adsorption combination with cotton fibers or cotton cloth not undergoing oxidation treatment to form the cotton fibers or cotton cloth having antibiotic and bacteriostatic effects. Because the anti-bacterial agent used in the cotton fibers or cotton cloth is the bacteriostatic agent of epsilon-polylysine, the biological antibiotic cotton fibers or cotton cloth is high in safety. The biological antibiotic cotton fibers or cotton cloth can be used in production of garments and hygienic products.

The invention relates to a photodynamic antibacterial cellulose material and a preparation method thereof. Epsilon-polylysine is attached to the cellulose material and further modified with photo-sensitizers through amidogen of the epsilon-polylysine, the epsilon-polylysine provides a first antibacterial performance of the material, and the photo-sensitizers provide a second antibacterial performance of the material. The preparation method of the material is simple in process, mild in reaction condition and suitable for industrial production, and final cellulose products with double antibacterial performances can be used for preparing medical antibacterial fibers or daily antibacterial textiles.

The invention discloses a streptomyces sp. NK-49 and an application thereof; and the application is a method for fermenting, cultivating, separating and purifying Epsilon-polylysine. The method comprises the following steps: firstly, picking a ring of spores from streptomyces sp. NK-49 which is stored on a Beinart inclined plane, and inoculating the spores serving as seeds in a Gao 1th culture medium for activated culture within 20 to 36 h; inoculating 5 to 15 percent of seed liquid to a synthetic medium taking amylolysis sugar or amylolysis sugar and glycerol as a carbon source, and shaking a flask for fermentation and culture for 48 to 96 h; after fermentation, conducting 6000 to 10000 rpm centrifugation on the fermentation liquid to remove thallus, collecting supernate, regulating the pH of the supernate to 8 by utilizing 5M NaOH solution, and filtering to removing deposited hetero proteins; utilizing weak acid type cation exchange resin to adsorb and remove Epsilon-polylysine molecules from the supernate of the hetero proteins, and washing with distilled water; eluting the adsorbed products by utilizing 0.1 to 0.7 mol/L dilute hydrochloric acid, and utilizing 5M NaOH solution to regulate the pH of eluant to a neutral value; and dialyzing the neutral eluant to remove micro molecular impurities, freezing, and drying to obtain Epsilon-polylysine solid powder. The streptomyces sp. NK-49 obtained through separation has the potential for industrial production of Epsilon-polylysine.

The invention relates to an epsilon-polylysine high-yielding strain and an epsilon-polylysine production method, and belongs to the biotechnical field. The epsilon-polylysine high-yielding strain EA-19 is named as Streptomyces albus, and the preservation number is CGMCC No.10156. The strain EA-19 is used to carry out fed batch fermentation culture, and the concentration of epsilon-polylysine in a fermentation liquid reaches 38.5-39 g/L. The epsilon-polylysine production method comprises is characterized in that the strain EA-19 is adopted to carry out fermentation culture. A bubbling technology is adopted, ventilation is carried out in and after the fermentation culture process, so epsilon-polylysine generated after fermentation exists in foams, and is separated from the fermentation liquid, so the technical problem of difficulty separation of epsilon-polylysine in present preparation methods is solved, a step of ion exchange which causes generation of a large amount of wastewater is omitted, and the product yield is improved by 50% or above. The method has the advantages of simple process, easiness in amplification, low energy consumption, small device investment, and easiness in realization of large-scale production.

The invention relates to a sterilization laundry detergent containing epsilon-polylysine. The sterilization laundry detergent comprises the following raw materials in percentage by weight: 10-50% of surfactant, 0-2% of anti-redeposition agent, 0.5-1% of chelating agent, 0-3% of thickening agent, 0.1-1% of epsilon-polylysine, 0.1-0.5% of essence and the balance of deionized water. According to the sterilization laundry detergent provided by the invention, by taking the epsilon-polylysine as a sterilization agent, the antibacterial sterilization effect is obvious, the safety is high, and the need of consumers for natural products is met.