401 results about "Epsilon-Polylysine" patented technology

It has been desired to develop a highly preservative and antibacterial cosmetic composition that can easily be applied to both emulsion and non-emulsion type cosmetics. It has also been desired to develop a method of improving a preservative and / or antibacterial effect(s) of a cosmetic composition comprising polyorganosiloxane-containing epsilon-polylysine and thereby reducing the amount of antibacterial preservative agent to be used. There is provided a cosmetic composition comprising one or a combination of two or more of polyorganosiloxane-containing epsilon-polylysine compounds obtained by reacting epsilon-polylysine with polyorganosiloxane or a physiologically acceptable salt thereof, and polyhydric alcohol.

The invention relates to a compound biological antiseptic and preservation agent for food. The compound biological antiseptic and preservation agent is prepared by evenly mixing pure biological solid preparations or products such as epsilon-polylysine, nisin, natamycin, lysozyme, bacillus subtilis, ascorbic acid, a toona sinensis leaf extract, a water-soluble resveratrol preparation, tea polyphenol and chitosan. The compound biological antiseptic and preservation agent has a strong inhibition effect on spoilage bacteria and pathogenic bacteria in various foods and good inhibition action on oxidative deterioration of the food. Compared with the existing various preservatives, the compound biological antiseptic and preservation agent has the characteristics of broad antibacterial spectrum, high safety, high efficiency and convenience in use.

The invention provides a cell freezing medium. The cell freezing medium comprises acylated epsilon polylysine, polyhydric alcohols and equilibration buffer. According to the cell freezing medium disclosed by the invention, the polyhydric alcohols and acylated epsilon polylysine replace toxic dimethyl sulfoxide so as to serve as cryoprotective agents, so that damage to cells in the freezing resuscitation process is reduced, and the cell survival rate is improved. The invention further provides a method for performing freezing resuscitation on cells by utilizing the cell freezing medium and application of the method for freezing stem cells and immune cells. The method is simple and feasible to operate and is suitable for large-scale clinical application of cell therapy, and great convenienceis brought to clinical application of the cell freezing technology.

The invention relates to a natural preservative antistaling agent, which comprises epsilon-polylysine and alcohol extractions of anise, black plum, Cinnamomum cassia, and purple perilla, and can improve the cell membrane permeability of microorganism, thereby causing microorganism restricted substance leak, and can inhibit the growth of Gram-positive bacteria and Gram-negative bacterium, fungus, and saccharomycete in fresh-cut vegetables, and forming a layer of protective film on the surface of fruits and vegetables. The invention can prevent the intrusion of microbe, and reduces spoilage. When utilized in fresh-cut vegetables, the preservative antistaling agent can reduces the rotten rate and avoid the shortcoming that general chemical preservative needs lower PH, the invention is particularly suitable to the fresh-keeping of low acid foods such as vegetables, and can reduce loss in weight which is caused by water evaporation, and can be used for fresh-keeping of fresh-cut vegetables, inhibits oxidation and browning reaction and nutrient loss, prolongs the goods shelf life, and is a preservative antistaling agent which is safe and has no side effect. The invention can prolong the shelf life by 2-3 days.

The invention relates to a mutagenized strain of streptomyces albus TUST2, and a method for using the same to produce epsilon-polylysine and a salt of the epsilon-epsilon. The strain is a high-yield mutagenized strain of epsilon-polylysine selected and bred by means of ultraviolet mutagenesis, ultraviolet-chemical mutagenesis, N ion injection mutagenesis, etc from a strain of streptomyces albus TUST2 which is capable of producing epsilon-polylysine and is screened from the earth in Hainan province of China; the mutagenized strain is resistant to 10mg / ml or above S-(2 Aminoethyl )-L-Cysteine and can generate 10 to 30 g / L acid under optimal conditions; zymotic fluid is removed of the streptomyces albus by configuration and is filtered by diatomaceous earth to obtain clear filtrate and to weak acid type ion exchange resin to obtain coarse epsilon-polylysine products which is subjected to ultrafiltration and purification to get epsilon-polylysine and the salt of the epsilon-epsilon. The distribution of molecular weight of epsilon-polylysine is between 4000 and 6500Da.

The invention provides a compound antibacterial agent and a preparation method thereof. The compound antibacterial agent comprises the following components in portions by weight: 100 portions of water, 0.5-10 portions of soybean extract, 0.5-2.5 portions of glycin, 0.005-3.0 portions of epsilon-polylysine, 0.1-5.0 portions of benzalkonium chloride and 0.1-6.0 portions of didecyl dimethyl ammoniumchloride. The preparation method comprises the following steps: diluting the soybean extract with water, and adding the glycin, the epsilon-polylysine, the benzalkonium chloride and the didecyl dimethyl ammonium chloride according to the ratio to obtain the compound antibacterial agent. The compound antibacterial agent is novel antibacterial agent having no harm to people and livestock and high sterilizing capability, can be used in various locations to be sterilized and disinfected, and has the advantages of low cost, wide application range, good sterilizing effect and the like.

The invention relates to the technical field of litchi preservation, and particularly discloses a room-temperature litchi storage preservative, and a preparation method and an application of the preservative. The preservative mainly comprises carboxymethyl-chitosan, nisin, natamycin, tea polyphenol, epsilon-polylysine, citric acid and phytic acid; litchi fruit is soaked in a preservation solution, and then aired, so that the litchi fruit can be stored at a room temperature; the quality and low level browning of the litchi fruit can be kept for a long time at the room temperature. Litchi treated by the preservative has the characteristics that the preservative is good in preservation effect, safe, environment-friendly, simple to operate and convenient to use; the preservative is extensive in source, low in cost, edible, and easy to degrade; no pesticide residue risk exists; short-term preservation during room-temperature storage and room-temperature logistics can be achieved; the preservative is a novel preservation material with a great development potential.

The invention discloses an antibacterial self-healing hydrogel dressing capable of accelerating healing of chronic wounds as well as a preparation method and application thereof. The method comprisesthe following steps: firstly oxidizing sodium hyaluronate, sodium alginate and pulullan through a strong oxidant to obtain polysaccharide with aldehyde group, then forming a polymer with network withcationic polymers such as epsilon-polylysine, polyethyleneimine and chitosan through Schiff base reaction, then fully mixing the polymer with F127 to form a hydrogel with a dual-network structure, finally loading exosome on the cationic polymers through electrostatic effect, coating the exosome in the hydrogel with the dual-network structure to obtain the antibacterial self-healing hydrogel dressing capable of accelerating healing of the chronic wounds. The preparation method is simple and is free of organic solvent residues; the used chemical synthesis method is environmentally friendly and is convenient to operate and low in raw material cost.

The invention provides a preparation method of natural polyelectrolyte-based core-shell structured nano-fiber. According to the invention, natural products of hyaluronic acid, sodium polymannuronate, heparin, gamma-polyglutamic acid, chitosan, and epsilon-polylysine, and synthesized polymers of polyoxyethylene, polyvinylpyrrolidone, polyethylene glycol, polyvinyl alcohol and polycaprolactone are adopted as raw materials; a soluble composite solution of the materials are prepared; and the natural polyelectrolyte-based core-shell structured nano-fiber is prepared with a high-pressure electrospinning technology. According to the invention, polyelectrolyte and synthesized polymers are separated through the induction of electric fields, such that the core-shell structured nano-fiber is successfully prepared. The nano-fiber prepared by the invention has special potential application in respects such as bioactive compound or medicine packaging, chemical modification, cell stents, medicine releasing, and gene transferring.

The invention provides a method for extracting epsilon-polylysine and salt thereof from fermentation liquid, which comprises the following steps: in an acidification tank, the fermentation liquid is acidized and then filtrated to obtain clear filtrate; and then the clean filtrate is alkalized, made into alkalified solution and then pressed into a resin adsorption column to be absorbed; firstly saturated resin is washed with purified water and then analyzed with hydrochloric acid to obtain analytic liquid which is decolorized and filtrated by activated carbon to obtain destaining solution; the destaining solution is pressed into a membrane filtration device to be circularly concentrated so as to obtain concentrated solution; the concentrated solution is sprayed or decompressed, frozen and dried to obtain the epsilon-polylysine pure product. The raw material and the instrument adopted in the method are easily obtained and have low cost; the extraction and purification technique is simple and practicable; and the final product has high yield and high purity. The prepared epsilon-polylysine can be used as preservative, biological high polymer material, micro-capsule technique material, food therapy agent, drug carriers, biological chips, high polymer nonabsorbent material and the like.

The present invention discloses an antibacterial blended regenerated cotton yarn which is characterized by being prepared by the following raw materials by weight: 36-40 parts of waste cotton fabric, 15-17 parts of cotton yarn leftover, 5-8 parts of bamboo fiber, 2-3 parts of graphene oxide, 0.8-1 part of epsilon-polylysine, 9-10 parts of nano-titanium dioxide, 6-8 parts of silica sol, 2-4 parts of waterborne polyurethane, 39-44 parts of polypropylene, 3-5 parts of nano-zinc oxide, 12-14 parts of liquid paraffin, 1.5-2 parts of carboxymethyl cellulose, 1-2 parts of glyceryl stearate and proper amount of water; by mixing of regenerated cotton yarn and modified polypropylene fiber, the characteristics of various fibers can be neutralized, the disadvantages of poor sunscreen property of the cotton yarn and the polypropylene fiber can be made up, the antibacterial property can be enhanced, the antibacterial blended regenerated cotton yarn has high strength, good elasticity, wear resistance, corrosion resistance and thermal resistance, improves product quality, and saves cost.

The invention relates to a bacteriostat composition applicable to plant tissue culture. The bacteriostat composition comprises the following components in percentage by volume: 2.5 percent of epsilon-polylysine, 22 to 29.5 percent of isothiazolinone, 10 to 15 percent of traditional Chinese medicine water extracts, 2.5 percent of potassium sorbate, sodium benzoate and the like, 3.5 percent of stabilizing agent and 47.0 to 59.5 percent of deionized water. When the composition is used for disinfecting explants of plants, the explants are subjected to routine disinfection after being soaked in the bacteriostat, so that the treatment time on corrosive sublimate or sodium hypochlorite can be reduced and the aims of sterilizing and keeping relatively high survival rate of the explants are fulfilled; the bacteriostat is added into a first generation culture medium or a subculture medium, so pollution of fungus and bacteria can be effectively reduced and prevented and the bacteriostat does not have adverse effect on the plant growth; and when applied to plant open culture, such as a strong seeding and rooting stage and the like, the bacteriostat composition can save the culture medium high-pressure sterilizing process, does not need a clean bench, can save energy and reduce the cost of tissue culture seedlings, and improves benefits.

A process for preparing epsilon-polylysine and its salt from kitasatospora sp. PL6-3 (CCTCC No.M205012) includes screening kitasatospora sp.PL6-3, culturing in the culture medium containing carbon and nitrogen sources, fermenting, centrifugal separation or filtering for removing thallus, and separating epsilon-polylysine and its salt by ion exchange resin method.

The invention relates to a bioactive substance included alginate microcapsule product. The invention is characterized in that: the microcapsule product is a spherical microcapsule with diameter of 100 to 1,000 microns, and the structure of the microcapsule product comprises a microcapsule film and a core, wherein the microcapsule film is a polyelectrolyte composite hydrogel film formed by electrostatic complexing reaction of two macromolecular materials, namely alginate and epsilon-polylysine; and the core is a liquid or hydrogel environment containing a bioactive substance. The microcapsule product is mainly applied to cell transplantation, cell culture and included carriers of bioactive substances such as protein, polypeptide, enzyme, nucleic acid and the like.

The invention discloses a pork fresh-keeping agent with an excellent fresh-keeping performance. The pork fresh-keeping agent is characterized by comprising the following materials in parts by weight: 20-40 parts of clove and cinnamon compound extracting solution, 5-15 parts of pomegranate extracting solution, 2-5 parts of chitosan, 3-6 parts of trehalose, 2-6 parts of propolis, 3-8 parts of lysozyme, 1-4 parts of tea polyphenol, 2-5 parts of Epsilon-polylysine, 1-4 parts of acetic acid, 1-3 parts of sodium lactate, 1-3 parts of grape-seed essential oil, 1-3 parts of orange essential oil and 2-4 parts of bacillus natto. The invention also discloses a preparation method of the pork fresh-keeping agent with the excellent fresh-keeping performance. The pork fresh-keeping agent disclosed by the invention has excellent preservative and fresh-keeping performances.

The invention discloses an endotoxin adsorbent using a molecular cluster as a functional group and a preparation method thereof, and belongs to the fields of bioseparation and biomedical engineering. By taking agarose gel as a vector, the endotoxin adsorbent is subjected to epoxy activation to be directly coupled with a molecular sieve frame epsilon-polylysine or polyaspartic acid, then the coupled mixture is ingrafted with micromolecular lysine or lycine by virtue of a condensation reaction to prepare the adsorbent. The adsorbent prepared by the method has higher removal rate to endotoxin inserum of a human, but adsorbs less serum protein. Meanwhile, the preparation process of the adsorbent has the advantages of mild conditions, fewer synthesis steps and relatively low material cost.

The invention relates to an epsilon-polylysine bionic antibacterial membrane and a preparation method and application thereof. The method for preparing the epsilon-polylysine bionic antibacterial membrane is characterized by comprising the following steps: preparing a gelatinous aqueous solution containing epsilon-polylysine, chitosan, hyaluronic acid, gelatin, polyvinyl alcohol and glycerol, thenadding a cross-linking agent to perform a cross-linking reaction in a patterned template to obtain a gel, and then neutralizing, washing, and drying. The epsilon-polylysine bionic antibacterial membrane has good antibacterial and moisturizing properties, and the composition approximates an extracellular matrix and has a patterned surface that can promote epithelial cell adhesion, growth and migration. Therefore, the epsilon-polylysine bionic antibacterial membrane can be used as a skin wound dressing to improve the wound healing effect.

The invention discloses foliar fertilizer. The foliar fertilizer comprises boric acid, potassium nitrate, epsilon-polylysine, polyacrylamide, ammonium molybdate, butanedioic acid, trace elements, sodium nitrophenolate, water and chelating agent. The foliar fertilizer contains multiple trace elements such as zinc, boron, copper, iron, manganese and molybdenum which are necessary for crops. By adopting the foliar fertilizer, the application amount of pesticides can be reduced, the cost is reduced, the development of the crops is promoted, and the utilization rate of photosynthesis is increased. Experiments prove that the foliar fertilizer is capable of promoting the adsorption of carbon, hydrogen and oxygen and preventing and treating diseases and insect pests.

The invention provides a biological antibacterial composition and application of the biological antibacterial composition in cosmetics. The biological antibacterial composition comprises the following components in parts by weight: 0.25 to 1.0 part of epsilon-polylysine, 0.1 to 0.6 parts of nisin, 0.05 to 0.4 parts of natamycin, 3 to 8 parts of glycerin, 2 to 8 parts of 1,3-butanediol and 0.2 to 2 parts of caprylyl glycol. The composition provided by the invention has good antibacterial effect, particularly has a significant synergistic effect on inhibiting candida albicans, pseudomonas aeruginosa and aspergillus niger, can be used in skin care and washing cosmetics, and the additive amount of the original preservatives is reduced.

The invention discloses a foliar fertilizer. The foliar fertilizer comprises boric acid, potassium carbonate, epsilon-polylysine, humic acid, itaconic acid, trace elements, diethyl aminoethyl hexanoate, water and a chelating agent The foliar fertilizer has the advantages of disease prevention, bacteriostasis, adversity resistance, hazard elimination, richness in boron and potassium, satisfying of the crop growth requirements, and economic benefit enhancement.

The invention provides a biological microcapsule, in particular relating to a preparation method of an alginate / epsilon-polylysine / alginate biological microcapsule (epsilon-APA microcapsule). Based on the characteristic that the highly polymerized multivalence-cationic epsilon-polylysine has a high electrostatic interaction with a substance with negative ions, the epsilon-polylysine is taken as amicrocapsule film material, and the alginate microcapsule is externally made into a coating film with slow-release performance to change the permeability and immune isolation of the biological microcapsule, so that the epsilon-APA microcapsule is used for the biological and medical fields such as the cell transplantation, the cell culture, the drug release, and the like.

The invention relates to the technical field of medical gels, and in particular relates to a recombinant human collagen gel and a preparation method thereof. The recombinant human collagen gel consists of the following raw materials in percentage by weight: 1-3% of small-molecule collagen, 1.5-2.5% of glycerol, 1-2% of chitosan quaternary ammonium salt, 0.5-1.5% of sodium hyaluronate, 0.5-1.5% of epsilon-polylysine, 0.3-0.7% of borneol, 0.3-0.7% of poloxamer, 0.3-0.7% of coenzyme Q10, 0.3-0.7% of butanediol and the balance of purified water. The recombinant human collagen gel provided by the invention has effects of moisture retention and isolation, can improve vaginal atrophy, increase vaginal elasticity and relieve dyspareunia, and also can improve the wettability and the dry state of a vagina, relieve dry and coarse stabbing pain symptoms of cunnus, and ease itching, calor, skin mucous membrane fissure and diffuse superficial bleeding caused by vaginal dryness.

The invention discloses a dressing preparation method of an antibacterial amorphous hydrogel based on biological properties, which comprises the following steps: step 1, weighing water, adding sodium carboxymethyl cellulose to the water slowly at normal temperature, stirring, completely dissolving the sodium carboxymethyl cellulose, and putting aside to prepare a component A; step 2, weighing water, adding sodium alginate to the water at normal temperature, quickly stirring, completely dissolving the sodium alginate, and putting aside for future use to prepare a component B, step 3, weighing water, adding Saxifraga dshagalensis rubber to the water at normal temperature, stirring at a high shearing speed, completely dissolving the Saxifraga dshagalensis rubber, and putting aside for future use to prepare a component C; step 4, weighing water, adding epsilon-polylysine to the water, slowly stirring, and putting aside for future use to prepare a component D; and step 5, mixing the component A, the component B and the component C together, stirring at the high shearing speed, slowly stirring so that the components A, B and C are dissolved mutually, and slowly adding the component D, adequately dissolving the component D to form a gel, sterilizing and packaging in a bottle for use.

The invention discloses a freshness keeping method for preventing darkening of southern American white prawns, which specifically comprises the steps of: (1) preparing fixed amount of biological freshness keeping agent solution; (2) collecting and transporting healthy southern American white prawns with the uniform size while the southern American white prawns are kept alive; (3) picking out abnormal individuals such as dead, mutilation, color change and the like; (4) washing the selected prawn bodies with sterilized water, and putting the prawn bodies in crushed ice to shock the prawn bodies; (5) soaking the well-treated southern American white prawns in the freshness keeping agent solution; and (6) putting the southern American white prawns in a refrigerator at 3 DEG C for the purpose of storage. The freshness keeping agent solution consists of phytic acid, chitosan, epsilon-polylysine, glacial acetic acid and water, with the proportions thereof as follows: 0.05% to 0.1% of the phytic acid, 1% to 2% of the chitosan, 0.05% to 0.15% of epsilon-polylysine, 0.8% to 1.2% of the glacial acetic acid and the balance of water. The freshness keeping agent solution prepared by adopting themethod disclosed by the invention can favorably inhibit darkening and bacteria-based spoilage of the southern American white prawns, and compared with the way of refrigerated storage at 3 DEG C, the freshness keeping agent can remarkably prolong the shelf life of the southern American white prawns by 5 to 6 days.

The invention relates to a production method of an epsilon-polylysine and polyvinyl alcohol composite biological antibacterial film. One or two components selected from glycerol and ethylene glycol are taken as a compound plasticizer and the epsilon-polylysine and polyvinyl alcohol composite polymer film is prepared by a melt blending method. The addition of epsilon-polylysine endows the film with good antibacterial activity and the film can be used as a packaging material and an agricultural film.

The invention relates to biological antibiotic cotton fibers or cotton cloth and a method for preparing the same. In the preparation method, a biological preservative of epsilon-polylysine serving as a bacteriostatic agent is subjected to grafting reaction with cotton fibers or cotton cloth undergoing oxidation treatment by sodium periodate or subjected to adsorption combination with cotton fibers or cotton cloth not undergoing oxidation treatment to form the cotton fibers or cotton cloth having antibiotic and bacteriostatic effects. Because the anti-bacterial agent used in the cotton fibers or cotton cloth is the bacteriostatic agent of epsilon-polylysine, the biological antibiotic cotton fibers or cotton cloth is high in safety. The biological antibiotic cotton fibers or cotton cloth can be used in production of garments and hygienic products.

The invention relates to a photodynamic antibacterial cellulose material and a preparation method thereof. Epsilon-polylysine is attached to the cellulose material and further modified with photo-sensitizers through amidogen of the epsilon-polylysine, the epsilon-polylysine provides a first antibacterial performance of the material, and the photo-sensitizers provide a second antibacterial performance of the material. The preparation method of the material is simple in process, mild in reaction condition and suitable for industrial production, and final cellulose products with double antibacterial performances can be used for preparing medical antibacterial fibers or daily antibacterial textiles.

The invention discloses a streptomyces sp. NK-49 and an application thereof; and the application is a method for fermenting, cultivating, separating and purifying Epsilon-polylysine. The method comprises the following steps: firstly, picking a ring of spores from streptomyces sp. NK-49 which is stored on a Beinart inclined plane, and inoculating the spores serving as seeds in a Gao 1th culture medium for activated culture within 20 to 36 h; inoculating 5 to 15 percent of seed liquid to a synthetic medium taking amylolysis sugar or amylolysis sugar and glycerol as a carbon source, and shaking a flask for fermentation and culture for 48 to 96 h; after fermentation, conducting 6000 to 10000 rpm centrifugation on the fermentation liquid to remove thallus, collecting supernate, regulating the pH of the supernate to 8 by utilizing 5M NaOH solution, and filtering to removing deposited hetero proteins; utilizing weak acid type cation exchange resin to adsorb and remove Epsilon-polylysine molecules from the supernate of the hetero proteins, and washing with distilled water; eluting the adsorbed products by utilizing 0.1 to 0.7 mol / L dilute hydrochloric acid, and utilizing 5M NaOH solution to regulate the pH of eluant to a neutral value; and dialyzing the neutral eluant to remove micro molecular impurities, freezing, and drying to obtain Epsilon-polylysine solid powder. The streptomyces sp. NK-49 obtained through separation has the potential for industrial production of Epsilon-polylysine.

The invention relates to an epsilon-polylysine high-yielding strain and an epsilon-polylysine production method, and belongs to the biotechnical field. The epsilon-polylysine high-yielding strain EA-19 is named as Streptomyces albus, and the preservation number is CGMCC No.10156. The strain EA-19 is used to carry out fed batch fermentation culture, and the concentration of epsilon-polylysine in a fermentation liquid reaches 38.5-39 g / L. The epsilon-polylysine production method comprises is characterized in that the strain EA-19 is adopted to carry out fermentation culture. A bubbling technology is adopted, ventilation is carried out in and after the fermentation culture process, so epsilon-polylysine generated after fermentation exists in foams, and is separated from the fermentation liquid, so the technical problem of difficulty separation of epsilon-polylysine in present preparation methods is solved, a step of ion exchange which causes generation of a large amount of wastewater is omitted, and the product yield is improved by 50% or above. The method has the advantages of simple process, easiness in amplification, low energy consumption, small device investment, and easiness in realization of large-scale production.

The invention relates to a sterilization laundry detergent containing epsilon-polylysine. The sterilization laundry detergent comprises the following raw materials in percentage by weight: 10-50% of surfactant, 0-2% of anti-redeposition agent, 0.5-1% of chelating agent, 0-3% of thickening agent, 0.1-1% of epsilon-polylysine, 0.1-0.5% of essence and the balance of deionized water. According to the sterilization laundry detergent provided by the invention, by taking the epsilon-polylysine as a sterilization agent, the antibacterial sterilization effect is obvious, the safety is high, and the need of consumers for natural products is met.