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Epsilon-polylysine bionic antibacterial membrane, preparation and application thereof

A technology of polylysine and chitosan, which is applied in the field of regenerative medicine, can solve the problems of limited antibacterial effect, inability to adhere to growth, migration and fusion of skin epithelial cells, achieve good affinity, promote wound healing, The effect of improving the antibacterial ability

Active Publication Date: 2018-05-25
大连敏慧精益科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current chitosan-based wound dressings still have two serious defects: 1) chitosan widely exists in nature, and the flora has a tolerance to it, so the antibacterial effect of the existing dressings is limited; 2) the dressing composition It is very different from the interface morphology and extracellular matrix, and cannot effectively guide the adhesion, growth, migration and fusion of skin epithelial cells around the wound
However, although ε-polylysine has good antibacterial properties, it has not been applied to skin surface wound dressings

Method used

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  • Epsilon-polylysine bionic antibacterial membrane, preparation and application thereof

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Experimental program
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Effect test

Embodiment 1

[0030] Preparation containing 10% (w / v, g / ml) ε-polylysine (molecular weight is 3000kDa), 1% (w / v, g / ml) chitosan (molecular weight is 300000kDa), 1% (w / v, g / ml) hyaluronic acid (1000000kDa), 15% (w / v, g / ml) acid gelatin (jelly strength 150Bloom g), 1% (w / v, g / ml) polyvinyl alcohol ( Polymerization degree is 20,000), 10% (v / v) glycerin gel-forming aqueous solution. Afterwards, add cross-linking agent glutaraldehyde in gel-forming aqueous solution, the final concentration of glutaraldehyde is 3% (w / v, g / ml), and patterned template ( figure 1 is the bottom surface pattern, the groove width is 40 microns, and the groove depth is 60 microns) for cross-linking reaction to obtain a gel. The cross-linking conditions were a temperature of 23° C. and a humidity of 80%. Afterwards, the cross-linked gel was soaked with 0.1M glycine solution, neutralized, and washed with water. Then, it was dried in an environment with a temperature of 20° C. and a humidity of 20%, to obtain an ε-poly...

Embodiment 2

[0034] Preparation containing 1% (w / v, g / ml) ε-polylysine (molecular weight is 6000kDa), 10% (w / v, g / ml) chitosan (molecular weight is 5000kDa), 0.1% (w / v, g / ml) hyaluronic acid (4000000kDa), 3% (w / v, g / ml) alkaline gelatin (jelly strength 220Bloom g), 5% (w / v, g / ml) polyvinyl alcohol (The degree of polymerization is 50,000), 1% (v / v) glycerin gel-forming aqueous solution. Afterwards, add cross-linking agent glutaraldehyde in the gel-forming aqueous solution, the final concentration of glutaraldehyde is 0.01% (w / v, g / ml), and patterned template ( figure 1 , carry out cross-linking reaction with embodiment 1), obtain gel. The cross-linking conditions were a temperature of 25° C. and a humidity of 50%. Afterwards, the cross-linked gel was soaked with 0.1M glycine solution, neutralized, and washed with water. Then, it was dried in an environment with a temperature of 22° C. and a humidity of 20%, to obtain an ε-polylysine biomimetic bacteriostatic film. Set up 4 control grou...

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Abstract

The invention relates to an epsilon-polylysine bionic antibacterial membrane and a preparation method and application thereof. The method for preparing the epsilon-polylysine bionic antibacterial membrane is characterized by comprising the following steps: preparing a gelatinous aqueous solution containing epsilon-polylysine, chitosan, hyaluronic acid, gelatin, polyvinyl alcohol and glycerol, thenadding a cross-linking agent to perform a cross-linking reaction in a patterned template to obtain a gel, and then neutralizing, washing, and drying. The epsilon-polylysine bionic antibacterial membrane has good antibacterial and moisturizing properties, and the composition approximates an extracellular matrix and has a patterned surface that can promote epithelial cell adhesion, growth and migration. Therefore, the epsilon-polylysine bionic antibacterial membrane can be used as a skin wound dressing to improve the wound healing effect.

Description

technical field [0001] The invention relates to the field of regenerative medicine, in particular to an ε-polylysine biomimetic bacteriostatic film and its preparation and application. Background technique [0002] Skin is the tissue on the surface of the body that covers the muscles and is the largest organ of the human body. It covers the whole body and protects various tissues and organs in the body from physical, mechanical, chemical and pathogenic microorganisms. Burns, scalds, and wounds are common skin injuries. These injuries cause the loss of the local barrier function of the skin, and external pathogenic bacteria can easily enter the body directly, causing skin infection or even necrosis. The consequences of large-scale skin damage are more serious. [0003] Skin wound dressing is a common treatment method for skin injuries. It has the characteristics of water absorption, moisturizing, and breathability. It can form a temporary barrier on the wound, separate the sk...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/26A61L15/28A61L15/32A61L15/24A61L15/20A61L15/44A61L15/42C08J3/24C08L77/04C08L5/08C08L89/00C08L29/04
CPCA61L15/20A61L15/24A61L15/26A61L15/28A61L15/325A61L15/42A61L15/44A61L2300/232A61L2300/404A61L2300/80C08J3/246C08J2305/08C08J2389/00C08J2477/04C08L77/04C08L5/08C08L89/00C08L29/04
Inventor 孙广炜刘洋张英赵姗
Owner 大连敏慧精益科技有限公司
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