The invention relates to a method for improving the
protein solubility of walnut
meal by combining ultrasonic-assisted restrictive enzymolysis with
glycosylation modification, which comprises the following steps:
degreasing walnut
meal to obtain walnut degreased
powder; the method comprises the following steps: dissolving walnut defatted
powder by using deionized water, treating the walnut defatted
powder by using double-frequency ultrasonic
waves, magnetically stirring, centrifuging, taking supernatant, stirring, centrifuging, taking precipitate, and carrying out vacuum
freeze drying to obtain walnut
meal protein; carrying out restrictive enzymolysis on the walnut meal
protein by using
trypsin, and inactivating
protease contained in enzymatic
hydrolysate after enzymolysis; and adding the inactivated enzymatic
hydrolysate into
maltodextrin for a
glycosylation reaction, after the
glycosylation reaction, collecting a solution, freeze-
drying to obtain a
glycoprotein conjugate, and storing under a
drying condition. According to the method disclosed by the invention, ultrasonic-assisted restrictive enzymolysis is combined with glycosylation modification to improve the
protein solubility of the walnut meal, so that the reaction steps can be optimized, the reaction time is saved, the
protein solubility is greatly improved, and the
deep processing and comprehensive utilization of the walnut industry are further promoted.