This invention belongs to the field of bioengineering technology, specifically relating to a preparation process for nano-sized water-soluble
squalene. The process includes the following steps: S1,
corn flour is treated and then mixed with pure water, followed by
enzymatic hydrolysis using a compound
enzyme to obtain a corn saccharification liquid for later use; S2, *
Saccharomyces cerevisiae* GS-A3 is inoculated into a culture medium containing the corn saccharification liquid for
fermentation; S3, after
centrifugation of the
fermentation broth, crude
squalene is obtained by supercritical CO2 extraction; S4, after molecular
distillation, the product is nano-sized. This invention uses corn as
raw material, utilizing abundant
starch resources, converting it into glucose through
enzymatic hydrolysis, and producing
squalene in *
Saccharomyces cerevisiae* via the MVA pathway. This invention prepares high-yield, high-purity squalene, and through a special nano-saccharification process, improves the stability of squalene, extends its
shelf life during application, and significantly improves its
bioavailability. Furthermore, the technology used in this invention meets the requirements of green
environmental protection.