Bacteriostat composition applicable to plant tissue culture and using method thereof

Abstract

The invention relates to a bacteriostat composition applicable to plant tissue culture. The bacteriostat composition comprises the following components in percentage by volume: 2.5 percent of epsilon-polylysine, 22 to 29.5 percent of isothiazolinone, 10 to 15 percent of traditional Chinese medicine water extracts, 2.5 percent of potassium sorbate, sodium benzoate and the like, 3.5 percent of stabilizing agent and 47.0 to 59.5 percent of deionized water. When the composition is used for disinfecting explants of plants, the explants are subjected to routine disinfection after being soaked in the bacteriostat, so that the treatment time on corrosive sublimate or sodium hypochlorite can be reduced and the aims of sterilizing and keeping relatively high survival rate of the explants are fulfilled; the bacteriostat is added into a first generation culture medium or a subculture medium, so pollution of fungus and bacteria can be effectively reduced and prevented and the bacteriostat does not have adverse effect on the plant growth; and when applied to plant open culture, such as a strong seeding and rooting stage and the like, the bacteriostat composition can save the culture medium high-pressure sterilizing process, does not need a clean bench, can save energy and reduce the cost of tissue culture seedlings, and improves benefits.

Description

technical field [0001] The invention belongs to plant tissue culture technology in the field of biotechnology, and relates to a bacteriostat composition suitable for plant tissue culture and a use method. Background technique: [0002] The factory production of plant tissue culture seedlings is developing rapidly in our country, and the techniques of tissue culture are becoming more and more perfect. However, different degrees of contamination often occur in tissue culture. It is understood that many scholars have difficulty obtaining sterile vaccines at the stage of primary culture, or although sterile vaccines have been obtained in primary culture, a large amount of contamination or even complete pollution often occurs during subculture. Therefore, controlling or reducing the pollution rate is a key technical link that cannot be ignored in the factory production of plant tissue culture, and it is also one of the important indicators to measure the efficiency of tissue cul...

AI Technical Summary

Problems solved by technology

However, different degrees of contamination often occur in tissue culture

It is understood that many scholars have difficulty obtaining sterile vaccines at the stage of primary culture, or even though they have obtained sterile vaccines in the primary culture, a large amount of contamination or even complete pollution often occurs during subculture.

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