198 results about "Collagen gel" patented technology

A method of preparing a collagen sponge comprises mixing air into a collagen gel, so as to obtain a collagen foam which is dried. From the dried product thereby obtained, collagen sponge is obtained by isolating parts of sponge with a chamber diameter of more than 0.75 mm and less than 4 mm, or parts with an average chamber diagonal dimension of 3 mm. The collagen sponge may be used as a material for sealing wounds, possibly with a coating comprising a fibrin glue, such as a combination of fibrinogen, thrombin and aprotinin. A device for extracting a part of a collagen foam and for degenerating another part of the collagen foam to a collagen gel is disclosed. An elongated collagen sponge having a through-going hole or bore and a flexible wall may be used for re-establishing walls in a mammalian gastrointestinal funnel or trachea system.

The invention provides dialdehyde carboxymethyl cellulose-collagen frozen gel and a preparation method thereof. The method comprises the following steps of: first uniformly mixing 1 to 3 mass percent of the aqueous solution of collagen and 0.01 to 1 mass percent of the aqueous solution of dialdehyde carboxymethyl cellulose; then injecting the mixed solution into a mold and storing the mixed solution in a low-temperature reactor at the temperature of -40 to 0 DEG C for 1 to 7 days; and finally, taking the obtained product out and slowly unfreezing the obtained product to obtain the dialdehyde carboxymethyl cellulose-collagen frozen gel. The dialdehyde carboxymethyl cellulose-collagen freezing gel prepared by the method is improved in mechanical property, thermal stability and enzymatic degradation resistance at the same time of maintaining the biological activity of collagen gel per se, has the advantages of porosity, hydrophilicity, water absorbing and preserving property, good market application prospect and the like, and is widely used in biomedical fields, such as biologic scaffolds, cell culture, drug delivery, tissue engineering, wound and burning treatment and the like.

The invention provides a collagen matrix freezing gel used for biomedical materials and a preparation method thereof. The collagen matrix freezing gel is the collagen extracted from skins or tendons of healthy domestic animals using the enzyme method with the molecular weight of 280 to 320kDa and a well maintained triple helical structure; the collagen reacts for 1 to 7 days in the condition of low temperature of 0 to minus 50 DEG C in a die after through cross linked and modified reaction with hydroformylation polysaccharide, then extrudes the die and is defrosted, thus forming the collagen matrix freezing gel. The preparation of the collagen matrix freezing gel of the invention in particular relates to the extraction of the collagen, the preparation of the hydroformylation polysaccharide and the synthesis of hydroformylation polysaccharide-collagen matrix freezing gel. The hydroformylation polysaccharide-collagen matrix freezing gel prepared by the invention improves the mechanical property, thermal stability and anti-enzyme degradation, etc., of pure collagen gels, and the freezing gel has the advantages of porosity, plasticity, hydrophilic property and non-toxicity, and can be used as biomedical materials such as bio-scaffold, cell cultivation, drug controlled release and biological dressings.

The invention provides a manufacturing technology of a 3D printed mask. The technology includes the following steps that under sterile vacuum conditions, according to human facial profiles, collagen gel and mask essence are sequentially stacked and printed through a 3D printer to obtain the 3D printed mask. The mask is manufactured through the 3D printing technology, the mask can be obtained by sequentially stacking and printing the collagen gel and the mask essence, the method is simple, and consumed time is short. Besides, the high-temperature process is not needed during printing, and effective components of the mask essence can be completely stored. Besides, a porous mask support is formed through the collagen gel in the vacuum condition, and the mask essence can be adsorbed to the largest extent. The obtained 3D printed mask is specialized according to individuation of the human facial profiles, and can completely fit with pores on human face skin.

The invention is based on selective inhibition of the enzyme (MMP-1), which causes the dermal matrix damage in humans, while sparing the enzyme(s) (MMP-9 and perhaps MMP-2) which not only do not cause the damage (based on extrapolation from our in vitro collagen gel system to real skin) but actually "clear away" the damage produced by MMP-1 to restore normal function to the skin. Matrix metalloproteinase-1 (MMP-1; fibroblast collagenase) is induced by UV radiation from the sun and is naturally elevated in old age. Human fibroblasts exposed to the degradation products of MMP-1 contract collagen, but when this debris is removed from their environment, the fibroblasts behave normally. Inhibiting MMP-1 but sparing enzymes that remove the debris improves human skin after onslaught from solar UV radiation, old age, and acne.

The invention relates to a method of in vitro testing of the efficacy of a potentially active substance comprising monitoring the effect of said potentially active substance on an artificial skin, comprising a composite product forming a collagen support comprising at least one porous collagen layer covered on at least one side with a collagen membrane component selected from the group consisting of a collagen membrane prepared by compression of a collagen sponge at a pressure of at least about 50 bar and of a collagen membrane comprising a collagen film prepared by drying a collagen gel separately from the porous collagen layer, thereby providing a reliable method for finding new potentially active substances.

The invention relates to high-strength collagen gel. The collagen gel is characterized in that the high-strength collagen gel undergoes three-dimensional size shrinkage by immersing radiation cross-linked low-density collagen hydrogel in water or an aqueous solution for a period of time and maintaining a certain temperature; the compression modulus is increased by 2-5 times that of the low-density collagen hydrogel after self-shrinkage by solution immersion; and the high-strength collagen gel has a three-dimensional structure or high porosity, and the pore diameter is distributed in a gradient manner. The invention further relates to a method for preparing the high-strength collagen gel.

The invention provides a preparation method of a bionic mineralized collagen scaffold. The preparation method comprises the following steps: 1), preparing a bionic mineralized liquid: adjusting the pHvalue of carboxymethyl chitosan-amorphous calcium phosphate (or amorphous strontium phosphate or amorphous strontium carbonate) nanocomposite liquid to be lower than an isoelectric point of CMC; 2),preparing the bionic mineralized collagen scaffold: mixing the bionic mineralized liquid and acid-dissolved type-I collagen, then putting into a dialysis bag, sealing, then putting into a PBS buffer liquid, self-assembling and mineralizing for 2-5 days, then adding an alkaline CMC/ACP (ASP or ASC) liquid into the dialysis bag, and changing the PBS buffer liquid; 1-3 days later, putting the dialysis bag into deionized water for dialyzing for 10-72h, changing the deionized water for several times, centrifuging the liquid in the bag to obtain collagen gel, and performing freeze drying to obtain the bionic mineralized collagen scaffold. By the preparation method, collagen self-assembly and mineralization are cooperatively performed, intra-collagen fiber mineralization is achieved at relativelyhigh efficiency, and osteogenic ability-promoting strontium is integrated into the collagen scaffold, so that the preparation method has a wide clinical application prospect.

The invention relates to the technical field of medical gels, and in particular relates to a recombinant human collagen gel and a preparation method thereof. The recombinant human collagen gel consists of the following raw materials in percentage by weight: 1-3% of small-molecule collagen, 1.5-2.5% of glycerol, 1-2% of chitosan quaternary ammonium salt, 0.5-1.5% of sodium hyaluronate, 0.5-1.5% of epsilon-polylysine, 0.3-0.7% of borneol, 0.3-0.7% of poloxamer, 0.3-0.7% of coenzyme Q10, 0.3-0.7% of butanediol and the balance of purified water. The recombinant human collagen gel provided by the invention has effects of moisture retention and isolation, can improve vaginal atrophy, increase vaginal elasticity and relieve dyspareunia, and also can improve the wettability and the dry state of a vagina, relieve dry and coarse stabbing pain symptoms of cunnus, and ease itching, calor, skin mucous membrane fissure and diffuse superficial bleeding caused by vaginal dryness.

The invention discloses a preparation method of collagen sponge. The method comprises the following steps: carrying out enzymolysis on cattle heel tendon in protease aqueous solution at pH value of 1.0-5.0; centrifuging the enzymolysis liquid, collecting supernatant, and salting out collagen with saturated salt solution; dialyzing salted-out collagen; cross-linking the dialyzed collagen in a cross-linking agent aqueous solution; and freeze-drying cross-linked collagen gel, cross-linking the collagen in a cross-linking agent aqueous solution and freeze-drying to obtain the collagen sponge. The collagen sponge obtained by the preparation method of the collagen sponge disclosed by the invention has certain elastic and tensile toughness after being immersed in water, and has good biocompatibility and tissue repairing performance, and high protein content and is controllable in degradation.

The invention relates to a method for preparing a high-strength collagen tissue repair material product. The method obtains the final collagen product through the treatment steps of salting out, centrifugation, dehydration, crosslinking and drying of a collagen solution. Because the treatment method adopts the mode of improving the solid content of collagen in collagen gel and the crosslinking density in the collagen gel, the mechanical property of the final product is greatly improved. Simultaneously, because the adopted method does not influence the biological property of the collagen, the final collagen product still maintains good biological activity. The collagen product can be used for the repair treatment of articular cartilage, meniscus and skin, the construction of artificial blood vessels and nerve conduits, and the like.

An artificial collagen gel skin is prepared from ox tendon collagen dispersing liquid 80 portions, DMEM culture liquid 10, glutamine solution 1, dicarbonate solution 6 and fetal calf serum 12 through proportionally mixing, adding human embryo skin fibroblast suspension, and external culture.

The invention discloses a collagen gel containing nanogold, and a preparation method and an application thereof. The gel is prepared by the method of reducing a gold precursor by collagen. Firstly, reducing groups in collage react with the gold precursor to generate nanogold, then the generated nanogold further crosses link a three-screw structure of collagen in situ, to form compact gel. The property stability of the collagen is even, the particle size of contained nanogold is controllable, the preparation process is simple and convenient, chemical cross linking agent is not required, and so the collagen gel has a wide application prospect in the fields of daily cosmetic antioxidant masks and the like.

The invention relates to an aquatic collagen fish bean curd and a preparation method thereof. The fish bean curd is composed of the following raw materials by mass: 65-90% of aquatic collagen glue peptone, 2.5% of soybean isolate protein, 0.1-0.5% of a protein cross-linking agent, 0.5-2% of egg white protein, 1-5% of vegetable protein, 0-10% of starch, 1-8% of pork fat, 0.1-2% of thermal reversible colloid, 0.1-1% of thermal irreversible glue, and 2-10% of a seasoning. The preparation method of the fish bean curd comprises: preparing the raw materials into a collagen gel peptone shape, then adding soybean isolate protein to perform pulping and adding the protein cross-linking agent to conduct chopping, then adding auxiliary materials and the seasoning, mixing the materials evenly and carrying out rough processing shaping, finally conducting deep processing to obtain a finished product and then carrying out vacuum packaging, sterilizing, cooling, encasement and warehousing. The fish bean curd provided by the invention has the characteristics of high protein content, rich nutrition and convenient eating, and solves the technical barrier that traditional collagen products have poor heat resistance. The preparation method of the fish bean curd provided by the invention has the advantages of simple and convenient operation and high efficiency, and is suitable for popularization and application.

To provide a semaphorin inhibitor; a peripheral or central nerve regeneration promoter which contains said semaphorin inhibitor as an active ingredient; and a preventive or remedy for a neuropathic disease and a neurodegenerative disease containing said nerve regeneration promoter, or the like.

A low-molecular weight compound, which acts at a concentration of 10 μg/ml or below to inhibit the growth cone collapse activity of semaphorin such as semaphorin 3A, semaphorin 6C or the like and/or the nerve outgrowth inhibitory activity of semaphorin in a collagen gel and which does not substantially affect cell proliferation, is obtained from the culture of strain SPF-3059 belonging to the genus Penicillium. The low-molecular weight compound with the semaphorin inhibitory activity thus obtained exhibits the in vivo nerve-regeneration promoting action.

The micro hollow fiber cell reactor consists of silica gel tube of permeable silica gel material, permeable silica gel stopper and hollow fiber filament. Cell is embedded inside the hollow fiber filament with collagen gel, and the micro hollow fiber cell reactor has oxygen transferring and mass transferring capacity suitable for cell to survive and maintains the medicine metabolizing and other functions of cell. The micro hollow fiber cell reactor combines the advantages of miniature orifice plate and 3D bionic artificial liver reactor, maintains the bioactivity of animal cell. It may be used as in vitro mold of liver cell for the research of medicine metabolism and pharmacological and toxicological research and may be used in the lab research of cell culturing condition and cell product. It may be also used as the carrier for the research of animal cell culturing condition and lays the foundation for industrial production.