88 results about "Collagen gel" patented technology

The present invention relates to a preparation method of a collagen filler for injection. Non-crosslinked collagen, crosslinked collagen, and biodegradable polymer microspheres are compounded and mixed in a certain proportion to achieve an immediate filling effect. The biodegradable polymer microspheres play a long-term role, and the amino acid components degraded from collagen in the early and middle stages can become nutrients that stimulate the production of collagen regeneration by polyester microspheres. In the present invention, the crosslinked collagen has a high yield and less water loss through a gradient irradiation crosslinking technique. The non-crosslinked collagen gel can provide a microenvironment for nutritional repair and a filling effect in the early stage, the crosslinked extracellular matrix can provide a repair microenvironment in the middle stage of filling, and the degradation of polyester microspheres in the later stage promotes collagen regeneration, enabling the skin to have a good microenvironment throughout the repair cycle and increasing skin elasticity.

The present invention provides a chitosan recombinant humanized collagen gel, comprising the following components in mass percentage: 0.08-0.30% of type III recombinant humanized collagen; 0.20-0.45% of chitosan or its derivatives; 0.05-0.13% of a cross-linking agent; 0.10-0.20% of a moisturizing agent; and the balance is water. The dressing provided by the present invention can slowly release humanized collagen, is non-toxic, non-irritating, has high biocompatibility, good stability, and can continuously promote the healing of uterine incisions.

The invention provides a recombinant III-type humanized collagen gel based on a macromolecular crowding effect as well as a preparation method and application of the recombinant III-type humanized collagen gel. The method comprises the following steps: firstly, preparing an rhCol III solution system; then preparing a macromolecular crowding bath containing dextran 70, polyethylene glycol 8000 and ascorbic acid; then adding an rhCol III solution into the macromolecular crowding bath to form an rhCol III microgel; and finally, constructing a 3D printing molecular exclusion mold system which takes agarose as a substrate and contains a functional additive and a temperature-sensitive polymer as a temperature-sensitive release and spatial constraint component, so as to realize directional construction of the rhCol III microgel. According to the method disclosed by the invention, second-level physical gelling (lt; 15s) of rhCol is realized, and a physiological microenvironment supporting cell proliferation is constructed. The recombinant III-type humanized collagen gel prepared by the method can be used for preparing collagen microgel balls, collagen bundles, 3D (three-dimensional) bio-printing products and the like, and is wide in application range.

The invention belongs to the technical field of biomedical treatment, and particularly relates to a preparation method of collagen and hyaluronic acid co-crosslinked gel. Comprising the following steps: (1) mixing sodium hyaluronate with a first part of cross-linking agent solution and a first part of alkali liquor, and carrying out pre-crosslinking reaction to obtain pre-crosslinked hyaluronic acid gel; mixing collagen with a second part of the cross-linking agent solution and a second part of the alkali liquor, and carrying out a pre-crosslinking reaction to obtain pre-crosslinked collagen gel; and (2) stirring and blending the pre-crosslinked hyaluronic acid gel and the pre-crosslinked collagen gel, adding the residual alkali liquor in the stirring and blending process, carrying out a co-crosslinking reaction, and purifying to obtain the collagen and hyaluronic acid co-crosslinked gel. A multi-dimensional network structure is constructed through a synergistic cross-linking technology of sodium hyaluronate and collagen, so that the mechanical strength and the structural stability of the gel are remarkably enhanced, the problem of phase separation easily occurring in a traditional material is effectively solved, and the gel is suitable for the field of medical cosmetology.

The invention relates to the technical field of medical cosmetology, in particular to a porous hollow glass bead capable of slowly releasing collagen and application of the porous hollow glass bead. The porous hollow glass bead capable of slowly releasing the collagen comprises a porous hollow glass bead body, the outer wall of the porous hollow glass bead body is provided with a pore channel structure penetrating through the exterior and an inner cavity of the hollow glass bead body, and the pore channel and the inner cavity of the porous hollow glass bead body are filled with collagen gel. The compressive strength of the hollow glass beads is utilized to improve the mechanical supporting effect, the collagen gel is subjected to catalytic decomposition under the action of collagenase in a body so as to achieve the slow-release effect, the outer walls of the hollow glass beads slow down the reaction time through physical isolation, and then the slow-release time is prolonged; meanwhile, ions such as Si and Ca released by the hollow glass beads can promote cells to secrete collagen, the biological activity is improved, and the three beneficial effects of physical support, collagen slow release and biological safety are achieved.

The invention belongs to the technical field of colloid filling, and particularly relates to recombinant collagen gel subpackaging equipment which comprises a bearing part, a material injection mechanism, a preheating mechanism and a recycling mechanism. The bearing part comprises a bearing disc, a supporting frame and a bearing plate, the bearing disc is fixedly mounted on the outer wall of the supporting frame, and the bearing plate is slidably arranged on the outer wall of the supporting frame; the material injection mechanism comprises a buffer box, an adapter block and a material injection pipe, the buffer box is fixedly mounted on the upper end face of the bearing plate, and the material injection pipe communicates with an inner cavity of the buffer box through the adapter block; the preheating mechanism comprises a storage box and a wrapping pipe, the storage box is fixedly installed on the side wall of the bearing plate, a heating piece is arranged in the storage box, and the wrapping pipe is arranged outside the material injection pipe; the recycling mechanism comprises a backflow box and a condensation plate; the condensation plate is fixedly mounted on the inner wall of the backflow box; through cooperation of the structures, the filling process is smoother, the filling efficiency is improved, material residues on the inner wall of the material injection pipe are reduced, and the risk of bacterium breeding after shutdown is reduced.

The invention discloses a high-throughput drug screening method, device, equipment and medium, and relates to the technical field of drug screening, highly abstract semantic information in a collagen gel image is accurately extracted through sampling branches under a deep neural network, and the microstructure of collagen gel can be more accurately identified and analyzed; extracting low-dimensional resolution information in the collagen gel image through an up-sampling branch, combining the highly abstract semantic information and the low-dimensional resolution information into detail information, and segmenting each pixel in the collagen gel image step by step based on the detail information to obtain a collagen gel area; according to the segmentation method combined with the detail information, the collagen gel area can be identified more accurately, influence factors during acquisition of the collagen gel image are counteracted, and the collagen gel area is accurately segmented so as to be better applied to high-throughput drug screening.

The present invention discloses a preparation method of an active injectable gel that can withstand irradiation sterilization. The active injectable gel is composed of a collagen gel extracted from an animal source, acellular dermal matrix microparticles, bacterial cellulose, glycerol, and amino acids compounded in a certain proportion, and serves as a filling material that plays an exogenous filling and endogenous collagen regeneration role. Through the protection of glycerol and amino acids and the method of cryopackaging with liquid nitrogen or dry ice, the collagen gel can still maintain its activity and gel properties after being sterilized by Co60 irradiation, and achieve the effects of sterility and virus inactivation. By adding acellular dermal matrix microparticles and bacterial cellulose, the injection force is increased and the action time of the pure collagen gel is effectively extended to achieve a long-term effect.

The invention discloses a filling material for promoting tissue regeneration and a preparation method thereof. The filling material is prepared by using type II collagen as a microsphere wall material and hydroxyapatite as a core material and dispersing composite microspheres in type I collagen gel. The filling material for promoting tissue regeneration prepared by the invention is used for filling parts such as eyebrow lines, eyebrow subcutaneous support, zygomatic fat and cheek fat pads, lacrimal ducts, nasolabial folds, noses, lips, chin, mandible periosteum and the like, has an obvious mechanical support effect, continuously stimulates the formation and rearrangement of endogenous collagen on the basis of extrinsic collagen supply, and can be used for promoting tissue regeneration. The medical and aesthetic plastic requirements are met.

The invention relates to the technical field of biological materials, in particular to collagen gel for injection, a preparation method of the collagen gel and an injection preparation. The method comprises the following steps: providing recombinant humanized collagen subjected to alkali resistance screening, and mixing the recombinant humanized collagen with a stabilizer to prepare a collagen dispersion liquid, which comprises the following steps: activating a cross-linking agent in an alkaline activating solution to obtain an activated cross-linking agent solution; wherein the cross-linking agent is a diepoxide compound; carrying out cross-linking reaction on the activated cross-linking agent solution and the collagen dispersion liquid to form gel; wherein the mass concentration of the collagen dispersion liquid is 5-10%, and the mass ratio of the cross-linking agent to the collagen is 5-10%; the total weight percentage of the collagen and the cross-linking agent is 5.5 to 10.5 percent. The gel with a stable structure can be effectively formed when the content of the cross-linking agent is within the content range, the residual amount of the cross-linking agent is low, and the storage modulus, the swelling degree and the pushing force of the gel are excellent.

The invention provides a recombinant collagen gel material as well as a preparation method and application thereof. The preparation method of the material comprises the following steps: mixing recombinant collagen with a first water phase to obtain a recombinant collagen solution, and drying and granulating to obtain first recombinant collagen microspheres; performing physical crosslinking on the first recombinant collagen microspheres to obtain second recombinant collagen microspheres; mixing and stirring the second recombinant collagen microspheres and a second water phase, and swelling to obtain a white dilute solution with suspended particles; and carrying out low-temperature irradiation crosslinking on the white dilute solution with suspended particles to obtain the recombinant collagen gel material. According to the preparation method disclosed by the invention, the production period is shortened, the production cost is greatly reduced, and the gel material prepared by the method is high in biological safety and has good mechanical property and degradation property.

The invention provides III-type recombinant collagen, belongs to the technical field of biology, and comprises an amino acid sequence of the III-type recombinant collagen as shown in SEQ ID NO.1. The invention also provides a preparation method of the III-type recombinant collagen. According to the technical scheme provided by the invention, the purification process is simplified, the production cost is reduced, and the complex recombinant collagen repair gel provided by the invention can repair the skin barrier and relieve dry itching and stabbing pain of the skin.

The invention relates to injectable cross-linked collagen gel and a preparation method thereof. Two crosslinking strategies of self-crosslinking and crosslinking agent crosslinking are fused together for the first time, and two types of amido bonds, namely amido bonds of a collagen-collagen structure (A) and amido bonds of a collagen-fatty amine-collagen structure (B), are respectively constructed through two crosslinking paths, namely catalysis of collagen polypeptide self-crosslinking and catalysis of fatty amine crosslinking, so that a hard / soft dual crosslinking network is obtained. The relative proportion of the structure A and the structure B in the final product is adjusted through a proper cross-linking process, so that the strength and the stability of the final product are adjusted and controlled, and the use requirements of different injection parts can be met.

The invention discloses low-temperature stable compound temperature-sensitive recombinant humanized collagen gel as well as a preparation method and application thereof. The compound temperature-sensitive recombinant humanized collagen gel comprises the following components in percentage by weight: 17%-20% of poloxamer 407, 0.1%-0.4% of recombinant humanized collagen, 10%-20% of glycerol, 1%-5% of a humectant and the balance of water. The stability of the recombinant humanized collagen gel is improved by adding moisturizers such as glycerol and PEG-8 into a poloxamer 407 gel system, the controllable adjustment of the gel forming temperature of the gel is realized by adjusting the composition proportion, and the gel forming temperature of the gel is regulated and controlled to be 10-30 DEG C. The compound temperature-sensitive recombinant humanized collagen gel disclosed by the invention can stably form gel under different acidic and neutral pH conditions, has good cell compatibility, is free of vaginal irritation and mild implantation reaction, and has wide application prospects in the fields of vaginal in-situ gel medical instruments, biological materials and the like.

The invention provides cross-linked recombinant collagen gel. The cross-linked recombinant collagen gel consists of the following components in percentage by mass: 0.4-1.6% of recombinant III-type humanized collagen; 0.2%-2% of a D-core; 0.1% to 0.5% of carbomer; 0.1% to 0.6% of triethanolamine; 1.0%-10% of glycerol; the collagen gel disclosed by the invention has the advantages of low immunogenicity, high safety, no toxicity, no stimulation, good biocompatibility, structural stability, collagen enzymolysis resistance and the like, and meanwhile, the invention also provides a preparation method and application of the cross-linked recombinant collagen gel.

The present application discloses a thermally stable recombinant collagen gel and its preparation process, which relates to the technical field of recombinant collagen. The raw materials include, by mass percentage, 10 - 20% water, 40 - 50% modified recombinant collagen, 13 - 20% traditional Chinese medicine preparation, 8 - 16% sodium hyaluronate, 2 - 4% composite heat stabilizer, 1 - 2% preservative, and the balance is glycerol; the modified recombinant collagen is thiolated modified recombinant collagen; the composite heat stabilizer includes trehalose and pomegranate peel polyphenols, and the mass ratio of the two is 1:0.4 - 0.8. The present application significantly improves the thermal stability of the recombinant collagen gel by thiolating and modifying the recombinant collagen and adding a composite heat stabilizer to the gel components.

The invention discloses a composite rotator cuff patch and a preparation method thereof. The composite rotator cuff patch is divided into an electrostatic spinning layer and a collagen layer. The electrostatic spinning nanofiber membrane is coated with a layer of crosslinked collagen gel, and then composite freeze drying is performed to obtain the composite rotator cuff patch. The rotator cuff patch has good biocompatibility and appropriate tissue adhesion, does not need to be sutured and fixed, can be degraded in vivo, has degradation time close to rotator cuff repair time, and does not need to be taken out after an operation.

Systems, methods, and devices for internal wound treatment include a hemostasis device. The hemostasis device has an insertion tube extending from a base portion. One or more openings are formed into a surface of the insertion tube. Additionally, the hemostasis device includes an inflation pump couplable to a proximal of the insertion tube for pumping air into an inner channel of the insertion tube. A wound channel balloon is disposed at least partially around the insertion tube such that an actuation of the inflation pump causes the air to flow from the inner channel through the one or more openings and inflate the wound channel balloon. The wound channel balloon can include a grooved elastic fiber, one or more bulges, and / or one or more branches. Furthermore, a collagen gel, an antibiotic, a blood clotting agent, an analgesic, stem cells, a sealant, and / or a lubricant can be disposed on the wound channel balloon.

The invention discloses a collagen implant containing polynucleotide as well as a preparation method and application thereof, and relates to the technical field of implants. The collagen implant containing polynucleotide is in a gel state and is formed by mixing polynucleotide gel and collagen gel, the content of collagen in the implant is 25 to 40 mg / mL, and the content of polynucleotide in the implant is 5 to 20 mg / mL. The implant formed by collagen and polynucleotide in a proper ratio has high viscosity, can play a good anti-wrinkle effect, and is good in stability and not prone to bleeding and layering.

The invention discloses an anti-aging three-dimensional high-uniformity dermis evaluation model based on image analysis, and belongs to the technical field of biological medicine and cosmetic efficacy evaluation. The method comprises the following steps: mixing a prepared fibroblast suspension and a neutralized collagen solution at 0-5 DEG C, inoculating the mixture into a 24-pore plate in a 100-200L / pore manner, standing in an incubator at 37 DEG C for 20-40 minutes to form collagen gel, carrying out suspension culture to obtain a 3D dermal model, and detecting the shrinkage rate of the model and evaluating the anti-aging effect in combination with a high-content cell imaging system. The method overcomes the defect that a traditional method is large in error, an efficient and accurate evaluation system is provided for anti-aging drug screening, and the method has application value in cosmetic effect detection and skin aging mechanism research.

The invention discloses a recombinant humanized collagen gel preparation and a preparation method and application thereof, and relates to the technical field of daily chemicals. The invention uses grape seed extract and silanized hyaluronic acid ester (SHAC) in combination on the basis of recombinant humanized collagen, which can supplement collagen fibers in the skin, promote the proliferation of human skin keratinocytes and fibroblasts and collagen synthesis, inhibit collagen degradation, repair the skin, enhance the skin barrier and moisturize; inhibit the cross-linking effect of collagenase and elastase on connective tissue, improve skin immunity, and maintain skin elasticity; and has good antioxidant properties, can neutralize free radicals, effectively prevent skin aging, thereby obtaining excellent moisturizing and anti-wrinkle properties. The recombinant humanized collagen gel preparation of the invention can promote fibroblast proliferation, has excellent moisturizing properties, and can significantly improve skin elasticity, and can be used in various moisturizing and anti-wrinkle skin care products.

The invention discloses recombinant III-type humanized collagen gel and a preparation process thereof, and belongs to the field of biological medicines and high polymer materials. The gel aims at solving the problems that in the prior art, collagen gel is weak in mechanical property, short in maintaining time and single in biological activity. The key point of the technical scheme is that the gel has a double-network interpenetrating structure, and the structure comprises a first network formed by recombinant III-type humanized collagen cross-linked by an enzyme method and a second network which is physically interpenetrated with the first network and is formed by thermo-sensitive macromolecules (such as poloxamer). Preferably, slow-release nanoparticles loaded with growth factors (such as bFGF) are also loaded in the gel so as to actively induce tissue regeneration. According to the preparation process, the components are mixed at low temperature, and a first network and a second network are sequentially formed through an enzymatic reaction and temperature response two-step method. The gel disclosed by the invention has excellent mechanical supporting property, long-acting stability and active biological activity, and is suitable for the fields of medical beauty filling and tissue repair.

1.The name of the design product: packaging box (Wei Mei Hive Col 164.88° vertical box three). 2.The use of the design product: packaging box for injection of recombinant humanized type III collagen gel. 3.The design points of the design product: the combination of shape, pattern and color. 4.The picture or photo that best indicates the design points: perspective view. 5.The design for which protection is sought contains color.

This invention relates to the field of biomedical materials technology and discloses a recombinant collagen gel and its preparation method. The preparation method is as follows: recombinant collagen is dissolved in glacial acetic acid solution and stirred until completely dissolved to obtain a recombinant collagen solution. The recombinant collagen solution is added to phosphate buffer, and then modified chitosan, phenylboronic acid-modified sodium alginate, and glycerol are added. The mixture is stirred for 30-40 minutes to obtain a gel precursor solution. An aldehyde-modified epoxy-thiol polymer is dissolved in dimethyl sulfoxide to prepare a stock solution. The stock solution is then added dropwise to the gel precursor solution, and the mixture is stirred for another 5-10 minutes. The mixture is injected into a sterile mold, allowed to stand for 12-24 hours, defoamed, sterilized, and then sealed and stored at 4°C to obtain the recombinant collagen gel. The recombinant collagen gel prepared by this invention has good mechanical properties, self-healing properties, is safe and non-toxic, and has high biocompatibility.

The present invention discloses a preparation method and application of a carbonate mineralized collagen scaffold material. The preparation method is to first immerse the collagen gel in reagent A, the reaction temperature is 25-37°C, the reaction time is 1-24 hours, and after the reaction is completed, the excess A liquid on the surface of the collagen gel is removed, and the collagen gel is mixed with reagent B for reaction, the reaction temperature is 25-37°C, and the collagen gel is taken out and thoroughly rinsed, and dried to obtain a carbonate mineralized collagen scaffold material. Reagent A is a soluble carbonate and bicarbonate solution with a pH of 9.5-10, and reagent B is a polyelectrolyte-metal cation suspension. The carbonate mineralized collagen scaffold material prepared by this method has a structure in which the inside and outside of the collagen fiber are mineralized simultaneously, and has good biocompatibility and degradability, and slowly and continuously releases functional metal cations by forming insoluble carbonates in collagen to promote bone defect repair.

The invention relates to the technical field of biological medicine, in particular to a collagen gel delivery system integrated with polypeptide medicine and a preparation method and application thereof.The collagen gel delivery system is characterized in that collagen serves as a basic gel matrix, covalent cross-linking is conducted through 4-arm-PEG-NHS, then active polypeptide is connected into a gel network through amido bonds, and the collagen gel delivery system integrated with the polypeptide medicine is obtained. The active polypeptide is an antibacterial peptide and / or an MMP-9 cyclopeptide inhibitor. The collagen gel delivery system is expected to realize the efficient and ordered healing promotion effect on the wound infected by diabetes mellitus through a multi-target synergistic mechanism such as anti-infection, anti-inflammation, collagen degradation inhibition and collagen synthesis promotion, and provides a new idea and a new option for clinical response.

The present application relates to a kind of frozen pre-made grass carp fillets method, belong to food preservation technical field.The frozen pre-made grass carp fillets method disclosed in the present application, mainly using quality improvement liquid (including: 3~4% collagen gel liquid, 0.4~0.6% glutamine transaminase (TG enzyme), 2~4% salt according to weight percentage) is injected into fish meat to be pickled.Then subsequent processing can be done, the salt in the quality improvement liquid can promote the unfolding of myofibrillar protein structure in fish meat, collagen gel liquid can fill the intercellular pores left after myofibrillar protein structure changes, glutamine transaminase can regulate the crosslinking degree and thermal stability of collagen gel to make it have the characteristics of intermuscular matrix protein, the above effects can inhibit the damage of fish meat cells and protein structure caused by endogenous enzymes and ice crystals during freeze-thaw process.The method used in the present application can reduce the quality deterioration of fish meat during freeze-thaw process, so that the fish meat can maintain better taste after frozen storage.

The application provides a recombinant type III humanized collagen gel based on macromolecular crowding effect and a preparation method and application thereof. First, an rhCol III solution system is prepared; then a macromolecular crowding bath containing dextran 70, polyethylene glycol 8000 and ascorbic acid is prepared; then the rhCol III solution is added into the macromolecular crowding bath to form rhCol III microgel; finally, a 3D printing molecular exclusion mold system with agarose as a base, containing functional additives and a temperature-sensitive polymer as a temperature-sensitive release and space-restricted component is constructed to realize directional construction of the rhCol III microgel. The application realizes physical gelation of the rhCol in seconds (<15 s) and constructs a physiological microenvironment supporting cell proliferation. The recombinant type III humanized collagen gel prepared by the application can be used for preparing collagen microgel balls, collagen bundles, 3D biological printing and other products, and has a wide range of applications.

The utility model discloses a recombinant collagen gel stirring device, relates to the technical field of recombinant collagen gel stirring, and solves the problems that due to the specific properties of gel, the interaction between molecules of the gel is relatively strong, a completely uniform state is possibly difficult to realize in the stirring process, and even the concentration of a part of regions is too high or too low, and the like. Comprising a tank body and a supporting mechanism, a stirring mechanism comprises a second servo motor and a rotating rod, an annular rod is fixedly installed on the outer side of the top of the rotating rod, and a first blade and a second blade are fixedly installed on the outer side of the rotating rod and the inner side of the annular rod respectively. Through the stirring movement of the first blade and the second blade which are respectively mounted on the outer side of the rotating rod and the inner side of the annular rod, and the unique design of the second blade stirs the raw materials deposited on the bottom wall of the tank body to lift upwards, so that the uniform state which is difficult to achieve in the stirring process is realized, and the problems of too high or too low concentration of partial regions and the like are avoided.

The invention relates to the technical field of recombinant collagen gel packaging, in particular to recombinant collagen gel packaging sealing performance detection equipment which comprises an air pressure testing assembly, a supporting long frame, a lifting assembly, a conveying assembly, a defective product discharging mechanism, a defective product collecting funnel and a supporting bottom frame. The long supporting frames are arranged at the middle end of the upper portion of the supporting bottom frame at intervals, the two ends of each long supporting frame are correspondingly connected with the two sides of the middle end of the upper portion of the supporting bottom frame through lifting assemblies, and multiple air pressure testing assemblies which are evenly distributed at intervals are arranged in the front side and the rear side of the middle end of each long supporting frame. Conveying assemblies are arranged on the front side and the rear side of the lower end of the long supporting frame at intervals, and defective product discharging mechanisms are arranged on the front side and the rear side of the upper end of the bottom supporting frame. According to the invention, two rows of multi-group air pressure tightness detection assemblies are arranged, whether a plurality of bottle bodies leak can be detected at the same time, the efficiency is high, no pollution is caused to the bottle bodies, and the technological process and the detection time are reduced.