43 results about "Collagen gel" patented technology

The invention provides a recombinant III-type humanized collagen gel based on a macromolecular crowding effect as well as a preparation method and application of the recombinant III-type humanized collagen gel. The method comprises the following steps: firstly, preparing an rhCol III solution system; then preparing a macromolecular crowding bath containing dextran 70, polyethylene glycol 8000 and ascorbic acid; then adding an rhCol III solution into the macromolecular crowding bath to form an rhCol III microgel; and finally, constructing a 3D printing molecular exclusion mold system which takes agarose as a substrate and contains a functional additive and a temperature-sensitive polymer as a temperature-sensitive release and spatial constraint component, so as to realize directional construction of the rhCol III microgel. According to the method disclosed by the invention, second-level physical gelling (lt; 15s) of rhCol is realized, and a physiological microenvironment supporting cell proliferation is constructed. The recombinant III-type humanized collagen gel prepared by the method can be used for preparing collagen microgel balls, collagen bundles, 3D (three-dimensional) bio-printing products and the like, and is wide in application range.

The invention relates to the technical field of medical cosmetology, in particular to a porous hollow glass bead capable of slowly releasing collagen and application of the porous hollow glass bead. The porous hollow glass bead capable of slowly releasing the collagen comprises a porous hollow glass bead body, the outer wall of the porous hollow glass bead body is provided with a pore channel structure penetrating through the exterior and an inner cavity of the hollow glass bead body, and the pore channel and the inner cavity of the porous hollow glass bead body are filled with collagen gel. The compressive strength of the hollow glass beads is utilized to improve the mechanical supporting effect, the collagen gel is subjected to catalytic decomposition under the action of collagenase in a body so as to achieve the slow-release effect, the outer walls of the hollow glass beads slow down the reaction time through physical isolation, and then the slow-release time is prolonged; meanwhile, ions such as Si and Ca released by the hollow glass beads can promote cells to secrete collagen, the biological activity is improved, and the three beneficial effects of physical support, collagen slow release and biological safety are achieved.

The invention relates to the technical field of biological materials, in particular to collagen gel for injection, a preparation method of the collagen gel and an injection preparation. The method comprises the following steps: providing recombinant humanized collagen subjected to alkali resistance screening, and mixing the recombinant humanized collagen with a stabilizer to prepare a collagen dispersion liquid, which comprises the following steps: activating a cross-linking agent in an alkaline activating solution to obtain an activated cross-linking agent solution; wherein the cross-linking agent is a diepoxide compound; carrying out cross-linking reaction on the activated cross-linking agent solution and the collagen dispersion liquid to form gel; wherein the mass concentration of the collagen dispersion liquid is 5-10%, and the mass ratio of the cross-linking agent to the collagen is 5-10%; the total weight percentage of the collagen and the cross-linking agent is 5.5 to 10.5 percent. The gel with a stable structure can be effectively formed when the content of the cross-linking agent is within the content range, the residual amount of the cross-linking agent is low, and the storage modulus, the swelling degree and the pushing force of the gel are excellent.

The invention provides III-type recombinant collagen, belongs to the technical field of biology, and comprises an amino acid sequence of the III-type recombinant collagen as shown in SEQ ID NO.1. The invention also provides a preparation method of the III-type recombinant collagen. According to the technical scheme provided by the invention, the purification process is simplified, the production cost is reduced, and the complex recombinant collagen repair gel provided by the invention can repair the skin barrier and relieve dry itching and stabbing pain of the skin.

The invention relates to injectable cross-linked collagen gel and a preparation method thereof. Two crosslinking strategies of self-crosslinking and crosslinking agent crosslinking are fused together for the first time, and two types of amido bonds, namely amido bonds of a collagen-collagen structure (A) and amido bonds of a collagen-fatty amine-collagen structure (B), are respectively constructed through two crosslinking paths, namely catalysis of collagen polypeptide self-crosslinking and catalysis of fatty amine crosslinking, so that a hard / soft dual crosslinking network is obtained. The relative proportion of the structure A and the structure B in the final product is adjusted through a proper cross-linking process, so that the strength and the stability of the final product are adjusted and controlled, and the use requirements of different injection parts can be met.

Systems, methods, and devices for internal wound treatment include a hemostasis device. The hemostasis device has an insertion tube extending from a base portion. One or more openings are formed into a surface of the insertion tube. Additionally, the hemostasis device includes an inflation pump couplable to a proximal of the insertion tube for pumping air into an inner channel of the insertion tube. A wound channel balloon is disposed at least partially around the insertion tube such that an actuation of the inflation pump causes the air to flow from the inner channel through the one or more openings and inflate the wound channel balloon. The wound channel balloon can include a grooved elastic fiber, one or more bulges, and / or one or more branches. Furthermore, a collagen gel, an antibiotic, a blood clotting agent, an analgesic, stem cells, a sealant, and / or a lubricant can be disposed on the wound channel balloon.

The invention discloses a collagen implant containing polynucleotide as well as a preparation method and application thereof, and relates to the technical field of implants. The collagen implant containing polynucleotide is in a gel state and is formed by mixing polynucleotide gel and collagen gel, the content of collagen in the implant is 25 to 40 mg / mL, and the content of polynucleotide in the implant is 5 to 20 mg / mL. The implant formed by collagen and polynucleotide in a proper ratio has high viscosity, can play a good anti-wrinkle effect, and is good in stability and not prone to bleeding and layering.

The invention discloses an anti-aging three-dimensional high-uniformity dermis evaluation model based on image analysis, and belongs to the technical field of biological medicine and cosmetic efficacy evaluation. The method comprises the following steps: mixing a prepared fibroblast suspension and a neutralized collagen solution at 0-5 DEG C, inoculating the mixture into a 24-pore plate in a 100-200L / pore manner, standing in an incubator at 37 DEG C for 20-40 minutes to form collagen gel, carrying out suspension culture to obtain a 3D dermal model, and detecting the shrinkage rate of the model and evaluating the anti-aging effect in combination with a high-content cell imaging system. The method overcomes the defect that a traditional method is large in error, an efficient and accurate evaluation system is provided for anti-aging drug screening, and the method has application value in cosmetic effect detection and skin aging mechanism research.

1.The name of the design product: packaging box (Wei Mei Hive Col 164.88° vertical box three). 2.The use of the design product: packaging box for injection of recombinant humanized type III collagen gel. 3.The design points of the design product: the combination of shape, pattern and color. 4.The picture or photo that best indicates the design points: perspective view. 5.The design for which protection is sought contains color.

This invention relates to the field of biomedical materials technology and discloses a recombinant collagen gel and its preparation method. The preparation method is as follows: recombinant collagen is dissolved in glacial acetic acid solution and stirred until completely dissolved to obtain a recombinant collagen solution. The recombinant collagen solution is added to phosphate buffer, and then modified chitosan, phenylboronic acid-modified sodium alginate, and glycerol are added. The mixture is stirred for 30-40 minutes to obtain a gel precursor solution. An aldehyde-modified epoxy-thiol polymer is dissolved in dimethyl sulfoxide to prepare a stock solution. The stock solution is then added dropwise to the gel precursor solution, and the mixture is stirred for another 5-10 minutes. The mixture is injected into a sterile mold, allowed to stand for 12-24 hours, defoamed, sterilized, and then sealed and stored at 4°C to obtain the recombinant collagen gel. The recombinant collagen gel prepared by this invention has good mechanical properties, self-healing properties, is safe and non-toxic, and has high biocompatibility.

The invention relates to the technical field of biological medicine, in particular to a collagen gel delivery system integrated with polypeptide medicine and a preparation method and application thereof.The collagen gel delivery system is characterized in that collagen serves as a basic gel matrix, covalent cross-linking is conducted through 4-arm-PEG-NHS, then active polypeptide is connected into a gel network through amido bonds, and the collagen gel delivery system integrated with the polypeptide medicine is obtained. The active polypeptide is an antibacterial peptide and / or an MMP-9 cyclopeptide inhibitor. The collagen gel delivery system is expected to realize the efficient and ordered healing promotion effect on the wound infected by diabetes mellitus through a multi-target synergistic mechanism such as anti-infection, anti-inflammation, collagen degradation inhibition and collagen synthesis promotion, and provides a new idea and a new option for clinical response.

The application provides a recombinant type III humanized collagen gel based on macromolecular crowding effect and a preparation method and application thereof. First, an rhCol III solution system is prepared; then a macromolecular crowding bath containing dextran 70, polyethylene glycol 8000 and ascorbic acid is prepared; then the rhCol III solution is added into the macromolecular crowding bath to form rhCol III microgel; finally, a 3D printing molecular exclusion mold system with agarose as a base, containing functional additives and a temperature-sensitive polymer as a temperature-sensitive release and space-restricted component is constructed to realize directional construction of the rhCol III microgel. The application realizes physical gelation of the rhCol in seconds (<15 s) and constructs a physiological microenvironment supporting cell proliferation. The recombinant type III humanized collagen gel prepared by the application can be used for preparing collagen microgel balls, collagen bundles, 3D biological printing and other products, and has a wide range of applications.

The utility model discloses a recombinant collagen gel stirring device, relates to the technical field of recombinant collagen gel stirring, and solves the problems that due to the specific properties of gel, the interaction between molecules of the gel is relatively strong, a completely uniform state is possibly difficult to realize in the stirring process, and even the concentration of a part of regions is too high or too low, and the like. Comprising a tank body and a supporting mechanism, a stirring mechanism comprises a second servo motor and a rotating rod, an annular rod is fixedly installed on the outer side of the top of the rotating rod, and a first blade and a second blade are fixedly installed on the outer side of the rotating rod and the inner side of the annular rod respectively. Through the stirring movement of the first blade and the second blade which are respectively mounted on the outer side of the rotating rod and the inner side of the annular rod, and the unique design of the second blade stirs the raw materials deposited on the bottom wall of the tank body to lift upwards, so that the uniform state which is difficult to achieve in the stirring process is realized, and the problems of too high or too low concentration of partial regions and the like are avoided.

This invention belongs to the field of biomedical technology, specifically relating to a high-concentration collagen-based hydrogel, its preparation method, and its applications. The high-concentration collagen-based hydrogel provided by this invention is obtained by first modifying natural collagen with a carboxylating agent, and then adding a carbodiimide crosslinking agent for crosslinking. The beneficial effects of this invention are: modifying natural collagen with a carboxylating agent improves its solubility, increasing its solubility in neutral and alkaline aqueous solutions; simultaneously, crosslinking the carboxylated collagen with a carbodiimide crosslinking agent achieves the construction of a carboxylated collagen spatial network. The resulting collagen-based hydrogel is colorless and transparent, possessing a dry matter content and mechanical properties far exceeding those of natural collagen gels, and can be applied in fields such as bio-3D printing, in-situ filling, drug loading, and delivery.

The invention provides an irradiation crosslinking collagen injection filler as well as a preparation method and application thereof. The collagen injection filler comprises collagen gel particles, and the collagen gel particles are of a porous structure. The collagen gel particles have connected fiber structures, the fiber structures are formed by collagen self-assembly, and the fiber structures comprise fiber bundles and / or fiber mesh sheet structures; the collagen gel particles in the collagen injection filler have fiber structures formed after collagen self-assembly, that is, collagen is spontaneously assembled from collagen molecules to fiber bundles and fiber mesh lamellar structures, the mechanical property of a fiber network is improved, and the degradation rate is delayed. The fiber network structure provided by the invention has good mechanical support performance and slow degradation rate, simulates a three-dimensional microenvironment of a natural extracellular matrix (ECM), and promotes cell attachment proliferation and tissue regeneration. The fiber structure is beneficial to creeping proliferation of cells and promotes regeneration and repair of skin cells.

1. The name of the design product: packaging box (Wei Mei Hive Col 164.88° horizontal box). 2. The use of the design product: packaging box for recombinant humanized collagen type III gel for injection. 3. The design points of the design product: the combination of shape, pattern and color. 4. The picture or photo that best indicates the design points: perspective view. 5. The design claimed contains color.

The invention relates to the technical field of food processing machinery, and discloses an instant fish maw processing, producing and filtering device which comprises a soaking pool, a flexible filtering inner container is transversely arranged in the soaking pool and used for containing fish maw, and the two ends of the flexible filtering inner container are fixedly connected with a feeding pipe and a discharging pipe respectively. Pipe bodies of the feeding pipe and the discharging pipe penetrate through and are fixedly connected to the side walls of the two ends of the soaking pool respectively, a peristaltic wave driving unit and a fluid replacement unit are arranged outside the flexible filtering inner container, the peristaltic wave driving unit is used for generating peristaltic waves propagating in the axial direction on the flexible filtering inner container, and the fluid replacement unit is used for replacing the peristaltic waves on the flexible filtering inner container. And the fluid replacement unit is used for executing a water inlet action at the creeping wave peak position and executing a pollution discharge action at the creeping wave valley position. The flexible filtering inner container serves as a bearing interface, and programmed flexible rubbing force is generated through the peristaltic wave driving unit. And the force is uniformly and controllably acted on the surface of the fish maw, so that the damage of high shearing force to a delicate collagen gel structure after soaking is avoided.

The invention relates to a bionic collagen matrix with a density gradient double-layer structure, and belongs to the technical field of medical materials. The collagen matrix takes telopeptide collagen derived from mammalian dermis or achilles tendon as a main component, and comprises a double-layer structure of a compact layer and a loose layer, and the compact layer is smooth in surface and compact in structure, so that cell migration can be blocked, and a certain protection effect is achieved; the loose layer is of a porous structure, cell ingrowth and blood clot stabilization are facilitated, and a support is provided for tissue repair. The preparation method of the biological membrane comprises the following steps: preparing telopeptide-removed collagen gel, preparing collagen sponge, preparing a collagen tissue regeneration membrane, and compounding the collagen tissue regeneration membrane and the collagen sponge into an integrated double-layer structure through bonding, drying and crosslinking. The double-layer structure simultaneously meets the double requirements of barrier protection and tissue regeneration, the process is simple and controllable, and the double-layer structure is suitable for clinical scenes such as oral soft tissue repair, alveolar ridge reconstruction and tooth extraction pit coverage.

This invention proposes a method for determining the glycerol content in collagen colloids. It utilizes the oxidation of free glycerol by periodic acid to formaldehyde, which then undergoes a Hantzsch reaction with two molecules of acetylacetone in the presence of ammonium acetate. This is the first time this method has been applied to the determination of glycerol content in collagen colloids, and it is automated using an automated analyzer. This method can be used to detect collagen colloidal materials. The sample pretreatment primarily utilizes the isoelectric point precipitation property of collagen. The pH of the collagen solution sample is pre-adjusted to approximately 5.0, close to the isoelectric point of collagen colloids. The precipitation of proteins clarifies the solution and facilitates the release of glycerol into the solution, resulting in more accurate measurement results. This invention offers advantages such as simplicity, speed, accuracy, and safety in determining the glycerol content in collagen colloids.

The invention relates to the technical field of collagen gel, in particular to a dialdehyde functional cross-linking agent, a preparation method of the dialdehyde functional cross-linking agent, collagen antibacterial hydrogel prepared by applying the dialdehyde functional cross-linking agent and application of the collagen antibacterial hydrogel. The dialdehyde functional cross-linking agent disclosed by the invention not only can be used as a cross-linking point of collagen to construct a stable three-dimensional network, but also can retain an antibacterial functional group of vanillin, so that successful preparation of the collagen antibacterial hydrogel is realized. The vanillin is directly converted into the cross-linking agent, so that the drug resistance risk of antibiotics is avoided, and the two advantages of tissue regeneration of collagen and natural antibacterial property of vanillin are organically integrated; the prepared collagen antibacterial hydrogel is expected to provide new treatment options for chronic difficult-to-heal wounds (such as diabetic foot ulcers, venous ulcers or pressure sores), burn wounds, surgical wound hemostasis and other easily-infected scenes, and remarkable economic benefits and social benefits are generated.

The utility model discloses a collagen protein gel filtration device relates to the field of gel filtration, including filter vat, be provided with vibration type filter assembly on the filter vat, and vibration type filter assembly includes filter screen plate, vibration top plate and vertical connecting rod, and filter screen plate is arranged in the filter vat, and the outer wall of filter screen plate contacts the inner wall of filter vat, and vibration top plate sets up the top of filter vat, and filter screen plate is connected vibration top plate through a plurality of vertical connecting rods, and a plurality of vertical connecting rods are distributed along the circumferential direction of filter screen plate, and the spring is set up on vertical connecting rod, and one end of spring is connected vibration top plate, and the other end is connected in the top of filter vat, and vibration top plate has the freedom of reciprocating motion along the vertical direction, and the reciprocating motion of vibration top plate drives filter screen plate to move up and down and produces vibration, can effectively avoid the blockage of filter screen of collagen protein gel because of viscosity adhesion, and the rate of gel through filter screen is accelerated, and the purification efficiency is improved.

This invention discloses a tissue regeneration-promoting filler material and its preparation method. The filler material is made by dispersing composite microspheres in a type I collagen gel, using type II collagen as the microsphere wall material and hydroxyapatite as the core material. The tissue regeneration-promoting filler material prepared by this invention is used for filling areas such as eyebrow wrinkles, eyebrow subcutaneous support, malar fat and buccal fat pads, tear troughs, nasolabial folds, nose, lips, chin, and mandibular periosteum. It provides significant mechanical support and, based on exogenous collagen supply, continuously stimulates the formation and rearrangement of endogenous collagen, meeting the needs of cosmetic surgery.

The invention discloses collagen gel as well as a preparation method and application thereof. The preparation method comprises the following steps: respectively degerming, mixing and cross-linking a prepared collagen solution and a prepared glutamine transaminase solution to prepare a collagen gel stock solution, and then carrying out chromatography enzyme removal or hollow fiber enzyme removal to prepare the collagen gel. According to the invention, the glutamine transaminase is used as a cross-linking agent, and residual glutamine transaminase in the collagen gel is removed through anionic membrane chromatography or hollow fibers, so that the safety problem caused by a chemical cross-linking agent and the potential safety hazard when the glutamine transaminase is used for injecting the collagen gel are avoided. Besides, by controlling the mixing ratio of the collagen and the glutamine transaminase, the physical state of the gel is regulated and controlled, and abundant raw materials are provided for biomedical materials.

1. The name of the design product: packaging box (Wei Mei Hive Col 164.88° vertical box two). 2. The use of the design product: packaging box for recombinant humanized collagen gel for injection. 3. The design points of the design product: the combination of shape, pattern and color. 4. The picture or photo that best indicates the design points: perspective view. 5. The design protected contains color.

The invention relates to the technical field of biological medicine, and discloses an exosome preparation for treating thin endometrium and a preparation method thereof, and the preparation method comprises the following steps: step 1, culturing mesenchymal stem cells; step 2, extracting an exosome; step 3, preparing a collagen solution; step 4, preparing traditional Chinese medicine components; and step 5, uniformly stirring the collagen solution and the sheep placenta powder extracting solution under the condition of 4 DEG C, then adding the exosome solution, mixing, then adding the soy isoflavone solution and auxiliary materials, fully reacting to obtain exosome gel, and storing under the condition of 4 DEG C. According to the exosome preparation for treating the thin endometrium and the preparation method of the exosome preparation, collagen gel is used as a slow-release matrix, three-dimensional structure support is provided for active substances, degradation of the active substances is slowed down, meanwhile, growth factors in the sheep fetus powder extracting solution and the exosome act together, revascularization is activated, blood supply is improved, and the effect of treating the thin endometrium is achieved. The regrowth of the endometrium is promoted.

The invention relates to a recombinant humanized I-type collagen gel and a preparation method and application thereof, and relates to the technical field of medical biomaterials, the recombinant humanized I-type collagen gel comprises a gel formed by an amido bond reaction of a recombinant humanized I-type collagen solution and multi-arm polyethylene glycol active ester, wherein the number of arms of the multi-arm polyethylene glycol active ester is four, the molecular weight of the multi-arm polyethylene glycol active ester is 5-20 kDa, and the mass ratio of the recombinant humanized I-type collagen to the multi-arm polyethylene glycol active ester is (20-50): 1. The problems that in the prior art, the crosslinking degree of recombinant humanized I-type collagen gel is uncontrollable, and the stability in the physiological environment is insufficient are solved.

1. The name of the design product: packaging box (Wei Mei Hive Col 164.88° vertical box). 2. The use of the design product: packaging box for recombinant humanized collagen type III gel for injection. 3. The design points of the design product: the combination of shape, pattern and color. 4. The picture or photo that best indicates the design points: perspective view. 5. The design protected contains color.

The invention relates to the field of medical beauty materials, in particular to injectable cross-linked recombinant collagen gel for medical beauty and a preparation method of the injectable cross-linked recombinant collagen gel. Mixing the recombinant collagen with glutamine transaminase, and carrying out cross-linking reaction to form gel; carrying out dialysis on gel formed by crosslinking in normal saline at the temperature of 2-8 DEG C for 12-24 hours so as to remove free glutamine transaminase; diluting the dialyzed gel to a target concentration; and filling the diluted gel, and carrying out terminal moist heat sterilization under the conditions that the temperature is 121 DEG C and the standard sterilization time is greater than or equal to 12 minutes, so that the gel is homogenized. According to the technical scheme, through specific crosslinking and subsequent process treatment, the obtained gel can tolerate terminal moist heat sterilization conditions that the temperature is 121 DEG C and the F0 is greater than or equal to 12 minutes. Therefore, an expensive and high-risk sterile filling process can be abandoned in production, but a terminal sterilization process with more consistent batch sterility guarantee level is adopted, so that the production cost and the quality risk are remarkably reduced.

1. The name of the design product: packaging box (Wei Mei Hive Col 164.88° white box). 2. The use of the design product: packaging box for recombinant humanized collagen type III gel for injection. 3. The design points of the design product: the combination of shape, pattern and color. 4. The picture or photo that best indicates the design points: perspective view. 5. The design claimed contains color.

The invention discloses a preparation method and application of a magnetic biomass carbon material (CaFe2O4-coated FNC), and belongs to the technical field of solid waste resourceful treatment.The preparation method avoids use of a calcium metal salt reagent, waste fish scales serve as a precursor, the fish scales contain collagen and hydroxyapatite, the collagen in the fish scales can be fully extracted through alkali treatment, and the magnetic biomass carbon material (CaFe2O4-coated FNC) is obtained. Forming an ichthine extracting solution (which is a collagen gel solution); residual filter residues are mainly hydroxyapatite and can be used as a high-efficiency phosphate fertilizer, high-value utilization of fish scales is realized, and the prepared magnetic biomass carbon material is a nitrogen-rich doped porous carbon material modified by nano-CaFe2O4, can effectively adsorb antibiotics and can be magnetically separated and recycled at the same time.