Collagen gel artificial skin substitute

A collagen gel and dermis technology, applied in the field of bioengineering, can solve the problems of no such products on the market or apply for patents, and achieve the effect of promoting the migration of adjacent epithelium, promoting the formation of granulation tissue, and promoting repair and regeneration

Inactive Publication Date: 2004-07-21
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] There is no such product on the market or patent application in China; there are similar products abroad, but no artificial leather with the same composition is on the market or patent application

Method used

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  • Collagen gel artificial skin substitute
  • Collagen gel artificial skin substitute

Examples

Experimental program
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example 1

[0026] Take human fetal dermal fibroblasts of the 2nd-3rd generation in the exponential growth phase, digest with 0.25% trypsin solution, pipette, centrifuge, suspend in DMEM-10% fetal bovine serum culture medium, count on a hemocytometer, and adjust the cell concentration to 5.0×10 6 / ml, stand-by; prepare 0.2% collagen dispersion with 0.01M acetic acid solution (sterilized) and bovine tendon type I collagen (sterilized) extracted in this laboratory, take 40ml for stand-by; prepare concentrated DMEM (10 times) culture solution 5ml, 0.3M glutamine solution 0.5ml, 1M sodium bicarbonate solution 1.8ml, fetal bovine serum 6ml; the above 4 kinds of solutions and 40ml collagen dispersion were dropped into 100ml sterile cone Add 3ml of fibroblast suspension to a shaped bottle (placed on ice, that is, under ice bath conditions), mix quickly, and immediately drop the collagen-cell suspension into a 12-well plate with a pipette, each well 3ml, 37°C incubator for 3-5 minutes, after the...

example 2

[0037] Take human fetal dermal fibroblasts of the 2nd-3rd generation in the exponential growth phase, digest with 0.25% trypsin solution for 0.5-1 minute, add 2ml of fetal bovine serum to stop the digestion, pipette, disperse the cells into a single-cell suspension, and suspend In DMEM-10% fetal calf serum culture medium, count on a hemocytometer and adjust the cell concentration to 5.0×10 6 / ml, stand-by; prepare 0.2% collagen dispersion with 0.01M acetic acid solution (sterilized) and bovine tendon type I collagen (sterilized) extracted in this laboratory, take 80ml for stand-by; prepare concentrated DMEM (×10) 10ml of culture medium, 1ml of 0.3M glutamine solution, 3.6ml of 1M sodium bicarbonate solution, 12ml of fetal bovine serum; drop the above 4 kinds of solutions and 80ml of collagen dispersion into a 200ml sterile conical Add 6ml of human fetal dermal fibroblast suspension to the bottle (placed on ice), mix quickly, and immediately drop the collagen-cell suspension in...

example 3

[0039] Take human fetal dermal fibroblasts of the 2nd-3rd generation in the exponential growth phase, digest with 0.25% trypsin solution, pipette into a single cell suspension, suspend in DMEM-10% fetal bovine serum culture medium, and count with a hemocytometer , adjust the cell concentration to 5.0 × 107 / ml, stand-by; use 0.01M acetic acid solution (sterilized) and bovine tendon type I collagen (sterilized) extracted in this laboratory to prepare 0.2% collagen dispersion, Get 20ml for later use; prepare 2.5ml of concentrated DMEM (×10) culture solution, 0.25ml of 0.3M glutamine solution, 1ml of 8.4% sodium bicarbonate solution, 3ml of fetal bovine serum; mix the above 4 kinds of solutions with 40ml of collagen The dispersion was dropped into 50ml sterile Erlenmeyer flasks (placed on ice cubes) in order, and then 1ml of human fetal dermal fibroblast suspension was added, mixed quickly, and the collagen-cell suspension was immediately dropped into In a 24-well culture plate, 1...

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Abstract

An artificial collagen gel skin is prepared from ox tendon collagen dispersing liquid 80 portions, DMEM culture liquid 10, glutamine solution 1, dicarbonate solution 6 and fetal calf serum 12 through proportionally mixing, adding human embryo skin fibroblast suspension, and external culture.

Description

technical field [0001] The invention relates to bioengineering technology, in particular to collagen gel artificial dermis and a preparation method thereof. Background technique [0002] There is no such product on the market or patent application in China; there are similar products abroad, but no artificial leather with the same composition is on the market or patent application. The technologies closely related to the present invention are collagen sponge artificial dermis, decellularized allogeneic dermis, and artificial material dermis substitutes. Collagen sponge artificial dermis requires cross-linking agents, which are toxic to the body after transplantation; acellular allogeneic dermis and artificial material dermis substitutes still have a certain gap in structure and composition from normal skin. Contents of the invention [0003] The object of the present invention is to provide a collagen gel artificial dermis and a preparation method thereof. The invention ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60
Inventor 张其清马东瑞
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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