205 results about "Skin fibroblast" patented technology

Methods and cell culture medium for the generation of human pluripotent embryonic stem cells are disclosed. Human embryonic stem cells are cultured with human granulosa feeder cells, muscle cells, Fallopian ductal epithelial cells, bone marrow stromal cells, and skin fibroblasts and the embryonic stem cells maintain their pluripotent phenotype. The human pluripotent embryonic stem cells can be cultured without feeder cells, and in the presence of supplemental growth factors. The human pluripotent embryonic stem cells can be alternatively cultured with conditioned medium obtained from a cell culture capable of maintaining human embryonic stem cells in a pluripotent state, wherein the cell culture is a human granulosa cell culture.

The invention discloses a giant salamander active peptide and an application. The giant salamander active peptide is prepared by the following steps: conducting enzymatic hydrolysis on giant salamander meat which serves as a raw material by virtue of a complex enzyme of marine alkaline protease and papain; separating an enzymatic hydrolysate by virtue of a trypsin immobilized ultra-filtration membrane separator; and then conducting separation and purification by virtue of Sephadex LH-20 molecular sieve chromatography and high performance liquid chromatography, so that the giant salamander active peptide is obtained. The giant salamander active peptide is capable of effectively scavenging free radicals and boosting body immunity, and meanwhile, the active peptide can also promote proliferation of skin fibroblasts; therefore, the giant salamander active peptide has a broad application prospect in the fields of food, medicines and cosmetics.

The invention is directed to compositions containing growth agents synthesized from cultured cells from skin. Skin cells such as keratinocytes and dermal fibroblasts are cultured in vitro in cell medium and in the course of culture the cultured cells synthesize and secrete agents into the cell medium. The medium containing agents are collected and incorporated into pharmaceutical or cosmetic preparations to treat an individual. The preparation is applied and has a rejuvenating effect on the cells and tissue.

The invention provides a herbal composition and an application thereof; the composition includes radix asparagi and rhizoma rehmanniae or also includes ginseng; the composition can improve the activity of superoxide dismutase SOD and hyaluronic acid synthase HAS3 in skin fibroblasts by promoting proliferation of the skin fibroblasts so as to achieve good biological-activity antioxidant anti-aging and moisturizing effects, and can achieve antioxidant anti-aging and moisturizing double effects when added to cosmetics and/or skin care products.

A tissue-engineered epidermis is prepared from skin fibroblasts through obtaining the skin fibroblasts, preparing culture medium, amplifying culture, preparing extocytic matrix compound, preparing biologic scaffold, compounding the skin fibroblasts on the surface of biologic scaffold, and 3D culture. Its advantages are a certain elasticity and toughness, short culture time, and no obvious immunorejection reaction.

The invention belongs to the biotechnology filed and relates to a pigmented skin equivalent used for screening decolorizing agents and a constructing method thereof. A dermic skin equivalent layer is constructed by skin fibroblasts that are cultured in vitro and collagen, and the equivalent is constructed after inoculating keratinocyte and melanocyte on the skin equivalent layer by using gas-liquid plane three-dimensional culture technology. The obtained pigmented skin equivalent that is similar to the structure of skin can imitate the transportation process of melanin bodies, be used for screening the decolorizing agents and provide a more scientific model for the study of melanocyte in vitro biology and the screening of decolorizing agents and whitening cosmetics.

The present invention includes peptides, compositions as well as their use for the prevention or treatment of various age related or pathological conditions of skin or other tissues including skin wrinkles, wounds, different types of fibrosis and methods of reconstructing different tissues such as techniques used in regenerative medicine. The invention further includes peptide mimetics and methods of use including interfering with transcriptional complexes characteristic of fibroblasts of aged skin and stimulating synthesis of structural components of extracellular matrix.

The disclosed invention provides tetrapeptides with the amino acid sequence proline-glutamine-glutamate-X (P-Q-E-X), where X can be either lysine (K) or isoleucine (I). These tetrapeptides inhibit ultraviolet light (UV)-induced expression of the pro-inflammatory cytokine interleukin-6 (IL-6) by skin epithelial cells and fibroblasts. Furthermore, the tetrapeptides repress the upregulation of matrix metalloproteinase-1 (MMP-1) by skin fibroblasts induced by either direct exposure to UV rays or treatment with media conditioned by UV-treated keratinocytes. The small size and bio-activity of the tetrapeptides render them suitable for use in therapies directed to inflammatory skin disorders and as active ingredients in skin care products.

The invention discloses an Rg2 group and an Rh1 group of red ginseng saponin and a preparation method as well as applications in preparing cosmetics preventing skin aging. Extracts which are extracted from ginseng roots, do not contain saponin, and contain saponin enzyme of ginseng and other water-soluble matters react with ginsenoside Re to obtain red ginseng mixed saponin Rg2 group consisting of red ginseng rare saponin 20(S)-Rg2, 20(R)-Rg2, Rg4 and Rg6, and the extracts react with ginsenoside Rg1 to obtain the red ginseng mixed saponin Rh1 group consisting of red ginseng rare saponin 20(S)-Rh1, 20(R)-Rh1, Rh4 and Rk3. Compared with a saponin Rg2 monomer and a saponin Rh1 monomer, the function of the Rg2 group and Rh1 group of red ginseng mixed saponin for promoting skin fibroblast collagen synthesis is improved obviously, the Rg2 group and Rh1 group of red ginseng mixed saponin are more safe to skin and human bodies, and have less side effects.

A tissue-engineered skin containing vascular structure features that it contains skin fibroblasts, keratinous cells and vascular endothelial cells. Its configuring method includes separating said three kinds of cells from eath other, external culture, proportionally mixing, and configuring the artificial skin with vascular structure. Its advantages are high survival rate and high resistance to infection.

The increasing use of nanotechnology in consumer products and medical applications underlies the importance of understanding its potential toxic effects to people and the environment. Herein are described methods and assays to predict and evaluate the cellular effects of nanomaterial exposure. Exposing cells to nanomaterials at cytotoxic doses induces cell cycle arrest and increases apoptosis/necrosis, activates genes involved in cellular transport, metabolism, cell cycle regulation, and stress response. Certain nanomaterials induce genes indicative of a strong immune and inflammatory response within skin fibroblasts. Furthermore, the described multiwall carbon nanoonions (MWCNOs) can be used as a therapeutic in the treatment of cancer due to its cytotoxicity.

An artificial collagen gel skin is prepared from ox tendon collagen dispersing liquid 80 portions, DMEM culture liquid 10, glutamine solution 1, dicarbonate solution 6 and fetal calf serum 12 through proportionally mixing, adding human embryo skin fibroblast suspension, and external culture.

The invention discloses an umbilical cord mesenchymal stem cell preparation and a preparation method and application thereof. Through exploration and verification by a large quantity of experiments, the 3rd-10th-generation umbilical cord mesenchymal stem cells are selected as raw materials, and by a method of dispersing tissue by a mechanical method and then performing treatment with mixed enzymes containing collagenase I, collagenase II and trypsin for a short time for many times, the vitality of the separated umbilical cord mesenchymal stem cells is guaranteed. A culture medium special for the umbilical cord mesenchymal stem cell preparation, containing DMEM/F12 (without phenol red), 10% FBS, 20 kinds of amino acids and 8 kinds of vitamins, is adopted, mixed liquor of repeated cell culture supernatant and cell lysate supernatant is collected, more umbilical cord mesenchymal stem cell active protein and active factors are obtained, the preparation cost is reduced, and industrial requirements for a stem cell product preparation are met. The prepared umbilical cord mesenchymal stem cell preparation can promote proliferation of epidermic cells and skin fibroblast, is safe and is free from toxic and side effects, an allergy does not exist, and when the umbilical cord mesenchymal stem cell preparation is applied to feature-beautifying skin-care products, the effects are notable.

Methods for preventing, ameliorating, or reducing dermatological signs of aging are provided which employ active agents that suppress or down-regulate microRNA expression in dermal fibroblast, resulting in enhanced production of collagen, elastin and/or fibrillin in the skin. Also provided are methods for screening for activity against specific microRNAs and the methods of using active agents identified by the screening protocol in the treatment of skin.

The invention provides a method for inducing human skin fibroblasts to differentiate into adipocytes in vitro. The method comprises the following steps: A, carrying out in-vitro culture and passage onthe human skin fibroblasts in a basal culture medium, and carrying out induced differentiation when the cell confluence degree reaches 80-100%; and B, changing the basic culture medium in the step Ainto an induced differentiation culture medium (1), culturing for 6-10 days, and changing liquid once every two days; then, replacing the induced differentiation culture medium (1) with an induced differentiation culture medium (2), and culturing for 2-4 days; and finally, changing the induced differentiation culture medium (2) into a basic culture medium, culturing for 8-14 days, and changing liquid once every two days. According to the method, the problem that human skin fibroblasts are induced to be differentiated into adipocytes is successfully solved; the skin fibroblasts are induced to be differentiated into the adipocytes, so that scars left in skin during wound healing are reduced, and a new thought and a new therapy are provided for clinical wound repair, scar repair and the like.

The invention discloses a method for obtaining autologous skin fibroblasts, PRP and collagen liquid, which comprises the following steps: separating, culturing and passing down skin fibroblasts; preparing autologous high-concentration collagen-based secreted concentrated protein liquid ; Preparation of autologous platelet-rich plasma. The present invention is designed and prepared for the main reasons of skin aging and scar formation. The three components of the preparation—autologous skin fibroblasts, autologous growth factor PRP, and autologous collagen—guarantee the effectiveness of the preparation and keep it for a long time. Features; and all three components are autologous, which ensures the safety of the preparation, which is better than the effect of simply using autologous fibroblast transplantation on the market. The main content of the present invention is a method for preparing the supernatant concentrate mainly composed of secreted collagen.

The invention relates to a proliferation accelerant and application thereof, in particular to a fibroblast proliferation accelerant capable of promoting fibroblast proliferation and fibroblast extractand fibroblast extract freeze-dried powder prepared through fibroblasts. The proliferation accelerant comprises loofah saponin and/or momordica saponin. A loofah saponin and momordica saponin combined inducer is adopted for culture of skin fibroblasts, so that synthesis and secretion of bioactive substances derived from the skin fibroblasts are promoted effectively, content of active ingredientsin cell derivatives is increased, and production efficiency and biologic effect of the skin fibroblast derived cell derivatives.

The invention provides a carbohydrate composition with a wound healing promoting effect and application of the carbohydrate composition. The carbohydrate composition contains at least five types of carbohydrate including carboxymethyl chitosan, rhizoma bletillae polysaccharides, fucoidan from fucus vesiculosus, heparan sulfate, chondroitin sulfate, oligosaccharides of hyaluronan and gulose aldehyde acid oligosaccharides. The carbohydrate composition and the application have the advantages that VEGFA (vascular endothelial growth factor A), FGF2 (fibroblast growth factor 2) and VE-cadherin protein mRNA (messenger ribonucleic acid) expression can be obviously improved by the carbohydrate composition, VEGF (vascular endothelial growth factor) secretion and skin fibroblast (HSF) and human immortalized keratinocyte (HaCaT) proliferation can be obviously promoted by the carbohydrate composition, and the obvious wound healing promoting effect can be realized by the carbohydrate composition; raw materials required by the carbohydrate composition are easily available, composition methods for the carbohydrate composition are simple, the carbohydrate composition is low in cost, easy to industrialize and wide in application range, and the like.

The invention discloses a method for transfecting bovine somatic cells into inducted pluripotent stem cells (iPSCs) by adopting transcription factors, concretely comprising the following steps: 1) isolation and culture of bovine dermal fibroblasts; 2) cloning of bovine transcription factor genes maintaining pluripotency and construction of retroviral vectors of the transcription factor genes; 3) retrovirus packaging and preparation; and 4) infection of bovine dermal fibroblasts by retrovirus and acquisition of bovine inducted pluripotent stem cells. The invention first adopts the acellular human amniotic membranes as support to replace the mouse embryonic fibroblasts (MEFs) to maintain in vitro proliferation of the bovine iPSCs and keep the bovine iPSCs in undifferentiated pluripotent states for long time. The method which adopts gene inducing to generate the PSCs not only solves the technical problem that the PSCs of the animals such as cattle, sheep, pigs and other livestock are difficult to obtain but also avoids the problem of heterogeneous animal cell pollution caused by the feeder layer which applies the MEFs as the stem cells.

The invention discloses an application of a transdermal peptide modified pueraria thomsonii exosome nano preparation in preparation of an anti-skin aging product. The pueraria thomsonii exosome nano preparation modified by the transdermal peptide can effectively penetrate through the cuticle of the skin and is efficiently taken by skin cells such as skin fibroblasts, so that the obvious function of resisting skin (cell) aging is exerted, and a new strategy is provided for skin aging resistance; The transdermal peptide modified pueraria thomsonii exosome can be used as a novel skin anti-aging beauty product which is efficient, safe and convenient to prepare, can also be applied to aging resistance of other cells and tissues, and is used for delaying aging and function decline of body tissues and organs; therefore, great market development prospects are realized in the fields of medical products and beauty cosmetics.