95 results about "Skin fibroblast" patented technology

The invention relates to the technical field of biological medicine, in particular to recombinant III-type humanized collagen as well as a preparation method and application thereof. In one embodiment, the recombinant III type humanized collagen prepared by the invention has an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID NO: 4. The recombinant III-type humanized collagen prepared by the invention has good biological activity, can promote collagen synthesis related gene expression, promote skin fibroblast proliferation, increase the content of skin collagenous fibers and elastic fibers, and improve skin elasticity and compactness; in addition, the expression of osteogenesis related genes is promoted, and the proliferation of osteoblasts is promoted. The results show that the recombinant III-type humanized collagen prepared by the invention has an extremely wide application prospect in developing related medical and cosmetic products for delaying skin aging and promoting wound repair and bone tissue healing.

The invention discloses a composition for delaying cell senescence, and the composition for delaying cell senescence comprises fisetin, urolithin A and ergothioneine, or consists of fisetin, urolithin A and ergothioneine. The invention also discloses an application of the composition for delaying cell aging in preparation of a product for delaying skin fibroblast aging, and an anti-aging preparation containing the composition. According to the composition for delaying cell aging, fisetin, urolithin A and ergothioneine contained in the composition can generate a synergistic effect, aging of skin fibroblasts can be remarkably delayed, and healing and repairing of skin are promoted.

Provided is a collagen production promoter comprising, as an active ingredient, a triglyceride represented by the following formula (I), wherein R1, R2 and R3 each denotes a saturated fatty acid residue, at least one of which is a pentadecanoic acid residue. The triglyceride comprising the pentadecanoic acid promotes the production of skin fibroblasts and collagen, and can be used as a topical skin care composition and a beauty food or beverage for improving the condition of skin.

The invention discloses an application of bulbus lilii glycoside B. The application is that bulbus lilii glycoside B is used as a unique active component or one of the active components to prepare a product for preventing or / and treating skin photoaging diseases. Experimental results of the invention show that lilium brownii glycoside B can significantly improve the cell viability of UVA-induced human skin fibroblasts, can reduce the number of beta-galactosidase positive cells in the UVA-induced human skin fibroblasts and reduce lipofuscin deposition, and can significantly improve the photoaging of the UVA-induced skin fibroblasts. Meanwhile, the skin photoaging grading of the back of a C57BL / 6 mouse induced by UVA can be remarkably improved. Therefore, the bulbus lilii glycoside B is expected to be used as an active ingredient for preparing medicines or cosmetics for preventing or / and treating the skin photoaging diseases, and particularly has important significance and remarkable application value for preventing or / and treating the UVA-induced skin photoaging diseases.

The invention provides a retinyl oligopeptide derivative with anti-aging and wrinkle fading functions and application thereof, and belongs to the technical field of polypeptide derivative application, the retinyl oligopeptide derivative is obtained by connecting a carboxyl terminal of retinoic acid and an amino terminal of oligopeptide, and has a structure as shown in a formula I. In the formula I, n is 1 or 2; l is oligopeptide. The retinoic acid modified oligopeptide derivative provided by the invention has good solubility in both a water phase and an oil phase, is beneficial to product formula design in an application process, has good antioxidant activity and skin cell injury protection activity, can reduce MMP2 and TNF alpha expression increased by ROS in skin fibroblasts, and has a good application prospect. The composition has excellent activity of promoting collagen expression, and has good effects of resisting oxidation, repairing skin injury and smoothing wrinkles when being used for cosmetics or skin care products.

The invention discloses lactobacillus pentosus capable of converting various substances of dendrobium officinale to generate rich active small molecules and improving skin health, and belongs to the technical field of microorganisms. The lactobacillus pentosus (Lactobacillus pentosus) CCFM1482 obtained by screening can be used for producing various active substances by fermenting dendrobium officinale and regulating and controlling the synthesis of skin collagen factors, such as HSF cells (human skin fibroblasts), MDA (malondialdehyde), Nrf2 (nuclear factor E2 related factor 2) and HaCaT cells (human keratinocytes). Therefore, the lactobacillus pentosus CCFM1482 fermented dendrobium officinale kimura ex migo fermentation liquor has a huge application prospect in the preparation of products for preventing or treating the skin quality, and the dendrobium officinale kimura ex migo fermentation liquor can be used for preparing the products for preventing or treating the skin quality.

The invention discloses a preparation method of a three-dimensional umbilical cord mesenchymal stem cell exosome and application of the three-dimensional umbilical cord mesenchymal stem cell exosome in the fields of medicines and medical beauty. The preparation method comprises the step of amplifying umbilical cord mesenchymal stem cells (UC-MSCs) by adopting a three-dimensional culture system. Experiments show that IGF-1, EGF, VEGF, TGF-beta, PDGF, FGF and the like in the exosome subjected to three-dimensional culture are beneficial to skin repair and oxidation resistance, and the content of cell active factors for promoting collagen secretion is remarkably increased. The umbilical cord mesenchymal stem cell exosome significantly improves the fibroblast proliferation activity, and UV-induced skin fibroblast damage model construction and function verification experiments also prove that the exosome can promote the HA level recovery of damaged skin fibroblasts. The invention provides an efficient and safe biological material for tissue repair.

The present invention relates to a composition comprising plant extracts. The composition according to the present invention has a skin whitening effect by reducing the total amount of melanin and tyrosinase activity in melanocytes of the skin, promotes skin regeneration and increases skin elasticity or reduces skin wrinkles by promoting collagen synthesis and inhibiting collagenase activity in fibroblasts of the skin, has an anti-inflammatory effect or a skin soothing effect by inhibiting NO generation, increases the amount of moisture in the skin and has a moisturizing effect by promoting generation of hyaluronic acid in fibroblasts, and has an antioxidant effect by scavenging free radicals. In addition, since the composition of the present invention exhibits a broad antibacterial effect against various bacteria, the composition may be used as a cosmetic composition, a pharmaceutical composition, a skin external preparation, or a food composition.

The invention discloses a pharmaceutical composition with skin repairing, anti-inflammatory and anti-aging effects as well as a preparation method and application of the pharmaceutical composition. The pharmaceutical composition mainly comprises a stem cell exosome and a fusion polypeptide-single domain antibody conjugate, and can also comprise proline and pharmaceutically acceptable auxiliary materials. The amino acid sequence of the fusion polypeptide is as shown in SEQ ID NO.1, and the fusion polypeptide can specifically target skin cells; the single-domain antibody is an anti-IL-6 single-domain antibody, the amino acid sequence of the single-domain antibody is shown as SEQ ID NO.2, and the single-domain antibody can efficiently inhibit inflammatory factors. Experiments show that through the synergistic effect of all the components, the composition remarkably promotes proliferation of skin fibroblasts and keratinocytes, inhibits secretion of inflammatory factors such as IL-6 and TNF-alpha, promotes synthesis of collagen, effectively improves skin aging and inflammation states and has wide application prospects in the fields of skin repair, inflammation resistance and senescence resistance.

The invention discloses an exosome composition as well as a method and application thereof for promoting collagen synthesis, and particularly relates to the technical field of cosmetics, the exosome composition comprises the following components in parts by weight: 15-30 parts of human umbilical cord mesenchymal stem cell exosome, 8-15 parts of radix scutellariae extract, 2-6 parts of acetyl hexapeptide-2 and 1-4 parts of zinc gluconate, the composition can also comprise a humectant and a thickening agent. The preparation method comprises the following steps: preparing the human umbilical cord mesenchymal stem cell exosome, dissolving the radix scutellariae extract, the acetyl hexapeptide-8 and the zinc gluconate, mixing with the exosome, adding the humectant and the thickening agent, adjusting the pH value, and removing bacteria. Through the synergistic effect of all the components, the composition can effectively promote skin fibroblasts to synthesize type I collagen, has good anti-aging and anti-inflammatory effects, is high in safety and can be widely applied to preparation of anti-aging skin care products.

The invention belongs to the technical field of biological medicines, and particularly relates to application of MIM2 and derivatives thereof in preparation of products for treating tissue fibrosis. It is found that the compound MIM2 can effectively inhibit expression and synthesis of various cancers (extracellular matrix proteins such as collagen and laminin in cells). In-vitro experiments show that after MIM2 treatment, collagen and laminin expression of the cancer cells, skin fibroblasts, hepatic stellate cells and embryonic lung cells is remarkably reduced. Animal experiments further prove that the MIM2 can effectively inhibit collagen deposition in tumor tissues of tumor-bearing mice, can slow down the fibrosis process of liver fibrosis and pulmonary fibrosis mouse models, and can reduce the level of plasma hyaluronic acid at the same time. The results show that the MIM2 has the effects of inhibiting extracellular matrix deposition and delaying tissue fibrosis, and is expected to be developed into small molecular drugs for treating cancer-related fibrosis and other fibrosis diseases.

The invention relates to the technical field of biomedicine, in particular to saccharified modified I-type collagen and a preparation method and application thereof.The preparation method of the saccharified modified I-type collagen comprises the following steps that 1, freeze-dried I-type collagen is dissolved in a PBS acid solution, then the pH is adjusted to 6.5-7.5 with an alkali solution, and an I-type collagen solution is obtained; and (2) adding methylglyoxal into the I-type collagen solution, and incubating for 25-35 days in a dry normal-temperature environment in a dark place to obtain the saccharified modified I-type collagen. The saccharified modified I-type collagen prepared by the invention can effectively simulate the pathological state of diabetic skin saccharified collagen accumulation so as to promote skin fibroblasts to generate pathological changes. The saccharified modified I-type collagen can serve as an extracellular matrix, and compared with glucose modeling, the saccharified modified I-type collagen can better simulate the pathological environment of diabetic skin saccharified collagen accumulation in vitro.

The invention discloses an application of a POGLUT1 gene in reversing senescence of skin fibroblasts. According to the application, by down-regulating expression of POGLUT1 in skin fibroblasts, SA-beta-Gal activity and expression of senescence marker genes p16, p21 and p53 can be reduced, the cell apoptosis number is reduced, and the anti-inflammatory capacity of HSFs is improved, so that the effect of reversing senescence of the skin fibroblasts is achieved. The invention discloses POGLUT1 as a core regulatory gene of MSCs for reversing skin aging, and a molecular regulatory network of the POGLUT1 in a skin aging process through a Notch signal channel, and clarifies a potential molecular mechanism of MSCs for reversing skin aging. The invention provides a new molecular target for clinically treating skin aging related diseases, also provides a new theoretical basis for understanding the effect of MSCs in skin aging, and also lays an important experimental foundation for clinically developing an anti-aging treatment strategy based on MSCs or POGLUT1 targets.

This application provides a barrier-repairing composition and its use. The composition comprises panthenol, niacinamide, folic acid, and cobalamin in a mass ratio of 3-7:1-5:0.0005-0.1:0.00005-0.01. This application also provides uses of the composition and products containing the same. The composition utilizes panthenol, niacinamide, folic acid, and cobalamin in combination, where the components complement and enhance each other, achieving a synergistic effect in upregulating BMAL2 gene expression. This enhances the DNA damage repair capacity of skin fibroblasts and potentially promotes barrier repair. Furthermore, the composition provided herein can significantly reduce the expression of capsaicin receptor 1 (TRPV1) and increase the expression of involucrin, PIEZO1, and TNXB, reducing skin discomfort and enhancing skin barrier function. Furthermore, the composition provided herein can reduce the expression of inflammatory factors, demonstrating its anti-inflammatory effect.

The present application relates to a CRISPR-mediated immortalized sheep fetal skin fibroblast cell line in the field of variation or genetic engineering and application thereof.The construction of the CRISPR-mediated immortalized sheep fetal skin fibroblast cell line of the present application comprises the following steps: step one, constructing a Cas9 site-directed knockout plasmid containing an FGF5 gene target sequence; step two, constructing a homologous repair plasmid Donor-2 kb HA-SV40LT containing an FGF5 gene homologous arm; step three, constructing a donor DNA containing an SV40LT protein expression frame; and step four, preparing a sheep fetal fibroblast cell line stably expressing SV40LT protein.The immortalized SFF-FGF5 / SV40LT monoclonal cell strain created by the present application can become an ideal tool cell line for genome editing researches such as sheep CRISPR library screening, multiple gene editing and mutation gene function exploration.

The application relates to a recombinant type I collagen and application thereof, which utilizes the characteristics of Pichia pastoris capable of secreting and expressing recombinant proteins, secretes the recombinant type I collagen on the culture medium supernatant, and reduces the difficulty of protein purification. In addition, the integrin site RGD is inserted into the collagen to form a recombinant type I collagen with high stability and high expression. According to the relative proliferation of cells and the relative adhesion experiment of cells, it can be seen that the recombinant type I collagen has the effects of promoting the proliferation of skin fibroblasts, improving the skin, promoting wound healing, promoting the adhesion of fibroblasts in the skin layer and the like. Therefore, the recombinant type I collagen has great potential applications in the fields of skin care and beauty, medical treatment and the like.

The invention discloses an application of a sesquiterpene lactone compound in preparation of a medicine for enhancing a radiation therapy effect, and belongs to the technical field of biomedicine.The elephantopus tomentosus monomer EM-2 in a formula (I) enhances G2 / M phase retardation caused by ionizing radiation by inhibiting a signal channel caused by the ionizing radiation, so that the sensitivity of the radiation therapy is remarkably improved. Experimental results show that the compound combined with radiotherapy can reduce the expression of a cell proliferation marker Ki67, reverse ionizing radiation induced p-AKT activation, and further enhance the anti-tumor effect of radiotherapy. The compound has a good proliferation inhibition effect on cervical cancer cells, and has low toxicity on normal human skin fibroblast HSF cells. On the other hand, EM-2 can be combined with AKT protein in a targeted mode to inhibit a PI3K / AKT signal transduction pathway activated by radiotherapy, meanwhile, protective autophagy activated after radiotherapy is inhibited, and the sensitivity of ionizing radiation to cervical cancer cells is improved.

The utility model discloses a skin fibroblast culture bottle which comprises a bottle body, a bottle neck is integrally formed at one end of the bottle body, a ventilation structure is in threaded connection with the outer side of one end of the bottle neck, a clamping seat is integrally formed at the top end of the bottle body, a clamping ring is integrally formed at the bottom end of the bottle body, and a culture structure is arranged on the outer side of the bottle body. A marking structure is installed on one side of the bottle body. According to the cell culture bottle, the rotatable ventilation valve is mounted at one end of the bottle cap, and the bottle cap and the ventilation valve are provided with the ventilation holes, so that air inside and outside the cell culture bottle can be exchanged when the ventilation holes in the bottle cap and the ventilation valve are overlapped, and air exchange can be stopped when the ventilation holes in the bottle cap and the ventilation valve are staggered by rotating the ventilation valve; and the polytetrafluoroethylene filter membrane in the bottle cap can ensure the entry of carbon dioxide and the discharge of oxygen and prevent microorganisms and pollutants from entering, so that the aim that the skin fibroblast culture bottle can ensure the air permeability of a growth environment is fulfilled.

The application provides a culture medium for producing dendrobium stem cell exovesicles and an application thereof, relates to the technical field of biology, and the culture medium for producing dendrobium stem cell exovesicles comprises 0.1-0.5 mg / L naphthalene acetic acid, 0.05-0.2 mg / L thidiazuron, 0.5-5 muM sodium selenate and 1-10 mg / L glutathione. The culture medium for producing dendrobium stem cell exovesicles and the method for preparing dendrobium stem cell exovesicles by using the culture medium for producing dendrobium stem cell exovesicles provided by the application have made significant progress in the yield and quality of dendrobium stem cell exovesicles. Meanwhile, the prepared dendrobium stem cell exovesicles have significant DPPH free radical scavenging capacity and improve the healing capacity of skin fibroblasts.

This invention provides a polypeptide and its application in the preparation of products that promote skin wound repair. The polypeptide of this invention, with the amino acid sequence shown in SEQ ID NO:1, not only possesses excellent anti-inflammatory and antioxidant activities, making it suitable for the preparation of anti-inflammatory and antioxidant products, but also effectively promotes the migration of skin fibroblasts and accelerates wound healing, making it suitable for the preparation of products that promote skin wound repair.

The application belongs to the technical field of antibodies, and particularly relates to an antibody for specifically treating orf of milk goat kids and application thereof. Virus liquid of a diseased goat is collected from a lesion site, and ORFV virus strains are obtained through subculture and amplification of goat skin fibroblasts; milk goats are immunized with a weak vaccine to prepare high-titer immune serum; flow sorting technology is used to separate ORFV-specific B cells from PBMC (peripheral blood mononuclear cells); antibody genes are amplified through nested PCR, expression vectors are constructed, and 293T cells are transfected to express monoclonal antibodies. The monoclonal antibody No. 2 obtained in the application has high affinity and specificity, the heavy chain CDR1-3 region sequence is SEQ ID NO: 1-3, and the light chain CDR1-3 region sequence is SEQ ID NO: 4-6. The antibody can be used for diagnosis, treatment and prevention of orf of goats, and has the characteristics of high efficiency, specificity and stability, and provides a new biological preparation for prevention and control of orf of goats.

This invention provides skin cell fibroblast- and blood-based methods for determining whether a human subject has a gene expression profile characteristic of AD. This invention also provides related methods for determining whether a demented human subject is afflicted with AD or non-ADD, and for determining whether a non-demented human subject has an increased likelihood of becoming afflicted with AD.

The present invention relates to a cosmetic composition for promoting skin regeneration, and more particularly, to a cosmetic composition for promoting skin regeneration by further comprising a polydeoxyribonucleotide (PDRN) in a composition comprising a sponge microneedle, a lactic acid bacteria exosome, and a ceramide in a specific weight ratio, and more particularly, to a cosmetic composition for promoting skin regeneration by adding a polydeoxyribonucleotide (PDRN) to a composition comprising a sponge microneedle, a lactic acid bacteria exosome, and a ceramide in a specific weight ratio. The sponge microneedle forms a microchannel in a skin cuticle to promote transmission of effective components, the lactic acid bacteria exosome induces cell viability through a cell signal transduction function, and the ceramide strengthens a skin barrier. By adding PDRN in the range of 0.001 to 3% by weight relative to the total weight of the composition, proliferation of skin fibroblasts, collagen synthesis and wound healing can be promoted. The composition can be prepared into various dosage forms such as face cream, emulsion, essence, ampoules or facial masks, and the improved skin regeneration effect is provided by simultaneously achieving the effects of strengthening the skin barrier, activating cells and promoting tissue regeneration.

The invention discloses a screening method of skin fibroblast marker protein targeting components. The screening method comprises the following steps: (1) constructing a human skin fibroblast cell membrane positioning marker protein data list; (2) obtaining a membrane localization marker protein targeting component; (3) predicting chemical similarity; and (4) molecular docking verification. Database mining, chemoinformatics and molecular docking technologies are comprehensively applied, the HSF membrane localization marker protein beneficial to targeted research is accurately locked, a targeted component set of the HSF membrane localization marker protein is obtained, and an accurate and effective scientific basis is provided for research and development of anti-aging cosmetics.

The application belongs to the field of bioactive peptides, and particularly relates to an egg white peptide sequence QVPLW having a skin damage alleviating effect and application thereof. Specifically, the egg white peptide is prepared by alkaline protease enzymolysis, and after in vitro simulation of gastrointestinal digestion, the sequence thereof is determined by mass spectrometry. The egg white peptide QVPLW can significantly alleviate mechanical damage of skin fibroblasts (HSF) caused by cell scratching. Meanwhile, the egg white peptide QVPLW can also promote the proliferation of HSF cells and the migration of HSF cells in a Transwell chamber. The application provides a new idea for deep processing of egg products and targeted preparation of high-activity egg white peptides, and provides a new perspective for the development of functional foods applied in the auxiliary treatment of skin damage.

The invention relates to the technical field of protein engineering, particularly discloses a human-like fibronectin and a skin filling composition thereof, and provides a recombinant human-like fibronectin, the amino acid sequence of which is as shown in SEQ ID No.1, and the recombinant human-like fibronectin can promote adhesion or migration of skin fibroblasts, so that the human-like fibronectin can be used as a skin filling composition. And the lasting stability of the molecular structure can be maintained in subcutaneous tissues. The skin filling composition containing the recombinant human-like fibronectin can provide supporting and filling functions through subcutaneous injection, so that the purpose of correcting wrinkles is achieved.

The invention provides activity evaluation and application of recombinant humanized III-type collagen, which comprises the following steps of: carrying out cell proliferation, cell adhesion and cell migration experiments on the recombinant humanized III-type collagen by adopting a plurality of cell models, namely human skin fibroblasts HSF, cuticle cells HaCat and mouse fibroblasts 3T3; the bioactivity of the recombinant humanized III-type collagen is subjected to comprehensive and deep analysis from multiple dimensions, so that multi-cell and multi-aspect accurate evaluation is realized, and the biological characteristics and functional expressions of the collagen in different cell environments are accurately captured. Experimental data can provide a powerful experimental basis for the application of the recombinant humanized III-type collagen in the fields of cosmetics, medical instruments and the like, so that the potentials of the recombinant humanized III-type collagen in the aspects of cosmetic effects of improving skin appearance, promoting skin repair and the like are clear; and a key reference can be provided for the properties such as tissue repair and cell adhesion involved in the development of medical apparatuses and instruments.

The invention relates to a CRISPR (clustered regularly interspaced short palindromic repeats)-mediated immortalized sheep fetal skin fibroblast line and application thereof in the field of variation or genetic engineering. The construction of the CRISPR-mediated immortalized sheep fetal skin fibroblast line comprises the following steps: step 1, constructing a Cas9 site-directed knockout plasmid containing an FGF5 gene target sequence; step 2, constructing a homologous repair plasmid Donor-2 kb HA-SV40LT containing a homologous arm of the FGF5 gene; step 3, constructing a donor DNA (Deoxyribose Nucleic Acid) containing an SV40LT protein expression cassette; and step 4, preparing the sheep fetal fibroblast line capable of stably expressing the SV40LT protein at a fixed point. The immortalized SFF-FGF5 / SV40LT monoclonal cell strain created by the invention can become an ideal tool cell line for genome editing research such as sheep CRISPR library screening, multiple gene editing, mutant gene function exploration and the like.

The application of bazedoxifene as an active ingredient in the preparation of a drug for blocking the differentiation of fibroblasts of the IL-6 family / gp130 signal axis. It has been verified that in the microenvironment of skin tissue, the early or very early appearance of pro-fibrotic stimulating factors (such as IL-11 and / or IL-6) can significantly inhibit the initiation of the pro-fibrotic molecular program, avoid the abnormal activation of myofibroblasts, thereby inhibiting the differentiation of fibroblasts into myofibroblasts, and can effectively inhibit the fibroblast migration of skin fibroblasts induced by pro-fibrotic stimulating factors (especially IL-11), effectively prevent skin fibrosis, and thereby prevent the formation of keloid.

The invention discloses application of asiaticoside in preparation of a composition for promoting skin fibroblast repair, and belongs to the technical field of biological medicine. An in-vitro cell scratch repair test quantitatively proves that the asiaticoside or the extract of the asiaticoside can remarkably promote migration and repair of normal human skin fibroblasts in a concentration range of 0.075-0.30 mg / mL for the first time, and the cell viability is good under the concentration. Based on the discovery, the invention further provides a skin repairing composition containing asiaticoside, and the skin repairing composition can be used in skin care products or external preparations to achieve the effects of resisting aging, healing wounds, repairing skin barriers and the like. The invention provides new and firm data support and direction for clinical application and product development of asiaticoside.