10 results about "Skin fibroblast" patented technology

The application provides a culture medium for producing dendrobium stem cell exovesicles and an application thereof, relates to the technical field of biology, and the culture medium for producing dendrobium stem cell exovesicles comprises 0.1-0.5 mg / L naphthalene acetic acid, 0.05-0.2 mg / L thidiazuron, 0.5-5 muM sodium selenate and 1-10 mg / L glutathione. The culture medium for producing dendrobium stem cell exovesicles and the method for preparing dendrobium stem cell exovesicles by using the culture medium for producing dendrobium stem cell exovesicles provided by the application have made significant progress in the yield and quality of dendrobium stem cell exovesicles. Meanwhile, the prepared dendrobium stem cell exovesicles have significant DPPH free radical scavenging capacity and improve the healing capacity of skin fibroblasts.

This invention provides a polypeptide and its application in the preparation of products that promote skin wound repair. The polypeptide of this invention, with the amino acid sequence shown in SEQ ID NO:1, not only possesses excellent anti-inflammatory and antioxidant activities, making it suitable for the preparation of anti-inflammatory and antioxidant products, but also effectively promotes the migration of skin fibroblasts and accelerates wound healing, making it suitable for the preparation of products that promote skin wound repair.

The application of bazedoxifene as an active ingredient in the preparation of a drug for blocking the differentiation of fibroblasts of the IL-6 family / gp130 signal axis. It has been verified that in the microenvironment of skin tissue, the early or very early appearance of pro-fibrotic stimulating factors (such as IL-11 and / or IL-6) can significantly inhibit the initiation of the pro-fibrotic molecular program, avoid the abnormal activation of myofibroblasts, thereby inhibiting the differentiation of fibroblasts into myofibroblasts, and can effectively inhibit the fibroblast migration of skin fibroblasts induced by pro-fibrotic stimulating factors (especially IL-11), effectively prevent skin fibrosis, and thereby prevent the formation of keloid.

The application relates to the field of cell freezing, and discloses a skin fibroblast freezing device. In the application, the inner surface of a main body shell is fixedly connected with a freezing cabin, the outer surface of the freezing cabin is fixedly connected with an air outlet cabin, the outer surface of the air outlet cabin is fixedly connected with a one-way air valve, the outer surface of the one-way air valve is fixedly connected with an air guide pipe, the outer surface of the air guide pipe away from the one-way air valve is fixedly connected with an air inlet fan, and the inner surface of the main body shell at the input end of the air inlet fan is detachably connected with an activated carbon sieve plate. When the one-way air valve is opened, the rotation of the air inlet fan drives external air to enter, impurities and water molecules are filtered at the activated carbon sieve plate, the air is flushed into the air outlet cabin through the air guide pipe, dry air is extruded from the air outlet cabin to the sealing cabin door, the sealing cabin door is covered when the placing box is pushed in, hot air is extruded together with water vapor, and air flows out of the cabin to open the space for receiving the freezing cabin.

This invention discloses a recombinant protein pPIC9K-17, its preparation method, and its applications, relating to the field of gene recombination technology. The recombinant protein pPIC9K-17 provided by this invention does not strictly follow the (Gly-X-Y)n triple helix repeat pattern of traditional collagen in its core structural unit. The repeating unit sequence includes a "GAIQ" sequence and a sequence containing collagen characteristics, possessing a certain rigid structural basis and containing receptor-binding or signal activation domains for specific growth factors. The direct tandem connection of multiple such units theoretically enables multivalent binding, enhancing affinity and signal activation efficiency with cell surface receptors, thus surpassing the biological functional limitations of single components (pure collagen or pure growth factors) or simple repeating structures. The recombinant protein of this invention not only effectively promotes the proliferation and migration of skin fibroblasts and accelerates collagen synthesis, but also shows clear efficacy in promoting hair regeneration (hair growth), and can be used to prepare products for skin repair and / or promoting hair regeneration.

The present invention relates to a liposome-based mRNA delivery system having an effect of improving skin elasticity and improving wrinkles, and use thereof. The liposome-based mRNA delivery system according to the present invention provides an excellent effect in improving skin elasticity and wrinkles, and can maximize the efficacy of preventing skin aging and regeneration. In addition, the system of the present invention stably encapsulates and effectively delivers mRNA to skin cells, providing higher efficiency and safety than existing methods. In other words, in the present invention, synthetic mRNA is wrapped in a liposome-based nanoparticle and stably delivered into skin cells, thereby inducing collagen synthesis in skin fibroblasts and improving skin structure.

The application belongs to the technical field of cell culture, and particularly relates to a kind of extracellular vesicles containing active mitochondria and a preparation method thereof.The preparation method specifically comprises the following steps: culturing mitochondrial donor cells until the fusion degree reaches 70-80%, discarding the original culture medium, adding DMEM / F12 culture medium containing exosome-free fetal bovine serum, verbascoside and lupeol for continuous culture; collecting cell culture supernatant, and after multiple centrifugation and microporous filter filtration, centrifuging to collect the precipitate, thereby obtaining extracellular vesicles rich in active mitochondria.The extracellular vesicles obtained by the preparation method of the application can significantly promote the proliferation of UVB-induced photoaging human skin fibroblasts, inhibit the expression of senescence-associated proteins p16 and p21, have the effects of repairing photoaging damage and reversing cell senescence, and have a broad application prospect.

The application discloses a spermidine composition with an anti-aging effect and a preparation method thereof, and relates to the technical field of medical cosmetics. The spermidine composition with the anti-aging effect is composed of the following components in mass parts: 5-15 parts of spermidine, 3-8 parts of fucoxanthin, 1-4 parts of a SASP inhibitor, 10-20 parts of nicotinamide, 2-6 parts of sodium hyaluronate, 12-25 parts of soybean lecithin and 3-5 parts of ergothioneine. The SASP inhibitor is a compound with a specific structure. The composition can synergistically act on multiple pathways such as cell autophagy, oxidative stress and aging-related secretory phenotype by compounding spermidine, fucoxanthin and the specific SASP inhibitor, and compared with a compounding scheme of single components or other alternative components, the composition shows a more optimal comprehensive effect in protecting skin fibroblasts and delaying cell aging.

This invention discloses a synergistic extraction process for highly active white jade snail enzymes, belonging to the field of bioenzyme extraction technology. Using the soft tissue and foot gland mucus of high-quality white jade snails as composite raw materials, an integrated technical route of "low-temperature and mild pretreatment – ​​ultrasonic-microwave synergistic cell disruption – directional hydrolysis of composite enzymes – gradient membrane separation and purification – vacuum freeze-drying" is employed. The core process parameters are: synergistic action of 20kHz ultrasound and 2450MHz microwaves at a power ratio of 2:1, combined with a composite enzyme system composed of papain and bromelain at a mass ratio of 1:0.8, achieving a comprehensive enzyme extraction rate of over 94.2%; deep pre-freezing at -60℃ and vacuum sublimation drying, resulting in an enzyme activity retention rate ≥96.5%. The finished product is a off-white powder with water solubility ≥99.3%, protease specific activity ≥1800U / mg, skin fibroblast proliferation promotion rate ≥45%, and no organic solvent residue, making it suitable for high-end cosmetics, biocatalysis, and functional health products.

This invention discloses an ergothioneine composition for promoting collagen production and its preparation method, relating to the fields of biotechnology and skincare technology. The composition comprises the following components in the indicated mass ratios: 0.1-1 parts ergothioneine, 0.01-0.1 parts a specific PPARδ agonist, 1-5 parts collagen peptides, 0.5-3 parts ascorbic acid or its derivatives, 0.1-0.5 parts chondroitin sulfate, and 90-95 parts a buffered aqueous solution; the collagen peptides comprise type I and type III collagen peptides in a mass ratio of 10:1 to 2:1. Through the synergistic effect of multiple components such as ergothioneine, the specific PPARδ agonist, and collagen peptides at the molecular and system levels, this composition constructs a stable and sustained-release delivery system, effectively promoting the synthesis of type I and type III collagen by skin fibroblasts.