150 results about "Skin fibroblast" patented technology

The invention discloses aloe exosome-like nano-vesicles capable of delaying skin photoaging and an extraction method and application thereof, and belongs to the technical field of exosome-like nano-vesicle extraction. The extraction method of the aloe exosome-like nano-vesicles is designed and optimized, the yield of the aloe exosome-like nano-vesicles is obviously improved, and the extracted aloe exosome-like nano-vesicles can obviously delay skin photoaging and have an obvious improvement effect on skin fibroblast aging damage caused by ultraviolet radiation; a potential treatment means is provided for preventing or treating actinic keratosis caused by ultraviolet radiation and further possible squamous cell carcinoma.

The invention relates to the technical field of biological medicine, in particular to recombinant III-type humanized collagen as well as a preparation method and application thereof. In one embodiment, the recombinant III type humanized collagen prepared by the invention has an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID NO: 4. The recombinant III-type humanized collagen prepared by the invention has good biological activity, can promote collagen synthesis related gene expression, promote skin fibroblast proliferation, increase the content of skin collagenous fibers and elastic fibers, and improve skin elasticity and compactness; in addition, the expression of osteogenesis related genes is promoted, and the proliferation of osteoblasts is promoted. The results show that the recombinant III-type humanized collagen prepared by the invention has an extremely wide application prospect in developing related medical and cosmetic products for delaying skin aging and promoting wound repair and bone tissue healing.

A biosynthesis method for preparing royal jelly acid and the use thereof in skin care, which belong to the technical field of genetic engineering and biosynthesis. The royal jelly acid is produced by means of fermentation on the basis of yeast engineering bacteria using a culture medium containing trans-2-decenoic acid. An assessment at both skin cell level and skin model level shows that royal jelly acid can promote the synthesis of stratum corneum lipids and ceramide on aging skin and repair the skin barrier; and also can promote the synthesis of mitochondria ATP and NADPH in skin fibroblasts, reduce the active oxygen content of mitochondria, improve mitochondrial dysfunctions, and inhibit apoptosis. Further experiments show that royal jelly acid can repair DNA damage of fibroblasts caused by ultraviolet irradiation, reduce telomere shortening caused by cell division and replication, and strengthen the protection of chromosomal terminal regions. The prepared royal jelly acid has wide application prospects in the development of functional skin care products for skin repairing, aging resisting, etc.

The invention discloses an anti-aging composition derived from space breeding desert algae as well as a preparation method and application of the anti-aging composition, and relates to the technical field of application of desert algae. The anti-aging composition is an extract of Hassalliasp.P06 after space breeding, and the preservation number of the Hassalliasp.P06 is CCTCC (China Center for Type Culture Collection) M20242054, and the preservation number of the Hassalliasp.P06 is CCTCC M20242054. The anti-aging composition derived from the space breeding desert algae comprises the following components: polypeptide, free amino acid, polysaccharide and cyclic adenosine monophosphate. The skin care product has very strong moisturizing capacity, can promote the proliferation of skin fibroblasts and remarkably improve the skin compactness, has a soothing effect and oxidation resistance, and can be applied to skin care products or cosmetics to be used as a compactness agent or an anti-aging agent so as to improve the skin aging symptom.

The invention discloses a composition for delaying cell senescence, and the composition for delaying cell senescence comprises fisetin, urolithin A and ergothioneine, or consists of fisetin, urolithin A and ergothioneine. The invention also discloses an application of the composition for delaying cell aging in preparation of a product for delaying skin fibroblast aging, and an anti-aging preparation containing the composition. According to the composition for delaying cell aging, fisetin, urolithin A and ergothioneine contained in the composition can generate a synergistic effect, aging of skin fibroblasts can be remarkably delayed, and healing and repairing of skin are promoted.

The invention belongs to the technical field of cell small molecule compound reprogramming and bone tissue engineering material culture, and discloses a method for inducing fibroblasts to be reprogrammed into osteoblasts. According to the invention, small molecule compounds act on fibroblasts, each small molecule is endowed with characteristics of a set of characteristic gene expression mode, and the small molecules are divided into different modules according to the expression characteristic mode, so that the fibroblasts of the cells are converted into osteogenic differentiation, the osteogenic differentiation effect is enhanced and maintained, and the in-vivo development of the cells is simulated to a great extent; therefore, healthy osteoblasts can be prepared from the skin fibroblasts, cell stress pressure can be resisted, and the prepared cells are free of tumorigenicity, safe and healthy. The osteoblast disclosed by the invention can be applied to preparation of bone materials and medicines for treating bone defects, for example, the osteoblast and a traditional bone material are used for constructing an active bone tissue engineering material, so that the use of allogeneic bones commonly used in clinic at present is avoided, and the occurrence of adverse ethical events is reduced.

Provided is a collagen production promoter comprising, as an active ingredient, a triglyceride represented by the following formula (I), wherein R1, R2 and R3 each denotes a saturated fatty acid residue, at least one of which is a pentadecanoic acid residue. The triglyceride comprising the pentadecanoic acid promotes the production of skin fibroblasts and collagen, and can be used as a topical skin care composition and a beauty food or beverage for improving the condition of skin.

The invention provides application of recombinant collagen in preparation of a preparation for increasing the expression quantity of skin fibroblast proliferation related genes. The amino acid sequence of the recombinant collagen is shown as SEQ ID NO: 1. The expression condition of a series of genes capable of enhancing skin fibroblast proliferation is detected in a transcriptomics mode, and it is proved that the recombinant collagen can promote expression of the skin fibroblast proliferation genes. The collagen can be used for development and application of various preparation products, and endows the preparation products with the efficacy and effect of promoting skin fibroblast proliferation.

The invention discloses an application of bulbus lilii glycoside B. The application is that bulbus lilii glycoside B is used as a unique active component or one of the active components to prepare a product for preventing or / and treating skin photoaging diseases. Experimental results of the invention show that lilium brownii glycoside B can significantly improve the cell viability of UVA-induced human skin fibroblasts, can reduce the number of beta-galactosidase positive cells in the UVA-induced human skin fibroblasts and reduce lipofuscin deposition, and can significantly improve the photoaging of the UVA-induced skin fibroblasts. Meanwhile, the skin photoaging grading of the back of a C57BL / 6 mouse induced by UVA can be remarkably improved. Therefore, the bulbus lilii glycoside B is expected to be used as an active ingredient for preparing medicines or cosmetics for preventing or / and treating the skin photoaging diseases, and particularly has important significance and remarkable application value for preventing or / and treating the UVA-induced skin photoaging diseases.

The invention provides a retinyl oligopeptide derivative with anti-aging and wrinkle fading functions and application thereof, and belongs to the technical field of polypeptide derivative application, the retinyl oligopeptide derivative is obtained by connecting a carboxyl terminal of retinoic acid and an amino terminal of oligopeptide, and has a structure as shown in a formula I. In the formula I, n is 1 or 2; l is oligopeptide. The retinoic acid modified oligopeptide derivative provided by the invention has good solubility in both a water phase and an oil phase, is beneficial to product formula design in an application process, has good antioxidant activity and skin cell injury protection activity, can reduce MMP2 and TNF alpha expression increased by ROS in skin fibroblasts, and has a good application prospect. The composition has excellent activity of promoting collagen expression, and has good effects of resisting oxidation, repairing skin injury and smoothing wrinkles when being used for cosmetics or skin care products.

The invention discloses lactobacillus pentosus capable of converting various substances of dendrobium officinale to generate rich active small molecules and improving skin health, and belongs to the technical field of microorganisms. The lactobacillus pentosus (Lactobacillus pentosus) CCFM1482 obtained by screening can be used for producing various active substances by fermenting dendrobium officinale and regulating and controlling the synthesis of skin collagen factors, such as HSF cells (human skin fibroblasts), MDA (malondialdehyde), Nrf2 (nuclear factor E2 related factor 2) and HaCaT cells (human keratinocytes). Therefore, the lactobacillus pentosus CCFM1482 fermented dendrobium officinale kimura ex migo fermentation liquor has a huge application prospect in the preparation of products for preventing or treating the skin quality, and the dendrobium officinale kimura ex migo fermentation liquor can be used for preparing the products for preventing or treating the skin quality.

The invention discloses a preparation method of a three-dimensional umbilical cord mesenchymal stem cell exosome and application of the three-dimensional umbilical cord mesenchymal stem cell exosome in the fields of medicines and medical beauty. The preparation method comprises the step of amplifying umbilical cord mesenchymal stem cells (UC-MSCs) by adopting a three-dimensional culture system. Experiments show that IGF-1, EGF, VEGF, TGF-beta, PDGF, FGF and the like in the exosome subjected to three-dimensional culture are beneficial to skin repair and oxidation resistance, and the content of cell active factors for promoting collagen secretion is remarkably increased. The umbilical cord mesenchymal stem cell exosome significantly improves the fibroblast proliferation activity, and UV-induced skin fibroblast damage model construction and function verification experiments also prove that the exosome can promote the HA level recovery of damaged skin fibroblasts. The invention provides an efficient and safe biological material for tissue repair.

The present invention relates to a composition comprising plant extracts. The composition according to the present invention has a skin whitening effect by reducing the total amount of melanin and tyrosinase activity in melanocytes of the skin, promotes skin regeneration and increases skin elasticity or reduces skin wrinkles by promoting collagen synthesis and inhibiting collagenase activity in fibroblasts of the skin, has an anti-inflammatory effect or a skin soothing effect by inhibiting NO generation, increases the amount of moisture in the skin and has a moisturizing effect by promoting generation of hyaluronic acid in fibroblasts, and has an antioxidant effect by scavenging free radicals. In addition, since the composition of the present invention exhibits a broad antibacterial effect against various bacteria, the composition may be used as a cosmetic composition, a pharmaceutical composition, a skin external preparation, or a food composition.

The invention discloses an active peptide with an oxidative stress regulation effect as well as preparation and application of the active peptide. The amino acid sequence of the active peptide is Ala-Val-Pro-Ile-Cys-Ala-Val-Pro. The preparation method of the active peptide comprises the following steps: collecting pearl shells, obtaining an extract, collecting supernate, selecting, screening and synthesizing polypeptide. The active peptide with the oxidative stress regulation function has high activity, free radicals can be well removed, and damage of H2O2 to skin fibroblasts is relieved; the skin wound healing can be promoted. The active peptide with the oxidative stress regulation effect has a relatively good application prospect in drugs or instruments for relieving cell oxidative stress.

The invention discloses a pharmaceutical composition with skin repairing, anti-inflammatory and anti-aging effects as well as a preparation method and application of the pharmaceutical composition. The pharmaceutical composition mainly comprises a stem cell exosome and a fusion polypeptide-single domain antibody conjugate, and can also comprise proline and pharmaceutically acceptable auxiliary materials. The amino acid sequence of the fusion polypeptide is as shown in SEQ ID NO.1, and the fusion polypeptide can specifically target skin cells; the single-domain antibody is an anti-IL-6 single-domain antibody, the amino acid sequence of the single-domain antibody is shown as SEQ ID NO.2, and the single-domain antibody can efficiently inhibit inflammatory factors. Experiments show that through the synergistic effect of all the components, the composition remarkably promotes proliferation of skin fibroblasts and keratinocytes, inhibits secretion of inflammatory factors such as IL-6 and TNF-alpha, promotes synthesis of collagen, effectively improves skin aging and inflammation states and has wide application prospects in the fields of skin repair, inflammation resistance and senescence resistance.

The invention discloses an exosome composition as well as a method and application thereof for promoting collagen synthesis, and particularly relates to the technical field of cosmetics, the exosome composition comprises the following components in parts by weight: 15-30 parts of human umbilical cord mesenchymal stem cell exosome, 8-15 parts of radix scutellariae extract, 2-6 parts of acetyl hexapeptide-2 and 1-4 parts of zinc gluconate, the composition can also comprise a humectant and a thickening agent. The preparation method comprises the following steps: preparing the human umbilical cord mesenchymal stem cell exosome, dissolving the radix scutellariae extract, the acetyl hexapeptide-8 and the zinc gluconate, mixing with the exosome, adding the humectant and the thickening agent, adjusting the pH value, and removing bacteria. Through the synergistic effect of all the components, the composition can effectively promote skin fibroblasts to synthesize type I collagen, has good anti-aging and anti-inflammatory effects, is high in safety and can be widely applied to preparation of anti-aging skin care products.

The invention belongs to the technical field of cosmetics, and provides a composition with an anti-wrinkle effect and application of the composition in preparation of cosmetics. The composition provided by the invention comprises a mulberry root extract and a recombinant human collagen III, and the mass part ratio of the mulberry root extract to the recombinant human collagen III is (2-20): 1. In the composition provided by the invention, the mulberry root extract and the recombinant human collagen III have a synergistic anti-wrinkle effect, and the effect of the composition is obviously greater than the sum of the effects of singly using the mulberry root extract and singly using the recombinant human collagen III in the aspect of promoting the synthesis of the skin fibroblast collagen I. Therefore, when the composition with the anti-wrinkle effect provided by the invention is used for preparing cosmetics, the obvious anti-wrinkle effect can be obtained under the conditions of reducing the use amount of the recombinant human collagen III and saving the cost.

The invention belongs to the technical field of biological medicines, and particularly relates to application of MIM2 and derivatives thereof in preparation of products for treating tissue fibrosis. It is found that the compound MIM2 can effectively inhibit expression and synthesis of various cancers (extracellular matrix proteins such as collagen and laminin in cells). In-vitro experiments show that after MIM2 treatment, collagen and laminin expression of the cancer cells, skin fibroblasts, hepatic stellate cells and embryonic lung cells is remarkably reduced. Animal experiments further prove that the MIM2 can effectively inhibit collagen deposition in tumor tissues of tumor-bearing mice, can slow down the fibrosis process of liver fibrosis and pulmonary fibrosis mouse models, and can reduce the level of plasma hyaluronic acid at the same time. The results show that the MIM2 has the effects of inhibiting extracellular matrix deposition and delaying tissue fibrosis, and is expected to be developed into small molecular drugs for treating cancer-related fibrosis and other fibrosis diseases.

The invention relates to the technical field of biomedicine, in particular to saccharified modified I-type collagen and a preparation method and application thereof.The preparation method of the saccharified modified I-type collagen comprises the following steps that 1, freeze-dried I-type collagen is dissolved in a PBS acid solution, then the pH is adjusted to 6.5-7.5 with an alkali solution, and an I-type collagen solution is obtained; and (2) adding methylglyoxal into the I-type collagen solution, and incubating for 25-35 days in a dry normal-temperature environment in a dark place to obtain the saccharified modified I-type collagen. The saccharified modified I-type collagen prepared by the invention can effectively simulate the pathological state of diabetic skin saccharified collagen accumulation so as to promote skin fibroblasts to generate pathological changes. The saccharified modified I-type collagen can serve as an extracellular matrix, and compared with glucose modeling, the saccharified modified I-type collagen can better simulate the pathological environment of diabetic skin saccharified collagen accumulation in vitro.

A composition for inhibiting functions of various types of matrix metalloproteinases in skin and a use thereof. The composition comprises one or more of the following components: epigallocatechin gallate (EGCG), dihydroquercetin, sugarcane polyphenol, coix seed powder, ginseng, or galangal. The composition has the effects of inhibiting expression of various types of matrix metalloproteinases in skin fibroblasts, inhibiting activity of matrix metalloproteinases, improving collagen content, and inhibiting elastase, and does not have obvious side effects. The composition can be used for preparing various cosmetics, functional foods and health care products related to skin aging resistance, and has a wide application prospect.

The invention discloses an application of a POGLUT1 gene in reversing senescence of skin fibroblasts. According to the application, by down-regulating expression of POGLUT1 in skin fibroblasts, SA-beta-Gal activity and expression of senescence marker genes p16, p21 and p53 can be reduced, the cell apoptosis number is reduced, and the anti-inflammatory capacity of HSFs is improved, so that the effect of reversing senescence of the skin fibroblasts is achieved. The invention discloses POGLUT1 as a core regulatory gene of MSCs for reversing skin aging, and a molecular regulatory network of the POGLUT1 in a skin aging process through a Notch signal channel, and clarifies a potential molecular mechanism of MSCs for reversing skin aging. The invention provides a new molecular target for clinically treating skin aging related diseases, also provides a new theoretical basis for understanding the effect of MSCs in skin aging, and also lays an important experimental foundation for clinically developing an anti-aging treatment strategy based on MSCs or POGLUT1 targets.

This application provides a barrier-repairing composition and its use. The composition comprises panthenol, niacinamide, folic acid, and cobalamin in a mass ratio of 3-7:1-5:0.0005-0.1:0.00005-0.01. This application also provides uses of the composition and products containing the same. The composition utilizes panthenol, niacinamide, folic acid, and cobalamin in combination, where the components complement and enhance each other, achieving a synergistic effect in upregulating BMAL2 gene expression. This enhances the DNA damage repair capacity of skin fibroblasts and potentially promotes barrier repair. Furthermore, the composition provided herein can significantly reduce the expression of capsaicin receptor 1 (TRPV1) and increase the expression of involucrin, PIEZO1, and TNXB, reducing skin discomfort and enhancing skin barrier function. Furthermore, the composition provided herein can reduce the expression of inflammatory factors, demonstrating its anti-inflammatory effect.

The present application relates to a CRISPR-mediated immortalized sheep fetal skin fibroblast cell line in the field of variation or genetic engineering and application thereof.The construction of the CRISPR-mediated immortalized sheep fetal skin fibroblast cell line of the present application comprises the following steps: step one, constructing a Cas9 site-directed knockout plasmid containing an FGF5 gene target sequence; step two, constructing a homologous repair plasmid Donor-2 kb HA-SV40LT containing an FGF5 gene homologous arm; step three, constructing a donor DNA containing an SV40LT protein expression frame; and step four, preparing a sheep fetal fibroblast cell line stably expressing SV40LT protein.The immortalized SFF-FGF5 / SV40LT monoclonal cell strain created by the present application can become an ideal tool cell line for genome editing researches such as sheep CRISPR library screening, multiple gene editing and mutation gene function exploration.

The application relates to a recombinant type I collagen and application thereof, which utilizes the characteristics of Pichia pastoris capable of secreting and expressing recombinant proteins, secretes the recombinant type I collagen on the culture medium supernatant, and reduces the difficulty of protein purification. In addition, the integrin site RGD is inserted into the collagen to form a recombinant type I collagen with high stability and high expression. According to the relative proliferation of cells and the relative adhesion experiment of cells, it can be seen that the recombinant type I collagen has the effects of promoting the proliferation of skin fibroblasts, improving the skin, promoting wound healing, promoting the adhesion of fibroblasts in the skin layer and the like. Therefore, the recombinant type I collagen has great potential applications in the fields of skin care and beauty, medical treatment and the like.

The invention discloses an application of a sesquiterpene lactone compound in preparation of a medicine for enhancing a radiation therapy effect, and belongs to the technical field of biomedicine.The elephantopus tomentosus monomer EM-2 in a formula (I) enhances G2 / M phase retardation caused by ionizing radiation by inhibiting a signal channel caused by the ionizing radiation, so that the sensitivity of the radiation therapy is remarkably improved. Experimental results show that the compound combined with radiotherapy can reduce the expression of a cell proliferation marker Ki67, reverse ionizing radiation induced p-AKT activation, and further enhance the anti-tumor effect of radiotherapy. The compound has a good proliferation inhibition effect on cervical cancer cells, and has low toxicity on normal human skin fibroblast HSF cells. On the other hand, EM-2 can be combined with AKT protein in a targeted mode to inhibit a PI3K / AKT signal transduction pathway activated by radiotherapy, meanwhile, protective autophagy activated after radiotherapy is inhibited, and the sensitivity of ionizing radiation to cervical cancer cells is improved.

The present invention provides a method and application of inducing differentiation of iPSC into vascular endothelial cells. Furthermore, the monoclonal antibody specifically targeting ALK5 provided by the present invention can effectively improve the efficiency of inducing skin fibroblasts to generate iPSCs; and the induced iPSCs have good ability to differentiate into vascular endothelial cells, and have good application prospects.

The utility model discloses a skin fibroblast culture bottle which comprises a bottle body, a bottle neck is integrally formed at one end of the bottle body, a ventilation structure is in threaded connection with the outer side of one end of the bottle neck, a clamping seat is integrally formed at the top end of the bottle body, a clamping ring is integrally formed at the bottom end of the bottle body, and a culture structure is arranged on the outer side of the bottle body. A marking structure is installed on one side of the bottle body. According to the cell culture bottle, the rotatable ventilation valve is mounted at one end of the bottle cap, and the bottle cap and the ventilation valve are provided with the ventilation holes, so that air inside and outside the cell culture bottle can be exchanged when the ventilation holes in the bottle cap and the ventilation valve are overlapped, and air exchange can be stopped when the ventilation holes in the bottle cap and the ventilation valve are staggered by rotating the ventilation valve; and the polytetrafluoroethylene filter membrane in the bottle cap can ensure the entry of carbon dioxide and the discharge of oxygen and prevent microorganisms and pollutants from entering, so that the aim that the skin fibroblast culture bottle can ensure the air permeability of a growth environment is fulfilled.

The application provides a culture medium for producing dendrobium stem cell exovesicles and an application thereof, relates to the technical field of biology, and the culture medium for producing dendrobium stem cell exovesicles comprises 0.1-0.5 mg / L naphthalene acetic acid, 0.05-0.2 mg / L thidiazuron, 0.5-5 muM sodium selenate and 1-10 mg / L glutathione. The culture medium for producing dendrobium stem cell exovesicles and the method for preparing dendrobium stem cell exovesicles by using the culture medium for producing dendrobium stem cell exovesicles provided by the application have made significant progress in the yield and quality of dendrobium stem cell exovesicles. Meanwhile, the prepared dendrobium stem cell exovesicles have significant DPPH free radical scavenging capacity and improve the healing capacity of skin fibroblasts.

This invention provides a polypeptide and its application in the preparation of products that promote skin wound repair. The polypeptide of this invention, with the amino acid sequence shown in SEQ ID NO:1, not only possesses excellent anti-inflammatory and antioxidant activities, making it suitable for the preparation of anti-inflammatory and antioxidant products, but also effectively promotes the migration of skin fibroblasts and accelerates wound healing, making it suitable for the preparation of products that promote skin wound repair.

The application belongs to the technical field of antibodies, and particularly relates to an antibody for specifically treating orf of milk goat kids and application thereof. Virus liquid of a diseased goat is collected from a lesion site, and ORFV virus strains are obtained through subculture and amplification of goat skin fibroblasts; milk goats are immunized with a weak vaccine to prepare high-titer immune serum; flow sorting technology is used to separate ORFV-specific B cells from PBMC (peripheral blood mononuclear cells); antibody genes are amplified through nested PCR, expression vectors are constructed, and 293T cells are transfected to express monoclonal antibodies. The monoclonal antibody No. 2 obtained in the application has high affinity and specificity, the heavy chain CDR1-3 region sequence is SEQ ID NO: 1-3, and the light chain CDR1-3 region sequence is SEQ ID NO: 4-6. The antibody can be used for diagnosis, treatment and prevention of orf of goats, and has the characteristics of high efficiency, specificity and stability, and provides a new biological preparation for prevention and control of orf of goats.