237 results about "Exovesicles" patented technology

Disclosed are methods of treating traumatic brain injury in a subject by administering a therapeutic MSC secretome product made by a method comprising culturing bone marrow-derived MSCs under conditions that include oxygen tension below 5% and a culture media with a pH below 7.

Disclosed are methods of treating infertility or increasing fertility of a female subject by administering a therapeutic MSC secretome product made by a method comprising culturing bone marrow-derived MSCs under conditions that include oxygen tension below 5% and a culture media with a pH below 7.

The invention provides a method for separating extracellular vesicles from a milk source, which comprises the step of adding a dispersing agent before adding a precipitator into the milk source to precipitate casein so as to reduce the wrapping of protein precipitation on the extracellular vesicles and improve the yield of the extracellular vesicles, thereby obtaining a milk source extract rich in the extracellular vesicles.

The invention belongs to the technical field of stem cell culture and exosome extraction, and provides an RELM beta pre-activated stem cell outer vesicle with high anti-inflammatory and anti-fibrosis activity. According to the method, the mesenchymal stem cells are subjected to hunger culture after being adhered to the wall and then are continuously cultured by a complete culture medium containing RELM beta factors, then supernate is collected, and the exovesicles are obtained through separation. In the preparation process of the extracellular vesicles, the paracrine function of MSC cells is promoted by the RELM beta, the secretion of anti-inflammatory and anti-fibrosis active factors is obviously activated, and the generation of the extracellular vesicles with enhanced anti-inflammatory and anti-fibrosis effects is promoted. Gene modification is not involved, toxic biochemical reagents or materials are not added, exogenous operation steps are simple and convenient, clinical application is guaranteed through sterile operation, no RELM beta residues exist after extracellular vesicle extraction, and the method is safe, non-toxic and effective.

The invention provides a rapid quantitative analysis method for an outer vesicle proteome, and belongs to the technical field of quantitative analysis of the outer vesicle proteome. According to the rapid quantitative analysis method for the outer vesicle proteome, firstly, a high-quality outer vesicle sample is obtained through low-speed centrifugation, ultra-speed centrifugation and membrane filtration; and obtaining proteome data by utilizing two-dimensional electrophoresis and mass spectrometry. Then, a mathematical model equation set is constructed for preliminary analysis, a protein interaction network is optimized through an embedded game optimization index model, and feature extraction and quantitative analysis are conducted through a convolution parameter adaptive function and a residual network deep learning model; finally, a quantitative analysis result containing the protein expression level and the function importance is generated in combination with the multi-dimensional protein expression contribution index, and the technical problem that in the prior art, in the rapid quantitative analysis process of the outer vesicle proteome, the protein component complexity is high, and consequently the quantitative precision is low is solved.

The invention discloses a preparation method of artemisia apiacea extracellular vesicles, which comprises the following steps: step 1, pre-treating artemisia apiacea, carrying out primary centrifugal treatment, removing large plant tissues and cell debris, carrying out secondary centrifugal treatment, filtering by a needle filter, and collecting; step 2, transferring the filtered suspension to a sucrose density gradient solution, then carrying out third centrifugal treatment, collecting the solution with the concentration of 30% in the layer, and carrying out fourth centrifugal treatment to obtain artemisia apiacea extracellular vesicles; the invention further discloses an artemisia apiacea extracellular vesicle, a pharmaceutical composition for treating cerebral apoplexy, a medicine and application of the artemisia apiacea extracellular vesicle in preparation of the medicine for treating cerebral apoplexy. The naturally-sourced vesicle delivery system provided by the invention is expected to have higher safety and lower toxic and side effects, and the enrichment concentration of the therapeutic component at the focus part of cerebral apoplexy is improved, so that the bioavailability and the treatment efficiency of the medicine are remarkably improved.

The invention relates to the technical field of biological medicine, in particular to a lactobacillus-sourced extracellular vesicle and application of the lactobacillus-sourced extracellular vesicle in prevention and treatment of necrotizing enterocolitis, the extracellular vesicle is prepared from lactobacillus, the lactobacillus is Lactobacillus rhamnosus GG, the strain is purchased in the American Center for Type Culture Collection, and the preservation number is ATCC 53103. The preparation method of the extracellular vesicles comprises the following steps: carrying out ultracentrifugation on a lactobacillus culture solution, removing thalli, then collecting supernate, carrying out primary microfiltration, taking micro-filtrate, carrying out differential centrifugation, resuspending precipitates by using PBS, then carrying out secondary microfiltration, collecting the micro-filtrate, carrying out density gradient centrifugation, and collecting a 30-45% interface vesicle layer. The invention discloses a synergistic effect mode between intestinal symbiotic flora and intestinal epithelial cell metabolic molecules for the first time, and the discovery is expected to construct a brand-new mechanism normal form for regulating the host intestinal barrier function by flora.

Disclosed are methods of treating Ehlers-Danlos Syndrome in a subject by administering a therapeutic MSC secretome product made by a method comprising culturing bone marrow-derived MSCs under conditions that include oxygen tension below 5% and a culture media with a pH below 7.

The invention discloses a kit for detecting miRNAs of multiple extracellular vesicles based on a TtAgo-driven rolling circle amplification technology and application of the kit, a platform combines TtAgo protein and RCA reaction, cooperates with endonuclease activity and DNA polymerase activity of TtAgo, accurately controls reaction kinetic balance between temperature and enzyme activity, not only does not damage the cutting activity of TtAgo, but also can detect miRNAs of multiple extracellular vesicles. The method provided by the invention can be used for detecting multiple extracellular vesicles miRNAs, ensures strong polymerization amplification performance, creates a strong one-step single-tube experiment, greatly simplifies the experimental process, shortens the detection time, realizes ultra-sensitive and specific detection of nucleic acid markers, and can be used for detecting multiple extracellular vesicles miRNAs.

A method for producing extracellular vesicles (EV) in large quantities by using a peptide derived from Noxa protein, which plays a key role in apoptosis, and derivatives thereof, are described. Also described are the use of the peptide for promoting extracellular vesicle production and methods for producing extracellular vesicles by using the same. The methods of the present invention can efficiently and uniformly be used to mass-produce extracellular vesicles and can be used to produce extracellular vesicles which load drugs that pass poorly through cellular membranes, as well as recombinant proteins or plasmid DNA.

The invention relates to an extracellular vesicle composition for aiding sleep as well as a preparation method and application thereof. The composition comprises two or more of passion flower extracellular vesicles, chamomile extracellular vesicles and liquorice extracellular vesicles. According to the extracellular vesicle composition, a combined formula is adopted, and the CL-level of cells is improved, the expression level of GABA related proteins is improved, the expression of brain-derived neurotrophic factors (BDNF) of the cells is promoted, and the synaptic growth of neuronal cells is promoted in a multi-dimensional combined mode that the passiflora edulis is compounded with multiple Chinese herbal medicine extracellular vesicles; the sleep-aiding pillow has the advantages that multi-aspect efficient sleep-aiding effects can be realized, sleep time can be shortened, and sleep quality can be improved. Meanwhile, the extracellular vesicle composition disclosed by the invention adopts nano-scale raw materials, has good permeability, and is more easily absorbed and ingested by cells and skin to play a role.

A method involves recovery an extracellular vesicle from an extracellular vesicle-containing sample. Such a method of recovering the extracellular vesicle may include separating the extracellular vesicle from the extracellular vesicle-containing sample in the presence of a polymer.

The invention provides a preparation method of high-purity extracellular vesicles, and belongs to the technical field of extracellular vesicles, and the preparation method comprises the following steps: collecting cell culture supernatant generated by culturing cells, and setting a process parameter combination based on an orthogonal test principle; centrifuging the cell culture supernate for the first time; carrying out secondary centrifugation on the first centrifugal supernatant; filtering and purifying the first extracellular vesicles to obtain second extracellular vesicles; purifying the second extracellular vesicle to obtain a third extracellular vesicle; concentrating the third extracellular vesicles to obtain fourth extracellular vesicles; performing characteristic detection on the fourth extracellular vesicles, and recording detection data; establishing a preparation process physical and chemical model; performing parameter fitting on the preparation process physical and chemical model to obtain an optimal process parameter combination; carrying out an extracellular vesicle preparation experiment by adopting the optimal process parameter combination, and correcting a preparation process physical and chemical model to obtain a final process parameter combination; and preparing the extracellular vesicles.

The invention relates to a real-time enrichment and detection method for extracellular vesicles. Belongs to the technical field of biological detection. The preparation method comprises the following steps: firstly, preparing a silicon dioxide colloidal crystal (SCC) thin film by adopting a vertical evaporation method; then modifying distearoyl phosphatidyl ethanolamine-polyethylene glycol-carboxyl (DSPE) on the silicon dioxide colloidal crystal film, and separating and enriching the extracellular vesicles in the biological liquid by utilizing the affinity characteristic of the DSPE and a phospholipid bilayer; then constructing a reflection interference spectrum system, and detecting the extracellular vesicles in real time by utilizing optical thickness change caused in the process of separating the extracellular vesicles; according to the method, the extracellular vesicles in various biological liquids can be rapidly and efficiently separated, and real-time concentration and membrane protein expression quantity analysis can be carried out on the separated extracellular vesicles; the invention provides a rapid and low-cost integrated multifunctional analysis method for separation of extracellular vesicles of various body fluids.

To provide a method for preparing extracellular vesicles from culture medium of microalgae, and to provide a composition containing extracellular vesicles obtained by the method.SOLUTION: A preparation method including filtering a culture supernatant of microalgae, and a composition obtained by such a preparation method are provided. Such an extracellular vesicle-containing composition can be used as a composition for maintaining or increasing a fibrous structural protein.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of biological medicine, and particularly relates to an extracellular vesicle drug delivery system, a preparation method thereof and application of the extracellular vesicle drug delivery system in treatment of Alzheimer's disease. According to the invention, one or more of stilbene glucoside, emodin, kaempferol, resveratrol and quercetin are used for regulating and controlling the expression of miRNA (micro Ribonucleic Acid) related to the disease progress in the extracellular vesicles, and the result shows that one or more of miR-let-7c-5p, miR-486-5p, miR-132-3p, miR-160b and miR-129-5p is remarkably up-regulated, and one or more of miR-342-3p, miR-16-5p and miR-125b-5p is remarkably down-regulated. The regulated extracellular vesicles can be used for treating Alzheimer's disease, relieving Tau protein abnormal phosphorylation, reducing amyloid protein beta deposition, inhibiting microglial cell activation, protecting neuronal cells and relieving memory deficits.

The invention belongs to the technical field of biological medicine, and provides an engineered extracellular vesicle, a preparation method, application and a drug for targeted repair of blood brain barrier in order to solve the technical problems that an existing repair tool takes effect slowly and is not enough in specificity, and the engineered extracellular vesicle is loaded with Wnt7a-K190A protein. After entering the brain, the engineered extracellular vesicle can be rapidly and specifically combined with a Gpr124 receptor to promote blood brain barrier repair, and in-vivo experiments verify that the engineered extracellular vesicle can promote blood brain repair, so that the engineered extracellular vesicle can be applied to preparation of targeted blood brain barrier repair drugs, becomes a supplement of existing tools, AAV, LNP and the like, and has a wide application prospect. Particularly, the high requirement on the treatment timeliness of central nervous system (injury irreversibility) diseases is met.

The invention discloses a strain of Weizmannia coagulans and application thereof, the classification name of the strain of Weizmannia coagulans L-B15 is Weizmannia coagulans L-B15, the strain of Weizmannia coagulans L-B15 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation time is January 23, 2025, the preservation number is CGMCC No.33496, and the preservation number is CGMCC No.33496. The strain of Weizmannia coagulans L-B15 is named as Weizmannia coagulans L-B15. The preservation unit address of the bacillus coagulans L-B15 is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and application of the bacillus coagulans L-B15. The extracellular vesicles of the Witzia ciliaris L-B15 disclosed by the invention can be used as a unique active ingredient to be applied to a product for promoting wound healing.

The invention discloses application of pseudo-ginseng external vesicles in preparation of drugs for preventing and treating or improving heart diseases. The plant exovesicle provided by the invention has the advantages of low toxicity, high biocompatibility, biodegradability, no induction of immunogenicity, high yield and large-scale preparation. A large number of experimental studies prove that the pseudo-ginseng external vesicles disclosed by the invention have a remarkable heart disease prevention and treatment effect. Therefore, development and application of the fresh pseudo-ginseng medicine are promoted.

The invention discloses a lactobacillus paracasei extracellular vesicle for relieving ulcerative colitis and application of the lactobacillus paracasei extracellular vesicle, and belongs to the technical field of microbial extracellular vesicles. The invention provides a strain of Lactobacillus paracasei Li-1, and the preservation number of the Lactobacillus paracasei Li-1 is CCTCC (China Center For Type Culture Collection) NO: M 2025435. The invention further provides the extracellular vesicles of the lactobacillus paracasei Li-1, and the extracellular vesicles are extracted from fermentation liquor of the lactobacillus paracasei Li-1. The application of the Lactobacillus paracasei extracellular vesicles in relieving and / or treating ulcerative colitis shows that the Lactobacillus paracasei extracellular vesicles significantly improve the weight change rate, DAI index and colon length of mice, and intervene the progress of ulcerative colitis. The lactobacillus paracasei extracellular vesicles can relieve ulcerative colitis, and a theoretical basis is provided for developing the lactobacillus paracasei extracellular vesicles into microecologics to be applied to the fields of medicines and the like.

The invention discloses application of fissistigma oldhamii outer vesicles in preparation of a medicine for treating inflammation, and belongs to the technical field of food and medical health care products. According to the application disclosed by the invention, the outer vesicles are separated and extracted from fissistigma oldhamii, and experimental verification shows that the fissistigma oldhamii outer vesicles can be used for remarkably inhibiting the proliferation of Raw 264.7 cells, and can be used for remarkably inhibiting the inflammatory response of the Raw 264.7 cells induced by LPS (Lipopolysaccharide). The fissistigma oldhamii outer vesicles provided by the invention are derived from plants, are non-toxic to healthy cells, show an obvious anti-inflammatory function and inhibit cancer cell proliferation, have small side effects, provide a new treatment means for inflammation treatment and cancer prevention, and have great significance in scientific, reasonable and sustainable development and utilization of fissistigma oldhamii resources.

Generally, the present disclosure is directed to methods of producing Resolvin-loaded extracellular vesicles, compositions comprising Resolvin-loaded extracellular vesicles, and methods of treating diseases using such compositions. In some embodiments, methods described herein comprise providing a population of cells, transfecting the population of cells with at least one plasmid vector encoding human cyclooxygenase-2 and 5-lipoxygenase, applying DHA, EP A, and ASA to the transfected population of cells, and isolating the Resolvin-loaded extracellular vesicles from culture media of the transfected population of cells. In some cases, a composition described herein comprises a plurality of extracellular vesicles comprising at least one Resolvin packaged within an interior of the EVs. In some embodiments, upon disposing a plurality of Resolvin-loaded extracellular vesicles within a biological compartment of the patient, inflammation from an inflammatory condition or disease, such as acute lung respiratory distress syndrome, is mitigated or resolved.

The invention provides a medicine for treating acute hepatic failure. The medicine comprises curcumin and extracellular vesicles derived from honey. The invention further provides a preparation method and application of the medicine. According to the invention, the extracellular vesicles are separated from the honey and are proved to have a remarkable anti-inflammatory effect, curcumin is loaded to the extracellular vesicles of the honey, the treatment effect on acute hepatic failure is excellent, and a new effective medicine is provided for clinic.

Provided are an extracellular vesicle, a preparation method, a pharmaceutical composition, and a use. The preparation method comprises: generating an extrusion effect on an extracellular vesicle, so that an exogenous nucleic acid is loaded into the extracellular vesicle; purifying the extracellular vesicle loaded with the exogenous nucleic acid, so as to obtain a highly-expressed extracellular vesicle of the exogenous nucleic acid for diagnosis and treatment; and quantifying the exogenous nucleic acid of the highly-expressed extracellular vesicle of the exogenous nucleic acid for diagnosis and treatment. The exogenous nucleic acid having diagnosis and treatment properties is loaded into the extracellular vesicle on the basis of a high-throughput physical extrusion method. Because the structural size is similar to or slightly smaller than that of the extracellular vesicle, the extrusion effect is generated on the extracellular vesicle, a membrane of the extracellular vesicle is temporarily perforated, and the exogenous nucleic acid is loaded in the extracellular vesicle, so that the in-vitro stability of the nucleic acid is protected. In addition, by performing quantitative design on the exogenous nucleic acid of the highly-expressed extracellular vesicle of the exogenous nucleic acid for diagnosis and treatment, complicated and uncontrollable biochemical processes are avoided, and the quantitative exogenous nucleic acid enters target cells and takes an effect to accurately regulate and control protein expression, thereby increasing the utilization rate of the exogenous nucleic acid.

The current invention relates to isolated Extracellular Vesicles (EVs) wherein said EVs comprise the miRNA markers hsa-miR-125b-5p, hsa-miR-199a-3p, hsa-miR-199b-3p and hsa-miR-16-5p.

An extracellular vesicle-modified metal implant with immunoregulatory and osteoinductive dual functions achieved through use of mussel-derived adhesion peptides and azidylated extracellular vesicles o

The present invention provides a method of recovering an extracellular vesicle from an extracellular vesicle-containing sample. More specifically, the present invention provides a method of recovering the extracellular vesicle that includes separating the extracellular vesicle from the extracellular vesicle-containing sample in the presence of a polymer.

The invention discloses an application of SNF2 protein derived from streptococcus angina extracellular vesicles in gastric cancer prognosis. The chromatin remodeling protein SNF2 derived from S.anginosus EVs can be combined with a transcription factor TEAD1, so that the transcription of the palmitoyl transferase ZDHHC11 is promoted together. Then, the stability of the ZDHHC11 is enhanced by catalyzing palmitoylation of PD-L1, and finally immune escape is induced. In addition, SNF2 also can activate AXL, CTGF, CYR61 and other carcinogenic targets at the downstream of TEAD1, thereby further accelerating the malignant progression of gastric cancer. In an in-vivo experiment, the intragastric administration of the S.anginosus EVs not only promotes the tumor growth of mice, but also significantly inhibits the infiltration of CD8 + T cells. Blocking of ZDHHC11 can effectively reverse immune escape, and has a synergistic effect with an anti-PD-1 therapy, so that the treatment effect is remarkably improved.

The present invention provides extracellular vesicle compositions. Also provided are methods of making an extracellular vesicle loaded scaffold using the extracellular vesicle compositions to increase revascularization. Methods of using the extracellular vesicle compositions and the extracellular vesicle loaded scaffold are provided. The scaffold may increase blood flow and oxygenation of the tissue thereby aiding in treatment of disease and injury.

The present invention provides stabilization of an extracellular vesicle. Specifically, the present invention provides, for example, a method of stabilizing the extracellular vesicle including mixing an extracellular vesicle-containing sample with a saccharide and a chelating agent.