13 results about "Differentiation Inducer" patented technology

The invention discloses a cerium nano-enzyme composite active material and application thereof in hepatic failure treatment, and belongs to the technical field of nano-materials. The invention combines the functions of miR122 and cerium dioxide nano-enzyme, develops a new stem cell differentiation inducer, namely a cerium nano-enzyme composite active material, and deeply studies the potential mechanism of the nano-enzyme material with catalytic activity in regulating stem cell fate, and the cerium dioxide nano-enzyme can be used for delivering miR122 into stem cells after being modified, so that the stem cell differentiation inducer can be used for inducing stem cell differentiation. The miR122 is highly enriched in cells, so that hepatic differentiation is induced. Meanwhile, the catalytic activity of the cerium dioxide nano-enzyme can regulate the ROS steady state in the cells and protect mitochondria from being damaged by ROS induction, so that the mitochondrial function is maintained, the energy requirement in the differentiation process is met, and the method is of great importance to successful differentiation of the stem cells. In order to solve the problem of activity damage in hepatic cell transplantation, a layer of Ce < 4 + >-polyphenol skeleton is coated on the surface of induced hepatic-like cells (iHLCs), so that the function and activity of the hepatic cells after transplantation are further protected, and the in-vivo treatment effect of the hepatic cells is improved.

Provided is reproducible means that enables production of nucleus pulposus progenitor cells (preferably, an active nucleus pulposus progenitor cell phenotype) from desired cells such as terminally differentiated cells and stem cells having pluripotency or multipotency. A nucleus pulposus progenitor cell inducer according to the present invention comprising an effective amount of a gene of Brachyury (T) or a homolog thereof, at least one selected from the group consisting of SRY-box6 (SOX6) or a homolog thereof and Forkhead Box Q1 (FOXQ1) or a homolog thereof, and MYC Proto-Oncogene, BHLH Transcription Factor (cMyc) or a homolog thereof (nucleus pulposus progenitor cell master regulator transcription factor), or a product thereof.

The invention relates to the field of biological medicine, and provides a neuronal cell differentiation culture medium which comprises a basic culture medium, a ROCK pathway inhibitor, a cAMP level enhancer, a neural stem cell differentiation inducer and gamma-aminobutyric acid. The neuronal induced differentiation culture medium provided by the invention is clear in component and convenient to use, and can quickly differentiate the human pluripotent stem cells into neurons with relatively mature shapes and functions. By applying the technical scheme disclosed by the invention, the problems of overlong in-vitro neuronal differentiation period, low purity of obtained neurons, immature functions and the like can be solved, and the method has very high scientific research and clinical application prospects.

The invention provides an embryonic stem cell differentiation technology and application of the embryonic stem cell differentiation technology in screening of unknown risk alternative toxicity of a novel biological breeding product, and Cry1Ab protein is used as a test substance to evaluate the developmental toxicity of the Cry1Ab protein. The EBs are generated through hanging drop culture, and bone differentiation induction substances (beta-glycerophosphate, ascorbic acid and vitamin D3) are added to promote differentiation of the EBs. After bone cell induced differentiation is finished, the differentiation condition of bone cells is observed through alizarin red S dyeing and absorbance value detection, the cell growth condition is observed through cell total protein concentration and alkaline phosphatase activity detection, and then the influence of a test substance on the bone differentiation process is analyzed through the gene expression condition of osteogenic differentiation related markers (Runx2, SPARC and I-type collagen). The invention solves the problem of evaluating the unknown risk of a novel biological breeding product in vitro, creates an embryonic stem cell bone differentiation test technology, and uses the embryonic stem cell bone differentiation test technology as a screening method for the unknown risk alternative toxicity of the novel biological breeding product to be combined with an embryonic stem cell myocardial differentiation experiment for use. And the accuracy of predicating the developmental toxicity of the test substance is improved.

To provide a novel differentiation inducer to skeletal muscle Type I cells, which uses a component that was not previously known to induce differentiation to Type I fibers.SOLUTION: A differentiation inducer to skeletal muscle Type I cells includes R-spondin 3.SELECTED DRAWING: None

The invention relates to the technical field of culture media, in particular to a premixed liquid culture medium capable of being stored for a long time at 4 DEG C. The premixed liquid culture medium comprises a basic culture medium and a special additive and is characterized in that the basic culture medium comprises a carbon source, a nitrogen source, inorganic salt and growth factors; the special additive comprises a growth regulator and a slow release agent, and the special additive further comprises an antioxidant and a directional differentiation inducer. According to the invention, glycerol is added, so that the glycerol can form a protective film on the culture medium, vitamin C is used for preventing the film structure from being oxidized and damaged, normal functions of cells are guaranteed, the slow-release agent is removed under the condition that other components are not changed, and experiments show that the culture medium containing the slow-release agent can be stored for a long time in an environment of 4 DEG C; meanwhile, the activity of animal and plant cells is not influenced.

The application discloses a cerium nanoscale enzyme composite active material and application thereof in liver failure treatment, and belongs to the technical field of nanomaterials. The application combines miR122 and cerium dioxide nanoscale enzyme functions, develops a new stem cell differentiation inducer, i.e., a cerium nanoscale enzyme composite active material, and deeply studies the potential mechanism of catalytically active nanoscale enzyme material in regulating stem cell fate. After modification, the cerium dioxide nanoscale enzyme can be used for delivering miR122 into stem cells, so that miR122 is highly enriched in cells, thereby inducing liver differentiation. Meanwhile, the catalytic activity of the cerium dioxide nanoscale enzyme can regulate intracellular ROS homeostasis, protect mitochondria from ROS-induced damage, thereby maintaining mitochondrial function and meeting the energy demand in the differentiation process, which is crucial for the successful differentiation of stem cells. In order to solve the problem of impaired viability in hepatocyte transplantation, it is proposed to coat a layer of Ce 4+ -polyphenol skeleton on the surface of induced hepatocyte-like cells (iHLCs), further protecting the function and viability of hepatocytes after transplantation, and improving the in-vivo treatment effect.

To provide a macrophage production method capable of yielding macrophages that have a prolonged survival rate, allow for freezing preservation, and offer versatility as a tool in scientific studies.SOLUTION: A method for producing macrophages comprises a culture step for culturing hematopoietic precursor cells in the presence of a TREM2 signal activator.SELECTED DRAWING: Figure 1

The objective is to provide a previously unknown adipocyte differentiation inducer. [Solution] A fat cell differentiation inducer containing sesamolinol as an active ingredient.

The invention discloses a gold nanocluster composite material functionalized microsphere and application thereof, and belongs to the technical field of biomedicine. According to the invention, the gold nanocluster loaded microRNA is used as a stem cell differentiation inducer, and is introduced into the GelMA / HAMA gel for constructing the functional microspheres (ARGH microgel), so that necessary conditions are provided for the growth and differentiation of stem cells. The ARGH microspheres provide sites for stem cell adhesion and growth, and meanwhile, surface stem cells are regulated and controlled to be differentiated into hepatocytes. A cell-loaded ARGH microsphere secretion group shows angiogenesis promoting and immunoregulation activity, and meanwhile, cells on the surfaces of different ARGH microspheres are aggregated through intercellular interaction and are self-assembled into a micro-tissue with a larger size. The micro liver tissue is transplanted into the liver failure mouse body, the function and the treatment effect of the micro liver tissue are further evaluated, and the result shows that in two mouse liver failure models, micro liver tissue transplantation treatment can effectively recover the liver function and promote liver regeneration and repair.

A method and composition for treating cancer through induction of tumor cell differentiation is disclosed. Cancer cells are converted into terminally differentiated, non-proliferating cell types using specific differentiation-inducing agents, including thyroid hormone analogs such as T2 and RT3. The approach avoids the cytotoxic effects and side effects associated with conventional chemotherapy while potentially providing curative treatment. Pharmaceutical compositions include topical formulations for skin cancer treatment and various delivery systems for systemic administration. In vitro screening methods for identifying differentiation-inducing agents are also provided.

Provided is reproducible means that enables the production of an active nucleus pulposus cell phenotype from desired cells such as terminally differentiated cells or pluripotent or multipotent stem cells. Provided is a differentiation inducer containing an effective amount of a gene of at least two transcription factors selected from the group consisting of Brachyury (T), SRY-box6 (SOX6), and Forkhead Box Q1 (FOXQ1), or homologs thereof (nucleus pulposus cell master regulator transcription factor), or a product thereof.

To provide a method for producing retinal pigment epithelial cells.SOLUTION: A method of producing retinal pigment epithelium (RPE) cells, comprising the steps of: (a) culturing a cell population of undifferentiated human pluripotent stem cells on an adherent surface selected from the group consisting of laminin, fibronectin, vitronectin, collagen I and collagen IV in a medium comprising nicotinamide as a differentiation-inducing agent and lacking activin A under feeder cell-free conditions to obtain differentiated cells; (b) culturing the differentiated cells on the adherent surface in a medium comprising nicotinamide and one or more members of the TGF β superfamily selected from the group consisting of TGF β 1, TGF β 3, and activin A to obtain RPE cells, wherein at least 50% of the cells in the population of cells are Oct4 + TRA-1-60 +.SELECTED DRAWING: Figure 1