The invention provides a blood-based noninvasive
early lung cancer detection method, which investigates a panel of miRNA levels in a blood or
plasma sample. The panel of miRNA includes miR-17, miR-21, miR-24, miR-106a, miR-125b, miR-128, miR-155, miR-182, miR-183, miR-197, miR-199b, miR-203, miR-205, miR-210, miR-221, and a combination thereof. Preferably, the panel of miRNA may include miR-21, miR-128, miR-155, miR-182, miR-183, and miR-197. The inventive method can not only detect stage I
lung cancer patients with high accuracy, but also differentiate between all stages of
lung cancer patients and
lung cancer-free individuals, metastatic and non-metastatic
lung cancer patients and monitor the significant changes of miRNA levels during
chemotherapy.