Application of serum protein marker composition in screening and diagnosis and treatment of lung cancer

A protein, lung cancer technology, applied in disease diagnosis, biomaterial analysis, measurement devices, etc., can solve problems such as limited sensitivity and specificity

Inactive Publication Date: 2018-12-25
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these tumor markers alone have limited sensitivity and specificity

Method used

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  • Application of serum protein marker composition in screening and diagnosis and treatment of lung cancer
  • Application of serum protein marker composition in screening and diagnosis and treatment of lung cancer
  • Application of serum protein marker composition in screening and diagnosis and treatment of lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Example 1: Sensitivity and specificity of protein combinations in the detection of serum samples from lung cancer patients

[0081] For 174 cases of lung cancer patients (mainly non-small cell lung cancer, regardless of whether it is early stage) and 133 cases of normal human serum samples, ELISA method was used to detect CYFRA21-1, NSE, IL-2, IL-8, IFN-γ, MIP The levels of seven proteins -1β and VEGF-A were analyzed.

[0082] The experimental results are shown in Table 1, Table 2 and Figure 1A - Figure 1G shown.

[0083] Table 1: Sensitivity and specificity of individual proteins in lung cancer

[0084]

[0085] Note: All samples are valid samples.

[0086] Table 2: Sensitivity and specificity of protein combinations in lung cancer

[0087]

[0088] Note: 7 out of 2 positive means 2 out of 7 antibodies are positive; 6 out of 2 positive means 2 out of 6 antibodies are positive; 5 out of 2 positive means 2 out of 5 antibodies are positive; 4 out of 2 Posi...

Embodiment 2

[0090] Example 2: Sensitivity of protein combinations in the detection of early lung cancer serum samples

[0091] For 29 cases of early (stage I, stage II) lung cancer (non-small cell lung cancer), seven CYFRA21-1, NSE, IL-2, IL-8, IFN-γ, MIP-1β and VEGF-A were detected by ELISA protein levels and analyzed.

[0092] The experimental results are shown in Table 3 and Table 4.

[0093] Table 3: Sensitivity of single proteins in early lung cancer

[0094] protein name

Number of positive cases

sensitivity

CYFRA21-1

16

55.17％

NSE

6

20.69％

IL-2

0

0.00％

IL-8

9

31.03％

IFN-γ

24

82.76％

MIP-1β

18

62.07％

VEGF-A

19

65.52％

[0095] All samples are valid samples

[0096] Table 4: Sensitivity of protein combinations in early lung cancer

[0097]

[0098]

[0099] Note: 7 out of 2 positive means 2 out of 7 antibodies are positive; 6 out of 2 positive means 2 out of 6 ant...

Embodiment 3

[0101] Example 3: Changes in protein combinations in serum samples of lung cancer patients before and after surgery

[0102]For the serum samples of 20 patients with lung cancer (non-small cell lung cancer) before and after surgery, the levels of six proteins, CYFRA21-1, NSE, IL-2, IL-8, IFN-γ and VEGF-A, were detected by ELISA .

[0103] result Figure 2A-Figure 2D And as shown in Table 5.

[0104] Table 5: Decrease of single protein serum level in patients with lung cancer before and after surgery

[0105]

[0106]

[0107] Note: All samples are valid samples.

[0108] The results showed that the serum levels of CYFRA21-1 and NSE proteins decreased significantly after surgery. Combination analysis of CYFRA21-1, NSE, IL-2, and IFN-γ and other proteins showed that the combination of 3 selections 1 and 4 selections 1 combination detected 95% and 100% of colorectal cancer patients with decreased serum levels after surgery. %, see Table 6 for details.

[0109] Table...

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Abstract

The invention belongs to the technical field of molecular biology and clinical detection, and relates to a protein composition containing any two, three, four, five, six or seven selected from the group consisting of CYFRA21-1, NSE, IL-2, IL-8, IFN-gamma, MIP-1beta and VEGF-A. The invention also relates to a ligand composition that binds to the protein specificity in the protein composition, and application of the protein composition or ligand composition in the preparation of a kit for screening, auxiliary diagnosis, treatment monitoring and prognosis of lung cancer. The invention can be usedfor screening, auxiliary diagnosis, treatment monitoring and prognosis judgment of lung cancer, especially screening and auxiliary diagnosis of early lung cancer, which has high sensitivity and highspecificity, and is superior to the detection of single protein markers and clinical commonly-used serum markers CYFRA21-1 and NSE.

Description

technical field [0001] The invention belongs to the technical field of molecular biology and clinical detection, in particular, it relates to a marker comprising seven proteins selected from CYFRA21-1, NSE, IL-2, IL-8, IFN-γ, MIP-1β and VEGF-A Combination, and the application of the combination of markers in the preparation of serum marker kits for lung cancer screening, auxiliary diagnosis, treatment monitoring and prognosis judgment. Background technique [0002] Lung cancer is the most common malignant tumor in all countries in the world today. The incidence and mortality of lung cancer in men account for the first of all malignant tumors, and the incidence and mortality of lung cancer in women occupy the second place. In my country, according to the latest data from the National Cancer Center, there were 704,800 new cases of lung cancer in China in 2012, with an incidence rate of 52.06 / 10 6 ;Death cases were 569,400, and the death rate was 42.05 / 10 6 , ranking first am...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57423G01N2800/60
Inventor 王明荣郝佳洁常晨张钰徐昕蔡岩
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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