Construction method of ELISA detection kit, kit and application

Abstract

The invention belongs to the technical field of markers and detection thereof. The invention discloses a construction method of an ELISA detection kit, a kit and application. The method includes: preparing 1L of a coating liquid, preparing 1L of a 10XPBST washing liquid, preparing 1L of a 1X PBST washing liquid, preparing a confining liquid, preparing an antibody diluent, preparing McIvain's buffer, preparing a developing liquid and preparing a 0.5M phosphate buffer solution. According to the invention, EGFR is verified to be capable of causing immunoreaction of an organism and enabling the organism to generate a corresponding autoantibody, is low in cost, high in specificity, strong in sensitivity, high in accuracy and noninvasive, can be used as a diagnostic biomarker for early lung cancer, is used for distinguishing lung cancer patients from normal people, and is used for identifying different types of lung cancer patients from a normal control group; the EGFR autoantibody also hasa diagnostic value for early lung cancer, and can be combined with a clinical indicator CEA to improve the lung cancer diagnosis accuracy.

Description

technical field [0001] The invention belongs to the technical field of markers for lung cancer diagnosis and detection thereof, and in particular relates to a construction method, kit and application of an ELISA detection kit. Background technique [0002] At present, lung cancer is one of the most common malignant tumors with the highest morbidity and mortality in the world. About 1.8 million new lung cancer cases occur every year, accounting for about 13% of all cancer diagnoses. In recent years, the incidence rate has shown an increasing trend. The onset of lung cancer is hidden and there are no obvious clinical symptoms in the early stage. The course of the disease develops rapidly. It is usually diagnosed in the middle and late stages and the best time for treatment is missed, and the prognosis is poor. At present, the clinical diagnosis of lung cancer is mainly combined with imaging and "gold standard" pathological cell technology. Due to the shortcomings of low early ...

AI Technical Summary

Problems solved by technology

[0008] Aiming at the problems existing in the prior art, the present invention provides a construction method, kit and application of an ELISA detection kit, aiming at solving the problem of unsatisfactory existing lung cancer tumor markers, improving the sensitivity of early diagnosis of lung cancer, Specificity and Accuracy

Images