325 results about "Immunoresponse" patented technology

The present invention relates, in general, to tissue decellularization and, in particular to a method of treating tissues, for example, heart valves, tendons and ligaments, so as to render them acellular and thereby limit mineralization and/or immunoreactivity upon implementation in vivo.

Provided are methods of separating an immunoreactive compound from at least one immaterial component, using a simulated moving bed ("SMB") system and a SMB apparatus for use in these methods. Also provided are purified immunoreactive compounds prepared using the SMB methods and apparatus and methods of treatment with the purified immunoreactive compounds.

Normal tissue complications limit curative broadbeam radiotherapy to tumors including lung cancer. Radiation retinitis causing blindness limits quality of life and long term survival for patients with ocular melanoma. This invention pertains to alternative, normal tissue sparing 100 to 1,000 Gy microbeam radiations with least normal tissue complications and concomitant radio-immunotherapy by innate immune response of epidermis and dermis to low dose radiation with 50 kV X-rays. Total body skin radiation with former airport passenger screening machines with 50 kV X-ray is disclosed. Microbeams are generated without contaminating scatter and neutron radiations from collinear gamma ray and electron beam produced by inverse Compton interaction with high energy laser and electron beam and from proton and carbon ion beams in tissue equivalent cylindrical collimators. Extracorporeal immunotherapy and chemotherapy and apheresis of mutated subcellular particles released into circulation in response to cancer-therapies are by clinical continuous flow ultracentrifugation combined chromatography.

The invention discloses a method for quantitative detection of biological molecules capable of being metabolized to generate H2O2 in serum by means of a ratiometric fluorescent probe. The method includes firstly preparing copper-nitrogen codoped carbon dots Cu-CDs; then reacting corresponding oxidase and the biological molecules capable of being metabolized to generate H2O2 to generate H2O2; catalyzing the H2O2 and a substrate that is o-phenylenediamine with horseradish peroxidase to generate an oxidation product DAP having yellow fluorescence; then adding the Cu-CDs into the DAP; allowing the DAP and the Cu-CDs to form ratiometric fluorescence, with the ratio I<572>/I<460> of fluorescence intensities of the DAP and the Cu-CDs being in a linear relationship with the concentration of a substance to be detected; and performing quantitative assay of the biological molecules in the serum according to the ratio of fluorescence intensities. The method is high in sensitivity and simple and convenient in detection, and has high selectivity and high affinity of immunoreactions.

The invention belongs to the technical field of biology and specifically relates to a preparation method and an application of a nano antibody capable of specially binding with an anti-deoxynivalenol antibody. The amino acid sequence of the nano antibody is SEQ ID No. 1. The invention also relates to a nucleotide for encoding amino acid. The nano antibody is capable of taking the place of the expensive high-toxicity DON standard substances, and can be applied to the immunological detection of the DON as a competitive antigen or a solid-phase envelope antigen; the nano antibody has immunoreaction characteristic similar to that of natural DON molecule, and is excellent in effect. Compared with the traditional antigen mimic epitopes based on polypeptides and the traditional anti-idiotype antibodies based on IgG, the nano antibody has the characteristics of more stable structure, acid-alkali resistance, high temperature resistance, high detection sensitivity and the like, and therefore, the immunodetection stability of the nano antibody is greatly improved, and meanwhile, the endurance capacity of the nano antibody to the environment is also improved.

The invention relates to a relaxation time immunosensing analysis method based on magnetic separation. The method comprises the following steps: selecting two magnetic beads (one can be quickly separated and the other cannot be separated) different in saturated magnetization intensity and remarkably different in separation speed in the same magnetic field, so that the magnetic beads can be separated in the magnetic field; coupling the magnetic beads to an antibody used for identifying different sites of the same target object respectively to prepare immunomagnetic beads; producing an immunoreaction of the immunomagnetic beads and a to-be-detected sample; performing magnetic separation on a mixed system, measuring transverse relaxation time for supernatant liquor subjected to magnetic separation, and determining the concentration of biomacromolecules in the to-be-detected sample according to change of the transverse relaxation time. The immunomagnetic beads different in saturated magnetization intensity are different in separation speed in the same magnetic field, the magnetic separation is combined with magnetic relaxation time analysis, the reaction time only needs 30 minutes, and the method can be used for quickly detecting bacteria, viruses and proteins and has a very good application prospect in an aspect of biomarker detection.

The invention relates to a bursin extracting method and its application to curing disease and immunity, having important value in application in the aspects of heightening organismal immunity and acting as immunoenhancer, heightening effect of vaccine, etc., and able to heighten body fluid and cell immune functions of mammal at the same time. It can be used to prevent and cure infectious diseases and young animal diseases singly or together with other drugs such as antivirus and antibacterial drugs or immunomodulators, also be applied to animal vaccine as adjuvant or immunoenhancer to strengthen the disease-resistant ability and immunoresponse ability to peculiar antigens, thus heightening the immune effect.

The invention provides a clenbuterol (CLB) magnetic particle separation enzyme-linked immunosorbent assay (ELISA) method, belonging to the technical field of food safety immunodetection. The method comprises the following steps: the immunodetection principle of the competition method is adopted to connect CLB with bio-enzyme and prepare an enzyme labeled antigen reagent, antibody against fluorescein isothiocyanate (FITC) is absorbed on the surface of magnetic particles to prepare a magnetic separation reagent, FITC is connected with the antibody against CLB to prepare an antibody reagent; CLB in a sample and enzyme-labeled CLB compete to combine with a small quantity of FITC-labeled antibody against CLB and form an antigen-antibody composite; after the magnetic separation reagent is added, the antibody against FITC connected with the surface of magnetic particles captures the composite on the surface of magnetic particles; and washing, and finally adding substrate to detect. The invention has the following advantages: (1) magnetic particles replace the traditional ELISA plate to be used as solid carrier and ensure that the immunoreaction is performed more fully and fast under the near-liquidus condition; compared with the traditional ELISA method, the method is characterized by high specificity and good repeatability; and (2) the one-step competition method principle is adopted, thus the detection time is short.

The invention discloses an electrochemical immunoassay method based on Au-PB-SiO2 composite nano-particles. The detection method adopts a double antibody sandwich method, allows neuron-specific enolase antibodies solid-supported on an electrode surface to perform an immunoreaction with neuron-specific enolase in a sample solution, and to combine with a co-coupled substance of Au-PB-SiO2 composite nano-particles and neuron-specific enolase antibodies. Based on the electrochemical activity of Au-PB-SiO2 composite nano-particles, the detected peak current value of a cyclic voltammetry reduction peak is positively correlated with the concentration of the neuron-specific enolase, and thus the concentration of the neuron-specific enolase in the sample to be detected can be detected. The electrochemical immunoassay method provided by the invention has a corresponding linear range of 0.25-500 ng/ml, and a lower detection limit of 0.08 ng/ml, has good specificity and high sensitivity, and has important significance for the diagnosis of small cell lung cancer (SCLC).

The invention provides an enzyme linked immunosorbent assay (ELISA) kit for detecting progesterone and a detection method thereof. The kit is sensitive, accurate, quick in detection, is simple in operation, is strong in specificity and is suitable for detection of samples in large amount. The kit includes: an ELISA plate coated by a progesterone antigen, a progesterone standard sample, a progesterone antibody operating fluid, a progesterone enzyme label second antibody operating fluid, a substrate liquid A, a substrate liquid B, a stop buffer liquid, a concentrated diluent and a concentrated washing liquid. The principle of the ELISA kit for detecting progesterone is solid-phase indirect competitive ELISA. In the detection method, an extracted sample, the progesterone enzyme label second antibody operating fluid and the progesterone antibody operating fluid are added to corresponding microholes in the ELISA plate. After incubation for a certain time, the ELISA plate is washed and is added with the substrate liquid A and the substrate liquid B, and then a color developing agent develops a blue color under effect of enzymes. After the stop buffer liquid added, the color is turned into yellow from blue. An inversely proportional relationship is formed between the depth of the developed color and the content of the progesterone in the standard sample or the sample. The method can be directly used for detecting residual amount of the progesterone in chicken tissue.

Immunoprotective primary mesenchymal stems cells (IP-MSC) which episomally express multiple immunoreactive polypeptides that specifically target a pathogen (e.g., an infectious species of virus, bacterium, or parasite) or toxin are described herein. The IP-MSC express two or more (e.g., 2 to about 100) immunoreactive polypeptides (e.g., full antibodies, single-chain antibodies (ScFV), Fab or F(ab)₂ antibody fragments, diabodies, tribodies, and the like), and optionally one or more other immunomodulating polypeptides, e.g., a cytokine such as an interleukin (e.g., IL-2, IL-4, IL-6, IL-7, IL-9, and IL-12), an interferon (e.g., IFNα, IFNβ, or IFNω), and the like, which can enhance the effectiveness of the immunoreactive polypeptides.

The invention discloses portable electrochemical quantitative immunochromatography filtering paper as well as test paper and application of the electrochemical quantitative immunochromatography filtering paper. The immunochromatography filtering paper is formed by arranging a hydrophilic channel on hydrophilic printing filtering paper; a sample feeding region is arranged at one end of the channel and a water absorption region is arranged at the other end of the channel; an electrochemical detection region is arranged between the sample feeding region and the water absorption region; water absorption paper is arranged in the water absorption region; the electrochemical detection region is coated with an antigen or antibody for detecting a target substance; the portable electrochemical quantitative immunochromatography filtering paper is manufactured by immunochromatography filtering paper, a conductive film and a bottom plate; the test paper can be used for quantitatively analyzing immunoreaction and eliminating the non-specific interferences, and has the advantages of accurate result, accuracy in measurement and the like.

The invention belongs to the technical field of animal epidemic disease serological diagnosis, and relates to an enzyme-linked immunosorbent assay vector and a kit for detecting avian leukosis P27. The avian leukemia virus (P27) enzyme linked immunosorbent assay kit comprises a 96 hole elisa plate coating a avian leukosis P27 polyclonal antibody, P27 monoclonal antibody marked by alkaline phosphatase, a substrate solution, a stop solution and a cleaning solution. The vian leukosis P27 polyclonal antibody and the P27 monoclonal antibody marked by alkaline phosphatase effectively improve the sensitivity, the specificity and the stability of the detection. The invention provides an efficient and sensitive ELISA (enzyme linked immunosorbent assay) detection kit for sifting out and purifying avian leukosis positive chickens and cultivating new species with heredity resistance, and the kit is low in cost and simple and convenient to operate, and is applicable to promotion in animal husbandry.

The present invention relates to a method for enhancing immunoreactivity of a tissue or cell sample fixed in a fixing medium, a target retrieval composition and its use. The method comprises providing a carrier in a horizontal position, said carrier having thereon a tissue or cell sample, said tissue or cell sample being on top of the carrier; contacting substantially the tissue or cell sample side of the carrier with a buffered target retrieval solution, wherein the target retrieval solution remains otherwise exposed to the environment; heating the tissue or cell sample and the target retrieval solution to a temperature above 100° C. The invention furthermore concerns the automated immunohistochemical staining of said samples, in particular the reactivation of antigens masked by fixation.

The invention discloses a photonic crystal ordered structure fluorescence enhancement effect-based high-sensitivity immunochromatographic method which comprises the following steps: self-assembling SiO2 particles into a chromatographic channel of a SiO2 photonic crystal film, immobilizing a capture antibody corresponding to an analyte in a detection region in the channel, and labelling a tracked antibody with fluorescent substances, performing immunoreaction to form a capture antibody-analyte-tracked antibody sandwich complex on the film, detecting by utilizing the fluorescent substances labelled on the tracked antibody, and amplifying a fluorescent signal through the optical effect of an ordered structure of the photonic crystal. By using the photonic crystal film as a substrate of immunochromatography, the cost is low, the sensitivity of the immunochromatographic technology can be obviously improved, and the detection limit is reduced.