Bursopoietin extracting method and its use in disease treating and immune

A technology of bursalin and disease treatment, which is applied to the extraction method and its application in disease treatment and immunization, can solve the problems of limited application, high cost, and difficult storage, and achieve no drug residue, stable properties, and easy absorption effect

Inactive Publication Date: 2004-09-15
王爱华 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] the above The immune enhancement effect is good, but due to High cost and difficult to store However, its application in veterinary clinics is greatly limited.

Method used

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  • Bursopoietin extracting method and its use in disease treating and immune
  • Bursopoietin extracting method and its use in disease treating and immune
  • Bursopoietin extracting method and its use in disease treating and immune

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Experimental program
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Embodiment Construction

[0043] Extraction process of the present invention is:

[0044] Take 80g of healthy bursa, add an appropriate amount of 1N glacial acetic acid application solution, and homogenize at 20 000r / min for 3min to make it into a paste. Shake on a horizontal shaker for 1.5h to 2h, centrifuge at 4500r / min at 4°C for 60min, take the supernatant, put it in a boiling water bath for 20min, centrifuge again, take the supernatant and filter. The filtrate was concentrated to about 40ml under reduced pressure with a rotary evaporator between 60°C and 70°C. The concentrated solution was centrifuged at 20,000 g at a high speed for 30 min at 4°C to obtain about 20 ml of the supernatant, which was the crude extract. Pass the crude extract through a G-50 Sephadex column, elute with 1N acetic acid, collect CuSO 4 React positive fraction with 7.5% NaHCO 3 Adjust the pH value to neutral, which is the tissue extract—bursal fasci.

[0045] The present invention is mainly used in:

[0046] 1. Used a...

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Abstract

The invention relates to a bursin extracting method and its application to curing disease and immunity, having important value in application in the aspects of heightening organismal immunity and acting as immunoenhancer, heightening effect of vaccine, etc., and able to heighten body fluid and cell immune functions of mammal at the same time. It can be used to prevent and cure infectious diseases and young animal diseases singly or together with other drugs such as antivirus and antibacterial drugs or immunomodulators, also be applied to animal vaccine as adjuvant or immunoenhancer to strengthen the disease-resistant ability and immunoresponse ability to peculiar antigens, thus heightening the immune effect.

Description

1. Technical field: [0001] The invention relates to an immune preparation for livestock and poultry, in particular to a method for extracting bursal and its application in disease treatment and immunity. 2. Background technology: [0002] The occurrence of infectious diseases, especially viral infectious diseases, is an important factor affecting the development of animal husbandry. After most pathogenic microorganisms invade the body, they can cause damage to the immune organs, weaken the body's immune function, weaken the disease resistance, and further develop the disease. , At the same time, it causes secondary infection and immune failure, causing huge losses to the aquaculture industry. Relying solely on vaccine immunization, on the one hand, due to the failure of immunity caused by various reasons, on the other hand, due to the limitation of the immune protection rate of the vaccine itself, it is difficult to completely prevent the occurrence of infectious diseases. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/00A61K9/20A61K38/06A61P31/12A61P37/04C07K5/08
Inventor 王爱华靳亚平周乐李键
Owner 王爱华
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