58results about How to "Raise the level of immune response" patented technology

The invention relates to a bursin extracting method and its application to curing disease and immunity, having important value in application in the aspects of heightening organismal immunity and acting as immunoenhancer, heightening effect of vaccine, etc., and able to heighten body fluid and cell immune functions of mammal at the same time. It can be used to prevent and cure infectious diseases and young animal diseases singly or together with other drugs such as antivirus and antibacterial drugs or immunomodulators, also be applied to animal vaccine as adjuvant or immunoenhancer to strengthen the disease-resistant ability and immunoresponse ability to peculiar antigens, thus heightening the immune effect.

The invention discloses a porcine circovirus 2 (PCV 2) Cap-cell-penetrating peptide fusion protein gene with high expression and application thereof. Coding genes of the fusion gene refer to any kindof genes in (1)-(3): (1) Cap genes of fused cell-penetrating peptide TAT gene sequence; (2) Cap genes of fused cell-penetrating peptide ppTG20 gene sequence; and (3) Cap genes of fused bee signal peptide HBM gene sequence. Study of the research group discovers a DNA vaccine prepared by mixing a cell-penetrating peptide and pVAX-Cap plasmid is capable of improving the in-vivo immune response levelof mice. According to the study, the TAT, ppTG20, HBM and PCV2 Cap protein N end are fused, the recombinant baculovirus is successfully constructed, effective expression of the Cap proteins is realized, and a foundation is laid for researching genetically engineered vaccine PCV 2.

An anti-infectious immunopotentiator for pig, ox, yak, etc is prepared through artificially synthesizing the CpG oligonucleotide sequce able to excite the reproductive activity of immune cell, preparing the chitosan nanoparticles from deacetyl chitosan, and using said chitosan nanoparticles for molecular packaging of CpG oligonucleotide. It can be used to immunize the experimental animal by intramuscular injection or oral applialtion.

The invention relates to a snail feed nutrient. The snail feed nutrient comprises the following components in part by weight: 3-5 parts of amino acid, 2-4 parts of lecithin, 4-6 parts of vitamin, 2-4 parts of sodium selenite, 5-7 parts of ferrous sulfate, 15-22 parts of soybean protein isolate powder, 18-20 parts of earthworm powder, 8-15 parts of eggshell powder, 10-20 parts of kelp powder and 2-4 parts of composite bacterial agent. The snail feed nutrient provided by the invention has the advantages of improving the appetite, promoting the growth of an animal, promoting the feed digestion, enhancing immune function of the organism, preventing and treating snail rotten foot diseases and shell top dropping diseases as well as increasing the commodity value, and has the characteristics of no toxic and side effect and no drug residue. The snail feed nutrient provided by the invention is a green and safe feed nutrient.

The invention discloses a crucian carp feed containing a compound Chinese herbal medicine immunity enhancer. The crucian carp feed comprises the raw materials of corn flour, wheat bran, bean pulp, wheat, bagasse, fish bone powder, vinegar dregs, corn straw, rice bran, rapeseed dregs, silkworm chrysalis meal, Chinese chives, spinach leaves, the compound Chinese herbal medicine immunity enhancer and phagostimulant. The compound Chinese herbal medicine immunity enhancer is prepared from folium cortex eucommiae, isatis roots, honeysuckle flowers, dried tangerine peel, folium artemisiae argyi, hawthorn fruit, Chinese mugwort, tea, milkvetch roots, lignum sappan, common andrographis herb and cortex cinnamomi according to certain proportions. According to the crucian carp feed containing the compound Chinese herbal medicine immunity enhancer, raw material matching and proportioning of the crucian carp feed are optimized, the feed is high in use ratio and good in palatability, the weight increasing speed of crucian carps is significantly increased, and the added compound Chinese herbal medicine immunity enhancer can effectively enhance the immunity function of the crucian carps and improve the disease resistance of the crucian carps, so that the survival rate of the bred crucian carps is greatly increased. Therefore, application of antibiotics can be reduced, the yield of the crucian carps can be increased, the quality of the crucian carps can be improved, and important industrial value is achieved.

The invention relates to the field of veterinary biological products and is aimed at providing a veterinary vaccine immunologic adjuvant as well as a preparation and application method thereof. The adjuvant is prepared by mixing water-soluble calcium carbonate, polysorbate 80, 2-phenoxy ethanol, chitosan oligosaccharide, xanthan gum and deionized water, wherein the weight ratio of the water-soluble calcium carbonate to deionized water is 0.001-0.01%; the volume ratio of the polysorbate 80 to deionized water is 1-10%; the final concentration of the 2-phenoxy ethanol is 0.5-2.5% (volume ratio); the final concentration of the chitosan oligosaccharide is 0.5-2% (weight ratio); the final concentration of the xanthan gum is 0.1-2% (weight ratio). The adjuvant provided by the invention can enhance the identification ability of lymphocytes for the A, B and H antigens, promote the combination of multiple antigens with an antibody and enhance the immune response level and antigen presentation ability. The adjuvant is kept in a steady state at low temperature and room temperature; due to the antibacterial ability, secondary infection after immunization is avoided. The adjuvant is easy to prepare and thus can be stored for long time at room temperature. The cost of raw materials is low, and the adjuvant is suitable for large-area promotion and application.

The invention provides an immunoenhancer, a swine foot and mouth disease vaccine composition containing the immunoenhancer and application. The immunoenhancer is prepared from three swine foot and mouth disease virus CTL epitope polypeptides, i.e., one or a random combination of two or more of a polypeptide shown as a sequence 1 in a sequence table, a polypeptide shown as a sequence 2 in the sequence table and a polypeptide shown as a sequence 3 in the sequence table. The swine foot and mouth disease vaccine composition provided by the invention is prepared from the immunoenhancer and a swinefoot and mouth disease antigen. The immunoenhancer provided by the invention can be prepared in a large batch through a solid-phase chemical synthesis method and is mixed with an inactivation antigenaccording to a certain ratio, so that the vaccine composition can be produced and prepared in a large scale; the vaccine composition can be used for inducing organisms to generate a body fluid immuneresponse level and also can be used for enhancing a cell immune response level of the organisms and obtaining a relatively good immune effect. The immunoenhancer provided by the invention is safe andreliable and has a wide application prospect.

The invention discloses peptide feed additive used to modulate the immune functions of seawater fish. The peptide feed additive consists of three or more than three types in peptide which is produced by enzymatic hydrolysis of cod fish, skin and internal organs with the molecular weight ranges respectively 100Da to 500Da, 500Da to 1500Da, 1500Da to 3000Da, 3000Da to 5000Da, and 5000Da to 8000Da. 1 percent to 5 percent of immune modulator of the invention is added into feed, which can obviously improve the level of IgM and muramidase in the serum of the seawater fish; the immune modulator of the invention can also improve the liver SOD level of the cultivated fish, thereby improving the oxidation resistant capability of the cultivated fish. The immune modulating peptide feed additive of the invention can also obviously improve the daily weight gain of the fish and reduce the liver index. The peptide feed additive used to modulate the immune functions of the seawater fish is safe without public harm, and the fish cultivated by the additive meet the green food sanitation requirements.

The invention discloses an immune-enhanced recombinant PRRSV (Porcine Reproductive and Respiratory Syndrome Virus)-like particle subunit vaccine. The immune-enhanced recombinant PRRSV virus-like particle subunit vaccine consists of PRRSV-like particles and a compound immunologic adjuvant. The immune-enhanced recombinant PRRSV-like particle subunit vaccine disclosed by the invention has the beneficial effects that by the means of gene engineering, PRRSV GP5-M gene is modified, and the PRRSV-like particles with high immunogenicity are prepared by constructing rhabdovirus expression vectors. In addition, by improvement on the immunologic adjuvant, the immune-enhanced recombinant PRRSV-like particle subunit vaccine is obtained and the subunit vaccine has a better immune effect.

An anti-infectious immunopotentiator for animals is prepared through cloning the interleukin-4 gene of Tibetan pig, configuring its eukaryon expression plasmid VTPIL-4, preparing chitosan nanoparticles from deacetyl chitosan, and using said chitosan nanoparticles for molecular packaging of plasmid VTPIL-4. It can be used for immunizing experimental animal by intramuscular injection.

The invention discloses a complex immunity adjuvant. The adjuvant is prepared from the following components in percentage by weight: 1.5 to 3.5 percent of horttuyia cordala thunb polysaccharide, 0.2 to 0.55 percent of alanine, 0.8 to 2.2 percent of ginkgo leaf flavonoid, 5 to 8 percent of polyethylene castor oil, 5 to 8 percent of Span 80, 2.5 to 5 percent of polyethylene glycol, 6 to 10 percent of squalene, 30 to 35 percent of soybean oil for injection and 35 to 50 percent of water for injection, and the sum of the percentage by weight of the various components is 100 percent. The complex immunity adjuvant is safe and effective, can effectively enhance the immunostimulation capacity of PRRSV vaccines, and is suitable for immunoprophylaxis of porcine reproductive and respiratory syndrome (PRRS).

The invention discloses recombinant serum amyloid A capable of enhancing immune response of crassostrea gigas. The recombinant serum amyloid A is characterized that the amino acid sequence is represented by SEQ ID NO. 1. Gene expression of cytokines CgIL17-1, CgIL17-5, and CgTNF in blood lymphocytes can be remarkably promoted inside and outside bodies of the crassostrea gigas, the immune responselevel of the crassostrea gigas can be enhanced, and the recombinant serum amyloid A has application value in preparation of novel immune preparations and other drugs for shellfishes, especially breeding shellfishes.

The invention provides a polylactic acid-cladded protein peptide vaccine, a protein peptide vaccine carrying system for a protamine-cladded polylactic acid nanocapsule and a preparing method of the carrying system. Ovalbumin (OVA) is used as a model protein peptide vaccine, and an OVA solution is dispersed in a PLA-containing methylene dichloride organic phase by a probe ultrasonography method to form a primary emulsion. The primary emulsion is added into a polyvinyl alcohol containing external aqueous phase and treated by probe ultrasonography to form a multiple emulsion. A cured OVA/PLA microcapsule is obtained after the methylene dichloride organic phase is fully volatilized through agitating. Protamine (PS) is added into an OVA/PLA microcapsule suspension and agitated to obtain an OVA/PLA/PS microcapsule. The OVA/PLA/PS microcapsule has stable nanometer grain size, stable dispersing coefficient and surface potential suitable for transfect cells. The extracting capability of bone marrow derived dendritic cells (BMDC) for the OVA/PLA/PS microcapsule can be effectively improved. The expression of BMDC surface molecules MHCI, MHCII, CD83 and CD86 is obviously improved, and the secretion of a BMDC polarizing factor IL-12p70 is increased.

The invention relates to a recombinant adenovirus high-expression vector for promoting effective presentation of Hantavirus fusion protein G2S0.7. A G2S0.7-pCAG transfer vector containing G2S0.7 mosaic gene of Hantavirus 76-118 strain and a CAG promoter/enhancer is mainly transformed to promote the effective presentation of the high-expression Hantavirus fusion protein Hantavirus in an organism. By a gene recombination technology, the recombinant adenovirus transfer vector G2S0.7-pCAG containing the mosaic gene G2S0.7 is transformed, and Ub gene is connected to the vector. The recombinant transfer vector and the adenovirus vector are respectively subjected to double digestion, and a recombinant fragment Ub-G2S0.7-pCAG is integrated into the DNA of the adenovirus vector; and after packaging, purification and titer detection, rAd-Ub-G2S0.7-pCAG and recombinant adenovirus rAd-G2S0.7-pCAG containing the G2S0.7 mosaic gene and the CAG promoter/enhancer, which is constructed early by the inventor are respectively used for immunizing C57BL/6 mice, immunological characteristics are researched, and results show that compared with the conventional recombinant adenovirus, the recombinant adenovirus can effectively improve partial humoral immune response level and partial cellular immune response level of organisms of experimental animals.

An anti-disease preparation for animals is prepared through cloning the interleukin-4 and -6 gene of pig, fusing them, configuring the eukaryon expression plasmid VPIl 46 of fusion gene, preparing the chitosan nanoparticles by ion exchange method, dissolving the plasmid in the solution of tripolyphosphate and the deacetyl chitosan solution in acetic acid, and proportional mixing. It can be used to immunize the experimental animal by oral application.

The invention discloses a novel retinoic acid nanoemulsion adjuvant for efficiently enhancing humoral immune response and mucosal immune response and a preparation method and application of the adjuvant. The retinoic acid nanoemulsion adjuvant is prepared from retinoic acid, an oil phase, a surfactant, a cosurfactant and a water phase, wherein the retinoic acid is wrapped by a formed oil-in-water type nanoemulsion adjuvant system. The retinoic acid nanoemulsion adjuvant can be delivered together with multiple antigens (a model antigen OVA, a staphylococcus aureus recombinant protein antigen and a neocoronavirus recombinant protein antigen) respectively, the immune response level of a vaccine antigen can be enhanced after injection immunization, the adjuvant has a good immune protection effect, can induce humoral immune response of a system and efficiently activate immune response of mucosal parts (intestinal mucosa, vaginal mucosa, lung mucosa, gastric mucosa and nasal mucosa) and has great market application value and broad application prospect.

The invention discloses the preparation and application of porcine interleukin-2 gene anti-infectious preparation, which comprises, preparing plasmid chitosan nano particles with ion cross-linking method, dissolving porcine Interleukin-2 gene eucaryon plasmid (VPIL2) into tripolyphosphate solution and chitosan acetic acid solution with a molecular weight of 150000 and deacetylated degree of over 95%, water-bathing at 50 deg. C, keeping constant temperature for 20 minutes, homogeneously mixing plasmid solution and chitosan solution for 5 minutes by right proportion, obtaining VPIL2 plasmid chitosan nano particle liquid with a grain diameter of 40-60mn, Zeta electric potential of +25.6. The preparation has no cell toxicity and can resist the degradation to DNA by nuclease. The DNA nano granular formulation can be used for immunizing experimental animals separately through intramuscular injection or in combination with vaccines.

An anti-infectious nano-class molecular preparation for animals is prepared through fusing the interleukin-4 gene of pig with the interleukin-6 gene of pig, configuring its eukaryon expression plasmid VPIL 46, preparing chitosan nanoparticles by ion exchange method, dissolving said plasmid in the solution of tripolyphosphate and deacetyl chitosan solution in acetate acid, and proportional mixing. It can be used to immunize the experimental animal by intramuscular injection.

The invention relates to a ubiquitin Ub-containing recombinant adenovirus high expression vector for promoting effective presentation of a hantavirus fusion protein G1S0.7. Primarily, G1S0.7 mosaic genes of hantavirus-containing 76-118 strains and a G1S0.7-pCAG transfer vector of a CAG (Cytotoxin Associated Gene) promoter/enhancer are constructed for promoting effective presentation of highly expressed hantavirus fusion protein G1S0.7 by an organism. Compared with the recombinant adenovirus, partial humoral immunity response level and partial cell immunity response level of an animal organism can be effectively improved.