Preparation and application of CpG DNA molecule anti-infection and immunity prepn
An immune preparation, anti-infection technology, applied in genetic material components, sugar derivatives, gene therapy, etc., can solve problems such as low efficiency
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[0058] 1. Design and synthesis of CpG oligonucleotide sequence and its primers
[0059] CpG sequence design and synthesis:
[0060] CpG sequence (see sequence table on page 11 of the manual)
[0061] The sequence is 88 bases in length, contains 11 CpG motifs, and is characterized by containing 3 5’- AACGTT- 3’, 3 5’- GTCGTC- 3’, 1 5’- GACGTT- 3’, 1 5’- ATCGAT- 3’, 1 5’- GTCGTT- 3’, 1 5’- GGCGTT- 3’, 1 5’- GACGTC- 3'feature sequence.
[0062] CpG sequence primer design and synthesis:
[0063] 5’ end: CGAGATCTAACGTTGTC
[0064] 3’ end: CGAGATCTAACGTTAAC
[0065] 2. Preparation of CpG Oligonucleotides for Immunization
[0066] Using the synthesized CpG sequence as a template, it was amplified by PCR (polymerase chain reaction), and the amplified product was dissolved in sterile saline, and its concentration was measured with an ultraviolet spectrophotometer.
[0067] 3. CpG oligonucleotide stimulates the proliferation of animal blood immune cells in vitro
[0068] Under asepti...
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