The invention relates to an
antigen and
adjuvant co-delivery nanometer vaccine applied to
liver cancer immunization therapy.
Sodium alginate and polymine are used as carrier materials,
liver cancer specific polypeptide
antigen phosphatidylinositol proteoglycan 3127-136 peptides(GPC3127-136, AMFKNNYPSL) are used as immunizing antigens,
CpG oligodeoxynucleotide is used as an
adjuvant, and through
static electricity interaction, the
antigen and
adjuvant co-delivery nanometer vaccine is prepared, wherein the
mass ratio of the carrier materials to the immunizing antigens to the adjuvant is 1: (1-10): (0.3-0.8). The nanometer vaccine disclosed by the invention can increase the
endocytosis quantity of dendritic cells (
dendritic cell, DC) to the antigen and the adjuvant, and through raising costimulatory molecules on the surfaces of the DCs and promoting the
secretion of
cytokine of TNF-a, IL-6 and the like, arousing organisms to produce effective immune response is facilitated; and besides, the raw materials are cheap and easy to obtain, the preparation method is easy and easily repeated, large-scale
processing and producing are easy, the
liver cancer immunization therapy effects can be strengthened, and the antigen and adjuvant co-delivery nanometer vaccine has favorable application prospects.