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Preparation of anti-infection CpG oligonucleotide DNA preparation and its application technology

An oligonucleotide and anti-infection technology, which is applied in the direction of anti-infective drugs, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve problems such as low efficiency

Inactive Publication Date: 2006-01-18
SICHUAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since intramuscular injection mainly induces immune response through Th1 cell activation, the efficiency is low, and a large dose of DNA needs to be injected, while a larger amount of DNA is required through the digestive tract for oral administration

Method used

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  • Preparation of anti-infection CpG oligonucleotide DNA preparation and its application technology
  • Preparation of anti-infection CpG oligonucleotide DNA preparation and its application technology
  • Preparation of anti-infection CpG oligonucleotide DNA preparation and its application technology

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0053] 1. Design and synthesis of CpG oligonucleotide sequence and its primers

[0054] CpG sequence design and synthesis:

[0055] CpG sequence (see the sequence list on page 12 of the instruction manual)

[0056] The sequence contains 11 CpG motifs, which are characterized by two 5'- AACGTT- 3', 2 x 5'- GTCGTC- 3', 2 x 5'- GACGTT- 3', 2 x 5'- ATCGAT- 3’, 1 5’- GTCGTT- 3’, 1 5’- GGCGTT- 3’, 1 5’- GACGTC- 3' signature sequence.

[0057] Cp sequence primer design and synthesis:

[0058] 5' end: CGCTGCAGAACGTTGTC

[0059] 3' end: CGCTGCAGAACGACATCG

[0060] 2. Preparation of CpG oligonucleotides for immunization

[0061] Using the synthesized CpG sequence as a template, it is amplified by PCR (polymerase chain reaction), and the amplified product is dissolved in sterilized saline, and its concentration is measured by an ultraviolet spectrophotometer.

[0062] 3. CpG oligonucleotides stimulate animal blood immune cell proliferation in vitro

[0063] Peripheral ...

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Abstract

The invention provides a fragment of CpG oligodeoxynucleotides sequence capable of stimulating proliferation activities of animal immunologic cells, and a method for packaging this fragment of CpG oligodeoxynucleotides to prepare anti-infection DNA nano granular formulation, the use of the CpG nano particles as novel anti-infection molecular immune enhancement agent is also disclosed. The method comprises packaging CpG oligodeoxynucleotides with chitosan nano particles, immunizing experimental animals with the prepared DNA nano granular formulation separately or synergically through intramuscular injection or oral administration, finally detecting the cell and body fluid immunity indexes of the experimental animal.

Description

technical field [0001] 1. Biotechnology 2. Molecular Immunology Background technique [0002] In the 21st century, human health problems have become increasingly prominent. While antibiotics and other chemical drugs are used to effectively treat infectious diseases, many drug-induced diseases and bacterial resistance to antibiotics have also emerged. At present, due to the excessive reliance on antibiotics to control infectious diseases, the problem of bacterial resistance has resulted. In the past decade or so, the multi-drug resistance and all-drug resistance of bacteria to antibiotics has spread and intensified, making many infectious diseases difficult to control. For example, infectious diseases caused by Mycobacterium tuberculosis and Escherichia coli 0157 have made a comeback, seriously threatening human life and health. At present, there is an urgent need to develop new anti-infection technology and drugs, effectively enhance the body's immune and anti-infection abi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7088A61K47/36A61K9/14A61P31/00
Inventor 武梅高荣吕学斌王泽洲李江凌李惠吴凯源付满良杨毅
Owner SICHUAN UNIV
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