Preparation and application of pig interleukin-2 gene anti-infectious preparation
An interleukin, anti-infection technology, applied in gene therapy, anti-tumor drugs, introduction of foreign genetic material using vectors, etc., can solve the problems of short retention time, high cost, toxic and side effects, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0070] 1. Cloning of Tibetan pig IL2 gene (TPIL-2 gene)
[0071] Collect 10 mL of blood from healthy Tibetan pig ear vein aseptically, separate Tibetan pig peripheral blood lymphocytes, add 10ug / ml Con A to stimulate, in CO 2 Incubate in an incubator at 37°C for 72 hours, extract total RNA from Tibetan pigs in time intervals (24, 48, 70 hours), and design a pair of Tibetan pig IL-2 gene cDNA amplification primers. The primer sequence is as follows:
[0072] Primer 1: CG GGATCC CAG TAA CCT CAA CTC CTG CCA CAAT,
[0073]Primer 2: GC CAA TTC CAA GTC AGT GTT GAG TAG ATG CTTT.
[0074] Using total RNA as template, add 10×RT-PCR reaction buffer 5μl, 25mM MgCl to the 50μl reaction system 2 10μl, 10mM dNTP5μl, RNase Inhibitor (40units / μl) 1μl, AMV RNase XL (5units / uL) 1μl, AMV-Optimized Taq (5units / uL) 1μl, 5'and 3'primers each 20uM, total RNA 1~2μl, water supplement To 50μl. The RT-PCR cycle parameters are: 50°C, 30min; 94°C, 2min, then 30 PCR cycles (94°C denaturation for 30s, 60°C an...
PUM
Property | Measurement | Unit |
---|---|---|
The average particle size | aaaaa | aaaaa |
Electric potential | aaaaa | aaaaa |
Molecular weight | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com