43 results about "Tibetan pig" patented technology

The invention discloses a method for cultivating a high-quality specialized strain through Tibetan pigs. Duroc and the Tibetan sows are utilized for hybridization to breed a grading first-filial generation, Duroc boars in a core group and breeding core group sows of the grading first-filial generation are used for hybridization to serve as a grading second-filial generation, the Duro boars in thecore group and the breeding core group sows of the grading second-filial generation are used for hybridization to serve as a grading third-filial generation, the Duro boars in the core group and the breeding core group sows of the grading third-filial generation are used for hybridization to serve as a grading fourth-filial generation, grading fourth-filial generation boars and sows are subjectedto athwart crossing to serve as a zero generation, and athwart crossing is fixed; then generation breeding is carried out, and the high-quality specialized strain is obtained through breeding. The bred specialized strain has the straight back and belly, strong four limbs and the solid limbs and hoofs, achieves good body combination, and is developed in leg and buttock, good in fecundity, good in fattening performance, high in feed conversion rate, high in carcass performance, high in meat quality and free of PSE or DFD meat, the longissimus dorsi pH ranges from 6.0 to 6.5, and the rear leg proportion is larger than 35%.

The invention relates to a new bacillus, which is classified and designated as Bacillus pumilus BY-1. The bacillus pumilus is separated from fresh excrement of a Tibetan pig; the bacillus pumilus is capable of generating cellulase and has the ability of degrading sodium carboxymethylcellulose; after 96 h of culture at 37 DEG C and 220 rpm, the activity of the carboxymethylcellulase is 0.92 mu mol/ml.min. With the gene sequence of the endonuclease gene bglC of Bacillus pumilus SAFR-032 as reference, a designed primer is successful in amplifying the endo-beta-1,4-glucanase gene of the Bacillus pumilus BY-1, which is designated as bglC-BY; and through NCBI blast (National Center for Biotechnology Information, Basic Local Alignment Search Tool), the similarity of the gene to the bglC is 88%, and the similarity thereof to the gene of Bacillus pumilus endoglucanase A precursor(EglA) is higher, up to 96%; therefore, the expression of pET expression system is realized and induction is started when the absorbance A595 of the bacterial fluid is 0.7534 at a culture temperature of 30 DEG C and 220 rpm; the activity is highest in 42 hours after the induction; and the highest activity of the extracellular enzyme is measured to be 0.5434 mu mol/ml.min.

The invention discloses a method for breeding a high-quality pig complete set line by aid of Tibetan pigs. The method includes steps: (1) the Tibetan pigs and Meishan pigs serve as breeding materials to breed a synthetic line, namely a Tibetan-Meishan synthetic line, and the Tibetan-Meishan synthetic line serves as female parents of the complete set line; (2) Berkshire pigs serve as the first male parents of the complete set line, the Tibetan-Meishan synthetic line serves as the female parents, and sows of the parents' generation of the complete set line are obtained after hybridization; (3) durocs serve as terminal male parents to be hybridized with the sows of the parents' generation to obtain high-quality commercial pigs of the complete set line. Pigs in the complete set line bred in the method are identical in body forms and appearances, hair on the whole pig bodies is black, and brown pigs and white pigs are few; heads of the pigs are light, mouths of the pigs are straight, and ears of the pigs are median in size; backs and bellies of the pigs are flat and straight, body and limb combination is good, and legs and buttocks are well developed. The meat quality is good, production efficiency is high, weight of the pigs can reach 90 kg at 187.22 days of age, the feed conversion rate is 3.14, the lean meat percentage is 57.72%, and the intramscular fat content is 4.07%.

The invention discloses a method for ecological raising of fruit-bearing forest Tibetan pigs. The method comprises the following steps: (1) selecting a fruit-bearing forest land, and enclosing the fruit-bearing forest land to form a natural pig farm; (2) disinfecting the pig farm; (3) placing suckling pigs of the Tibetan pigs, which are ablactated for 10-15 days, into the farm to start ecological raising, and from placing to the end of the first month, feeding the pigs with a special feed for 3-4 times every day and 250-350g for each suckling pig every time, and feeding the pigs with a cooked pig feed 1-2 times every day and 500-600g for each suckling pig every time; (4) in the period between the second month and the fourth month from the start of the ecological raising, feeding the pigs with the special feed for 4-5 times every day and 1-1.5kg for each pig every time, and feeding the pigs with the cooked pig feed for 1-2 times every day and 2-3kg for each pig every time; and (5) after the fourth month, feeding the pigs with the special feed for 4-5 times every day and 2-3kg for each pig every time, and feeding the pigs with the cooked pig feed for 1-2 times every day and 4-6kg for each suckling pig every time. The Tibetan pigs raised by the method disclosed by the invention have the characteristics of good pork quality, strong immunity, high nutritive value and good mouthfeel.

The invention discloses a Tibetan pig source bacillus and application thereof. Via bacteria morphology identification, biochemistry identification and molecular identification, the disclosed Tibetan pig source bacillus is named as Bacillus subtilis BY-2 according to classification, and the preservation number of the Tibetan pig source bacillus is CCTCC NO: M2012535. The Bacillus subtilis BY-2 is separated from contents accommodated in the caecum of a Tibetan pig, can generate a higher cellulose clastic enzyme, and has the ability to degrade sodium carboxymethyl cellulose.

An anti-infectious immunopotentiator for animals is prepared through cloning the interleukin-4 gene of Tibetan pig, configuring its eukaryon expression plasmid VTPIL-4, preparing chitosan nanoparticles from deacetyl chitosan, and using said chitosan nanoparticles for molecular packaging of plasmid VTPIL-4. It can be used for immunizing experimental animal by intramuscular injection.

The invention relates to the field of animal breeding, and particularly discloses a new Tibetan pig strain cultivation method. A specific Tibetan pig selection indicator is formulated according to the characteristics of the Tibetan pig variety, and two selection index calculation equations are formulated according to the breeding target. Tibetan pigs with different consanguinities are taken as the basic group zero generation, and the first-generation pig group is selected and reserved according to the 25 kg weight day age breeding value, the backfat thickness breeding value and the litter size breeding value. Selection is conducted in two directions starting from the second-generation pig group, continuous breeding of three to four generations is conducted according to the formulated paternal line selection index and maternal line selection index, and then the new Tibetan pig strain I (fast growth strain) and II (high prolificacy strain) are obtained. High-quality and high-yield new Tibetan pig strain commercial pigs can be produced with the strain II serving as the female parent and the strain I serving as the male parent. New strain pigs have the advantages of being high in growth speed, large in litter size, high in meat quality, high in cold resistance and stress resistance and the like.

The invention discloses a method of identifying a Tibetan pig and meat products based on chemometric analysis, which includes steps: a pork sample is preprocessed; volatile flavor substances in the pork sample are extracted, volatile flavor substances are subjected to qualitative and quantitative analysis by adopting a gas-chromatography-smelling-mass spectrometry combined instrument, and the flavor information is obtained; the flavor information is analyzed by adopting a partial least squares discriminant analysis method, and a Tibetan pig discriminant model is built; and the flavor information of an unknown pork sample is obtained and inputted to the discriminant model, and whether the unknown pork sample is a Tibetan pig is identified according to the output result. The method has the advantages of convenient, quick and accurate identification.

The invention discloses an ecological breeding method of Tibetan pigs. The method comprises the following steps of 1 leading to Enshi, wherein in a cool weather, the Tibetan pigs are led to the place with the elevation of 2,000 meters from the place with the elevation of 3,000 meters through a transportation vehicle to be fed for 15 days to 18 days, then led to the place with the elevation of 1,000 meters or below to be fed for 15 days to 18 days and finally led to the place with the elevation ranging from 500 meters to 600 meters to be bred outside cages; 2 feeding, the Tibetan pigs are fed on 80% of selenium-enriched green feed and 20% of concentrated feed all the year round in the stocking place and fed once in the forenoon and afternoon every day, and medicated feed is forbidden in the whole process; 3 farrowing. According to the ecological breeding method of the Tibetan pigs, the maximum survival rate can reach 98% or above.

The invention provides a special Chinese herbal feed additive for Tibetan pigs, and relates to the technical field of feed. The special Chinese herbal feed additive consists of the following ingredients in parts by mass: 2 to 4 parts of dark plums, 3 to 5 parts of escoparone, 2 to 4 parts of astragalus hoangtchy, 5 to 8 parts of codonopsis pilosula, 3 to 5 parts of acanthopanax senticosus, 4 to 6 parts of malts, 1 to 3 parts of sodium bicarbonate, 2 to 4 parts of black tea, 3 to 5 parts of felwort, 3 to 5 parts of tuckahoe, 2 to 4 parts of radices gentianae macrophyllae and 3 to 5 parts of angelica. The special Chinese herbal feed additive for the Tibetan pigs has the advantages that the contents of various functional nutritional ingredients in pork can be improved; the quality and the flavor of artificially fed pork type products can be improved; the meat quality grade is improved; heavy metal ions in muscles of pig bodies can be reduced; and the generation of germs in the intestinal tracts of the pigs and the quantity of bacillus coli can be reduced.

The invention relates to the technical field of animal husbandry, and provides a method for ecological breeding of Tibetan pigs. The method includes the steps that mountainous grassland with lush green grass is selected to serve as a playground type grazing region, perennial herbaceous plants are mixedly sown and planted on the grassland, and then various kinds of forage are mixedly sown every year after; an ecological pig house is built, silage forage and the like are manufactured, during breeding, one or more of oat, cyperus esculentus, grassiness, chicories, alfalfa, the silage forage and natural weeds are supplemented to the pig house irregularly, and after the pigs freely eat, the pigs are driven into a playground for short-time grazing. By planting various kinds of forage in the playground, more than 100 commercial pigs can be bred on each mu of playground grassland, and large-scale ecological breeding is achieved. According to the method, a modern bioengineering breeding mode isused for disease control, and natural raw materials are used for supplementing microelements to improve the immunity and disease resistance of the pigs; protein raw materials and vitamins are provided for the pigs through manually planted forage, so that large-scale ecological breeding of the Tibetan pigs is achieved.

The invention discloses preparation and application of a recombinant porcine interleukin-23 (IL-23) enhanced porcine circovirus 2 (PCV2) vaccine immunoadjuvant. The P40 and P19 gene fragments of a Tibetan pig IL-23 gene is obtained by using a gene cloning technology for the first time, and a 2A self-shearing expression technology is used for linking the P40 and P19 genes, so that a complete IL-23gene sequence is obtained; then, an In-Fusion cloning method is used for enabling the complete IL-23 gene to be constructed into an eukaryotic secreting type expression vector VR1020; chitosan nanoparticles are prepared by wrapping IL-23 plasmid with chitosan, and experimental animals are inoculated with the chitosan nanoparticles and a PCV2 vaccine at the same time; when pigs are used as the experimental animals, the results indicate that the IL-23 eukaryotic transfection plasmid can effectively enhance the immune response of the pigs to the PCV2 vaccine and promote the growth of vaccinated animals; a new technology is provided for the development of the new and efficient PCV2 immunoadjuvant.

The invention discloses a black pig breeding method. The black pig breeding method comprises the following steps that firstly, a primary group is selected, a purebred Meishan pig is selected as a female parent and a purebred Tibetan pig is selected as a male parent, hybridizing is carried out, and after the Meishan pig female parent farrowing, a sow piglet is selected as a first-generation female parent for later use; secondly, a second-generation group is selected, the first-generation female parent piglets in the first step are bred to an estrus period, purebred Wujin pigs are selected as male parents, hybridizing is carried out, and sow piglets are selected as second-generation female parents for later use after the first-generation female parents give birth; thirdly, breeding pigs are selected, purebred Duroc pigs are selected as male parents for hybridization when the second-generation female parent piglets in the second step are fed to the estrus period, and the piglets are taken as the breeding pigs after the second-generation female parents give birth; and fourthly, the breeding pigs are bred, and in the breeding process, the breeding pigs are fed with feed three times. The piglets bred through the breeding method are high in growth speed and high in disease resistance, meanwhile, the feed is used for feeding the piglets, growth of the piglets can be promoted, and the feeding amount and the disease resistance of the piglets are improved.

The invention relates to a hybrid production method of high-quality pigs, and belongs to the field of genetic breeding of animals. Duroc pigs are adopted as male parents, Guangdong small-ear spotted pigs are adopted as female parents, then the first-filial generations which are Duroc-spotted binary hybrid pigs are adopted as female parents, Tibetan pigs are adopted as male parents, and hybridization is performed so as to obtain the high-quality pigs finally. The high-quality pigs inherits a high growth rate and a high feed utilization rate of the American Duroc pigs, adaption to high-temperature and moist environments, high fertility, good suckling performance of sows and a long service life of the Guangdong small-ear spotted pigs, good pork quality of the Tibetan pigs and other excellentqualities. It is shown through tests on reproductive performance, growth performance, slaughter performance and pork quality performance that the high-quality pigs have the following advantages of high reproductive power, large size, fast growth and development, a high feed utilization rate, a high rate of lean pork, good pork quality, fresh and tender pork, unique flavor and suitability for popularization and feeding.

The invention discloses a Tibetan rose sausage and a preparation method thereof. The Tibetan rose sausage comprises the following raw materials in parts by weight: 96-108 parts of fresh Tibetan pork,4-6 parts of white sugar, 0.4-0.7 parts of salt, 0.04-0.06 parts of white pepper, 1.6-2.5 parts of fermented glutinour rice, 0.004-0.018 parts of chicken essence, 0.005-0.016 parts of MSG, 0.001-0.003parts of sodium D-isoascorbate, 2.5-3.6 parts of white wine, and 4-6.4 parts of Tibetan pig blood; wherein the weight ratio of fat to lean meat in fresh Tibetan pork is 3:1. The method has wide sources of raw materials and simple preparation method, and is suitable for large-scale industrial production. The finished product prepared by the invention has good taste and chewiness, and can meet people's edible requirements.

The invention discloses a cultivation method for improving reproductive performances of Diqing Tibetan pigs. The method comprises the following steps: mating Diqing boars and Diqing Tibetan sows, andscreening wild Tibetan boars; mating Duroc boars and Saba sows, and screening Dusa sows; feeding the screened wild Tibetan boars to an age of 5-6 months, feeding the screened Dusa sows to an age of 6-7 months so as to use after oestrum once; performing first mating on the wild Tibetan boars within 12 hours after the second oestrum of the Dusa sows, and compounding every 12 hours; performing supplementary feeding on piglets at an age of 7-10 days, and weaning the piglets at an age of 53-60 days. According to the method disclosed by the invention, a utilization manner of the Diqing Tibetan pigsis innovated as follows: the boars are respectively hybridized with the Diqing Tibetan pigs, the Duroc pigs and the Saba sows, and hybridization is performed between two hybrids. The hybrid pig breedsare excellent in reproductive performances and achieve hybrid vigor in aspects such as growth, carcass and meat quality, and the effect is obvious.

The invention relates to the technical field of Tibetan pig breeding and particularly relates to a breeding method for increasing farrowing rate of Tibetan pigs. The method comprises the following measures: site construction, growing period breeding, ovulation regulator adding and embryonic development accelerant adding, wherein an ovulation regulator is prepared from Chinese chive seeds, herba epimedii, semen cuscutae and N-carbamylglutamic acid, and an embryonic development accelerant is prepared from radix codonopsis pilosulae, atractylodes macrocephala, radix paeoniae alba, radix scutellariae, VE and melatonin. According to the method, through adding the plant accelerant at two vital links, i.e., estrus ovulation and pregnant farrowing of the Tibetan pigs, the reproductivity of sows isimproved, the litter size is stable, piglets easily survive, the estrus rate of Tibetan sows of an experimental group is increased by 20% to 24% compared with that of a control group, the average litter size of the experimental group is increased by 1.6 to 1.9 compared with that of the control group, and the economic benefit is remarkable.

The invention belongs to the technical field of biology, and particularly relates to application of protein, and the protein is at least one of the following proteins: a talin1 protein with a sequence shown as an amino acid sequence NO.1; a Profilin protein, wherein the sequence of the Profilin protein is shown as an amino acid sequence NO.2; an HGF activator protein, wherein the sequence of the HGF activator protein is shown as an amino acid sequence NO.3; and a Carbonic anhydrase protein, wherein the sequence of the Carbonic anhydrase protein is shown as an amino acid sequence NO.4. The protein is used as a marker for Tibetan pig early pregnancy identification. Another purpose of the invention is to provide a protein marker composition for Tibetan pig early pregnancy identification.

The invention discloses Diqing Tibetan pig fodder containing walnut cakes and a preparation method of the Diqing Tibetan pig fodder. The fodder is prepared from, by weight, 50.0-65.9 kg of corn, 9.0-23.0 kg of bean pulp, 2.0-9.0 kg of wheat bran, 2.0-22.9 kg of mixtures of rice chaff and husks, 0.5-8.0 kg of walnut cakes, 3.0 kg of fish meal and 4.0 kg of premixed materials. In order to solve theproblems that due to insufficient and unbalanced nutrients of existing Diqing Tibetan pig fodder, and especially insufficient protein, Diqing Tibetan pigs grow slowly, and local protein feed resourcesare quite scarce, on the basis of nutrition demand of the Diqing Tibetan pigs, the walnut cakes are utilized as protein feed raw materials, a series of formulas are provided to meet the nutrition demand of the Diqing Tibetan pigs in different physiology stages, and the growth speed of the Diqing Tibetan pigs is increased, and the fodder conversion efficiency is improved.

The invention belongs to the technical field of microbial fermentation and livestock breeding, and discloses a microecological preparation and application thereof in breeding of Tibetan pigs. The viable count ratio of bifidobacterium to lactobacillus to streptococcus faecalis in the microecological preparation is (1.5 to 2.5): (1 to 2): (0.05 to 0.15). The main fermentation environments in the intestinal tracts of the Tibetan pigs are simulated, green feed is adopted for fermentation, and a scientific feed formula is developed according to the variety and day age of the pigs, so that a set ofeconomic feeding method is invented. When the fermented feed is adopted to breed the Tibetan pigs, the growth of the Tibetan pigs can be promoted, the meat quality can be improved, high-quality pork can be produced, the breeding and production costs can be reduced, the breeding range of the Tibetan pigs is expanded advantageously, and the economic value of the Tibetan pigs can be improved.