43 results about "Tibetan pig" patented technology

The invention discloses tibetan-pig IL-12 and an application of recombinant plasmid thereof in preparing a PCV2 vaccine immunoadjuvant. The invention provides a protein which consists of an IL-12 protein P40 subunit, 2A, TPA and an IL-12 protein P35 subunit, wherein the amino acid sequence of 2A is the 324-344 site of a sequence 2, and the amino acid sequence of the TPA is the 345-369 site of the sequence 2. In the experiment, chitosan is used for wrapping IL-12 plasmid to prepare chitosan nano particles, and the chitosan nano particles and PCV vaccines are inoculated into experimental animals simultaneously, a mouse and a pig are respectively adopted as the experimental animals, and results prove that the IL-12 eukaryotic transfection plasmid can enhance immune response of the PCV vaccines effectively to provide an idea for developing a novel high-efficiency PVC immunoadjuvant.

The invention discloses an ecological breeding method of Tibetan pigs. The method comprises the following steps of 1 leading to Enshi, wherein in a cool weather, the Tibetan pigs are led to the place with the elevation of 2,000 meters from the place with the elevation of 3,000 meters through a transportation vehicle to be fed for 15 days to 18 days, then led to the place with the elevation of 1,000 meters or below to be fed for 15 days to 18 days and finally led to the place with the elevation ranging from 500 meters to 600 meters to be bred outside cages; 2 feeding, the Tibetan pigs are fed on 80% of selenium-enriched green feed and 20% of concentrated feed all the year round in the stocking place and fed once in the forenoon and afternoon every day, and medicated feed is forbidden in the whole process; 3 farrowing. According to the ecological breeding method of the Tibetan pigs, the maximum survival rate can reach 98% or above.

The invention discloses a method for breeding a high-quality pig complete set line by aid of Tibetan pigs. The method includes steps: (1) the Tibetan pigs and Meishan pigs serve as breeding materials to breed a synthetic line, namely a Tibetan-Meishan synthetic line, and the Tibetan-Meishan synthetic line serves as female parents of the complete set line; (2) Berkshire pigs serve as the first male parents of the complete set line, the Tibetan-Meishan synthetic line serves as the female parents, and sows of the parents' generation of the complete set line are obtained after hybridization; (3) durocs serve as terminal male parents to be hybridized with the sows of the parents' generation to obtain high-quality commercial pigs of the complete set line. Pigs in the complete set line bred in the method are identical in body forms and appearances, hair on the whole pig bodies is black, and brown pigs and white pigs are few; heads of the pigs are light, mouths of the pigs are straight, and ears of the pigs are median in size; backs and bellies of the pigs are flat and straight, body and limb combination is good, and legs and buttocks are well developed. The meat quality is good, production efficiency is high, weight of the pigs can reach 90 kg at 187.22 days of age, the feed conversion rate is 3.14, the lean meat percentage is 57.72%, and the intramscular fat content is 4.07%.

The invention relates to a new bacillus, which is classified and designated as Bacillus pumilus BY-1. The bacillus pumilus is separated from fresh excrement of a Tibetan pig; the bacillus pumilus is capable of generating cellulase and has the ability of degrading sodium carboxymethylcellulose; after 96 h of culture at 37 DEG C and 220 rpm, the activity of the carboxymethylcellulase is 0.92 mu mol / ml.min. With the gene sequence of the endonuclease gene bglC of Bacillus pumilus SAFR-032 as reference, a designed primer is successful in amplifying the endo-beta-1,4-glucanase gene of the Bacillus pumilus BY-1, which is designated as bglC-BY; and through NCBI blast (National Center for Biotechnology Information, Basic Local Alignment Search Tool), the similarity of the gene to the bglC is 88%, and the similarity thereof to the gene of Bacillus pumilus endoglucanase A precursor(EglA) is higher, up to 96%; therefore, the expression of pET expression system is realized and induction is startedwhen the absorbance A595 of the bacterial fluid is 0.7534 at a culture temperature of 30 DEG C and 220 rpm; the activity is highest in 42 hours after the induction; and the highest activity of the extracellular enzyme is measured to be 0.5434 mu mol / ml.min.