The invention relates to a raman spectrum-based
fast measurement method for a
motile sperm deoxyribonucleic acid (
DNA). The invention adopts the following technical scheme: collecting a
semen sample by a
test tube; naturally liquefying
semen in a room-temperature environment; absorbing the liquefied
semen and adding to a centrifugal tube containing
density gradient centrifugate; absorbing upper seminal
plasma after centrifuging, discarding, then adding a PBS
buffer solution and centrifugally washing again; absorbing supernatant, discarding, adding the PBS
buffer solution to prepare a
sperm suspension, and incubating; attaching a
metal bottom plate to a culture dish; adding the PBS
buffer solution, and absorbing the incubated
sperm suspension, soaking in the culture dish, wherein the
sperm swims outside and contacts the
metal bottom plate; setting a spectrum response range, integrating time and the excitation light
wavelength, so as to obtain raman spectrum data from the head part of the sperm. The
motile sperm swimming out from the pointed-end part of a sucker can be attached to the
metal bottom plate by operation of a thin tube
pipette, and the damage condition of the
sperm DNA is evaluated by measuring the sperm of which the head is attached to the metal bottom plate.