32 results about "Semen sample" patented technology

The present disclosure relates to methods of identifying, differentiating between and / or sorting sperm cells, or more particularly, X-chromosome bearing sperm (X-CBS) and Y-chromosome bearing sperm (Y-CBS) cells, in a sperm sample, the methods comprising determining a level of a Reactive Oxidant Species and / or a Cellular Change mediated thereby (ROSCC) in each of a plurality of sperm cells in the sperm sample; and based on the determined ROSCC levels, identifying, differentiating between and / or sorting X-CBS and Y-CBS cells in at least a portion of the plurality of sperm cells. Also described are enriched or purified X-CBS and Y-CBS cells, and uses thereof.

The present application belongs to the technical field of bull semen quality evaluation, and particularly relates to a local bull semen quality evaluation method and system based on multi-source data analysis. The method comprises: when real-time data corresponding to a current semen sample is acquired, executing a quality evaluation process to generate a comprehensive score of the current semen sample; based on the offset amplitude of sample data generated by the evaluation model in the benchmark test, determining a main parameter and at least one subsidiary parameter from a plurality of to-be-tested parameters associated with the current semen sample. The present application calculates a subsidiary score according to the subsidiary parameter, and adjusts the subsidiary score by using an external parameter to generate a comprehensive score; and a multi-dimensional dynamic evaluation framework is constructed to consider a plurality of to-be-tested parameters of the semen itself, and the external parameter is quantified and integrated into the final score, so that the evaluation result can dynamically adapt to different environments and individual conditions, and overcome the limitation of single evaluation dimension in the prior art.

This utility model discloses an improved semen DNA collection card for assisted reproductive technology, belonging to the field of medical supplies technology. It includes a folding card with an upper cover, a lower cover, and a bottom surface. The bottom surface is located between the upper and lower covers. The lower cover has a sampling filter paper surface, and a PVC diaphragm is provided in the middle of the sampling filter paper surface to divide it into a pre-processing semen collection area and a post-processing semen collection area. This utility model stores the patient's unprocessed semen in the pre-processing semen collection area and the processed semen in the post-processing semen collection area. The PVC diaphragm between the two areas prevents interference and cross-contamination between the two semen samples, facilitating DNA comparison before and after semen processing. The folding card also facilitates transportation and transfer, allowing for the storage of both pre- and post-processing semen within a single card, which is beneficial for clinical research.

The application discloses an information processing method and device for detecting sperm quality, equipment and medium, applied to a computer device, relates to the field of biomedical detection, and comprises the following steps: injecting an original semen sample into a preset microfluidic chip to dilute the original semen sample, and collecting multi-modal diluted sample data; performing parallel feature analysis on the multi-modal diluted sample data to determine corresponding multiple sperm characteristics; performing feature fusion on the multiple sperm characteristics based on a preset feature weight distribution network to obtain sperm fusion characteristics; determining corresponding several sperm quality parameters based on the sperm fusion characteristics, and determining the sperm quality of the original semen sample based on the several sperm quality parameters. Therefore, the detection efficiency and result reliability can be improved through highly automated synchronous multi-modal data acquisition and deep fusion analysis.

The invention is applicable to the field of in-vitro diagnostic equipment, and discloses a sample analyzer. The sample analyzer comprises a sample introduction device, a sample distribution device, a first visible component detection device, a second visible component detection device and a controller, wherein the first visible component detection device comprises a first microscopic imaging device. The controller is configured to at least output a kinetic analysis result of sperms in the semen sample according to an image obtained by executing a first shooting action on the semen sample by the first microscopic imaging device; and at least outputting a morphological analysis result of at least part of the visible components in the first body fluid sample according to detection data obtained by performing the visible component detection action on the first body fluid sample by the second visible component detection device. According to the invention, one sample analyzer can be used for carrying out visible component analysis on a semen sample and another type of body fluid sample, so that the purchase, use and maintenance cost of the sample analyzer for analyzing the semen sample can be reduced.

The invention discloses a method for evaluating damage of ionizing radiation to male sperm quality, which belongs to the technical field of reproductive biology, and comprises the following steps: establishing a dose-effect database model between ionizing radiation dose and male sperm quality based on a logistic regression model, determining a mapping relation between sperm quality rating and fertility recovery average time; collecting a to-be-detected semen sample exposed by ionizing radiation, comparing the to-be-detected semen sample with the dose-effect database model, evaluating the male sperm quality rating, and predicting the fertility recovery time based on the male sperm quality rating and the mapping relation.

This invention discloses a semen-specific mRNA-cSNP primer composition, kit, and method based on SNaPshot technology, belonging to the field of molecular identification technology. The primer composition provided by this invention includes amplification primers with nucleotide sequences as shown in SEQ ID NO. 1-34 and single-base extension primers as shown in SEQ ID NO. 35-52. This invention further provides a method for identifying human semen samples, including extracting sample mRNA, reverse transcription to obtain cDNA, performing an amplification reaction using amplification primers, and then performing a single-base extension reaction using single-base extension primers. Experimental results show that the identification method of this invention has advantages such as high specificity, high sensitivity, and good reproducibility, and can accurately identify individual semen donors, which is of great significance for achieving accurate semen-related criminal investigations and forensic identification.

This invention relates to the field of sperm detection technology, specifically disclosing an automated sperm density detection chamber integrating sample mixing and temperature control functions. The chamber comprises, from bottom to top, a light-transmitting bottom layer, a microchannel layer, and a light-transmitting cover layer. The light-transmitting bottom layer has, from bottom to top, a glass substrate layer, an ITO heating electrode layer, a first light-transmitting insulating layer, a first ITO mixing electrode layer, and a second light-transmitting insulating layer. The light-transmitting cover layer has, from top to bottom, an upper glass surface layer, a second ITO mixing electrode layer, and a third light-transmitting insulating layer. The microchannel layer forms microchannels, and the light-transmitting cover layer has an inlet port and an exhaust port communicating with the microchannels. This invention achieves stable constant temperature control of the semen sample within the microchannels, effectively avoiding the adverse effects of environmental temperature fluctuations on sperm characteristics and detection results. Furthermore, it enables in-situ automatic mixing of the semen sample, improving the accuracy and repeatability of sperm density detection results.

The invention discloses application of pseudomonas as a marker in evaluation of bull semen quality, and belongs to the technical field of animal breeding and microbiomics. The relative abundance of Pseudomonas in a bull rumen fluid with high semen quality is significantly higher than the relative abundance in a bull rumen fluid with low semen quality. By detecting the relative abundance, the bull semen quality can be evaluated, and the reproductive performance can be reliably judged on the premise of not depending on a semen sample. Evaluation can be carried out before semen collection for the first time or in a breeding candidate period, potential low-fecundity individuals are screened out in advance, and the breeding risk is reduced.

The present application is applicable to the field of in-vitro diagnostic devices. Disclosed is a sample analyzer. The sample analyzer comprises: a feeding device, a sample distribution device, a microscopic imaging device and a controller, wherein the controller is configured to: on the basis of an image obtained by means of the microscopic imaging device executing a first photographing action on a semen sample, output at least a motility analysis result of sperm in the semen sample; and on the basis of an image obtained by means of the microscopic imaging device executing a second photographing action on a first body fluid sample, output at least a morphological analysis result of at least some formed elements in the first body fluid sample. A first sample container and a second sample container are two mutually independent sample containers, and a first carrier and a second carrier are two mutually independent carriers or the same carrier. The sample analyzer of the present application can perform formed-element analysis on a semen sample and another body fluid sample, thereby facilitating a reduction in purchase, usage and maintenance costs of sample analyzers that can be used for semen sample analysis.

The invention belongs to the technical field of livestock breeding equipment, and discloses a constant-temperature semen storage box which comprises a box body and a placement frame, a placement cavity and a heat preservation cavity are formed in the box body, and an opening is formed in the side face of the box body; the placing frame is slidably arranged on the top surface of the heat conducting plate, the tail end of the placing frame penetrates through the opening, fixing assemblies are arranged on the placing frame, baffles are arranged at the two adjacent fixing assemblies and the two ends of the placing frame, side plates and compression springs are arranged on the baffles, sealing strips are arranged on the side faces of the side plates, oblique angles are formed between the sealing strips and the edges of the side plates, and the sealing strips and the edges of the side plates are matched with the grooves; an auxiliary sealing group arranged at the opening is in contact with the baffle plate. Through the opening in the side face of the box body, the placing frame with the corresponding length can be pulled out when several semen samples are taken, the other semen samples are still located in the box body, and heat exchange is reduced; the opening is blocked through the baffle and the side plates, the auxiliary sealing set is matched, external air is prevented from entering the box body through the opening, the placing frame can be prevented from moving at will, and air convection can be reduced.

The application provides a portable full-automatic fluorescence spectrum sperm activity detection method and system, and belongs to the technical field of sperm activity detection. The method comprises the following steps: placing a semen sample to be detected into a detection kit to which a sperm activity fluorescence biological dyeing agent is added; the sperm activity fluorescence biological dyeing agent comprises a CM-Dil biological derivative activation dyeing solution; fluorescence spectrum information of the semen sample to be detected is acquired through a far-field optical fluorescence microscope and a microscopic optical information acquisition assembly; the fluorescence spectrum information is subjected to data analysis through a spectrum analysis system, so as to obtain survival rate information, development state information and activity classification information of sperm in the semen sample. The application realizes that a person to be detected can complete sperm activity detection by himself without going to a hospital; and the detection result contains the survival rate information, the development state information and the activity classification information of sperm in the semen sample, so that whether the person to be detected has the ability to reproduce can be accurately determined.

The utility model discloses a storage device for semen detection, which comprises a suitcase, an internal storage box body is placed in the suitcase, a semen storage box body is arranged in the internal storage box body, a base is fixed in the suitcase, a pivot is fixed on the base, cover plates are rotatably arranged on two sides of the pivot, one side of each cover plate extends and is fixed with a fastener, and the fastener is fixed on the base. A clamping groove is formed in the side, away from the base, of the suitcase, and the buckling piece is buckled in the clamping groove. The utility model aims to limit and fix the internal storage box body, has strong sealing property, ensures the accuracy of subsequent detection of semen in the semen storage tube, avoids cross contamination among the semen, improves the tidiness of the suitcase, ensures that the suitcase is safer and more sanitary to use, has strong stability of the internal semen tube, and is more convenient to use. The semen tube is effectively prevented from colliding with the inner wall and shaking, and most impact force generated when the device accidentally falls can be reduced.

A device for filtering out non-functional sperm and separating motile sperm may include a main channel having a flow direction from a first end toward a second end of the main channel. The device may include a buffer inlet in fluid communication with the first end of the main channel. The apparatus may include a collection chamber in fluid communication with the second end of the main channel. The device may include a secondary channel in fluid communication with the collection chamber on a downstream side. The apparatus may include a waste chamber in fluid communication with the secondary channel on a downstream side.

The utility model relates to the field of sperm flaking equipment, in particular to a sperm morphology flaking machine, which comprises a pipetting driving device, a pipettor, a first moving device, an overturning mechanism, a liquid conveying mechanism and a hot air gun, the first moving device is matched to drive the pipettor to move so as to achieve the purpose of transferring a semen sample; the turnover mechanism is used for driving the filter cup to turn over, the filter cup is turned over after sperms are filtered out by the filter cup, the filtered sperms are scoured into the sample cup in cooperation with the infusion mechanism, the sperms are diluted, and flaking is facilitated; the first moving device drives the hot air gun to move, so that an air outlet of the hot air gun is aligned with a liquid inlet in the flaking device, and the hot air gun is started to accelerate flaking; through the mutual cooperation of the devices and the mechanisms, the problems of long sheet making time and relatively tedious process when a sample sheet is made at present are solved.

A sperm sorting device is suitable for sorting a plurality of motile sperm from a semen sample, and includes a base and a first sorter positioned on the base. The base includes a receiving space defined by a bottom wall and a surrounding wall. The first sorter includes a first kit and a first permeable membrane. The first kit has a first sleeve for being placed into the receiving space. The first sleeve has a first inner sleeve surface and a first outer sleeve surface. The first permeable membrane is fixed to the first sleeve. The first permeable membrane and the inner wall surface of the bottom wall and the surrounding wall define a first sample receiving chamber for receiving the semen sample. In this way, the motile sperm can naturally swim upward and pass through the first permeable membrane, and the quality of sperm sorting can be improved.

This invention discloses a barrel-shaped sperm selection device for selecting highly motile sperm from semen samples. The device includes a container with a porous membrane inside, which divides the interior of the container into an initial chamber and a filtration chamber. This allows the semen sample to move through the porous membrane to the filtration chamber, achieving the selection purpose. The advantages of this invention are: the overall barrel shape of the device, with the porous membrane arranged on a hub along the axial direction of the cylindrical container to form inner and outer cavities, simplifies the structure and optimizes the principle, greatly reducing the requirements for molds and processes. The barrel-shaped structure with inner and outer cavities prevents semen overflow even when the semen sample volume is too large; and prevents reduced selection rates due to air bubbles even when the semen sample volume is too small.

The invention comprises a housing with a separation chamber. The separation chamber may comprise a first chamber, a second chamber and a membrane between the first chamber and the section chamber. The membrane prevents non-motile cells from a semen sample in the first chamber, and allows motile cells from the semen sample, to enter the second chamber. The housing has an inlet for the semen sample to enter the separation chamber and at least one outlet opening for extracting the motile cells from the separation chamber. A hollow interior of a straw is connected with the at least one outlet opening of the separation chamber of the housing. A fluid pressure difference extracts motile cells from the separation chamber to the hollow interior of the straw.

The present application relates to the technical field of fertility detection, and discloses a male fertility rapid detection method and a detection system thereof, which comprises the following steps: step 1, washing hands, first clean hands before testing to avoid pollution; step 2, taking out a semen collector, taking out the semen collector from a packaging bag; step 3, unfolding the semen collector, opening and preparing the semen collector to facilitate receiving a semen sample; step 4, collecting the semen sample, holding the handle of the semen collector and injecting the semen into the semen collector; and step 5, opening the seal of a lysis buffer, opening the seal of the bottle mouth of the lysis buffer. The present application can complete the detection within 15 minutes through simple operation steps, greatly improves the convenience of detection, simultaneously adopts a simple reagent kit and an operation mode without professional equipment, greatly reduces the detection cost, and through scientific design and verification, can ensure the accuracy and repeatability of the result.

The invention relates to a microfluidic device, method, and use for the continuous, label-free isolation of motile, morphologically normal spermatozoa from semen samples This invention offers a gentle, reliable, and efficient platform for sperm selection, serving as an alternative to centrifugation-based techniques in ART laboratories. By maintaining the structural and genetic integrity of sperm, it improves fertilization success, embryo quality, and pregnancy outcomes. The platform also reduces variability between technicians and limits exposure to reactive oxygen species (ROS), ensuring consistent clinical performance. Beyond its application in fertility treatments, the system can be used as a diagnostic tool to assess sperm motility disorders, susceptibility to oxidative stress, and DNA fragmentation dynamics under different environmental conditions or drug exposures.

This invention discloses a nanopore sequencing method for detecting full-length 16S rRNA amplicon in human semen samples. The method includes sample pretreatment and DNA extraction; nested PCR amplification using outer primers 16S-27F / 1492R and inner primers 16S-340F / 1390R to obtain target long amplicon lengths of 1.0kb to 1.1kb; construction of a nanopore library and single-molecule long-read sequencing; deep filtering of the raw data with a quality score Q≥20 and read lengths between 1000bp and 1700bp; and finally, feature clustering and taxonomic annotation. This invention addresses the characteristics of low biomass and high host DNA background in human semen samples by employing innovative specific nested primer design and customized data cleaning strategies. This effectively avoids non-specific interference and significantly improves the classification resolution and data fidelity of the semen microbiome at the genus and species levels, providing a precise detection method for analyzing the reproductive microecology of infertile men.

A method for collecting and preserving semen samples using a dual vial kit 100 comprising collecting a semen sample in a first vial 105, transferring a predetermined portion of the sample to a second

The application discloses a sperm quality detection device and a preparation method thereof. The sperm quality detection device comprises a base material, a sperm activity detection module, an SP10 detection module and a PSA detection module. The sperm activity detection module comprises a containing cavity and a convex lens. The containing cavity has a detection plane. The convex lens is configured to be able to slide to a sample adding hole along with a sliding assembly and to magnify the image of the detection plane. The SP10 detection module comprises a P10 colloidal gold test strip. The PSA detection module comprises a PSA colloidal gold test strip. The application provides a detection device integrating sperm acrosome enzyme SP10 protein, prostate specific antigen PSA and special counting for a semen sample. The device is simple to operate and can provide a detection result in a short time. The detection result can be judged by the person to be detected independently, and the device is suitable for primary medical units and on-site rapid detection.

The invention relates to a yak X / Y sperm separation and enrichment method based on pH value regulation and a kit thereof, and belongs to the technical field of animal breeding and sex control. The method comprises the following steps: using an Optidyl diluent as a sperm sorting protection solution, adjusting the pH value of the Optidyl diluent through NaOH or HCl, enriching X sperms in a pH range of 6.2-6.4, and enriching Y sperms in a pH range of 6.8-7.0; the method comprises the following specific steps: diluting yak sperm, centrifugally desizing, re-suspending by using a sorting solution with corresponding pH, standing and incubating for 45-90 minutes at 35-40 DEG C and 45 DEG C, realizing natural layering according to the motility difference of X and Y sperms under different pH conditions, respectively collecting upper or lower sperms, and washing and re-suspending to obtain a sex-controllable sperm sample; the method is easy and convenient to operate, low in cost, small in sperm damage, complete in sperm structure and stable in function after separation, suitable for breeding technologies such as artificial insemination and in-vitro fertilization, capable of meeting the requirement for sexual control sperm in yak production and beneficial for optimizing the population structure and improving the breeding efficiency.

The invention discloses a method and a system for diagnosing and typing male infertility based on sperm RNA (Ribonucleic Acid) characteristics. The core of the invention lies in a biological discovery that most RNA can be systematically degraded and removed in the sperm maturation process, so that the total RNA content of healthy and mature sperms is in negative correlation with the functional soundness of the healthy and mature sperms. The method comprises the following steps: (a) obtaining a semen sample of a subject; (b) separating and purifying sperm cells from the sample; (c) quantifying the purified sperms, and determining the total RNA content of each sperm cell so as to obtain a first value; and (d) comparing the first numerical value with a preset reference value, and if the first numerical value is obviously higher than the reference value, indicating that the subject has low fertility or infertility (such as asthenospermia). According to the invention, an objective and quantifiable molecular biomarker is provided by quantifying residual'molecular package 'in the sperm maturation process, and a new way is provided for accurate diagnosis, typing and treatment of male infertility.

The application discloses a method for evaluating damage of male sperm quality caused by ionizing radiation, and belongs to the technical field of reproductive biology, and comprises the following steps: establishing a dose-effect database model between ionizing radiation dose and male sperm quality based on a logistic regression model, and determining a mapping relationship between sperm quality rating and average time for recovery of fertility; collecting a semen sample to be measured exposed to ionizing radiation, comparing the semen sample to be measured with the dose-effect database model, evaluating male sperm quality rating, and predicting time required for recovery of fertility based on the male sperm quality rating and the mapping relationship.

The application discloses a semen sample quality management system based on federal learning and dynamic timing prediction, wherein a data acquisition module (100) is used for cleaning and processing the received original semen sample detection data, a federal learning cooperation module (200) adopts an improved Paillier homomorphic encryption algorithm to encrypt and transmit local model gradient parameters, a dynamic timing prediction module (300) extracts quality features with timing dependence from the semen sample detection data, and performs online incremental training on a prediction model, a multi-modal data fusion module (400) is used for extracting a genomic feature sequence of the semen sample and analyzing stored environmental parameters of the sample, and a quality evaluation decision module (500) fuses the timing prediction model and the genomic feature sequence.

The invention relates to a microfluidic device, method, and use for the continuous, label-free isolation of motile, morphologically normal spermatozoa from semen samples This invention offers a gentle, reliable, and efficient platform for sperm selection, serving as an alternative to centrifugation-based techniques in ART laboratories. By maintaining the structural and genetic integrity of sperm, it improves fertilization success, embryo quality, and pregnancy outcomes. The platform also reduces variability between technicians and limits exposure to reactive oxygen species (ROS), ensuring consistent clinical performance. Beyond its application in fertility treatments, the system can be used as a diagnostic tool to assess sperm motility disorders, susceptibility to oxidative stress, and DNA fragmentation dynamics under different environmental conditions or drug exposures.