A sperm tail recognition method comprises the following steps: step 1, acquiring an image; step 2, acquiring a difference image; step 3, acquiring a tail image; 4, acquiring a sperm head region image;5, combining the tail image and the sperm head region image; and 6, filtering the bright spot area without the long-tail area within a preset distance to obtain a sperm area image. Through acquisition of the difference image, a bright spot area in the semen sample image is filtered out, so that a non-long-tail area is convenient to filter, and an image of impurities or cells, which are greatly different from the sperm structure, in semen is realized. Filtering of bright spot images without long-tail-shaped areas nearby can be achieved through a distance selection method, and then filtering ofbright spot areas of non-sperm cells is achieved. According to the method, impurities in the seminal fluid are accurately filtered, and researchers can count and research the seminal fluid conveniently.