12 results about "Semen sample" patented technology

The present disclosure relates to methods of identifying, differentiating between and / or sorting sperm cells, or more particularly, X-chromosome bearing sperm (X-CBS) and Y-chromosome bearing sperm (Y-CBS) cells, in a sperm sample, the methods comprising determining a level of a Reactive Oxidant Species and / or a Cellular Change mediated thereby (ROSCC) in each of a plurality of sperm cells in the sperm sample; and based on the determined ROSCC levels, identifying, differentiating between and / or sorting X-CBS and Y-CBS cells in at least a portion of the plurality of sperm cells. Also described are enriched or purified X-CBS and Y-CBS cells, and uses thereof.

The present application belongs to the technical field of bull semen quality evaluation, and particularly relates to a local bull semen quality evaluation method and system based on multi-source data analysis. The method comprises: when real-time data corresponding to a current semen sample is acquired, executing a quality evaluation process to generate a comprehensive score of the current semen sample; based on the offset amplitude of sample data generated by the evaluation model in the benchmark test, determining a main parameter and at least one subsidiary parameter from a plurality of to-be-tested parameters associated with the current semen sample. The present application calculates a subsidiary score according to the subsidiary parameter, and adjusts the subsidiary score by using an external parameter to generate a comprehensive score; and a multi-dimensional dynamic evaluation framework is constructed to consider a plurality of to-be-tested parameters of the semen itself, and the external parameter is quantified and integrated into the final score, so that the evaluation result can dynamically adapt to different environments and individual conditions, and overcome the limitation of single evaluation dimension in the prior art.

This utility model discloses an improved semen DNA collection card for assisted reproductive technology, belonging to the field of medical supplies technology. It includes a folding card with an upper cover, a lower cover, and a bottom surface. The bottom surface is located between the upper and lower covers. The lower cover has a sampling filter paper surface, and a PVC diaphragm is provided in the middle of the sampling filter paper surface to divide it into a pre-processing semen collection area and a post-processing semen collection area. This utility model stores the patient's unprocessed semen in the pre-processing semen collection area and the processed semen in the post-processing semen collection area. The PVC diaphragm between the two areas prevents interference and cross-contamination between the two semen samples, facilitating DNA comparison before and after semen processing. The folding card also facilitates transportation and transfer, allowing for the storage of both pre- and post-processing semen within a single card, which is beneficial for clinical research.

The application discloses an information processing method and device for detecting sperm quality, equipment and medium, applied to a computer device, relates to the field of biomedical detection, and comprises the following steps: injecting an original semen sample into a preset microfluidic chip to dilute the original semen sample, and collecting multi-modal diluted sample data; performing parallel feature analysis on the multi-modal diluted sample data to determine corresponding multiple sperm characteristics; performing feature fusion on the multiple sperm characteristics based on a preset feature weight distribution network to obtain sperm fusion characteristics; determining corresponding several sperm quality parameters based on the sperm fusion characteristics, and determining the sperm quality of the original semen sample based on the several sperm quality parameters. Therefore, the detection efficiency and result reliability can be improved through highly automated synchronous multi-modal data acquisition and deep fusion analysis.

This invention relates to the field of sperm detection technology, specifically disclosing an automated sperm density detection chamber integrating sample mixing and temperature control functions. The chamber comprises, from bottom to top, a light-transmitting bottom layer, a microchannel layer, and a light-transmitting cover layer. The light-transmitting bottom layer has, from bottom to top, a glass substrate layer, an ITO heating electrode layer, a first light-transmitting insulating layer, a first ITO mixing electrode layer, and a second light-transmitting insulating layer. The light-transmitting cover layer has, from top to bottom, an upper glass surface layer, a second ITO mixing electrode layer, and a third light-transmitting insulating layer. The microchannel layer forms microchannels, and the light-transmitting cover layer has an inlet port and an exhaust port communicating with the microchannels. This invention achieves stable constant temperature control of the semen sample within the microchannels, effectively avoiding the adverse effects of environmental temperature fluctuations on sperm characteristics and detection results. Furthermore, it enables in-situ automatic mixing of the semen sample, improving the accuracy and repeatability of sperm density detection results.

The present application is applicable to the field of in-vitro diagnostic devices. Disclosed is a sample analyzer. The sample analyzer comprises: a feeding device, a sample distribution device, a microscopic imaging device and a controller, wherein the controller is configured to: on the basis of an image obtained by means of the microscopic imaging device executing a first photographing action on a semen sample, output at least a motility analysis result of sperm in the semen sample; and on the basis of an image obtained by means of the microscopic imaging device executing a second photographing action on a first body fluid sample, output at least a morphological analysis result of at least some formed elements in the first body fluid sample. A first sample container and a second sample container are two mutually independent sample containers, and a first carrier and a second carrier are two mutually independent carriers or the same carrier. The sample analyzer of the present application can perform formed-element analysis on a semen sample and another body fluid sample, thereby facilitating a reduction in purchase, usage and maintenance costs of sample analyzers that can be used for semen sample analysis.

A sperm sorting device is suitable for sorting a plurality of motile sperm from a semen sample, and includes a base and a first sorter positioned on the base. The base includes a receiving space defined by a bottom wall and a surrounding wall. The first sorter includes a first kit and a first permeable membrane. The first kit has a first sleeve for being placed into the receiving space. The first sleeve has a first inner sleeve surface and a first outer sleeve surface. The first permeable membrane is fixed to the first sleeve. The first permeable membrane and the inner wall surface of the bottom wall and the surrounding wall define a first sample receiving chamber for receiving the semen sample. In this way, the motile sperm can naturally swim upward and pass through the first permeable membrane, and the quality of sperm sorting can be improved.

The present application relates to the technical field of fertility detection, and discloses a male fertility rapid detection method and a detection system thereof, which comprises the following steps: step 1, washing hands, first clean hands before testing to avoid pollution; step 2, taking out a semen collector, taking out the semen collector from a packaging bag; step 3, unfolding the semen collector, opening and preparing the semen collector to facilitate receiving a semen sample; step 4, collecting the semen sample, holding the handle of the semen collector and injecting the semen into the semen collector; and step 5, opening the seal of a lysis buffer, opening the seal of the bottle mouth of the lysis buffer. The present application can complete the detection within 15 minutes through simple operation steps, greatly improves the convenience of detection, simultaneously adopts a simple reagent kit and an operation mode without professional equipment, greatly reduces the detection cost, and through scientific design and verification, can ensure the accuracy and repeatability of the result.

This invention discloses a nanopore sequencing method for detecting full-length 16S rRNA amplicon in human semen samples. The method includes sample pretreatment and DNA extraction; nested PCR amplification using outer primers 16S-27F / 1492R and inner primers 16S-340F / 1390R to obtain target long amplicon lengths of 1.0kb to 1.1kb; construction of a nanopore library and single-molecule long-read sequencing; deep filtering of the raw data with a quality score Q≥20 and read lengths between 1000bp and 1700bp; and finally, feature clustering and taxonomic annotation. This invention addresses the characteristics of low biomass and high host DNA background in human semen samples by employing innovative specific nested primer design and customized data cleaning strategies. This effectively avoids non-specific interference and significantly improves the classification resolution and data fidelity of the semen microbiome at the genus and species levels, providing a precise detection method for analyzing the reproductive microecology of infertile men.

The application discloses a method for evaluating damage of male sperm quality caused by ionizing radiation, and belongs to the technical field of reproductive biology, and comprises the following steps: establishing a dose-effect database model between ionizing radiation dose and male sperm quality based on a logistic regression model, and determining a mapping relationship between sperm quality rating and average time for recovery of fertility; collecting a semen sample to be measured exposed to ionizing radiation, comparing the semen sample to be measured with the dose-effect database model, evaluating male sperm quality rating, and predicting time required for recovery of fertility based on the male sperm quality rating and the mapping relationship.