37 results about "Sperm cell" patented technology

The present disclosure relates to methods of identifying, differentiating between and / or sorting sperm cells, or more particularly, X-chromosome bearing sperm (X-CBS) and Y-chromosome bearing sperm (Y-CBS) cells, in a sperm sample, the methods comprising determining a level of a Reactive Oxidant Species and / or a Cellular Change mediated thereby (ROSCC) in each of a plurality of sperm cells in the sperm sample; and based on the determined ROSCC levels, identifying, differentiating between and / or sorting X-CBS and Y-CBS cells in at least a portion of the plurality of sperm cells. Also described are enriched or purified X-CBS and Y-CBS cells, and uses thereof.

The utility model discloses an efficient separator for human seminal spot mixed spots, which relates to the technical field of separators and comprises a centrifugal tube and a detachable sleeve matched with the centrifugal tube, the caliber of the detachable sleeve is consistent with that of the centrifugal tube, and a sleeve cavity for containing biological material evidences is arranged in the detachable sleeve. A plastic filter plate is arranged in the middle of the sleeve cavity, a filter membrane filter layer is arranged at the bottom of the sleeve cavity, and a bottom drainage hole is formed in the bottom of the detachable sleeve. According to the utility model, a two-stage filtering mode of carrier removal filtering and sperm enrichment filtering is constructed in one sleeve, so that a biological material evidence carrier can be efficiently removed, water-soluble substances of human epithelial cells can be quickly filtered, sperm cells can be efficiently separated from related biological material evidences only by using a small amount of biological material evidences, and the operation is simple and convenient. The test speed and efficiency are greatly improved, difficult biological material evidence treatment is simplified, the experiment repeatability is good, the applicability is high, biological material evidence samples are saved, and the cost of related experiments is reduced.

This invention discloses a method, system, device, medium, and application for constructing a diagnostic model for male infertility, belonging to the fields of male infertility diagnosis, drug screening, and assisted reproductive technology. In this invention, by simultaneously and quantitatively analyzing the pH value, mitochondrial G-quadruplex structure level, and β-galactosidase activity within a single sperm cell, and integrating routine clinical sperm testing parameters and age, a high-precision diagnostic model for male infertility and a drug screening system are established, providing a reference for assisted reproduction to meet practical needs.

The present disclosure relates to methods and kits for treatment of biological samples comprising non-sperm cells and sperm cells. The present disclosure relates more particularly to methods and kits for treatment of forensic samples comprising non-sperm cells and sperm cells.

The invention encompasses methods for reducing the proportion of sperm cells with abnormal morphology, and unviable sperm cells, in a sperm cell population using flow-cytometric processing. The methods generally entail staining the sperm cells in the population; irradiating the sperm cells in the population; detecting fluorescence emitted by the sperm cells in the population in response to the step of irradiating; differentiating the sperm cells in the population based on orientation or viability; and then collecting a subpopulation of oriented or viable sperm cells.

The invention relates to the technical field of biological medicines, and discloses an application of a plant extract composition in preparation of a medicine for improving DNA damage of testicular spermatogenic cells, and the plant extract contains a herba epimedii extract and a rhizoma polygonati extract. The invention provides new application of the composition containing the herba epimedii extract and the rhizoma polygonati extract in preparation of the medicine for improving DNA damage of the testicular spermatogenic cells, the composition can remarkably improve the DNA damage of the testicular spermatogenic cells of mice caused by cyclophosphamide, recover the multiplication capacity of the mice, inhibit apoptosis of the mice and relieve spermatogenic function damage caused by cyclophosphamide, and the composition can be used for preparing the medicine for improving the DNA damage of the testicular spermatogenic cells of the mice. And the combined administration shows a synergistic effect instead of a simple additive effect, and the drug effect is obviously superior to that of any one of the epimedium extract and the rhizoma polygonati extract which are independently administered.

The invention provides a product related to non-obstructive azoospermia microscopic sperm extraction, application and a method. The invention discloses application of a reagent for detecting clinical information in preparation of a non-obstructive azoospermia microscopic sperm extraction product. The clinical information comprises age, testis size, testosterone, estradiol E2, androgen T, follicle stimulating hormone FSH, statin B and seminal plasma cell-free DNA motif diversity score. The prediction method comprises the following steps: acquiring clinical information of a to-be-tested object; inputting the clinical information into a prediction model based on machine learning to obtain a probability P of successful microscopic sperm extraction; and according to comparison between the probability P and a reference value, diagnosing or predicting whether the to-be-detected object is suitable for microscopic sperm collection. The prediction method provided by the invention can be used for improving the accuracy of predicting the success rate of microscopic sperm extraction of non-obstructive azoospermia, and has a relatively good clinical application value.

A method for characterizing a mobile particle in a sample includes acquisition of at least one image of the sample during an acquisition period, using an image sensor and formation of a series of images, the series of images comprising at least one image; use of each image of the series of images as input image of a detection convolutional neural network, the detection convolutional neural network being configured to detect the particles and to produce, from each image, an output image on which each detected particle is assigned a distribution of intensity, centered on the particle and extending around the particle; for each detected particle, from each output image, estimation of a position of each detected particle in each image of the series of images; and characterization of each detected particle from the estimation of the position established from each image of the series of images.

Sperm cells can be extracted from a sample by passing the sample through a microfluidic device, capturing images of the sample at a first portion of the microfluidic device, analyzing the images to ascertain whether a sperm cell is present in any given portion of the sample, and using a second portion of the microfluidic device to route only those portions of the sample that include a sperm cell into a container. Notably, the fluid-flow channels in the first portion of the microfluidic device have rectangular roofs, which improves the quality of the captured images. On the other hand, the fluid-flow channels in the second portion of the microfluidic device have curved roofs, which improves the operation of the valves that are used to carry out the selective-routing functions.

The present application relates to the field of biotechnology, and particularly relates to application of L-glutamine in preparation of livestock semen cryoprotectant.The present application provides application of L-glutamine in preparation of livestock semen cryoprotectant, and aims to overcome the shortcomings in the existing semen cryopreservation technology.L-glutamine can effectively reduce the damage of sperm cells in the freezing process, reduce the formation of ice crystals, protect the integrity of the cell membrane, and improve the survival rate, motility and fertilization capacity of the frozen sperm through its excellent antioxidant effect and low toxicity.Meanwhile, L-glutamine has good biocompatibility and strong adaptability, and can be widely applied to the semen cryopreservation of different animal breeds, and provides a safe, efficient and low-toxicity new cryoprotectant selection.

The application discloses a preparation of a biological memristor and application of the biological memristor in sperm DNA breakage detection, and takes supernatant of a sample to be detected, and drops the supernatant on the surface of the memristor; after the memristor is connected with a detection platform, different voltage corresponding electrical signals are outputted, and the normal sperm and the sperm with DNA breakage are distinguished. The application has the characteristics of simple detection process, low cost, high sensitivity and fast response, and can quickly distinguish whether sperm DNA sequence breakage exists in sperm cells of a male patient with infertility.

The invention relates to the technical field of micro-fluidic organ chips, and discloses a micro-fluidic testis organ chip and a culture method, the micro-fluidic testis organ chip comprises a PDMS fluid control layer and a PDMS cell culture layer from top to bottom; the at least three culture units are arranged on the PDMS cell culture layer in the axial direction, each culture unit internally comprises three functional areas, the three functional areas are used for testicular sertoli cell culture, spermatogenic cell culture and basement membrane simulation respectively, and the three functional areas are communicated with one another to form a fluid path; the regulation and control part is arranged on the PDMS fluid control layer, and the regulation and control part is used for regulating and controlling fluid paths which are communicated with one another among the three functional areas; the inoculation and collection part is arranged on the PDMS fluid control layer and is used for carrying out cell inoculation and collection in the three functional areas. The in-vivo testis spermatogenesis microenvironment is highly simulated, the limitation of a traditional 2D cell experiment and an animal experiment can be effectively made up, and a new method and platform are provided for analyzing a spermatogenesis disorder mechanism and screening targeted repair drugs.

The invention relates to the field of microbial fermentation, and particularly discloses a fermentation method and a fermentation device of a polygonatum sibiricum and cistanche deserticola fermentation product. Comprising a shell, a mounting cavity and a culture cavity are formed in the shell, and a sealing door is mounted on the front side of the culture cavity; the placement mechanism comprises a supporting plate arranged in the culture cavity, a culture box is placed at the upper end of the supporting plate, a rectangular insertion block is fixedly connected to the upper end of the supporting plate, a rectangular insertion groove matched with the rectangular insertion block is formed in the lower end of the culture box, and an annular flow dividing cavity is formed in the culture box; the annular flow dividing cavity is communicated with the inner bottom of the culture box through a plurality of flow dividing holes; and a gas circulation movement mechanism. According to the fermentation method, lactobacillus rhamnosus is adopted to ferment the rhizoma polygonati and cistanche extracting solution, the number and quality of spermatogenic cells are improved, the fermentation device is matched with nitrogen bubble supply, movement of fermentation liquor is promoted, the uniformity of fermentation temperature and fermentation quality is guaranteed, and therefore the fermentation efficiency is guaranteed.

A composition includes a contraceptive chimeric virus-like particle with an antigenic carrier domain and one or more antigenic regions from a sperm cell in the antigenic carrier domain, with the antigenic carrier domain including human papillomavirus L1 capsid protein and the antigenic regions including one or more structural elements of the Catsper ion channel complex. When administered to a patient, the contraceptive vaccine stimulates production of anti-sperm antibodies that, upon binding to a sperm cell, inhibit the sperm cell's motility and thus inhibit the ability of the sperm cell to fertilize an egg cell. The induced immunoinfertility of the composition can be reversed for brief or extended lengths of time by overdosing the patient with a reversal agent lacking the antigenic carrier domain but having a protein sequence substantially identical to that of the one or more antigenic regions to sequester the anti-sperm antibodies.

The invention discloses a kit and a method for combined detection of sperm cell active oxygen and DNA fragment index, and belongs to the technical field of reproductive biology and clinical diagnosis. The kit comprises reactive oxygen dye, acridine orange dye, acidizing fluid and buffer solution. According to the invention, a specific fluorescent dye is combined with a sperm DNA fragment detection method to obtain a method capable of jointly detecting the sperm cell DNA fragment index and the active oxygen, the detection method can be used for simultaneously detecting in the same tube buffer system, and signals among detected items are not influenced. By adopting the detection method disclosed by the invention, the sperm cells with high active oxygen, high DFI and extremely poor quality (ROS + DFI +) and low active oxygen, low DFI and extremely good quality (ROS-DFI-) can be obviously detected, and clinical research and screening of sperm cells with higher quality and distinguishing of poorer sperm cells can be facilitated.

This invention provides a sperm cell counting detection plate, including a main frame. Auxiliary blocks are fixed to the four corners of the outer side of the upper surface of the main frame. A cover plate is rotatably connected to the top rear end of the main frame via a hinge. A coverslip is fixed to the middle of the cover plate, and fixing blocks are fixed to the four corners of the bottom outer side of the cover plate. A glass slide is fixed to the middle of the main frame, and a main storage cylinder is fixed to the middle of the glass slide. Secondary storage cylinders are fixed to the left and right ends of the middle of the glass slide. An inner frame is fixed to the outer side of the inner frame, and a return spring and a contact plate are embedded inside the inner frame. This invention can achieve multiple sealing protection for the semen in the main storage cylinder, effectively preventing sperm leakage caused by device shaking after semen is added, making subsequent observation and counting more convenient.

This patent discloses a cryopreservation solution and method for improving the sperm survival rate of Penaeus monodon, comprising a base solution and combined cryoprotectants. The base solution is a Penaeus monodon sperm cell culture medium containing Penaeus monodon serum; the combined cryoprotectants include permeable and non-permeable cryoprotectants. By using Penaeus monodon sperm cell culture medium instead of traditional calcium-free saline as the base solution for cryopreservation, an optimized environmental basis is established for cryopreservation, significantly improving cryoprotection efficiency. The cryopreservation method provided in this application enables Penaeus monodon sperm to achieve a survival rate of over 89% after 10 months of cryopreservation, achieving long-term stable preservation of sperm under ultra-low temperature conditions; its key biological activity indicators show no significant difference compared to before cryopreservation, successfully achieving artificial insemination. This provides key technical support for the long-term preservation of Penaeus monodon germplasm resources and lays the foundation for the construction of germplasm banks.

The invention discloses a sperm cell detection-based processing method and a storage medium, a microscope camera is used to shoot sperm to be detected at fixed time intervals, and an improved YOLO target detection model is used to carry out sperm cell detection, so that accurate detection of sperm cells is realized; according to the method, sperm cells are tracked by using a sperm cell tracking model, sperm cell information is recorded into a database, data support is provided for subsequent analysis, and a method for judging the motion trail and length of target sperm cells is established according to the sperm cell information database. According to the method, the number of the sperm frame images is set, the motion trail length corresponding to the number of the sperm frame images and the sperm cell activity degree judgment threshold value are set, the sperm activity degree can be objectively evaluated, a scientific basis is provided for sperm quality evaluation, the sperm quality can be efficiently and accurately evaluated, and important data support is provided for reproductive medicine research.

The invention discloses a method and a system for evaluating male fertility by analyzing meiosis recombination rate. The method comprises the following steps: acquiring a diploid biological sample and a plurality of monomer sperm cells of a subject; respectively determining the diploid and haploid genotypes on the heterozygous loci through sequencing; identifying and filtering meiosis crossover events for each sperm cell based on the genotype data, thereby calculating a quantitative crossover rate; the crossover rate is finally compared to a reference crossover rate from a normal fertility population to assess the fertility of the subject. The key point of the invention lies in that it is revealed for the first time that reduction of meiosis crossover rate is a potential quantitative biomarker of'uniform cell energy deficiency 'in male germ cell lines, and the method can more deeply, objectively and accurately evaluate the internal health status of sperms by measuring the core development event. The kit is especially suitable for diagnosis and risk assessment of asthenospermia and monitoring of infertility treatment effect.

The present invention relates to novel compositions, methods of making the novel compositions, and their use in increasing the viability, survival and longevity and fertility of sperm cells.

The invention relates to a flow cytometry installation, in particular for the processing of spermatozoon-type cells and in particular for the sexing of animal semen, comprising an optical detection system (3) configured to differentiate, according to predetermined parameters, a plurality of cells (26) in a jet of such cells (11) directed towards a differentiation zone (17), and an imaging system (40) configured to visualize the jet comprising the differentiated cells at the level of the differentiation zone and to determine representative information of a time at which a drop containing an identified cell should be generated, and then sorted;with the optical detection system (3) which is provided with a first light radiation source (12) configured to emit a light beam (16) focused on the differentiation zone (17), at least two detection elements (14) configured to detect each a quantity of light re-emitted by the cells (26) which are illuminated in different directions of re-emission, and at least two collection elements (15) which are focused, on a first side, on the differentiation zone (17) and on a second side opposite to the first side, on the respective detection elements (14);the installation being characterized in that at least one of the two collection organs (15) is formed by a lens (60, 70) having a cut edge (61, 71) and in that the imaging system (40) is provided with a camera (41) and a second light source (42), distinct from the first light source (12), which is arranged and configured so as to generate a viewing ray (43) directed towards the differentiation zone (17) and the camera. Figure for the abbreviation: Figure 3;

This invention relates to a male gamete protection gene S19g-A7 and its application in improving rice hybrid fertility and hybrid rice breeding. This invention identifies a male gamete protection gene S19g-A7 at the S19 locus of interspecific hybrid sterility in rice, whose encoded amino acid sequence is shown in SEQ ID NO.2. Introducing the S19g-A7 gene into Asian rice for functional complementation can break the S19 locus-mediated interspecific hybrid sterility in rice, rapidly creating hybrid compatible lines that overcome interspecific hybrid sterility in Asian and African rice. This provides genetic resources and breeding methods for utilizing distant hybrid vigor in high-quality rice germplasm breeding, and has significant application value.

The invention provides a model for studying testis diminution, relates to the technical field of biomedicine, and has the technical key points that Papd7 is a key gene for testis growth and development of male mice through specific experiments. Specifically, after Papd7 is knocked out in a mouse body, compared with a control mouse, the weight of a Papd7 deficient mouse is normally developed, testis can only be developed to half of the size, and correspondingly, the sperm density and activity are only half of those of the control group; and the sperm morphology of the defective mouse is abnormal. In mechanism, testis section staining finds that the testis of the knockout mouse become smaller due to the loss of sperm cells in seminiferous tubules; disorder of various hormones is also one of important reasons. Experimental results show that the Papd7 is a key gene for growth and development of the testis of the mouse, the growth defect of the testis of the mouse can be caused by damaging the function of the Papd7 in the mouse through a CRISPR-Cas9 technology, and the Papd7 is a useful model for researching the testis reduction.

Provided herein are methods and compositions for the detection of silent carriers of chromosomal deletion alleles in a human subject using haploid cells (e.g., sperm cells or egg cells) derived from the subject. The methods provided herein allow for the detection of silent (2+0) carriers of SMA, where the individual has a deletion of the SMN1 gene on one chromosome 5 homolog and two or more copies of the SMN1 gene on the other chromosome 5 homolog.

Rising global temperatures negatively impact crop yields by inhibiting seed and fruit production, primarily due to the vulnerability of pollen tube (PT) delivery of sperm cells to ovules under heat stress (HS). Two in vivo assays were developed to investigate how various HS conditions affect pollen-pistil interactions in Arabidopsis, revealing that HS impairs multiple stages of PT growth: pollen germination, growth in the transmitting tract, emergence from the transmitting tract, and ovule targeting, and seed formation. Increased temperatures progressively halted PT growth earlier in its journey toward the ovules. This disruption of pollen-pistil interactions may contribute to reduced crop yields under HS. Additionally described are expression cassettes designed to generate heat-tolerant plants by overexpressing LLG, offering a potential solution to mitigate the adverse effects of heat stress on crop production.

The invention relates to a flow-cytometry apparatus, particularly for processing sperm cells and in particular for sexing animal semen, comprising an optical detection system (3) configured to differentiate, according to predetermined parameters, between a plurality of cells (26) in a jet of such cells (11) directed towards a differentiation area (17), and an imaging system (40) configured to image the jet containing the differentiated cells in the differentiation area and determine information representative of a time at which a drop containing an identified cell must be generated, to then be sorted; the optical detection system (3) is provided with a first light source (12) configured to emit a light ray (16) focused on the differentiation area (17), with at least two detection members (14) configured to each detect an amount of light re-emitted by the cells (26) that are illuminated in different re-emission directions, and at least two collection members (15) that are focused, on a first side, on the differentiation area (17) and, on a second side opposite the first side, on the respective detection members (14); the apparatus being characterized in that at least one of the two collection members (15) is formed by a lens (60, 70) having a cut edge (61, 71) and in that the imaging system (40) is provided with a camera (41) and with a second light source (42), distinct from the first light source (12), which is arranged and configured so as to generate an imaging ray (43) directed in the direction of the differentiation area (17) and camera.

The application discloses application of a natural product composition in preparation of medicines for preventing or treating azoospermia. The natural product composition is composed of astragalin and curcumin. The natural product composition composed of astragalin and curcumin can inhibit spermatogenic cell apoptosis by synergistically regulating Nrf2 / HO-1 / HIF-1a, and improve rat azoospermia. Compared with western medicines, the natural product composition has a wider treatment window and smaller toxic side effect, and has application prospects in the medicines for preventing or treating azoospermia.

The invention discloses a method and a system for diagnosing and typing male infertility based on sperm RNA (Ribonucleic Acid) characteristics. The core of the invention lies in a biological discovery that most RNA can be systematically degraded and removed in the sperm maturation process, so that the total RNA content of healthy and mature sperms is in negative correlation with the functional soundness of the healthy and mature sperms. The method comprises the following steps: (a) obtaining a semen sample of a subject; (b) separating and purifying sperm cells from the sample; (c) quantifying the purified sperms, and determining the total RNA content of each sperm cell so as to obtain a first value; and (d) comparing the first numerical value with a preset reference value, and if the first numerical value is obviously higher than the reference value, indicating that the subject has low fertility or infertility (such as asthenospermia). According to the invention, an objective and quantifiable molecular biomarker is provided by quantifying residual'molecular package 'in the sperm maturation process, and a new way is provided for accurate diagnosis, typing and treatment of male infertility.