58 results about "Sperm cell" patented technology

The invention relates to a sperm cell analysis and diagnosis system based on a multi-modal large language model, which comprises a multi-modal data co-processing unit, a cross-modal semantic alignment module, a dynamic diagnosis decision engine and a self-adaptive evolution system, the four-dimensional data processing module is used for synchronously processing microscopic images, motion trail videos, biochemical detection data and four-dimensional input data of medical record texts and comprises a feature selector based on a gating attention mechanism. According to the sperm cell analysis and diagnosis system based on the multi-modal large language model, quantitative analysis of sperm movement chaos features is realized for the first time, a nonlinear dynamic evaluation standard is established, a cross-modal knowledge distillation and meta-learning migration framework is developed, a data annotation dependence bottleneck is broken through, and an interpretable clinical decision support system is constructed; dynamic updating and probabilistic suggestion of diagnosis rules are achieved, semantic analysis of single-cell multi-omics data is achieved, and molecular mechanism research results are converted into clinically available knowledge.

Means for rigorous quantitative assessment of sperm motility are presented, based on a number of movement and morphology parameters measured using various image processing methods. The quantitative assessment thereby achieved is objective and multidimensional, allowing for detailed and repeatable assessment of samples. Deep learning methods are also disclosed for detection and classification of sperm cells (or other types of cells or particles) using neural networks, adapted to work both in good and in poor imaging condition, such as low magnification and resolution. Specific methods for both supervised and unsupervised deep learning approaches are delineated. In a non-limiting disclosure, the methods are particularly adapted to deal with cases of azoospermia, where there is a very low number of sperm cells (most of which do not swim) and a lot of debris in the imaged field of view.

The present disclosure relates to methods of identifying, differentiating between and / or sorting sperm cells, or more particularly, X-chromosome bearing sperm (X-CBS) and Y-chromosome bearing sperm (Y-CBS) cells, in a sperm sample, the methods comprising determining a level of a Reactive Oxidant Species and / or a Cellular Change mediated thereby (ROSCC) in each of a plurality of sperm cells in the sperm sample; and based on the determined ROSCC levels, identifying, differentiating between and / or sorting X-CBS and Y-CBS cells in at least a portion of the plurality of sperm cells. Also described are enriched or purified X-CBS and Y-CBS cells, and uses thereof.

The invention discloses an efficient extraction method of blue fox sperm DNA, and belongs to the technical field of molecular biology. Aiming at the structural characteristics that chromatin of the blue fox sperms is highly condensed, histone is replaced by protamine, and a stable nucleoprotein complex is formed through an intermolecular disulfide bond, the invention provides an efficient DNA extraction method suitable for the blue fox sperms. According to the method, based on the synergistic effect of three chemical reagents, namely SDS, PK and DTT, full lysis of the sperm cells of the blue foxes is achieved, and then efficient enrichment of sperm DNA of the blue foxes can be achieved by combining a conventional DNA extraction method. According to the efficient extraction method of the blue fox sperm DNA provided by the invention, the yield and integrity of the blue fox sperm DNA are remarkably improved, and the limitation that sperm chromatin is difficult to effectively lyse by a conventional method is overcome; the method provides reliable technical support for research on genetic diversity evaluation, population management, molecular breeding and the like of the blue foxes, and has the potential of popularization and application in other high-condensation sperm species.

The application discloses a high-precision sperm morphology screening system and a screening method, and relates to the technical field of sperm cell recognition. Raw semen is obtained and filtered to obtain initial semen; the initial semen is spread into a preset single-layer sperm liquid strip to obtain initial single-layer motile sperm; a first magnification operation is performed on the initial single-layer motile sperm to obtain a first initial single-layer motile sperm image; a second magnification operation is performed on the first initial single-layer motile sperm image to obtain a second initial single-layer motile sperm image; and the second initial single-layer motile sperm image is substituted into a high-precision sperm morphology selection model to obtain target high-quality sperm. Through the preliminary filtration of the raw semen and the secondary screening of the preset single-layer sperm strip spreading method, and by adopting the progressive magnification strategy of the first magnification operation and the second magnification operation, the high-precision sperm morphology screening can be performed when high-quality sperm is selected by using the existing IMSI technology, and the screening efficiency of the high-precision sperm is improved.

The utility model discloses an efficient separator for human seminal spot mixed spots, which relates to the technical field of separators and comprises a centrifugal tube and a detachable sleeve matched with the centrifugal tube, the caliber of the detachable sleeve is consistent with that of the centrifugal tube, and a sleeve cavity for containing biological material evidences is arranged in the detachable sleeve. A plastic filter plate is arranged in the middle of the sleeve cavity, a filter membrane filter layer is arranged at the bottom of the sleeve cavity, and a bottom drainage hole is formed in the bottom of the detachable sleeve. According to the utility model, a two-stage filtering mode of carrier removal filtering and sperm enrichment filtering is constructed in one sleeve, so that a biological material evidence carrier can be efficiently removed, water-soluble substances of human epithelial cells can be quickly filtered, sperm cells can be efficiently separated from related biological material evidences only by using a small amount of biological material evidences, and the operation is simple and convenient. The test speed and efficiency are greatly improved, difficult biological material evidence treatment is simplified, the experiment repeatability is good, the applicability is high, biological material evidence samples are saved, and the cost of related experiments is reduced.

A device for sperm cell selection has a support plate comprising an inlet to receive a sample of sperm cells, an outlet to collect at least some of the sperm cells comprised in the sample, and a microfluidic system therebetween. The microfluidic system comprises a basin receiving a medium and having inlet and outlet zones. The inlet and outlet provide a hydrostatic pressure difference between the zones when medium is added to the basin via the outlet, to provoke a stream of the medium from the outlet zone towards the inlet zone to initiate sperm cell migration from the inlet zone towards the outlet zone. The basin has a zone of reduced width between the zones, to form a rheotaxis zone. The sample of sperm cells undergoes a selection by passing through the rheotaxis zone during migration for collecting quality sperm cells from the outlet zone at the outlet.

This invention discloses a method, system, device, medium, and application for constructing a diagnostic model for male infertility, belonging to the fields of male infertility diagnosis, drug screening, and assisted reproductive technology. In this invention, by simultaneously and quantitatively analyzing the pH value, mitochondrial G-quadruplex structure level, and β-galactosidase activity within a single sperm cell, and integrating routine clinical sperm testing parameters and age, a high-precision diagnostic model for male infertility and a drug screening system are established, providing a reference for assisted reproduction to meet practical needs.

The present disclosure relates to methods and kits for treatment of biological samples comprising non-sperm cells and sperm cells. The present disclosure relates more particularly to methods and kits for treatment of forensic samples comprising non-sperm cells and sperm cells.

The invention relates to a protective liquid formula for improving the survival rate of frozen and thawed sperms of pigs. The protective liquid formula comprises the following components in percentage by mass: 14% of sodium hexadecylsulfonate, 7% of coconut oil monoethanolamide and 79% of distilled water. The preparation method comprises the following steps: 1) weighing 14g of sodium hexadecylsulfonate and 7g of coconut oil monoethanolamide, and dissolving in 79g of distilled water; and 2) uniformly stirring the solution obtained in the step 1) at 60 DEG C until the sodium hexadecyl sulfonate and the coconut oil monoethanolamide are completely dissolved in the distilled water. The protection liquid has the advantages that the protection liquid protects porcine sperm cells, provides a suitable living environment for porcine sperms, improves the oxidative stress resistance and the preservation effect of the porcine sperms, ensures the survival rate of the porcine sperms, and further improves the breeding rate and the breeding benefits of sows; the survival rate, the sperm motility and the acrosome integrity rate of the frozen and thawed sperms of the pigs are obviously improved; the feed additive is simple in proportioning, high in reliability, low in economic cost, high in biological value and convenient to popularize and apply in production, and yolk with extremely high preparation requirements is not used.

The invention consists of methods and compositions for detecting the presence or absence of a DNA aberration by analyzing fluorescence emission characteristics in sperm cells or sperm nuclei, which generally consists of entraining sperm cells or sperm nuclei stained with a DNA selective dye in sheath fluid; exposing the entrained sperm cells or sperm nuclei to electromagnetic radiation; determining a forward fluorescence characteristic and a side fluorescence characteristic of individual events associated with the exposed sperm cells or sperm nuclei; and gating the individual events based on the forward fluorescence characteristic and the side fluorescence characteristic with a criterion.

The invention encompasses methods for reducing the proportion of sperm cells with abnormal morphology, and unviable sperm cells, in a sperm cell population using flow-cytometric processing. The methods generally entail staining the sperm cells in the population; irradiating the sperm cells in the population; detecting fluorescence emitted by the sperm cells in the population in response to the step of irradiating; differentiating the sperm cells in the population based on orientation or viability; and then collecting a subpopulation of oriented or viable sperm cells.

The invention relates to the technical field of biological medicines, and discloses an application of a plant extract composition in preparation of a medicine for improving DNA damage of testicular spermatogenic cells, and the plant extract contains a herba epimedii extract and a rhizoma polygonati extract. The invention provides new application of the composition containing the herba epimedii extract and the rhizoma polygonati extract in preparation of the medicine for improving DNA damage of the testicular spermatogenic cells, the composition can remarkably improve the DNA damage of the testicular spermatogenic cells of mice caused by cyclophosphamide, recover the multiplication capacity of the mice, inhibit apoptosis of the mice and relieve spermatogenic function damage caused by cyclophosphamide, and the composition can be used for preparing the medicine for improving the DNA damage of the testicular spermatogenic cells of the mice. And the combined administration shows a synergistic effect instead of a simple additive effect, and the drug effect is obviously superior to that of any one of the epimedium extract and the rhizoma polygonati extract which are independently administered.

The invention provides a product related to non-obstructive azoospermia microscopic sperm extraction, application and a method. The invention discloses application of a reagent for detecting clinical information in preparation of a non-obstructive azoospermia microscopic sperm extraction product. The clinical information comprises age, testis size, testosterone, estradiol E2, androgen T, follicle stimulating hormone FSH, statin B and seminal plasma cell-free DNA motif diversity score. The prediction method comprises the following steps: acquiring clinical information of a to-be-tested object; inputting the clinical information into a prediction model based on machine learning to obtain a probability P of successful microscopic sperm extraction; and according to comparison between the probability P and a reference value, diagnosing or predicting whether the to-be-detected object is suitable for microscopic sperm collection. The prediction method provided by the invention can be used for improving the accuracy of predicting the success rate of microscopic sperm extraction of non-obstructive azoospermia, and has a relatively good clinical application value.

A method for characterizing a mobile particle in a sample includes acquisition of at least one image of the sample during an acquisition period, using an image sensor and formation of a series of images, the series of images comprising at least one image; use of each image of the series of images as input image of a detection convolutional neural network, the detection convolutional neural network being configured to detect the particles and to produce, from each image, an output image on which each detected particle is assigned a distribution of intensity, centered on the particle and extending around the particle; for each detected particle, from each output image, estimation of a position of each detected particle in each image of the series of images; and characterization of each detected particle from the estimation of the position established from each image of the series of images.

The invention relates to a method of enriching a cell population containing intact and damaged cells in a liquid medium, comprising the following steps: a.) providing the liquid medium containing the cell population, b.) sedimenting the cell population out of the liquid medium, c.) separating the liquid medium and the sedimented cell population, d.) resuspending the sedimented cell population in a suspension solution to produce a cell suspension, e.) admixing the cell suspension with a ferromagnetic material that binds specifically to the damaged cells, f.) incubating the cell suspension admixed with the ferromagnetic material, g.) introducing the cell suspension into a magnetic field in order to separate the bound cells and the unbound cells in the cell suspension, and h.) separating a fraction of the cell suspension enriched with unbound intact cells, to the use of intact sperm cells enriched in the method, and to an apparatus for separating cells bound to magnetic particles in a cell suspension.

The application provides a hidden sperm screening system and method based on magnetic bead capture and fluorescent identification, and is applied to the technical field of magnetic bead fluorescence analysis. A sperm sample is incubated with high-magnetic-saturation first immunomagnetic beads and low-magnetic-saturation second immunomagnetic beads in an incubation module, and the two kinds of magnetic beads are coupled with anti-sperm antibodies and anti-non-sperm cell antibodies respectively. A magnetic field generating module applies an alternating magnetic field to a separation area and synchronously injects sheath liquid, and uses the difference in magnetic response characteristics to retain the complex combined with sperm and to flush away the complex combined with non-sperm cells. A dye introduction module introduces membrane impermeable and permeable dyes under the constraint of a magnetic field, and obtains fluorescent characteristics of membrane-intact and membrane-damaged sperm which can be distinguished. An imaging module performs multi-channel fluorescence imaging, an image analysis module extracts positive events conforming to morphology and fluorescent logic, and finally outputs sperm count parameters and membrane integrity classification parameters synchronously.

The invention relates to a method for enriching a cell population containing intact and damaged cells in a liquid medium, comprising the steps of: a) providing the liquid medium containing the cell population, b) sedimenting the cell population from the liquid medium, c) separating the liquid medium and the sedimented cell population, d) resuspending the sedimented cell population in a suspension solution to produce a cell suspension, e) adding a ferromagnetic material to the cell suspension that binds specifically to the damaged cells, f) incubating the cell suspension containing the ferromagnetic material, g) placing the cell suspension in a magnetic field to separate the bound and unbound cells in the cell suspension, and h) separating a portion of the cell suspension enriched with unbound, intact cells.the use of intact sperm cells enriched in the process and a device for separating cells bound to magnetic particles in a cell suspension.

The present invention relates to a method for analysing DNA fragmentation in a sperm cell by approximating the output of a pre-selected chemical assay of sperm DNA fragmentation. According to a first aspect of the present invention, there is provided a method for analysing DNA fragmentation in a sperm cell by approximating the output of a pre-selected chemical assay of sperm DNA fragmentation, the method comprising:providing an image of the sperm cell, under brightfield and / or phase contrast with a total magnification of 400× to 1000×;evaluating the image of the sperm cell to identify and / or measure a pre-selected biomarker; andapproximating the output of the pre-selected chemical assay of sperm DNA fragmentation of the sperm cell by subjecting the identified and / or measured biomarker to a first machine learning analysis.

Sperm cells can be extracted from a sample by passing the sample through a microfluidic device, capturing images of the sample at a first portion of the microfluidic device, analyzing the images to ascertain whether a sperm cell is present in any given portion of the sample, and using a second portion of the microfluidic device to route only those portions of the sample that include a sperm cell into a container. Notably, the fluid-flow channels in the first portion of the microfluidic device have rectangular roofs, which improves the quality of the captured images. On the other hand, the fluid-flow channels in the second portion of the microfluidic device have curved roofs, which improves the operation of the valves that are used to carry out the selective-routing functions.

The present application relates to the field of biotechnology, and particularly relates to application of L-glutamine in preparation of livestock semen cryoprotectant.The present application provides application of L-glutamine in preparation of livestock semen cryoprotectant, and aims to overcome the shortcomings in the existing semen cryopreservation technology.L-glutamine can effectively reduce the damage of sperm cells in the freezing process, reduce the formation of ice crystals, protect the integrity of the cell membrane, and improve the survival rate, motility and fertilization capacity of the frozen sperm through its excellent antioxidant effect and low toxicity.Meanwhile, L-glutamine has good biocompatibility and strong adaptability, and can be widely applied to the semen cryopreservation of different animal breeds, and provides a safe, efficient and low-toxicity new cryoprotectant selection.

The application discloses a preparation of a biological memristor and application of the biological memristor in sperm DNA breakage detection, and takes supernatant of a sample to be detected, and drops the supernatant on the surface of the memristor; after the memristor is connected with a detection platform, different voltage corresponding electrical signals are outputted, and the normal sperm and the sperm with DNA breakage are distinguished. The application has the characteristics of simple detection process, low cost, high sensitivity and fast response, and can quickly distinguish whether sperm DNA sequence breakage exists in sperm cells of a male patient with infertility.

The invention relates to the technical field of micro-fluidic organ chips, and discloses a micro-fluidic testis organ chip and a culture method, the micro-fluidic testis organ chip comprises a PDMS fluid control layer and a PDMS cell culture layer from top to bottom; the at least three culture units are arranged on the PDMS cell culture layer in the axial direction, each culture unit internally comprises three functional areas, the three functional areas are used for testicular sertoli cell culture, spermatogenic cell culture and basement membrane simulation respectively, and the three functional areas are communicated with one another to form a fluid path; the regulation and control part is arranged on the PDMS fluid control layer, and the regulation and control part is used for regulating and controlling fluid paths which are communicated with one another among the three functional areas; the inoculation and collection part is arranged on the PDMS fluid control layer and is used for carrying out cell inoculation and collection in the three functional areas. The in-vivo testis spermatogenesis microenvironment is highly simulated, the limitation of a traditional 2D cell experiment and an animal experiment can be effectively made up, and a new method and platform are provided for analyzing a spermatogenesis disorder mechanism and screening targeted repair drugs.

Methods for analyzing somatic cell-derived DNA molecules in a semen sample. The methods may involve, individually or in combination, isolating and analyzing select populations of DNA obtained from sperm cells and somatic cells in a semen sample and size-based sorting. The methods further relate to quantitative analysis of somatic cell-derived DNA molecules in a semen sample. The methods described herein also relate to diagnostic methods for detecting prostate cancer, benign prostate hyperplasia (BPH), prostatitis, or testicular cancer in isolated populations of somatic cell-derived DNA obtained from a patient.

The invention relates to the field of microbial fermentation, and particularly discloses a fermentation method and a fermentation device of a polygonatum sibiricum and cistanche deserticola fermentation product. Comprising a shell, a mounting cavity and a culture cavity are formed in the shell, and a sealing door is mounted on the front side of the culture cavity; the placement mechanism comprises a supporting plate arranged in the culture cavity, a culture box is placed at the upper end of the supporting plate, a rectangular insertion block is fixedly connected to the upper end of the supporting plate, a rectangular insertion groove matched with the rectangular insertion block is formed in the lower end of the culture box, and an annular flow dividing cavity is formed in the culture box; the annular flow dividing cavity is communicated with the inner bottom of the culture box through a plurality of flow dividing holes; and a gas circulation movement mechanism. According to the fermentation method, lactobacillus rhamnosus is adopted to ferment the rhizoma polygonati and cistanche extracting solution, the number and quality of spermatogenic cells are improved, the fermentation device is matched with nitrogen bubble supply, movement of fermentation liquor is promoted, the uniformity of fermentation temperature and fermentation quality is guaranteed, and therefore the fermentation efficiency is guaranteed.

A composition includes a contraceptive chimeric virus-like particle with an antigenic carrier domain and one or more antigenic regions from a sperm cell in the antigenic carrier domain, with the antigenic carrier domain including human papillomavirus L1 capsid protein and the antigenic regions including one or more structural elements of the Catsper ion channel complex. When administered to a patient, the contraceptive vaccine stimulates production of anti-sperm antibodies that, upon binding to a sperm cell, inhibit the sperm cell's motility and thus inhibit the ability of the sperm cell to fertilize an egg cell. The induced immunoinfertility of the composition can be reversed for brief or extended lengths of time by overdosing the patient with a reversal agent lacking the antigenic carrier domain but having a protein sequence substantially identical to that of the one or more antigenic regions to sequester the anti-sperm antibodies.

The invention discloses a kit and a method for combined detection of sperm cell active oxygen and DNA fragment index, and belongs to the technical field of reproductive biology and clinical diagnosis. The kit comprises reactive oxygen dye, acridine orange dye, acidizing fluid and buffer solution. According to the invention, a specific fluorescent dye is combined with a sperm DNA fragment detection method to obtain a method capable of jointly detecting the sperm cell DNA fragment index and the active oxygen, the detection method can be used for simultaneously detecting in the same tube buffer system, and signals among detected items are not influenced. By adopting the detection method disclosed by the invention, the sperm cells with high active oxygen, high DFI and extremely poor quality (ROS + DFI +) and low active oxygen, low DFI and extremely good quality (ROS-DFI-) can be obviously detected, and clinical research and screening of sperm cells with higher quality and distinguishing of poorer sperm cells can be facilitated.

This invention provides a sperm cell counting detection plate, including a main frame. Auxiliary blocks are fixed to the four corners of the outer side of the upper surface of the main frame. A cover plate is rotatably connected to the top rear end of the main frame via a hinge. A coverslip is fixed to the middle of the cover plate, and fixing blocks are fixed to the four corners of the bottom outer side of the cover plate. A glass slide is fixed to the middle of the main frame, and a main storage cylinder is fixed to the middle of the glass slide. Secondary storage cylinders are fixed to the left and right ends of the middle of the glass slide. An inner frame is fixed to the outer side of the inner frame, and a return spring and a contact plate are embedded inside the inner frame. This invention can achieve multiple sealing protection for the semen in the main storage cylinder, effectively preventing sperm leakage caused by device shaking after semen is added, making subsequent observation and counting more convenient.