The
flow cell of the present application simultaneously monitors and measures
light absorbance and
fluorescence of particles in a flowing liquid. The
flow cell comprises a housing having a light input face, an
absorbance output face and first and second emission output faces; a fluid flow section within the housing that comprises a bottom funnel through which fluid enters the
flow cell, a core chamber into which fluid flows from the bottom funnel, and a top funnel into which fluid flows from the core chamber, wherein the bottom and top funnels each comprise a first end which extends at an angle to a second end that is wider in
diameter than the first end, and said second end of each is adjacent to and aligned with the core chamber; and a center section within the housing center having a recess formed therein which houses the core chamber of the fluid flow section, wherein said center section comprises a first pair of opposing channels formed in the light input face and the
absorbance output face, respectively, and a second pair of opposing channels formed in the first emission output face and the second emission output face and which are perpendicular to the first pair of opposing channels, and wherein the first pair of opposing channels and second pair of opposing channels are in communication with the core chamber. An apparatus comprising the flow
cell is also provided.