33 results about "Sex selection" patented technology

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

The present invention provides a method of cryopreserving sperm that have been selected for a specific characteristic. In a preferred embodiment, the method is employed to freeze sex-selected sperm. Although the cryopreservation method of the invention can be used to freeze sperm selected by any number of selection methods, selection using flow cytometry is preferred. The present invention also provides a frozen sperm sample that has been selected for a particular characteristic, such as sex-type. In preferred embodiments, the frozen sperm sample includes mammalian sperm, such as, for example, human, bovine, equine, porcine, ovine, elk, or bison sperm. The frozen selected sperm sample can be used in a variety of applications. In particular, the sample can be thawed and used for fertilization. Accordingly, the invention also includes a method of using the frozen selected sperm sample for artificial insemination or in vitro fertilization.

Improved insemination systems particularly adapted to use for sex-selected sperm sorting include systems which achieve superovulation and then multiple embryo production with sexed embryos. These systems combine with other techniques, including techniques for enhanced sheath fluid and other strategies which minimize stress on the sperm cells, and, potentially, a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial application of sperms samples as well as the resulting animals may be achieved.

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

Materials and Methods for the separation of X- and Y-chromosome bearing sperm, for example in a semen sample, are provided. The methods involve contacting the semen sample with a binding agent, such as an antibody, that specifically binds to an antigen that is specific for an X- or Y-chromosome. Kits for use in the methods are also provided.

The invention discloses an onion cytoplasm male sterility SCAR marker, wherein the length of the specific fragment of the SCAR marker is 339bp, and the nucleotide sequence thereof is shown as SEQ ID No.2. The invention further discloses a primer of the SCAR maker, wherein the forward primer is Zt339-u:5'-GTTCTCAAGCAGATTCCGCAC-3', and the reverse primer is Zt339-d:5'-AGGACCAAACCGAGAGTGAGC-3'. Quick and accurate selection for onion cytoplasm fertility can be realized by using the obtained SCAR marker, thereby quickening the seed selection for new breed strain of onion cytoplasm male sterility, and establishing novel onion coenospecies parent selection system; first-filial generation breeds with independent intellectual property are selected and bred, thereby solving the difficult problems that conventional seeds are mainly used in the current production in China and large sums of foreign exchange is used for buying foreign coenospecies.

A heterogeneous inseminate including a first amount of sperm of a first animal and a second amount of sperm of a second animal of the same species, the first amount of sperm and the second amount of sperm sex-selected sperm of the same sex, useful in the in-vivo or in-vitro fertilization of an egg of a female animal of the same species for the production of sex-selected embryos and sex-selected offspring.

The invention provides a selection method of new cold-resistant species of tilapia. The invention is characterized by determining the cold resistance of tilapia of different geographical population and screening the tilapia of different geographical population by adopting the technology such as colony continuous progeny selection, family selection, specialized line selection and the like and using natural screening and artificial low-temperature stress selection as the means, selecting the cold-resistant tilapia of specialized line and creating the selection method of new cold-resistant species of tilapia. The technology has the characteristics of strong pertinency, obvious effect and the like. The overwintering survival rate of the cold-resistant tilapia fries is over 80% and the low-temperature mortality of the tilapia is reduced by more than 15% than that of the common tilapia. The selection method opens up a new technical approach for breeding of cold-resistant species of fish, is suitable for being popularized and applied in fish selection and has great significance and application value in safe overwintering of the cultivated fish.

The present invention provides systems and non-invasive methods for gender selection of eukaryotic organisms More specifically, the invention applies the CRISPR-Cas system as well as any derivatives and fusion proteins thereof for creation of transgenic eukaryotic organisms and for selecting the desired gender of the resulting progeny.

Non-surgical artificial insemination is achieved for sexed and unsexed equines in a commercially practical manner and with dosages of insemination sperm which were not previously thought to be practical for commercial implementation. Practical and field usable techniques for insemination are presented as well as techniques which offer success ratios at level comparable to the existing routine, high dosage unsexed artificial insemination techniques in equines. Improved insemination and sorting systems particularly adapted to use for sex-selected sperm are disclosed.

Intracytoplasmic sperm injection utilizing sex-selected equine spermatozoa to obtain viable sex selected embryos transferable to a recipient female equine mammal to obtain sex selected foals.

Materials and methods for the separation of X- and Y-chromosome bearing sperm, for example in a semen sample, are provided. The methods involve contacting the semen sample with a binding agent, such as an antibody, that specifically binds to an antigen that is specific for an X- or Y-chromosome. Kits for use in the methods are also provided.

The generation of characters within computer animations is currently a labor intensive and expensive activity for a wide range of businesses. Whereas prior art approaches have sought to reduce this loading by providing reference avatars, these do not fundamentally overcome the intensive steps in generating these reference avatars, and they provide limited variations. According to the invention a user is provided with a simple and intuitive mechanism to affect the weightings applied in establishing the physical characteristics of an avatar generated using an inheritance based avatar generator. The inheritance based generator allowing, for example, the user to select a first generation of four grandparents, affect the weightings in generating the second generation parents, and affect the weightings in generating the third generation off-spring avatar from these parents. Accordingly the invention provides animators with a means of rapidly generating and refining the off-spring avatar to provide the character for their animated audio-visual content.

A method for influencing sex selection in artificial insemination, the method characterised by the exposure of unsexed semen to antibodies to sex specific antigens present on spermatozoa carrying chromosomes of an undesired sex, whereby the spermatozoa to which the sex specific antigens are bound are generally unable to effect fertilisation of an egg, such that fertilisation is likely to be effected by spermatozoa carrying chromosomes of a desired sex.

A PLS-based multi-disturbance integrated gene selection and tumor-specific gene subset recognition method features that different disturbance mechanisms are introduced to analyze gene selection of multi-disturbance integrate according to characteristics of tumor microarray data. Based on this framework, a new integrated gene selection method based on PLS is developed by using PLS polygenic metrics. On the one hand, the method of the invention is based on the whole effect of the subset, and can quickly identify the genes with differential expression, and also can identify the genes with weak differential expression signal; on the other hand, the method of the invention is based on a multiple disturbance mechanism, and can identify a series of different subsets of genes with small length andstrong discrimination ability. Therefore, the method of the invention can identify a series of different gene subsets and weakly differentially expressed genes, through which the specific expressionpattern of tumor genes can be more comprehensively recognized.

The method and process for sex selection based on a quantitative DNA measurement in spermatozoa with damaged plasma membrane.

A heterogeneous inseminate including a first amount of sperm of a first animal and a second amount of sperm of a second animal of the same species, the first amount of sperm and the second amount of sperm sex-selected sperm of the same sex, useful in the in-vivo or in-vitro fertilization of an egg of a female animal of the same species for the production of sex-selected embryos and sex-selected offspring.

The invention discloses a method for interactive inquiry of a traditional Chinese medicine syndrome factor inquiry voice robot. The method includes the steps: S1, after a user registers or directly logs in, performing gender selection, and inputting basic information of the user; S2, entering an inquiry link of the traditional Chinese medicine syndrome factor inquiry voice robot, selecting by theuser a constitution identification mode and a syndrome factor mode for detection; identifying the user's constitution or / and the syndrome factor mode by the traditional Chinese medicine syndrome factor inquiry voice robot, performing voice interactive inquiry with the traditional Chinese medicine syndrome factor inquiry voice robot by the user, and entering interactive inquiry information into thetraditional Chinese medicine syndrome factor inquiry voice robot, analyzing by a traditional Chinese medicine syndrome factor inquiry voice robot background, an performing constitution judgment and syndrome factor mode integration; S3, displaying a constitution property partial order structure diagram and the syndrome factor mode integration on a traditional Chinese medicine syndrome factor inquiry human-machine interface, and informing a constitution identification result; S4, obtaining, by a doctor, a detailed score report according to a syndrome factor mode identification result, and completing a subsequent diagnosis.

A process to bulk sex-select sperm which can involve such steps as: obtaining a subject sperm sample such as a collection of cells (1); inducing a sex-based differential alteration process for sperm in the sperm sample; presenting associationally active elements near the sperm within at least some of the sperm sample perhaps such as a fluid combination (4); causing such elements to differentially associate with at least portions of the elements based upon a sperm sex-based differential alteration state; acting on the elements together with their associated sperm through a separation modality (5) to bulk separate the sperm according to their differential sex-based properties. One type of associationally active element is potentially magnetic particles or differentially associatable particles (3) which may even be magnetic particles with inherent zeta potential charges that may associationally act and perhaps bind to an opposite zeta potential or other charges that the sperm may differentially acquire or achieve. This may present a method for magnetic separation of X-bearing and Y-bearing sperm perhaps such as having different charged membranes and perhaps such as after sialic acid activation.

The invention discloses a multi-character selective crossbreeding method for sheep suitable for alpine environment, and belongs to the technical field of livestock breeding. The steps of the method: use the Horba sheep local group as the female parent of the F1 generation of hybridization, and the East Fried sheep as the male parent of the F1 generation of hybridization to obtain the F1 generation of hybridization; use the female sheep in F1 as the female parent of the F2 generation of hybridization, and use the female sheep of the F1 generation as the female parent of the F2 generation. The sheep were the F2 generation hybrid male parent, and the second generation F2 hybrid was obtained; the female sheep in F2 was used as the F3 generation hybrid female parent, and the Suffolk sheep was the F3 generation hybrid male parent, and the third generation F3 hybrid was obtained; the female sheep in F3 was used as the F3 hybrid female parent. The female parent of the F4 crossbreed, Dorset sheep was the male parent of the F4 crossbreed, and the four-generation F4 was obtained; the four-generation F4 was used as the female parent and the male parent for cross-fixation, and the target sheep core group was obtained. The beneficial effect of the present invention is to solve the problem of cultivating a high-breeding and grain-saving core group of meat sheep with tender meat, double lambs, fast growth, no horns and stable genetic performance suitable for the alpine environment.

The invention provides an SSR (Simple Sequence Repeat) molecular marker for a thoracic window, which is characterized in that the SSR molecular marker comprises one or more of 13 groups of SSR molecular markers, and the serial numbers of the 13 groups of SSR molecular markers are SSR308, SSR311, SSR313, SSR319, SSR320, SSR323, SSR324, SSR325, SSR405, SSR406, SSR501, SSR503 and SSR504 respectively; the nucleotide sequences of the 13 groups of SSR molecular marker sites are as shown in SEQ ID NO. 1 to 13. According to the SSR molecular marker disclosed by the invention, a technical system is established, so that the blank of the specific molecular marker of the scirpus juncoides is filled, the SSR molecular marker can be used for identifying and analyzing firefly offspring paternal right, genetic diversity, population genetics and germplasm, and a result can be used for sex selection and evolution research of a scirpus juncoides insect; and evaluating artificial feeding, rejuvenation and protection of the scirpus juncoides.

The invention relates to a multi-character seed selection method for livestock and poultry, comprising the following steps: determining the breeding target and breeding traits of the current breeding group according to the current strain, and determining the traits that need to be improved in the current generation; The observed value of each trait determines the elimination weight and the total number of elimination of each trait; the elimination order is determined according to the importance of each trait, and individuals are eliminated from the candidate population in turn according to the importance of the traits, and the traits with lower importance are eliminated first, and the importance Higher traits are lagged behind the elimination, and the traits that most need to be improved are taken as the last traits to be eliminated; after all the traits are eliminated, the final selected group is obtained; when the elimination is performed, different sexes are eliminated separately. Based on the experimental results of simulated seed selection, the invention determines the best seed selection sequence according to the weight value of each character, and greatly improves the breeding effect of key characters.

A method comprising the steps of: 1. optionally subjecting spermatozoa to a treatment step; 2. subjecting the spermatozoa of step 1 to a sex selection step so as to select for either female or male spermatozoa of interest; 3. carrying out a fertilisation step using the spermatazoa of interest of step 2 to produce at least one oocyte, blastocyst, ovum, embryonic cell or embryo; 4. selectively lysing the at least one oocyte, blastocyst, ovum, embryonic cell or embryo of step 3 in the presence of spermatozoa so as to selectively release cellular material from the at least one lysed oocyte, blastocyst, ovum, embryonic cell or embryo; and 5. using the released cellular material in at least one downstream application.