32 results about "Sex selection" patented technology

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

The present invention provides a method of cryopreserving sperm that have been selected for a specific characteristic. In a preferred embodiment, the method is employed to freeze sex-selected sperm. Although the cryopreservation method of the invention can be used to freeze sperm selected by any number of selection methods, selection using flow cytometry is preferred. The present invention also provides a frozen sperm sample that has been selected for a particular characteristic, such as sex-type. In preferred embodiments, the frozen sperm sample includes mammalian sperm, such as, for example, human, bovine, equine, porcine, ovine, elk, or bison sperm. The frozen selected sperm sample can be used in a variety of applications. In particular, the sample can be thawed and used for fertilization. Accordingly, the invention also includes a method of using the frozen selected sperm sample for artificial insemination or in vitro fertilization.

Improved insemination systems particularly adapted to use for sex-selected sperm sorting include systems which achieve superovulation and then multiple embryo production with sexed embryos. These systems combine with other techniques, including techniques for enhanced sheath fluid and other strategies which minimize stress on the sperm cells, and, potentially, a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial application of sperms samples as well as the resulting animals may be achieved.

Materials and Methods for the separation of X- and Y-chromosome bearing sperm, for example in a semen sample, are provided. The methods involve contacting the semen sample with a binding agent, such as an antibody, that specifically binds to an antigen that is specific for an X- or Y-chromosome. Kits for use in the methods are also provided.

The invention discloses an onion cytoplasm male sterility SCAR marker, wherein the length of the specific fragment of the SCAR marker is 339bp, and the nucleotide sequence thereof is shown as SEQ ID No.2. The invention further discloses a primer of the SCAR maker, wherein the forward primer is Zt339-u:5'-GTTCTCAAGCAGATTCCGCAC-3', and the reverse primer is Zt339-d:5'-AGGACCAAACCGAGAGTGAGC-3'. Quick and accurate selection for onion cytoplasm fertility can be realized by using the obtained SCAR marker, thereby quickening the seed selection for new breed strain of onion cytoplasm male sterility, and establishing novel onion coenospecies parent selection system; first-filial generation breeds with independent intellectual property are selected and bred, thereby solving the difficult problems that conventional seeds are mainly used in the current production in China and large sums of foreign exchange is used for buying foreign coenospecies.

The invention provides a selection method of new cold-resistant species of tilapia. The invention is characterized by determining the cold resistance of tilapia of different geographical population and screening the tilapia of different geographical population by adopting the technology such as colony continuous progeny selection, family selection, specialized line selection and the like and using natural screening and artificial low-temperature stress selection as the means, selecting the cold-resistant tilapia of specialized line and creating the selection method of new cold-resistant species of tilapia. The technology has the characteristics of strong pertinency, obvious effect and the like. The overwintering survival rate of the cold-resistant tilapia fries is over 80% and the low-temperature mortality of the tilapia is reduced by more than 15% than that of the common tilapia. The selection method opens up a new technical approach for breeding of cold-resistant species of fish, is suitable for being popularized and applied in fish selection and has great significance and application value in safe overwintering of the cultivated fish.

Non-surgical artificial insemination is achieved for sexed and unsexed equines in a commercially practical manner and with dosages of insemination sperm which were not previously thought to be practical for commercial implementation. Practical and field usable techniques for insemination are presented as well as techniques which offer success ratios at level comparable to the existing routine, high dosage unsexed artificial insemination techniques in equines. Improved insemination and sorting systems particularly adapted to use for sex-selected sperm are disclosed.

Intracytoplasmic sperm injection utilizing sex-selected equine spermatozoa to obtain viable sex selected embryos transferable to a recipient female equine mammal to obtain sex selected foals.

The generation of characters within computer animations is currently a labor intensive and expensive activity for a wide range of businesses. Whereas prior art approaches have sought to reduce this loading by providing reference avatars, these do not fundamentally overcome the intensive steps in generating these reference avatars, and they provide limited variations. According to the invention a user is provided with a simple and intuitive mechanism to affect the weightings applied in establishing the physical characteristics of an avatar generated using an inheritance based avatar generator. The inheritance based generator allowing, for example, the user to select a first generation of four grandparents, affect the weightings in generating the second generation parents, and affect the weightings in generating the third generation off-spring avatar from these parents. Accordingly the invention provides animators with a means of rapidly generating and refining the off-spring avatar to provide the character for their animated audio-visual content.

A method for influencing sex selection in artificial insemination, the method characterised by the exposure of unsexed semen to antibodies to sex specific antigens present on spermatozoa carrying chromosomes of an undesired sex, whereby the spermatozoa to which the sex specific antigens are bound are generally unable to effect fertilisation of an egg, such that fertilisation is likely to be effected by spermatozoa carrying chromosomes of a desired sex.

A PLS-based multi-disturbance integrated gene selection and tumor-specific gene subset recognition method features that different disturbance mechanisms are introduced to analyze gene selection of multi-disturbance integrate according to characteristics of tumor microarray data. Based on this framework, a new integrated gene selection method based on PLS is developed by using PLS polygenic metrics. On the one hand, the method of the invention is based on the whole effect of the subset, and can quickly identify the genes with differential expression, and also can identify the genes with weak differential expression signal; on the other hand, the method of the invention is based on a multiple disturbance mechanism, and can identify a series of different subsets of genes with small length andstrong discrimination ability. Therefore, the method of the invention can identify a series of different gene subsets and weakly differentially expressed genes, through which the specific expressionpattern of tumor genes can be more comprehensively recognized.

The method and process for sex selection based on a quantitative DNA measurement in spermatozoa with damaged plasma membrane.

The invention discloses a method for interactive inquiry of a traditional Chinese medicine syndrome factor inquiry voice robot. The method includes the steps: S1, after a user registers or directly logs in, performing gender selection, and inputting basic information of the user; S2, entering an inquiry link of the traditional Chinese medicine syndrome factor inquiry voice robot, selecting by theuser a constitution identification mode and a syndrome factor mode for detection; identifying the user's constitution or/and the syndrome factor mode by the traditional Chinese medicine syndrome factor inquiry voice robot, performing voice interactive inquiry with the traditional Chinese medicine syndrome factor inquiry voice robot by the user, and entering interactive inquiry information into thetraditional Chinese medicine syndrome factor inquiry voice robot, analyzing by a traditional Chinese medicine syndrome factor inquiry voice robot background, an performing constitution judgment and syndrome factor mode integration; S3, displaying a constitution property partial order structure diagram and the syndrome factor mode integration on a traditional Chinese medicine syndrome factor inquiry human-machine interface, and informing a constitution identification result; S4, obtaining, by a doctor, a detailed score report according to a syndrome factor mode identification result, and completing a subsequent diagnosis.

A process to bulk sex-select sperm which can involve such steps as: obtaining a subject sperm sample such as a collection of cells (1); inducing a sex-based differential alteration process for sperm in the sperm sample; presenting associationally active elements near the sperm within at least some of the sperm sample perhaps such as a fluid combination (4); causing such elements to differentially associate with at least portions of the elements based upon a sperm sex-based differential alteration state; acting on the elements together with their associated sperm through a separation modality (5) to bulk separate the sperm according to their differential sex-based properties. One type of associationally active element is potentially magnetic particles or differentially associatable particles (3) which may even be magnetic particles with inherent zeta potential charges that may associationally act and perhaps bind to an opposite zeta potential or other charges that the sperm may differentially acquire or achieve. This may present a method for magnetic separation of X-bearing and Y-bearing sperm perhaps such as having different charged membranes and perhaps such as after sialic acid activation.

The invention provides an SSR (Simple Sequence Repeat) molecular marker for a thoracic window, which is characterized in that the SSR molecular marker comprises one or more of 13 groups of SSR molecular markers, and the serial numbers of the 13 groups of SSR molecular markers are SSR308, SSR311, SSR313, SSR319, SSR320, SSR323, SSR324, SSR325, SSR405, SSR406, SSR501, SSR503 and SSR504 respectively; the nucleotide sequences of the 13 groups of SSR molecular marker sites are as shown in SEQ ID NO. 1 to 13. According to the SSR molecular marker disclosed by the invention, a technical system is established, so that the blank of the specific molecular marker of the scirpus juncoides is filled, the SSR molecular marker can be used for identifying and analyzing firefly offspring paternal right, genetic diversity, population genetics and germplasm, and a result can be used for sex selection and evolution research of a scirpus juncoides insect; and evaluating artificial feeding, rejuvenation and protection of the scirpus juncoides.

A method comprising the steps of: 1. optionally subjecting spermatozoa to a treatment step; 2. subjecting the spermatozoa of step 1 to a sex selection step so as to select for either female or male spermatozoa of interest; 3. carrying out a fertilisation step using the spermatazoa of interest of step 2 to produce at least one oocyte, blastocyst, ovum, embryonic cell or embryo; 4. selectively lysing the at least one oocyte, blastocyst, ovum, embryonic cell or embryo of step 3 in the presence of spermatozoa so as to selectively release cellular material from the at least one lysed oocyte, blastocyst, ovum, embryonic cell or embryo; and 5. using the released cellular material in at least one downstream application.

The invention relates to a mouse in-vitro fertilization breeding sex selection method, which comprises the following steps: inserting a luciferase gene into a Y chromosome of a male mouse by utilizing a gene editing technology, and carrying out embryo injection and transplantation to obtain a Lu-Y male mouse through gene identification; adding a substrate of luciferase into a sperm preserving fluid of a Lucy-Y male mouse, where Lucy-Y sperms emit fluorescence with the wavelength of 560 nm, and normal X sperms do not have fluorescence; sorting lucc-Y sperms and normal X sperms through a flow cytometry sorter; observing the sorted luc-Y sperms and normal X sperms by using a fluorescence microscope; judging the proportion of Luc-Y sperms in normal X sperms through fluorescence; and if the proportion exceeds the standard, putting the normal X sperms into the flow cytometry sorter again for sorting until the purity of the normal X sperms reaches the standard. According to the invention, the flow sorting result can be conveniently and intuitively judged through naked eyes, and the screening precision of the X sperms and the Y sperms of the mice is effectively improved.