4 results about "Sex selection" patented technology

The invention provides a sex-linked molecular marker-assisted all-male silurus asotus breeding method, which belongs to the technical field of silurus asotus sex breeding, and comprises the following steps: step 1, screening a molecular marker SSR-Y1 linked with silurus asotus Y chromosome, and the gene base sequence of the molecular marker SSR-Y1 is as shown in SEQ ID NO: 1; 2, designing a specific primer pair SSR-Y1: F: 5 '-ATGTCGATCCGAGACCT-3' and a specific primer pair SSR-Y1: R: 5 '-TGCAGATCGTCGATAGG-3', and carrying out amplification on the specific primer pair SSR-Y1: F: 5 '-ATGTCGATCGAGACCT-3'; 3, extracting the genome DNA of an individual to be detected, and amplifying the SSR-Y1 marker through PCR (Polymerase Chain Reaction); 4, judging the gender according to an amplification product, wherein male individuals show 350bp bands, and female individuals do not have bands; and 5, selecting a male parent with the genetic sex of XY, and hybridizing the male parent with the common female XX to obtain an all-male offspring XY. According to the invention, the SSR-Y1 marker is adopted, and the SSR-Y1 marker has the advantages of strong specificity, high accuracy, no consumption of a large amount of manpower and low cost; the genetic sex of the catfish is rapidly identified through PCR amplification, the production process of unisexual fish fries of the catfish is simplified by means of a molecular marker-assisted breeding technology, the breeding speed is increased, and the cost is saved.

This invention belongs to the field of plant sex identification technology, and particularly relates to the application of a molecular marker in identifying the sex of *Zephyranthes* plants. The molecular marker is linked to the Y chromosome of *Zephyranthes* plants and comprises a sequence having at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity with SEQ ID NO:1. The molecular marker provided by this invention can be tightly linked to the Y chromosome of *Zephyranthes* plants, thereby achieving the purpose of early and rapid sex detection. This has significant practical implications for sex selection and proportioning of ornamental garden plants, understanding and protecting endangered *Zephyranthes* germplasm resources, and improving the effectiveness of ex-situ and in-situ conservation management.

Aspects of this invention relate to a novel monoclonal antibody specifically designed to exhibit selective binding affinity towards antigens present predominantly in X chromosome sperm. The antibody is engineered for applications to sex select sperm for cattle breeding purposes.

This invention discloses an intelligent fish fry size, color, and sex selection system based on morphometric feature recognition. The system includes a fry batching module, a diversion and conveying module for processing the fry fed through the batching module, and a fry feature and size discrimination module located above the diversion and conveying module. The fry feature and size discrimination module provides the diversion basis by acquiring fry feature information from fry images. The module includes a shell, a high-definition camera and lighting embedded in the shell facing the diversion slide, and an image processing component installed inside the shell. This invention designs a multi-angle screening system for fry size, body color, and sex, overcoming the three key difficulties in fish fry breeding: size selection, color selection, and sex selection. It effectively solves problems such as cannibalism between larger and smaller fish, selection of ornamental fish, and sex selection at the fry stage, accelerating the artificial breeding process and improving production efficiency.