The invention relates to the field of
enzyme inhibitor screening methods, and discloses a
sialidase inhibitor time-resolved
fluorescence screening method based on a thermally activated delayed
fluorescence probe, the
sialidase inhibitor time-resolved
fluorescence screening method comprises a first reaction solution, a second reaction solution and a
diluent, the first reaction solution is a
sialidase stock solution, the second reaction solution is a DMSO solution of the probe, and the
diluent is a DMSO solution of the probe. The
diluent is a
sodium acetate solution with the pH value of 5.0. After the probe reacts with sialidase, the
fluorescence intensity can be remarkably enhanced, and
microsecond-level fluorescence lifetime is accompanied. If the
system contains the sialidase inhibitor, the
fluorescence intensity signal is reduced, but the fluorescence lifetime is still long. Therefore, by setting the gating time of fluorescence
signal acquisition, the interference of short-life strong fluorescence signals brought by the to-be-screened drugs can be eliminated, and then time-resolved fluorescence screening is realized. The method is simple and rapid, the probe molecules do not contain
lanthanide elements, the cost is low, the
toxicity is avoided, the interference of short-life strong fluorescence signals can be avoided, the
false positive rate is low, the accuracy rate is high, and the applicability is wide.