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35 results about "Selective staining" patented technology

Determination of sperm concentration and viability for artificial insemination

A method for the simultaneous determination of the total concentration of sperm cells and the proportion of live sperm cells in a semen sample is provided. By selective staining of live, dead and / or dying sperm cells, for example using one or more fluorochromes, the detection means can distinguish between live, dead and / or dying sperm cells, for example by detection of the fluorescent response from the sperm cells. The selective staining may be performed at room temperature and in concentrations below standard concentrations. The determination may be performed by selective counting of cells of each fluorescent quality for example by using a flow cytometer having internal concentration standard means. Furthermore, the likelihood of fertilizing a female animal with an insemination dose, taken from a semen sample having a known total concentration of sperm cells and a known proportion of live sperm cells, is predicted on the basis of the thus determined total concentration of sperm cells and the proportion of live sperm cells.
Owner:HATTING

Method for detecting neuronal degeneration and anionic fluorescein homologue stains therefor

An anionic fluorescein homologue capable of selectively staining degenerating neurons in brain slices, the method of making the anionic fluorescein homologue, and the method of using the same for detecting neuronal degeneration. Two anionic fluorescein based fluorescent stains are the water soluble tribasic salt of a mixture of the fluorescein isomers 5-carboxyfluorescein and 6-carboxyfluorescein, and the water soluble tetrabasic salt of 5,6 dicarboxyfluorescein.
Owner:SCHMUED LAURENCE C

Diarylamine-based fluorogenic probes for detection of peroxynitrite

Provided herein are improved fluorogenic compounds and probes that can be used as reagents for measuring, detecting and / or screening peroxynitrite. The fluorogenic compounds of the invention can produce fluorescence colors, such as green, yellow, red, or far-red. Also provided herein are fluorogenic compounds for selectively staining peroxynitrite in the mitochondria of living cells. Provided also herein are methods that can be used to measure, directly or indirectly, the presence and / or amount of peroxynitrite in chemical samples and biological samples such as cells and tissues in living organisms. Also provided are high-throughput screening methods for detecting or screening peroxynitrite or compounds that can increase or decrease the level of peroxynitrite in chemical and biological samples.
Owner:VERSITECH LTD

Concentrating Microorganisms in Aqueous Solution Prior to Selective Staining and Detection

Apparatus and methods for detecting a small number of target microorganisms found in a large volume of aqueous solution by diverting small volumes containing fluorescent microorganisms, including target microorganisms, into a smaller specimen of solution, followed by selectively staining the specimen to tag the target microorganisms and detecting the tagged target microorganisms.
Owner:UNIVERSITY OF WYOMING

Cellular analysis of body fluids

Herein is provided a method for cellular analysis on hematology analyzers. In various aspects, the methods provide separation and / or differentiation between red blood cells (RBCs) and white blood cells (WBCs) by utilizing a fluorescent dye to selectively stain WBCs such that they emit stronger fluorescence signals. The method provides optimal detection limits on WBCs and RBCs, thereby allowing analysis of samples with sparse cellular concentrations. As few as one reagent may be used to prepare a single dilution for body fluid analysis, in order to simplify the body fluid analysis. Minimal damage to WBCs is attained using the lysis-free approach described in aspects of the disclosure.
Owner:ABBOTT LAB INC

Preparation method of ophthalmological viscoelastic agent with selective anterior capsule staining function

The invention relates to the technical field of medical biomaterials. Cataract treatment becomes the ophthalmic clinical attention focus and a main treatment means comprises combination of lens extraction and artificial lens implantation. At present, the main clinical problem comprises intraocular pressure increasing caused by a low anterior lens capsule tear success rate and residual of a viscoelastic agent in the eye. The invention provides the preparation method of the ophthalmological viscoelastic agent with the selective anterior capsule staining function. The preparation method comprises adding a Trypan blue staining agent with a concentration of 0.002-0.6% into a material and carrying out high temperature and high pressure sterilization. The use of the staining agent does not change viscoelastic agent gel properties and an in-vitro release rate in 60min is about 15% so that the staining agent can be continuously and slowly released to an operation part so that selective staining is realized and damage to tissue cells is prevented. Based on the characteristic, the viscoelastic agent can be effectively removed through staining agent instruction after an operation.
Owner:SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD

Enumeration of white blood cells

A sample acquiring device for volumetric enumeration of white blood cells in a blood sample comprises a measurement cavity for receiving a blood sample. The measurement cavity has a predetermined fixed thickness. The sample acquiring device further comprises a reagent, which is arranged in a dried form on a surface defining the measurement cavity. The reagent comprises a hemolysing agent for lysing red blood cells in the blood sample, and a staining agent for selectively staining white blood cells in the blood sample. A system comprises the sample acquiring device and a measurement apparatus. The measurement apparatus comprises a sample acquiring device holder, a light source, and an imaging system for acquiring a digital image of a magnification of the sample. The measurement apparatus further comprises an image analyser arranged to analyse the acquired digital image for determining the number of white blood cells in the blood sample.
Owner:HEMOQUE AB

Enumeration of Thrombocytes

A sample acquiring device for volumetric enumeration of thrombocytes in a blood sample is provided which comprises a measurement cavity for receiving a blood sample. The measurement cavity has a predetermined fixed thickness. The sample acquiring device further comprises a reagent, which is arranged in a dried form on a surface defining the measurement cavity. The reagent comprises a haemolysing agent for lysing red blood cells in the blood sample, and optionally a staining agent for selectively staining thrombocytes in the blood sample. A system comprises the sample acquiring device and a measurement apparatus. The measurement apparatus comprises a sample acquiring device holder, a light source, and an imaging system for acquiring a digital image of a magnification of the sample. The measurement apparatus further comprises an image analyser arranged to analyse the acquired digital image for determining the number of thrombocytes in the blood sample.
Owner:HEMOQUE AB

Selective chromophores

The present invention relates to chromophores for selective staining of cells or cell parts comprising a fluorescent chromophore of the general formulae I The invention further comprises a method for cell sorting, a method for cell targeting, as well as a method for cell identification.
Owner:WESTMAN GUNNAR +2

Fluorescent dye probe for latent fingerprint detection as well as preparation method and application of fluorescent dye probe

The invention relates to a fluorescent dye probe for latent fingerprint detection as well as a preparation method and application thereof. The core chemical structural formula of the probe is as shown in a formula I defined in the description, wherein R1 is an aromatic ring (including aromatic heterocyclic ring) compound or methyl, and at least one of R2 and R3 is a hydrophilic group. The functional fluorescent dye probe molecule provided by the invention can be used for selectively dyeing DNA and latent fingerprints in latent fingerprints, then the latent fingerprints on the surfaces of various objects can be shown by using a method of exciting light with different emission wavebands after being dyed by virtue of portable excitation of light sources with various wavebands and a digital single lens reflex camera, and a high-definition latent fingerprint fluorescence image is obtained.
Owner:SHANGHAI NORMAL UNIVERSITY

Flourescent dot counting in digital pathology images

Fluorescence in situ hybridization (FISH) enables the detection of specific DNA sequences in cell chromosomes by the use of selective staining. Due to the high sensitivity, FISH allows the use of multiple colors to detect multiple targets simultaneously. The target signals are represented as colored dots, and enumeration of these signals is called dot counting. Using a two-stage segmentation framework guarantees locating all potential dots including overlapped dots.
Owner:SONY CORP

Selective tinting method

ActiveUS9677222B2Fast and economical mannerTinting selectivityPattern printingDyeing processChemistrySelective staining
A selective dyeing method is used for dyeing a substrate, selectively within a first exposed surface portion of said substrate. For this purpose, the substrate consists of a material that is impervious to a dye with the exception of the first portion of the exposed surface. In particular, the impervious material can form a layer which covers a base portion of the substrate in a second portion of the exposed surface. The substrate is heated such that the dye penetrates a pervious material which constitutes the first portion of the exposed surface. The method is particularly useful for eliminating light diffused by the walls of a multilayer structure which is supported by means of ocular glass.
Owner:ESSILOR INT CIE GEN DOPTIQUE

Bistrifilate-based fluorogenic probes for detection of superoxide anion radical

The invention provides fluorogenic compounds and probes that can be used as reagents for measuring, detecting and / or screening superoxide. The fluorogenic compounds of the invention can produce fluorescence colors, such as green, yellow, red, or far-red. The invention further provides fluorogenic compounds for selectively staining superoxide in the mitochondria of living cells. The invention also provides methods that can be used to measure, directly or indirectly, the presence and / or amount of superoxide in chemical samples and biological samples such as cells and tissues in living organisms, and a high-throughput screening methods for detecting or screening superoxide or compounds that can increase or decrease the level of superoxide in chemical and biological samples.
Owner:VERSITECH LTD

System and method for generating selective stain segmentation images for cell types of interest

An image analysis system and method to generate selective stain segmentation images for at least one cell type of interest within a stained tissue image are presented. Cell membranes in the tissue image are detected to generate a corresponding membrane mask image. Cell nuclei are classified to generate a classified cells image. Selective stain segmentation images are generated for the at least one cell type of interest based on the membrane mask image and the classified cells image.
Owner:VENTANA MEDICAL SYST INC

Method and system for selectively staining dental composite resin

A method and system for selectively staining dental composite materials includes an applicator (104) and a disclosing agent (102). In one embodiment, the applicator (104) provides the disclosing agent (102) to a tooth surface and a composite filling of the tooth. The composite filling is selectively stained to reveal the presence and location of the composite filling. In another embodiment, the tooth surface is selectively stained to reveal the presence and location of the composite filling.
Owner:CHIU PAUL C

Method and apparatus for separating shaped compositions from composition of complex biological liquid sample

Formed constituents in an aqueous based fluid biologic material sample are separated from the aqueous constituent of the sample, and are concentrated in an examining instrument's focal plane where they can be examined under magnification. Examples of fluids that can be analyzed in this fashion include urine; cerebrospinal fluid; pleural fluid; ascites; fluids aspirated from cysts such as thyroid and breast cysts; cytologic specimens which have been placed in an aqueous fluid; platelet-rich plasma; and the like. The sample is placed in a chamber having a layer of a hydrophilic hydrogel covering a surface of the chamber. An opposite surface of the chamber is transparent, and may be formed by a microscope slide cover slip, or the like. The volume of hydrogel in the chamber is sufficient so that, when the hydrogel absorbs essentially all of the aqueous fraction of the sample, the hydrogel will expand and fill the chamber. The capture surface of the expanded hydrogel is preferably planar, and any formed constituents in the sample will be captured on the capture surface of the hydrogel layer, and will not be absorbed into the hydrogel. Formed constituents, such as: cells; bacteria; crystals; protozoa; ova; parasites; and the like, can be differentially highlighted by use of labeled antibodies, selective stains, or the like, so as to enable optical examination and differentiation of various formed constituents which may be in the sample. Formed constituents may be harvested from the capture surface of the expanded hydrogel layer for more detailed examination and analysis. The capture surface of the hydrogel may be provided with a plurality of beads for use in locating the capture surface with an optical scanning instrument, and for re-establishing previously scanned fields of view.
Owner:斯蒂芬·C·沃德罗 +2

Method for determining coating thickness on coated contact lenses

The invention provides a method for imaging and thickness determination of coatings on coated contact lenses. The method comprises selectively staining a negatively-charged-groups-containing coating over the lens body of a coated contact lens by immersing the coated contact lens in an aqueous solution comprising a fluorescently-labeled polycationic polymer and having a pH of from about 6.5 to 8.0; orthogonally cutting the selectively-stained coated contact lens; and determining the thickness of the coating on the coated contact lens. In addition, the invention provides a method for selecting a candidate coating material comprising negatively charged groups for applying a coating with a desired thickness onto silicone hydrogel contact lenses and for optimizing a coating process for producing coated silicone hydrogel contact lenses with a desired thickness coating thereon.
Owner:ALCON INC

Bistrifilate-based fluorogenic probes for detection of superoxide anion radical

The invention provides fluorogenic compounds and probes that can be used as reagents for measuring, detecting and / or screening superoxide. The fluorogenic compounds of the invention can produce fluorescence colors, such as green, yellow, red, or far-red. The invention further provides fluorogenic compounds for selectively staining superoxide in the mitochondria of living cells. The invention also provides methods that can be used to measure, directly or indirectly, the presence and / or amount of superoxide in chemical samples and biological samples such as cells and tissues in living organisms, and a high-throughput screening methods for detecting or screening superoxide or compounds that can increase or decrease the level of superoxide in chemical and biological samples.
Owner:VERSITECH LTD

Method for evaluating the degree of maturity of corneocytes

Disclosed is an evaluating method for the properties of a corneocyte in a horny layer sample originating in the skin. In the above method, detected and evaluated are selective staining of a cornified envelope in a corneocyte, selective staining of a nuclear component and the antigenicity.
Owner:HIRAO TETABUJI +4

Diarylamine-based fluorogenic probes for detection of peroxynitrite

Provided herein are improved fluorogenic compounds and probes that can be used as reagents for measuring, detecting and / or screening peroxynitrite. The fluorogenic compounds of the invention can produce fluorescence colors, such as green, yellow, red, or far-red. Also provided herein are fluorogenic compounds for selectively staining peroxynitrite in the mitochondria of living cells. Provided also herein are methods that can be used to measure, directly or indirectly, the presence and / or amount of peroxynitrite in chemical samples and biological samples such as cells and tissues in living organisms. Also provided are high-throughput screening methods for detecting or screening peroxynitrite or compounds that can increase or decrease the level of peroxynitrite in chemical and biological samples.
Owner:VERSITECH LTD

System for bright field image simulation

ActiveUS10846848B2Simplifying staining protocolRisk of diagnosisImage enhancementImage analysisImage manipulationBiogenic substance
An image processing system configured to receive a first fluorescence image generically indicating the presence of biological matter; transform the first image into a transformed first image having a first color; for each of the biomarkers, receive a respective second fluorescence image indicating signals emitted by fluorescence stain selectively staining the biomarker, transforming the second images into a respective transformed second images having a respective second color; overlaying and combining the transformed first and second images; storing and / or displaying the combined image as the simulated digital bright field IHC or ISH image. The first image is created using an autofluorescence reference spectrum of the tissue sample or of a similar tissue sample or by using a fluorescence reference spectrum of a first stain which generically binds to biological matter of the tissue sample for spectrally unmixing of a multi-spectral digital image of the tissue sample.
Owner:F HOFFMANN LA ROCHE & CO AG
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