7 results about "Corneocyte" patented technology

The present invention concerns a method for preparing a spherical core-shell skin model, said method comprising the steps of: a) forming microdroplets of an aqueous solution comprising fibroblasts and collagen, in an oil, wherein the formed collagen microdroplets comprise at least one fibroblast per microdroplet; b) gelling at least a portion of the formed collagen microdroplets comprising said at least one fibroblast, thereby obtaining collagen beads comprising at least one fibroblast per bead; c) replacing the oil surrounding the collagen beads comprising said at least one fibroblast with a culture medium, d) contacting (i) the collagen beads comprising said at least one fibroblast with (ii) keratinocytes, and e) incubating (i) said collagen beads with (ii) said keratinocytes for a period of time enabling the formation of an external layer by said keratinocytes around said collagen beads, thereby obtaining a spherical core-shell skin model, the core comprising at least one fibroblast and the shell comprising at least one keratinocyte.

[Problem] There is no gene transduction method with high efficiency and high specificity for skin, subcutaneous tissue, skin ulcer surfaces, and their constituent cells. More generally, achieving high gene transduction efficiency in vivo is challenging. Additionally, it is difficult to morphologically and functionally regenerate skin appendages and to regenerate lost peripheral tissues of the upper and lower limbs (upper arm, forearm, hand, fingers, thigh, lower leg, foot, toes).[Solution] This invention provides solutions to these challenges through a novel AAV (adeno-associated virus) capsid with high gene transduction efficiency and high specificity for skin, subcutaneous tissue, and skin ulcer surface cells, as well as a novel AAV capsid with high gene transduction efficiency and high specificity for epidermal tissue and its constituent keratinocytes. Additionally, the invention provides a novel tissue-specific promoter with high gene expression efficiency and high specificity for epidermal tissue and its constituent keratinocytes, and a pharmaceutical composition that enhances gene transduction efficiency by co-administering steroids alongside in vivo gene transduction. The invention further includes a pharmaceutical composition that promotes the regeneration, neogenesis, and functional enhancement of skin appendages through in vivo gene transduction, thereby compensating for functional impairments of skin appendages. Additionally, it provides a research and development platform for developing methods to regenerate peripheral and missing tissues of the upper and lower limbs, as well as a pharmaceutical composition for achieving tissue regeneration. Thus, the present invention provides methods for overcoming these challenges.

The application discloses a kind of coding genes, the coding gene can encode human source III type collagen alpha 1 chain functional fragment and transdermal peptide TD-1, wherein the coding gene of human source III type collagen alpha 1 chain functional fragment and the coding gene of transdermal peptide TD-1 are expressed in series.The application also discloses a kind of recombinant vectors and a kind of recombinant bacteria, the coding gene and the coding gene of human source 4-proline hydroxylase are contained in the above-mentioned recombinant bacteria.The application also discloses the application of the above-mentioned coding gene, recombinant vector, recombinant bacteria in the production of high transdermal performance recombinant humanization III type collagen.The recombinant humanization III type collagen prepared by the application has similar structure with human source III type collagen, has good biological activity and stability, and has good transdermal ability, has good antioxidant, anti-carbonylation, inhibits tyrosinase activity, inhibits melanin production, promotes keratinocyte migration and promotes the ability of wound repair.

This disclosure relates to media compositions and / or supplements to be added into a medium, and to methods for culturing and / or expanding keratinocytes or keratinocyte-like cells. In another aspect, this disclosure relates to media composition and / or supplements to be added into a medium, and to methods of providing an enriched or selected for population of keratinocytes or keratinocyte-like cells. Keratinocytes or keratinocyte-like cells may be derived from pluripotent stem cells, and more particularly from a differentiated population of pluripotent stem cells.

The present application relates to the use of a plant fermentation product, in particular the use of a Imperata cylindra fermentation product for increasing the expression of genes, promoting the proliferation of collagen and elastin, and increasing the antioxidant capacity. The present application provides an Imperata cylindra fermentation product prepared by two-stage fermentation of an Imperata cylindra water extract with yeast and lactobacillus. The present application also provides the use of an Imperata cylindra fermentation product for preparing a composition for increasing the expression of keratin gene, polykeratin microfilament gene and hyaluronic acid synthase gene, promoting the proliferation of collagen and elastin, and increasing the antioxidant capacity. The Imperata cylindra fermentation product can effectively increase the skin moisturizing capacity, maintain the arrangement of keratinocytes, maintain the integrity of the stratum corneum structure, improve the skin barrier function, make the skin firm and elastic, and prevent skin aging.

Disclosed are bioprinted, three-dimensional, biological skin tissues comprising: a dermal layer comprising dermal fibroblasts; and an epidermal layer comprising keratinocytes, the epidermal layer in contact with the dermal layer to form the three-dimensional, engineered, biological skin tissue. Also disclosed are arrays of engineered skin tissues and methods of making engineered skin tissues.

This application relates to the field of skin care technology, and particularly to a keratin plug removal composition, a keratin plug removal cosmetic, and a method for removing keratin plugs from the skin. The composition of the keratin plug removal component is selected from any of the following combinations: (i) component A and component B; (ii) component A and component C; (iii) component B and component C; and (iv) component A, component B, and component C; component A comprising a fatty acid arginine salt; component B comprising a fatty acid taurine salt; and component C comprising one or more of N-acylaminopropionate, N-acylmethyl taurine taurine, and N-acylglutamate. The keratin plug removal composition and cosmetic have low skin irritation and good keratin plug removal effect.