21 results about "Corneocyte" patented technology

A skin care composition includes a plurality of agents that include at least one retinol derivative that may comprise, for example, hydroxypinacolone retinoate, and at least one bark extract that may comprise, for example, Eperua falcata bark extract, the plurality of agents providing reduced retinol associated inflammation as compared with a composition that includes the at least one retinol and lacks the at least one bark extract. The plurality of agents may also include hydroxy acid, for example, comprising lactic acid. The composition may confer one or a combination of increased skin cell proliferation, improved cellular migration, improved cellular differentiation, an anti-inflammatory retinol genetic signature as demonstrated by via bulk-RNA sequencing of human keratinocytes and restores epidermal disruption and reduce IL-1A and IL-23 inflammatory cytokines in human ex-vivo skin models of chronic inflammation.

The invention discloses a whitening composition as well as a preparation method and application thereof. The whitening composition is prepared from the following components: a tea leaf extract, a lavender flower extract and water, wherein the tea leaf extract, the lavender flower extract and the water are added into the whitening composition. The whitening composition can interact and synergistically improve the autophagy ability of cells, synergistically improve the phagocytosis of melanin in keratinocytes and the removal ability of skin metabolic wastes, can synergistically inhibit the generation of melanin and synergistically improve the expression of miR-137 genes in melanocytes, has a relatively high development prospect of cosmetics with whitening effects, and has a wide application prospect. Moreover, the composition is mild in property, easy to absorb and non-irritant, and can be widely applied to skin care cosmetics.

The invention belongs to the technical field of cosmetics, and discloses a skin-soothing barrier composition capable of being used for children suffering from eczema and application of the skin-soothing barrier composition. The skin-soothing barrier composition is prepared from the following components in parts by mass: 0.05 to 4 parts of fructus schisandrae chinensis extract, 0.1 to 3 parts of tetrahydromethylpyrimidine carboxylic acid, 0.05 to 3 parts of lactobacillus / algae extract fermentation product, 0.05 to 3 parts of glycosylglycerol and 0.3 to 5 parts of capparis spinosa fruit extract. Through the combination of the specific components, a good synergistic effect is generated among the components, the effects of resisting inflammation, relieving, moisturizing, locking water, resisting oxidation and the like are effectively improved, on one hand, oxidative stress injury is relieved, and red-sensitive scorching heat is relieved; on the other hand, dry desquamation is improved by repairing keratinocytes and reinforcing a barrier; therefore, a more comprehensive improvement effect is achieved on symptoms such as repeated attack of eczema, itching and discomfort, dryness and desquamation, red sensitivity and scorching heat of children and the like.

The application discloses a Bifidobacterium breve capable of relieving psoriasis and an application thereof, and belongs to the technical field of microorganisms. The application screens a Bifidobacterium breve CCFM683, and the Bifidobacterium breve CCFM683 has the effect of relieving psoriasis caused by imiquimod, which is specifically embodied in the following aspects: (1) significantly reducing the weight loss of psoriasis mice; (2) significantly improving the pathological characteristics such as scales, thickening and redness of the skin of psoriasis-like mice; (3) regulating the level of skin immune factors of psoriasis-like mice; and (4) significantly inhibiting the proliferation of keratinocytes of the skin of psoriasis mice. The Bifidobacterium breve CCFM683 has great application prospect in the preparation of food or medicine for preventing and / or treating psoriasis.

The present invention concerns a method for preparing a spherical core-shell skin model, said method comprising the steps of: a) forming microdroplets of an aqueous solution comprising fibroblasts and collagen, in an oil, wherein the formed collagen microdroplets comprise at least one fibroblast per microdroplet; b) gelling at least a portion of the formed collagen microdroplets comprising said at least one fibroblast, thereby obtaining collagen beads comprising at least one fibroblast per bead; c) replacing the oil surrounding the collagen beads comprising said at least one fibroblast with a culture medium, d) contacting (i) the collagen beads comprising said at least one fibroblast with (ii) keratinocytes, and e) incubating (i) said collagen beads with (ii) said keratinocytes for a period of time enabling the formation of an external layer by said keratinocytes around said collagen beads, thereby obtaining a spherical core-shell skin model, the core comprising at least one fibroblast and the shell comprising at least one keratinocyte.

[Problem] There is no gene transduction method with high efficiency and high specificity for skin, subcutaneous tissue, skin ulcer surfaces, and their constituent cells. More generally, achieving high gene transduction efficiency in vivo is challenging. Additionally, it is difficult to morphologically and functionally regenerate skin appendages and to regenerate lost peripheral tissues of the upper and lower limbs (upper arm, forearm, hand, fingers, thigh, lower leg, foot, toes).[Solution] This invention provides solutions to these challenges through a novel AAV (adeno-associated virus) capsid with high gene transduction efficiency and high specificity for skin, subcutaneous tissue, and skin ulcer surface cells, as well as a novel AAV capsid with high gene transduction efficiency and high specificity for epidermal tissue and its constituent keratinocytes. Additionally, the invention provides a novel tissue-specific promoter with high gene expression efficiency and high specificity for epidermal tissue and its constituent keratinocytes, and a pharmaceutical composition that enhances gene transduction efficiency by co-administering steroids alongside in vivo gene transduction. The invention further includes a pharmaceutical composition that promotes the regeneration, neogenesis, and functional enhancement of skin appendages through in vivo gene transduction, thereby compensating for functional impairments of skin appendages. Additionally, it provides a research and development platform for developing methods to regenerate peripheral and missing tissues of the upper and lower limbs, as well as a pharmaceutical composition for achieving tissue regeneration. Thus, the present invention provides methods for overcoming these challenges.

The invention relates to a composition with moisturizing and pore shrinking functions and application of the composition in cosmetics, and belongs to the technical field of cosmetics. The composition disclosed by the invention is prepared from the following raw materials in parts by weight: 0.8 to 1.5 parts of epigallocatechin gallate, 0.8 to 1.2 parts of fucosyllactose and 0.15 to 0.2 part of N-sodium hyaluronyl glutamate. In the composition disclosed by the invention, the N-sodium hyaluronyl glutamate is used as a derivative of hyaluronic acid, and a glutamic acid group of the N-sodium hyaluronyl glutamate is similar to a component of a natural moisturizing factor in a cuticle, so that synthesis of lipid between keratinocytes can be promoted, and the arrangement of the cuticle is tighter; the epigallocatechin gallate is used for inhibiting the enzyme activity of MMPs and reducing the degradation of collagen; fucosyllactose can stimulate fibroblasts to synthesize collagen by adjusting flora metabolites, and the three components have a synergistic effect, so that triple effects of three-dimensional moisturizing, pore convergence and collagen regeneration are realized.

The invention relates to a composition for promoting cutin exfoliation. The composition is prepared from cryptomeria fortunei bud extract, lactic acid, epigallocatechin gallate and sodium hyaluronate. The preparation method comprises the following steps: mixing the cryptomeria fortunei bud extract, the lactic acid, the epigallocatechin gallate and the sodium hyaluronate, adding water, uniformly stirring, and standing. The composition provided by the invention solves the problems that the existing keratinocyte exfoliation method is single and strong in irritation, and reduces the adhesive force between keratinocytes by promoting the autophagy of the keratinocytes and promoting the proliferation and activation of the keratinocytes and fibroblasts. Meanwhile, the water content of keratinocytes is increased, normal renewal of cells is promoted, and the skin is shining and fine.

The application belongs to the technical field of biological medicine, and particularly relates to application of a polypeptide in treatment of psoriasis. The amino acid sequence of the polypeptide provided in the application is SEQ ID NO. 1, which can effectively reduce the spleen-body ratio of a psoriasis mouse, reduce the inflammation level in the body, significantly reduce the epidermal thickness of the psoriasis mouse and the inflammatory factors in the lesion tissue, reduce the keratinocyte proliferation, and reduce the recruitment of inflammatory cells to the lesion to release inflammatory factors. The polypeptide provided in the application exhibits certain advantages in aspects of reducing inflammatory response and promoting tissue repair in treatment of psoriasis. The application provides a new method and means for treatment of psoriasis, and promotes the progress and development of medicine.

The invention belongs to the technical field of biological medicines, and particularly relates to application of troxerutin in preparation of medicines for preventing and / or treating skin keratinocyte aging and skin tissue aging. The medicine can delay skin keratinocyte senescence by protecting skin keratinocytes from intracellular active oxygen accumulation, mitochondrial dysfunction and DNA damage caused by ionizing radiation, and the troxerutin can delay skin tissue senescence by protecting skin tissues from skin tissue thinning caused by ionizing radiation. The invention provides a new medicine source for preventing, protecting, delaying and researching the aging of skin keratinocytes and skin tissues. The medicine for controlling the aging of cells and organisms, provided by the invention, is safe, relatively strong in pharmacological action, small in side effect and definite in curative effect.

The application discloses a kind of coding genes, the coding gene can encode human source III type collagen alpha 1 chain functional fragment and transdermal peptide TD-1, wherein the coding gene of human source III type collagen alpha 1 chain functional fragment and the coding gene of transdermal peptide TD-1 are expressed in series.The application also discloses a kind of recombinant vectors and a kind of recombinant bacteria, the coding gene and the coding gene of human source 4-proline hydroxylase are contained in the above-mentioned recombinant bacteria.The application also discloses the application of the above-mentioned coding gene, recombinant vector, recombinant bacteria in the production of high transdermal performance recombinant humanization III type collagen.The recombinant humanization III type collagen prepared by the application has similar structure with human source III type collagen, has good biological activity and stability, and has good transdermal ability, has good antioxidant, anti-carbonylation, inhibits tyrosinase activity, inhibits melanin production, promotes keratinocyte migration and promotes the ability of wound repair.

The invention provides a composition containing a camellia nitidissima extract and application. The composition is prepared from the following components in parts by mass: 0.01 to 3 parts of camellia nitidissima flower extract, 0.01 to 5 parts of camellia nitidissima leaf extract, 0.01 to 1 part of camellia nitidissima essential oil, 0.05 to 2 parts of radix ophiopogonis root extract and 0.01 to 2 parts of dendrobium officinale stem extract. The components of the composition containing the camellia nitidissima extract are synergistic to realize multi-dimensional synergistic repair of a skin barrier: the camellia nitidissima essential oil promotes keratinocyte proliferation and skin renewal, and opens transdermal channels for other components at the same time; the camellia nitidissima flower extract and the camellia nitidissima leaf extract are combined, so that oxidative damage and inflammation are relieved, and cuticle is stabilized; the radix ophiopogonis root extract repairs a skin barrier and provides a storage space for NMF generated by the dendrobium officinale stem extract, and silk protein of the dendrobium officinale stem extract promotes formation of a breathable biological membrane and generates NMF, so that a support is provided for improvement of the skin barrier by the radix ophiopogonis root extract.

This disclosure relates to media compositions and / or supplements to be added into a medium, and to methods for culturing and / or expanding keratinocytes or keratinocyte-like cells. In another aspect, this disclosure relates to media composition and / or supplements to be added into a medium, and to methods of providing an enriched or selected for population of keratinocytes or keratinocyte-like cells. Keratinocytes or keratinocyte-like cells may be derived from pluripotent stem cells, and more particularly from a differentiated population of pluripotent stem cells.

The invention discloses a plant composition for promoting cutin renewal and smoothing skin and application of the plant composition, and belongs to the technical field of cosmetics. The composition is prepared from a pomegranate fruit extract, a kale leaf extract, a dendrobium officinale stem extract and phytosterol according to a specific ratio, wherein the dendrobium officinale stem extract is obtained by combining a choline chloride-oxalic acid eutectic solvent with microjet extraction; in the composition, the pomegranate fruit extract promotes keratinocyte regeneration, the kale leaf extract accelerates old and waste cutin to fall off, and the pomegranate fruit extract and the kale leaf extract cooperate to regulate cutin metabolism; the dendrobium officinale stem extract enhances the skin moisturizing capacity, softens cutin and creates a moist renewal environment; phytosterol can repair the skin barrier and improve the basal layer cell viability. The four components form a complete action chain of deep activation, metabolism regulation and barrier repair, endogenously promote cutin renewal, have the effects of exfoliating, skin smoothing, long-acting moisturizing and barrier repair, are mild and non-irritant, and are suitable for people with sensitive skin.

The present application relates to the use of a plant fermentation product, in particular the use of a Imperata cylindra fermentation product for increasing the expression of genes, promoting the proliferation of collagen and elastin, and increasing the antioxidant capacity. The present application provides an Imperata cylindra fermentation product prepared by two-stage fermentation of an Imperata cylindra water extract with yeast and lactobacillus. The present application also provides the use of an Imperata cylindra fermentation product for preparing a composition for increasing the expression of keratin gene, polykeratin microfilament gene and hyaluronic acid synthase gene, promoting the proliferation of collagen and elastin, and increasing the antioxidant capacity. The Imperata cylindra fermentation product can effectively increase the skin moisturizing capacity, maintain the arrangement of keratinocytes, maintain the integrity of the stratum corneum structure, improve the skin barrier function, make the skin firm and elastic, and prevent skin aging.

The purpose of the present invention is to provide a novel material that promotes the production of collagen and / or hyaluronic acid and / or activates human epidermal keratinocytes. A lactic acid bacterium belonging to the genus Fructobacillus, a culture of the lactic acid bacterium, a culture supernatant of the lactic acid bacterium, and / or an extract of the lactic acid bacterium are useful as active ingredient of a collagen production promoter, a hyaluronic acid production promoter, and an epidermal keratinocyte activator.

Disclosed are bioprinted, three-dimensional, biological skin tissues comprising: a dermal layer comprising dermal fibroblasts; and an epidermal layer comprising keratinocytes, the epidermal layer in contact with the dermal layer to form the three-dimensional, engineered, biological skin tissue. Also disclosed are arrays of engineered skin tissues and methods of making engineered skin tissues.

The invention relates to a pharmaceutical composition with whitening, freckle removing and anti-aging functions and a preparation method of the pharmaceutical composition. Most of existing traditional Chinese medicine preparations such as lonicera hypoglauca flowers, humifuse euphorbia herbs, lysimachia christinae hance and sargentgloryvine stems pay attention to the effects of clearing away heat and toxic materials, promoting blood circulation to subdue swelling and the like. Nano micro-capsule powder prepared through different technological processes is matched with grape seeds and soybean extracts which are remarkable in whitening and anti-oxidation effect and extremely high in flavone content, and vitamin E derived from natural plants is matched, so that the effects of resisting oxidation, blocking tyrosine-tyrosinase series biochemical reactions in skin, inhibiting formation of melanin particles in the skin, improving the skin whitening effect and the skin whitening effect can be achieved, and the skin whitening effect is achieved. The whitening and freckle-removing composition has the advantages that melanin particles are prevented from ascending to epidermal cells to cuticle, melanin is effectively inhibited from being transmitted to keratinocytes, and melanin-containing cells are promoted to fall off, diminish inflammation, remove swelling and fade stains and wrinkles by accelerating the updating speed of skin cells, so that the whitening and freckle-removing effects are finally achieved.

This application relates to the field of skin care technology, and particularly to a keratin plug removal composition, a keratin plug removal cosmetic, and a method for removing keratin plugs from the skin. The composition of the keratin plug removal component is selected from any of the following combinations: (i) component A and component B; (ii) component A and component C; (iii) component B and component C; and (iv) component A, component B, and component C; component A comprising a fatty acid arginine salt; component B comprising a fatty acid taurine salt; and component C comprising one or more of N-acylaminopropionate, N-acylmethyl taurine taurine, and N-acylglutamate. The keratin plug removal composition and cosmetic have low skin irritation and good keratin plug removal effect.

The invention belongs to the technical field of daily-use chemistry, and particularly relates to a keratin regulating composition containing mandelic acid and having skin smoothing and brightening effects and application of the keratin regulating composition. The composition provided by the invention comprises mandelic acid, lactobionic acid, hydroxyethyl piperazine ethanesulfonic acid, lactobacillus / soybean milk fermentation product filtrate and lactobacillus fermentation lysate. According to the application, mandelic acid, lactobionic acid, hydroxyethyl piperazine ethanesulfonic acid, lactobacillus / soybean milk fermentation product filtrate and lactobacillus fermentation lysate are compounded, so that the activity of KLK family proteases, especially kallikrein 5 (KLK5), can be obviously promoted, the decomposition of desmosome between keratinocytes is promoted, epidermal cells lose cohesion and finally naturally fall off, and the effect of promoting the activity of KLK family proteases is achieved. The cutin exfoliation promoting effect is achieved, so that the problems of skin darkness, roughness, pore blockage, acne and the like are solved, the glossiness and fineness of the skin are improved, and healthy and bright skin is achieved.

The invention relates to a skin care composition for external use. The skin care composition for external use comprises nicotinamide and one or more resveratrol salicylate. The composition increases the level of energy in epidermal keratinocytes while mitigating oxidative damage associated with increased ATP production due to administration of nicotinamide. The performance of AQP-3, CLDN-1, and CLDN-4 is also increased, thereby improving barrier function in the skin and increasing moisture retention. Preferably, the composition is used as a mixture or emulsion in the form of cream, lotion, essence or gel.