184 results about "Corneocyte" patented technology

The invention discloses a human-derived cell-factor germinal preparation and the preparation method, relating to the technical filed of the healthcare product. The invention contains lecithin which is the lipid membrane-forming material, cholesterin and active component germinal cell factor, with the proportion by weight between them being 2-6:1-4:1-5; the lecithin is selected from egg yolk lecithin or soybean lecithin; the germinal cell factor is selected from the albumen freeze-dry powder of the culture supernatant of human fibroblasts, placental cells, cuticle cells or dermal papilla cells, with abundant cell factors for the hair growth in the culture supernatant; the germinal cell factors are enveloped in the lipid membrane. The rotary evaporating method is adopted in the process to make the product the lipidosome medicament form which can easily permeate the skin barrier. In addition, the product is frozen and dried into the more stable powder, which needs only to be dissolved by the distilled water containing 2% penetrating enhancer azone while in use. The invention is of easy penetration, high skin retention and good curative effect and free of side effect.

Epidermal keratinocytes not incorporating magnetic particles therein and epidermal keratinocytes incorporating magnetic particles therein were seeded in a 24-well ultra-low-attachment plate, and cultured for one day after a neodymium magnet was placed outside the bottom of the well. As a result, control epidermal keratinocytes not incorporating magnetic particles therein did not adhere to the bottom of the well and did not form an undifferentiated cell sheet. On the contrary, epidermal keratinocytes incorporating magnetic particles therein, while being attracted to the bottom of the well by magnetic force, formed a multilayered cell sheet. This cell sheet could be easily recovered by removing the neodymium magnet placed outside the bottom of the well, then bringing a magnet having a hydrophilic film attached thereto close to the cell sheet from upside and to attract it via this hydrophilic film, and lifting it.

A beauty information providing system, includes: a first computer used in counseling of a customer who visits a merchandise store; a second computer used in counseling of a customer who visits an aesthetic treatment salon; a server connected to the first and second computers via communication networks; evaluation result creating unit which creates results of evaluating a customer's skin based on at least results of analysis on images of corneocytes collected from the customer's skin, results of analysis on enlarged images of the customer's skin, and the customer's answers to a skin evaluation questionnaire; and screen data creating unit which creates screen data for showing the evaluation results, screen data for showing skin care advice suited to the evaluation results, and screen data for showing an aesthetic treatment recipe suited to the evaluation results.

The invention discloses an in-vitro recombined human skin epidermis model.A layer of hemidesmosome protein is arranged on the lower surface of a basal cell layer, the upper surface of the basal cell layer is covered with 6-8 spinous cell layers containing short protruding spinous cells, the upper surface of the spinous cell layers is covered with 3-4 granular cell layers containing transparent granular cells, the upper surface of the granular cell layers is covered with a keratinocyte layer, all the cell layers are obviously separated, and the overall barrier function of a lipid composition is approximate to that of human skin.A preparation method comprises the steps of preparation of matrigel particles, screening and amplification of epidermal stem cells, preparation of the basal cell layer, preparation of the spinous cell layers, preparation of the granular cell layers, and maturation and keratinization of the in-vitro recombined epidermis model.The model is applied to evaluation of cosmetics with the human skin barrier repair effect, screening of plant extract with the human skin barrier repair effect, evaluation and detection of irritation of medical apparatus materials on the human skin and detection of irritation of chemicals on the skin.

The invention discloses a serum-free culture medium used for culturing and amplifying skin keratinocytes in vitro. It adds amino acid, vitamin, hormone, growth gene, trace elements, and antioxidant, thus able to accelerate cell breeding and delay consenescence of the keratinocytes and makes them amplified in vitro by more times. Using the culture medium to make primary culture and subculture needs no trophoblast cells and no extracellular substrates like collagen, etc prepaved at the bottom of the culture bottle to help the keratinocytes adhere to the wall and grow.

The invention discloses a composition for whitening skin, which comprises whitening functional components including persimmon leaf extractive, ginkgo leaf extractive, lotus extractive, crocus sativus flower extractivea pomegranate fruit extractive, dogwood fruit extractive, licoflavone, nicotinamide and ascorbyl glucoside. According to the invention, the whitening functional components can act before generating melanin, in the process of generating melanin, and after generating melanin; the purpose of whitening skin is achieved in multiple aspects of inhibiting gene expression of related rate-limiting enzyme synthesized by melanin, inhibiting enzymatic activity of the related rate-limiting enzyme synthesized by melanin, eliminating oxygen free radicals, reducing generated melanin, limiting melanin to transfer towards horn cells and the like; therefore, the composition for whitening skin disclosed by the invention is safe and effective.

The invention provides a mixed cultivation method of keratinocyte and melanocyte comprising: breaking the skin sample reserving epidermal layer and at least part of corium layer, cold digesting by pancreatin; collecting deposition organization; heat digesting by I type collagenase, collecting cells; primary inoculating, culturing in KGM-Gold medium; subcultring to the cells merge, culturing in KGM-Gold medium to the cells differentiate and stratify; collecting the formed skin sheet. The mixed cultivation method of keratinocyte and melanocyte uses the medium only for skin keratinocyte and melanocyte with which can avoid pollution of the other cells, and can get skin sheet including functional melanocyte and distributing uniformly. The mixed cultivation method of keratinocyte and melanocyte can get large area of skin sheet from quite small area of autologous sample, solve the problem of shortage of the skin source at skin grafting for the patient with large-area skin diseases, and apply in skin diseases of pigment deficiency such as leucoderma and so on.

The invention discloses eye drops with the function of relieving the symptom of xeroma, which are characterized by comprising a recombinant human keratinocyte growth factor-2, a protein protective agent, a thickening agent, an osmotic pressure regulator and a proper buffer system in a certain proportion; the invention also discloses a preparation method of the eye drops. Pharmacological experiments show that the eye drops can delay the rupture time of the lacrimal film, maintain the integrity of the ocular barrier, restore the ocular immunological network and relieve the symptom of the xeroma by restoring the corneal epithelium.

The invention discloses a fibroblast liquid for beauty treatment and a preparation method thereof. The fibroblast liquid contains a cell culture solution, collagens, cell growth factors, amino acids and vitamins, wherein the cell growth factors comprise fibroblast growth factors, epidermal cell growth factors, hepatocyte growth factors and keratinocyte growth factors. The preparation method of the fibroblast liquid comprises the following steps: acquiring umbilical cord tissue hypodermal cells, carrying out primary culture of the umbilical cord tissue hypodermal cells, subcultring the umbilical cord tissue hypodermal cells, collecting the obtained cell culture liquid, and adding glycerin and cytokines to prepare the fibroblast liquid. The fibroblast liquid has the efficacies of epidermal cell metabolism promotion, skin elasticity enhancement, injured epidermal cell restoration, color spot removal and the like; and the preparation method is simple, is easy to operate, and is suitable for the large-scale growth preparation of stem cell liquids.

The invention relates to an epidermal tissue culturing method, an epidermal tissue culture prepared by using the method and application of the epidermal tissue culture in the field of cosmetic dermatology. The epidermal tissue culturing method comprises a step of culturing a culturing mixture of melanin cells and epidermal keratinocytes under the conditions that the quantity of the epidermal keratinocytes is 100 and the quantity of the melanin cells is in a range of 0.01 to 50.

The invention relates to a liposome face-nourishing skin care product, belonging to the technical field of cosmetics. The liposome face-nourishing skin care product comprises the following components in parts by weight: 0.5-8.0 parts of epidermal growth factor (EGF), 0.01-0.1 part of human stem cell growth factor (SCGF), 10.0-10,000.0 parts of hyaluronic acid, 0.003-3000.0 parts of phospholipid, 0.001-10.0 parts of metallothionein, 0.001-10.0 parts of collagen, 0.01-100.0 parts of male larva, and 0.001-200.0 parts of cholesterol. The skin care product provided by the invention has the beneficial effects that the liposome is composed of phospholipid bilayers and contains pure water micro vesicles, and the vesicles can enclose a certain amount of nutrients and essences, are 100 nanometers in mean diameter which is 1 / 200-1 / 300 of the human cell diameter, can easily penetrate through the surface layer of human skin and can be fused with cells, thus exerting efficacy. The skin care product can deeply humidify horny cells and enhance hydration to improve the skin softness, and can provide sufficient nutrients to ensure skin health and increase skin resistance.

The invention relates to a liposome sun-screening skin care product, belonging to the technical field of cosmetics. The liposome sun-screening skin care product comprises the following components in parts by weight: 0.5-8.0 parts of epidermal growth factor (EGF), 0.01-0.1 part of human stem cell growth factor (SCGF), 10.0-10,000.0 parts of hyaluronic acid, 0.003-3000.0 parts of phospholipid, 0.001-10.0 parts of metallothionein, 0.001-10.0 parts of collagen, 10.0-5000.0 parts of water-soluble pearl powder the weight percentage of which is 20%, and 0.001-200.0 parts of cholesterol. The sun-screening skin care product provided by the invention has the beneficial effects that the liposome is composed of phospholipid bilayers and contains pure water micro vesicles, and the vesicles can enclose a certain amount of nutrients and essences, are 100 nanometers in mean diameter which is 1 / 200-1 / 300 of the human cell diameter, can easily penetrate through the surface layer of human skin and can be fused with cells, thus exerting efficacy. The liposome sun-screening skin care product can deeply humidify horny cells and enhance hydration to improve the skin softness, and can provide sufficient nutrients to ensure skin health and increase skin resistance.

The invention relates to a follicular cell activator composition and preparation and their application. A follicular cell activator comprises at least two of a bFGF (basic fibroblast growth factor), an KGF or KGF1 (keratinocyte growth factor or keratinocyte growth factor 1), a KGF2 (keratinocyte growth factor 2), an IGF-1 (insulin-like growth factor 1), a VEGF (vascular endothelial growth factor),an HGF or HGF alpha (hepatocyte growth factor or hepatocyte growth factor alpha), an FGF18 (fibroblast growth factor 18), an SCF (stem cell factor), an FGF20 (fibroblast growth factor 20), an NGF (never growth factor), a GH (growth hormone) and Wnt3a protein. The follicular cell activator preparation herein can regulate proliferation, differentiation, regeneration and metabolism of follicular cells, and improve microcirculation to restore follicles and effectively treat seborrheic alopecia and other types of hair loss.

The invention discloses a skin anti-ageing skin composition. The skin anti-ageing skin composition comprises the following components: components A which comprise 0.50-10.0 parts by weight of collagen, 20-40 parts by weight of glycerinum, 3-8 parts by weight of hydrolyzed rice protein and 3-8 parts by weight of sweet almond seed extract, and components B which comprise (based on the weight of the components (A) 1000IU / g-50000IU / g of sodium hyaluronate and 100 IU / g-5000 IU / g of keratinocyte growth factor. The skin anti-ageing skin composition disclosed by the invention is free of side effect, can be used for effectively improving the couperose skin, repairing skin injury, promoting production of novel cells and resisting skin aging, and has obvious scar-removing and radiation-preventing effects, so that the skin is tender and perfect, and therefore, abnormal skin manifestations such as stains and hyperpigmentation can be eliminated; besides, the skin anti-ageing active composition can be independently used or added with other components for being processed into various skincare products or cosmetics.

The invention belongs to the technical field of daily care products and discloses a moisturizing and repairing composition and an epidermis biomimetic membrane liquid crystal complex constructed therefrom. The core components of the moisturizing and repairing composition and the epidermis biomimetic membrane liquid crystal complex include ceramide, phytosphingosine, cholesterol, lauryl glutamate, squalane, myristyl alcohol, cetostearyl alcohol, arginine, glycine and hydroxyproline. In the invention, by means of combination of various raw materials and through a layered liquid crystal forming mechanism, the special epidermis biomimetic membrane is developed, by which a lamellar liquid crystal body, which likes a keratinocyte intercellular lipid layer, can be formed, so that an effect of protecting skin and improving skin can be achieved.

The invention discloses a bionic camellia extract-containing composition for repairing skin barrier and application of the composition. The composition is prepared from skin surface lipid film repairing components: squalene substances, fatty acid, triglyceride and cholesterol analogues; keratinocyte interstitial components: free fatty acid, ceramide and cholesterol analogues; synergistic interaction componentcomprises: a relieving and inflammation diminishing component, an anti-aging component, a whitening component and a skin penetrating agent; wherein the skin surface lipid film repairing and keratinocyte interstitial components are prepared from the camellia extract, camelina oil, oat kernel oil, squalane, phytosterol and ceramide 3. According to the composition for repairing the skin barrier, on the basis of the principle of the human skin barrier, keratinocyte interstitial components and skin surface lipid film repairing components which are similar to the human skin barrier in structure are subjected to bionic matching through a large number of formula tests and studies, the components in the skin barrier are safely simulated, and the complete and healthy epidermal barrier isremodeled.

The production process of human horny cell growth factor-2 includes the steps of: culturing engineering colibacillus carrying expression vector selected from pSE280, PSE380 and pSE420 under the proper expression condition and inserting the encoding sequence of human horny cell growth factor in the polyclonal site of the said expression vector so as to express human horny cell growth factor; and separating and purifying the expressed human horny cell growth factor-2. The production process of the present invention has high expression amount and simple separation and purification steps. The present invention provides also corresponding expression vector and engineering bacterial.

The present invention relates to a pharmaceutical composition for cell therapy of pigmentation disorders, which comprises as an active ingredient (a) melanocytes and keratinocytes, or (b) melanocytes and fibroblasts. The cell therapy composition of this invention allows for the first time to treat pigmentation disorders through cell therapy regimen. The present composition comprises as active ingredients a mixed cell preparation of melanocytes and keratinocytes or a mixed cell preparation of melanocytes and fibroblasts such that it induces very effectively pigmentation to treat diseases associated with hypopig mentation such as vitiligo.

Resveratrol, a component found in common edible plants, including peanuts and red grapes, is a phytoestrogen. Resveratrol can inhibit the proliferation of skin epidermal cells (keratinocytes) and promote their differentiation. Resveratrol has also been found to relieve skin irritation caused by alpha hydroxy acids. Resveratrol is effective in improving the appearance of wrinkled, lined, dry, flaky, aged or photodamaged skin and improving skin thickness, elasticity, flexibility, radiance, radiance and fullness.

The invention provides a human keratinocyte growth factor -1 structure analogues KGF-1delta 23KGF(40S), an N end of an amino acid sequence of which lacks 23 amino acids, while the 40-bit cysteine point of which is mutated into a nonpolar amino acid. The invention also relates to a production method of the structure analogues, which carries out the fusion expression with a small ubiquitin related modifier gene mature peptide, while a fusion protein and the ubiquitin related modifier gene protease 1 co-express in the prokaryotes. In the process of fermentation expression, the ubiquitin related modifier gene protease 1 can hydrolyze the fusion protein to produce a soluble KGF-1delta 23KGF (40S). The human keratinocyte growth factor structure analogues can facilitate the proliferation of the keratinocyte cells, the growth of the hair follicle cells and inhibit the growth of the fibroblast cells, and has the functions of anti-scar, anti-fibrosis, epidermis healing facilitation and corneal wound reparation, etc.

The invention provides a method for preparing recombinant human keratinocyte growth factor-2 by secretion expression. The method provides an expression vector of the recombinant human keratinocyte growth factor-2, which contains an secretion signal peptide coding sequence and can secrete KGF-2 protein in periplasm for expression; and when a host cell containing the recombinant plasmid is subjected to repeated freeze thawing at a low temperature, the recombined protein can be released in a buffer solution under the condition of not damaging inner membranes of bacilli and the activity of the protein.

The invention discloses a preparation method of novel tissue engineering skin. The method includes steps of jointly culturing an amniotic membrane decellularized scaffold and a mesenchymal stem cell to be a carrier, inoculating horn cell; culturing by a serum-free culturing system, and acquiring a complete tissue engineering skin membrane. Through the method, the method can culture a complete skin membrane and the skin membrane can be applied to the skin scar repair and leucoderma treatment. The horn cell is originated from body skin or prepuce of the newborn. The carrier is low in immunogenicity while also has biological activity; the serum-free culturing system can realize the in-vitro serum-free cultivation and amplification of the horn cell, and improve cell growth, multiplication, cell form, activity and function instead of bad influence; the skin membrane cultivated by the method is approached to natural skin; after the skin-grafting culturing, it can be applied to the scar repair of scaled and other injured skins as well as treatment of skin disease such as leucoderma.

The invention provides a method for evaluating skin conditions by skin keratinocyte form. The method comprises the following steps of after a special skin keratinocyte patch is sampled, heating the patch by microwaves, dyeing by ferrous sulfate, redyeing by HE (hematoxylin-eosin), and observing the skin keratinocyte form under a microscope, including the polymerizing degree, arrangement type, area, health degree and melanin of the skin keratinocyte, so as to evaluate the skin conditions. The method has the advantages that the sampling is convenient, the observed picture is clear after dyeing,the operation can be completed by only using one patch, the efficiency is improved, and the cost is reduced.

The invention belongs to the technical field of cosmetics, and discloses a plant acne-removing compound as well as a preparation method and applications of the plant acne-removing compound. The plant acne-removing compound is prepared from the following components in parts by weight: 40-80 parts of shallot extracting solution, 10-20 parts of wild chrysanthemum flower extracting solution, 10-20 parts of lotus leaf extracting solution, and 2-10 parts of betaine salicylate. The shallot extracting solution can effectively inhibit the breeding of propionibacterium acnes and kill bacteria; the lotus leaf extracting solution can effectively control the secretion of skin sebum; the wild chrysanthemum flower extracting solution has the effects of clearing away heat and toxic materials, and diminishing inflammation; betaine salicylate can accelerate the falling of the human epidermal keratinocytes; through the synergistic effect of the four components, the plant acne-removing compound achieves the effect of inhibiting propionibacterium acnes from the formation mechanism of acnes.

The invention discloses essence oil capable of repairing damaged sebum film and damaged cuticle. The essence oil is prepared from the following raw materials in parts by weight of 0.1 to 20 parts of meadowfoam seed oil, 5 to 25 parts of squalane, 20 to 55 parts of macadamia integrifolia seed oil, 0.1 to 10 parts of tocopherol, 0.1 to 10 parts of sphingolipids, 0.1 to 10 parts of ceramide 2, 0.1 to 10 parts of ceramide 3, 0.1 to 10 parts of ceramide 4 and 0.1 to 10 parts of ceramide 6II. The essence oil disclosed by the invention can well simulate the structures of a human body sebum film and a cuticle to form a protective barrier covering the outermost layer of skin; And by matching with a ceramide compounding technology, a lipid part in keratinocytes in a cuticle can be well filled, so that the skin barrier is completely repaired, external invasion is resisted, the greasy feeling of most closed grease on the market is improved, the spreadability is better than that of common skin care oil, and the skin feeling is fresh and cool.

A method for preparing corneocites specimen, which comprises stripping off corneocites from the surface of a skin using an adhesive material, staining the corneocites in a solution of stain in solvent containing a water-miscible organic solvent, and mounting the stained corneocites into an oil and fat constituent and / or composition that is liquid at 1 atm, 25° C.

The invention discloses an essence cream capable of whitening the skin and removing wrinkles and a preparation method thereof. The essence cream is prepared by mixing a moisturizing agent, bioactive polypeptides, a nano silver sol dispersion solution, a pH regulator, a surfactant and deionized water according to a certain proportion. No organic preservative is added into the essence cream; nano silver is used as a preservative antibacterial agent; the essence cream has a preservative function and also has functions of resisting bacteria, diminishing inflammation and accelerating repair of damaged skin; the bioactive polypeptides are easily absorbed by the skin and are capable of accelerating the growth of epidermic cells, repairing epidermis, accelerating keratinocytes to secret fibrin andmaintaining cell elasticity; meanwhile, moisturizing components are added into the essence cream; the essence cream has a function of deeply nourishing the skin, effectively relieving dryness and itch of the skin, accelerating improvement of the skin structure from inside to outside, accelerating repair of the skin, improving the elasticity of the skin to a certain extent, enabling the skin to befiner, more smooth, more tender, whiter and extremely elastic and meeting the daily skincare requirements on whitening and wrinkle removal of people.

The invention discloses an externally applied medicament for treating skin diseases, which consists of the following Chinese herbs and chemical reagents: the weight proportion of the Chinese herbs and chemical reagents is 1:2 to 3; the Chinese herbs consist of sophora flavescens ait and smilax glabra and the weight proportion is sophora flavescens ait 1: smilax glabra 1 to 2; the chemical reagents consist of a salicylic acid, a benzoic acid and a boric acid and the weight proportion is salicylic acid 4 to 6: benzoic acid 3 to 5: boric acid 2 to 4. The medicament is characterized in that corneocyte is released by the salicylic acid and then fall off, thus leading bacteria and insects to have no hiding-places; dispelling wind and disinsection, heat-clearing and detoxifying and relieving itching are carried out by using Chinese herbs, and then fungus are killed and the growth is inhibited by the benzoic acid and the boric acid, reaching the purposes of completely clearing the fungus in the skin, no infection and recurrence. Compared with the present treatment medicaments, the external-use medicament has the advantages of convenient usage, short course, low treatment cost, obvious treatment effects, etc.