The invention discloses a preparation method of lacquer tree seed wax oil. The preparation method comprises the following steps: cooling lacquer tree seeds, rubbing the seeds, screening, crushing, pneumatically conveying and selecting, cooking, solvent extracting, filtering, separating and finally obtaining the lacquer tree seed wax oil. The preparation method of the lacquer tree seed wax oil can enable preparation, extraction and separation of the lacquer tree seed wax oil to be continuous, improves production efficiency, material utilization rate and wax quality, and improves wax market competitiveness. By applying the method, the husk-core separating efficiency of the lacquer tree seeds reaches over 95%, and the lacquer tree seed core perfectness ratio reaches over 90%; and moreover, compared with the existing separating method, the whole set of process flow is reliable in operation and strong in economical efficiency, so that processing cost for per tonnage of seeds is lowered by over 15%.