The present invention provides a method for constructing a characteristic spectrum of a
Glechoma galbana medicinal material, a standard decoction, or a
traditional Chinese medicine formula granule, comprising the following steps: preparing a
chlorogenic acid reference substance and a
chlorogenic acid reference substance solution; preparing a mixed reference substance solution and a
neochlorogenic acid and cryptochlorogenic acid reference substance solution; preparing a
test solution and subjecting the
chlorogenic acid reference substance solution, the mixed reference substance solution, and the
test solution to ultra-
high performance liquid chromatography analysis, respectively; wherein the ultra-
high performance liquid chromatography analysis conditions include: mobile phase A is
acetonitrile, mobile phase B is aqueous
phosphoric acid, and a
gradient elution procedure is employed. The characteristic spectrum constructed by the method described in the present invention is rich in information, highly characteristic, and has good reproducibility.