56 results about "Enzyme synthesis" patented technology

The invention discloses application of copper-amino acid nano enzyme in preparation of anti-inflammatory drugs, and relates to the technical field of biomedical new materials, amino acids comprise glycine, arginine, histidine, threonine, phenylalanine and cysteine; the ratio of the amino acid to the copper ions is 1: (0.5-5), and the synthesis temperature of the copper-cysteine nano enzyme is 25-125 DEG C. The copper-amino acid nano-enzyme library established by the invention has efficient hydroxyl free radical, superoxide free radical and hydrogen peroxide scavenging activity; wherein the copper-cysteine nano-enzyme shows the highest enzymatic activity, and shows low toxicity and good biocompatibility in both the cell level and the animal level; meanwhile, the copper-cysteine nano-enzyme also shows anti-inflammatory activity and anti-oxidative stress activity, can remarkably improve cell inflammation and body inflammation, relieves and treats dextran sodium sulfate induced mouse ulcerative colitis, and can be further applied to preparation of drugs for treating inflammatory bowel diseases.

The invention belongs to the technical field of biochemical engineering, and discloses a production method for improving the content of odd-chain fatty acid in grease by modifying schizochytrium limacinum and optimizing a substrate and application of the method. According to the method, schizochytrium limacinum HX-308 serves as a receptor strain, high-yield odd-chain fatty acid engineering bacteria are obtained through gene overexpression and inhibition, and the high-yield odd-chain fatty acid is obtained. Accumulation of a propionyl CoA precursor pool for synthesizing odd-chain fatty acid is improved, endogenous methylmalonyl-CoA mutase coding gene MUT is inhibited, so that a competitive pathway of the odd-chain fatty acid is inhibited, and then through substrate optimization, the content of fermented grease and the content of the odd-chain fatty acid are obviously improved. The expression of related enzymes in schizochytrium limacinum is adjusted through a genetic engineering strategy to improve the odd-chain fatty acid synthesis, the synthesis and utilization of the key intermediate propionyl coenzyme A in the odd-chain fatty acid synthesis process are increased, the metabolic pathway of the propionyl coenzyme A is inhibited, and substrate optimization is assisted to improve the odd-chain fatty acid yield.

The invention discloses a black fungus AMY protein and a coding gene and application thereof, and belongs to the technical field of protein engineering. The invention provides a black fungus AMY protein as well as an encoding gene and application thereof in order to excavate a key enzyme gene of a starch sucrose metabolic pathway in black fungus, and discovers and verifies that the black fungus AMY protein and the encoding gene thereof play a key regulation and control role in inducing synthesis of black fungus alpha-amylase through transcriptome sequencing. A solid foundation is laid for deeply exploring the function of a key gene of a starch sucrose metabolic pathway.

The application discloses a kind of high-activity polyphenol oxidase and preparation method thereof, belong to nano mimic enzyme synthesis technical field.The polyphenol oxidase of the application is with copper ion as active center, 2-methyl imidazole and guanylate disodium as organic ligand.Preparation method thereof includes the following steps: guanylate disodium solution is added to copper chloride solution under stirring condition, to obtain first mixed solution;First mixed solution is slowly added to 2-methyl imidazole solution under stirring condition, to obtain second mixed solution;Second mixed solution is centrifuged after ultrasonic at room temperature, to obtain brown precipitate, and the polyphenol oxidase is prepared by washing and drying brown precipitate.The mimic enzyme prepared by the application has higher catalytic performance, better stability and feasibility of large-scale preparation compared with natural enzyme, and will have broad application prospect in the field of biosensor.

The invention relates to a chemoenzymatic synthesis method of a high-glucose-type homogeneity mucoprotein core structural domain. The method comprises the following steps: firstly, catalytically synthesizing a key alpha-linked glycosylated amino acid block Fmoc-GalNacalpha-Ser / Thr-OH at a low temperature (-20 DEG C to 0 DEG C); then assembling the building blocks on a repeated polypeptide skeleton rich in proline, serine and threonine at accurate intervals (3-5 amino acid residues) through a solid-phase peptide synthesis technology to form a'sugar chain growth scaffold '; then, a series of high-specificity glycosyl transferases are used for carrying out sequential and directional sugar chain enzymatic extension on the scaffold, and a uniform core structure (such as a core type 3) is constructed; and finally, performing high-efficiency purification by a three-step series method of size exclusion chromatography, ion exchange chromatography and lectin affinity chromatography. The technical bottleneck of inhomogeneity (lt, 70%) of glycoforms in a traditional method is overcome, the mucoprotein core fragment with glycoform homogeneity larger than or equal to 95% and bioactivity highly similar to that of natural mucoprotein can be prepared on a large scale, and the method has wide application prospects in the biomedical fields of mucous membrane protective agents, drug delivery carriers and the like.

The invention provides non-naturally occurring microbial organisms containing enzymatic pathways and / or metabolic modifications for enhancing carbon flux through acetyl-CoA. In some embodiments, the microbial organisms of the invention having such pathways also include pathways for generating reducing equivalents, formaldehyde fixation and / or formate assimilation. The enhanced carbon flux through acetyl-CoA, in combination with pathways for generating reducing equivalents, formaldehyde fixation and / or formate assimilation can, in some embodiments, be used for production of a bioderived compound. Accordingly, in some embodiments, the microbial organisms of the invention can include a pathway capable of producing a bioderived compound of the invention. The invention still further provides a bioderived compound produced by a microbial organism of the invention, culture medium having the bioderived compound of the invention, compositions having the bioderived compound of the invention, a biobased product comprising the bioderived compound of the invention, and a process for producing a bioderived compound of the invention.

The invention provides a synergistic treatment pharmaceutical composition for preventing and treating recurrence after hepatocellular carcinoma thermal ablation and application of the synergistic treatment pharmaceutical composition. Aiming at malignant tumor progression driven by ACSS3 gene silencing after thermal ablation, an epigenetic editing technology is innovatively combined with metabolite supplementation, and a preparation for specifically activating or promoting ACSS3 gene expression and sodium propionate are cooperatively used, so that ACSS3 can be remarkably reactivated or expression can be promoted in a xenograft model derived from a cell line and a patient; the compound can be used for recovering propionyl-coenzyme A synthesis and normal propionic acid metabolism and inhibiting fatty acid beta-oxidation, so that the growth and metastasis of post-ablation hepatocellular carcinoma (HCC) are effectively blocked, and the limitation of a single therapy is overcome. A clearer mechanism target is provided for preventing recurrence after HCC thermal ablation, meanwhile, the prepared targeting nano-liposome preparation has a synergistic effect, and as a brand-new treatment strategy, the defect that intervention on recurrence roots is insufficient in the prior art is overcome.

This invention discloses a method and system for controlling the preparation of low-temperature, high-efficiency straw-promoting microbial agents, relating to the field of microbial agent preparation process control technology. It utilizes multiple types of sensors to collect a microbial agent preparation dataset RAW(t) at time t, calculates and constructs a metabolic feature vector Vm(t) at time t, achieving real-time quantification of the macroscopic metabolic activity of microorganisms. Furthermore, it calculates the respiratory entropy Q at time t. R (t) and calculate the enzyme synthesis potential E at time t. S (t) and construct the diagnostic state vector Ds(t) at time t, thereby transforming external measurable parameters into a deep insight into the internal production potential of the microbial cells, objectively and dynamically diagnosing the fermentation process into different physiological stages, and generating a stage identifier ID(t) at time t, automatically executing the matching fine control strategy AIR, ensuring that optimal regulation is automatically adopted at each stage, thereby significantly improving the quality, production efficiency and batch stability of the microbial agent product.

The invention discloses a plant source leuco-anthocyanidin dioxygenase mutant and application thereof in synthesis of chiral drug intermediates, and belongs to the technical field of enzymology engineering. According to the invention, leuco-anthocyanidin dioxygenase (LDOX) derived from arabidopsis thaliana is modified through site-specific mutagenesis, an F304L / T239S double mutant (LDOXLS) is obtained, the (R)-phenylglycine analogue can be synthesized with the yield of 1.69 g / L, the yield of 81% and the enantiomeric excess ratio of 97: 3, and different substituent derivatives of the (R)-phenylglycine analogue can be generated. Compared with the traditional method, the method for synthesizing the (R)-phenylglycine analogue and the derivative thereof by using the enzyme has the advantages of wider substrate range, higher stereoselectivity, more environment-friendly requirement, simplicity and convenience in operation and the like.

The invention relates to the technical field of gene engineering, in particular to high-activity peptide ligase and application thereof in synthesis of tilpotide. The high-activity peptide ligase provided by the invention shows catalytic efficiency far higher than that of an existing enzyme in a specific ligation reaction aiming at an acyl donor and an acyl receptor of a precursor fragment of the tilpoitide; by systematically transforming a host strain, the high-activity peptide ligase which can be secreted and expressed at high level and high fidelity and has correct folding and complete activity is obtained, and meanwhile, the degradation of a product is reduced to the maximum extent; and an optimal expression element combination of the high-activity peptide ligase is obtained through high-throughput optimization of an expression system. The method is finally integrated into a complete new process for synthesizing the tilpoitide by the chemical enzyme method, which can realize kilogram-level production, and is suitable for popularization and application.

The invention discloses an enzymatic synthesis method of a melogabalin intermediate and related alcohol dehydrogenase, racemic 3-ethyl bicyclo [3.2. 0] hept-3-ene-6-ketone is used as a substrate, a genetically engineered bacterium for producing alcohol dehydrogenase is used as an enzyme catalyst, a genetically engineered bacterium for producing formate dehydrogenase, ammonium formate and coenzyme are matched, an enzymatic reaction is carried out under a stirring condition, and the melogabalin intermediate is obtained. And carrying out purification and separation on a reaction product to obtain the melogabalin intermediate (1R, 5S)-3-ethyl bicyclo [3.2. 0] hept-3-ene-6-ketone. The method has the advantages of simple reaction, few steps, mild reaction, less pollution and the like, and has great industrial application potential and commercial value.

The invention discloses serratia marcescens for producing terpenoids and application of the serratia marcescens, and belongs to the technical field of bioengineering. According to the invention, part of genes, such as acetoacetyl-coenzyme A thiolase, 3-hydroxy-3-methylglutaryl-coenzyme A synthetase, 3-hydroxy-3-methylglutaryl-coenzyme A reductase and phosphomevalonate decarboxylase, in a haloarchaea MVA pathway are integrated in chromosomes of a serratia marcescens host, and mevalonate kinase is expressed on plasmids, so that the recombinant serratia marcescens is obtained. Meanwhile, competitive genes are knocked out, and a universal terpenoid production strain is constructed. Synthetases of other terpenoids are further introduced into the universal terpenoid production strain and are used for synthesizing more terpenoids.

Disclosed herein are polynucleotides comprising sequences encoding coenzyme M synthase (ComF) linked to a heterologous regulatory element and methods of using the same. The polynucleotides may comprise synthetic operons comprising additional sequences encoding enzymes, e.g., a taurine-pyruvate aminotransferase, a sulfoacetaldehyde acetyl transferase, or a sulfopyruvate decarboxylase. Also disclosed herein are recombinant prokaryotic cells, e.g., recombinant bacterial, e.g., E. coli, or archaeal cells, e.g., Methanosarcina acetivorans with improved tolerance to oxidative stress.

The utility model belongs to capric acid glyceride production equipment technical field, concretely relates to a kind of enzyme synthesis capric acid glyceride reaction kettle, including reaction kettle, agitator, it is characterized by: reaction kettle top is equipped with the quick sampling mechanism installed by tank top flange one, tank top flange two, quick sampling mechanism includes the pipeline system of venturi, suction tube, connecting pipe, wherein the top end of suction tube is connected to venturi throat, the bottom end of suction tube is inserted into the inside of reaction kettle, venturi two ends are connected nitrogen valve and backwash valve respectively, backwash valve connects reaction kettle inside, connecting pipe connects sample release pipe, sample release valve is installed on sample release pipe, nitrogen valve is connected with nitrogen pipeline.The flow of nitrogen in venturi safely and conveniently takes out reaction liquid, effectively improves sampling convenience and safety;Reaction liquid can be circulated in quick sampling mechanism and reaction kettle to avoid sample cross-contamination.

The invention firstly discloses a method for synthesizing the spraadine A and the 3-OH spraadine A by utilizing a chemical-enzyme method. The synthetic route mainly comprises three steps: 1) synthesizing (-)-beta-CPA through a novel chemical enzyme method synthetic route; (2) catalyzing the (-)-beta-CPA in vitro by using oxidation cycloaddition enzyme CpaO to carry out intramolecular asymmetric oxidation [3 + 2] cycloaddition reaction to generate (-)-alpha-CPA; and (3) by taking the (-)-alpha-CPA as a substrate, synthesizing alpha-CPA natural products in a high oxidation state, such as speradine A, 3-OH speradine A and the like, through chemical modification. The invention has important theoretical and practical significance for three-dimensionally and specifically preparing or synthesizing a series of high-oxidation-state alpha-CPA natural products, and provides a leading compound for the development of anti-cancer drugs.

The present invention relates to a recombinant microorganism for producing 1,3-propanediol and a method for producing 1,3-propanediol using same. More specifically, the present invention provides a recombinant microorganism having an improved ability to produce 1,3-propanediol through overexpression of a transaldolase-encoding gene (tal), a succinyl-CoA synthetase-encoding gene (sucCD), a cystathionine γ-synthase-encoding gene (metB), an aspartate ammonia lyase-encoding gene(aspA), an aspartate aminotransferase-encoding gene (aspB), or a functional fragment thereof, and a method for producing 1,3-propanediol using same.

The present application relates to the technical field of enzyme synthesis of target compounds, and particularly relates to a method for preparing diglyceride vegetable oil by enzyme method, which comprises the following steps: mixing vegetable oil and glycerol according to a mass ratio of 3:1, then adding 5%-10% of a composite enzyme resin to the mixed liquid in terms of the mass of the vegetable oil, oscillating and treating at 40-60 DEG C for 8-16h, and then performing solid-liquid separation to obtain the diglyceride vegetable oil; wherein the composite enzyme resin takes macroporous resin AB-8 as a carrier, the carrier pores are fixed with partial glyceride lipase, the carrier exterior is coated with a gel layer, and the gel layer is dispersed with Candida antarctica lipase A. The method for preparing the diglyceride vegetable oil by enzyme method provided by the present application shortens the original method for obtaining the diglyceride vegetable oil crude product in steps to one step, belongs to a one-pot operation, and the diglyceride content of the diglyceride vegetable oil produced finally is high.

The invention relates to the technical field of cyclopeptide drug synthesis, in particular to a method for synthesizing mirror cyclopeptide by using OaAEP1 enzyme under the assistance of thioester, which comprises the following steps: firstly, synthesizing a linear mirror cyclopeptide thioester precursor by using an N-fluorenylmethoxycarbonyl (Fmoc) solid-phase polypeptide synthesis method, and then generating the mirror cyclopeptide from the thioester precursor under the mediation of the OaAEP1 enzyme. According to the invention, the mirror image ring melittin (D-cMelittin) is synthesized by using OaAEP1 enzyme under the assistance of thioester for the first time, and the mirror image ring melittin (D-cMelittin) has high protease stability and good antibacterial activity.

The invention relates to the technical field of enzyme catalysis, and particularly discloses a method for biologically synthesizing succinic acid in vitro by taking L-glutamic acid as a raw material, which constructs a new path for synthesizing L-glutamic acid-3-cyanopropionic acid-succinic acid by a full-biological enzyme method for the first time. A green conversion chain from cheap and bulk amino acids to a high value-added biodegradable plastic precursor is broken through, an L-glutamic acid resource with leading productivity is in butt joint with a poly (butylene succinate) (PBS) industry chain in shortage in the market, and the whole process of finally synthesizing succinic acid from L-glutamic acid through a 3-cyanopropionic acid intermediate is realized. According to the process, full-water-phase and extremely-mild one-pot method or multi-enzyme coupling conversion is realized. The two-step reaction is carried out in a nearly neutral water phase system, and the compatibility of enzyme is excellent, so that the process flow is greatly simplified, the equipment investment and energy consumption are reduced, common harmful byproducts in chemical synthesis are reduced from the source, and the biological safety and extremely high purity of the product succinic acid are ensured.

The application provides a genetically engineered bacterium with high yield of dihydroquercetin, a preparation method and application thereof, relates to the technical field of genetic engineering, and comprises a malonyl coenzyme A synthesis module, a tyrosine deaminase expression module, a naringenin synthesis module and a dihydroquercetin post-modification module. The enzymatic property of the fusion protein is increased by about 50% of the enzyme activity before fusion, the activity of converting naringenin into dihydroquercetin is greatly improved, and finally the yield of dihydroquercetin expressed in cyanobacteria is greatly improved. Meanwhile, BauA, MCR-C, Sam8, 4CL, CHS, CHI and expression fusion protein N33'H are expressed in cyanobacteria. The yield of dihydroquercetin is greatly increased under seawater culture conditions. The technical problem that the yield of biosynthetic dihydroquercetin is low and cannot meet the requirements of industrial application in the prior art is solved.

The invention discloses a pesticide synergist containing platinum nano enzyme and a preparation method of the pesticide synergist. The pesticide synergist comprises nano silicon dioxide, the platinum nano enzyme, chitosan and a surfactant. The preparation method comprises the following steps: S1, synthesizing hollow dendritic nano silicon dioxide; s2, synthesizing platinum nano enzyme; and S3, synthesizing the pesticide synergist containing the platinum nano enzyme. According to the invention, the preparation conditions of the platinum nano enzyme are optimized, and the production requirements are reduced. The nano-enzyme agricultural synergist prepared by the method disclosed by the invention can be used for flight control, and the labor cost is reduced.

The present application relates to the technical field of cyclic peptide drug synthesis, and particularly relates to a method for synthesizing mirror image cyclic peptide by using OaAEP1 enzyme with the assistance of thioester, which comprises the following steps: firstly, a linear thioester precursor of mirror image cyclic peptide is synthesized by using an N-fluorenylmethyloxy carbonyl (Fmoc) solid-phase polypeptide synthesis method; and then the thioester precursor can generate the mirror image cyclic peptide under the mediation of OaAEP1 enzyme. The present application first synthesizes mirror image melittin (D-cMelittin) by using OaAEP1 enzyme with the assistance of thioester, and the mirror image melittin has high protease stability and good antibacterial activity.

This invention relates to a recombinant bacterial cell for de novo synthesis of xanthocyanin WGX-50 using glucose as a carbon source, its construction method, and its applications. The recombinant bacterial cell uses *Escherichia coli* as the host strain and integrates the following three heterologous biosynthetic modules: a 3,4-dimethoxyphenylethylamine de novo synthesis module, which contains an enzyme system catalyzing the generation of dopamine from L-tyrosine and further stepwise methylation of dopamine to 3,4-dimethoxyphenylethylamine; a cinnamoyl-CoA synthesis module, which contains an enzyme system catalyzing the generation of cinnamoyl-CoA from phenylalanine or glucose metabolic intermediates; and an amidation module, which contains an acyltransferase capable of catalyzing the condensation of 3,4-dimethoxyphenylethylamine and cinnamoyl-CoA to generate xanthocyanin WGX-50. This invention achieves, for the first time, the de novo biosynthesis of xanthocyanin in microorganisms, completely eliminating dependence on the expensive exogenous precursor 3,4-dimethoxyphenylethylamine and significantly reducing raw material costs.

The invention provides a biosynthesis method for preparing urolithin A. According to the biosynthesis method for preparing urolithin A, through four-step reaction, whole-enzyme synthesis of urolithin A is achieved. According to the invention, the invention also provides a whole enzyme method process for preparing urolithin A on a small scale. Compared with a chemical synthesis method or a microbial fermentation method, the whole enzyme method shows advantages in multiple aspects.

The invention relates to an esterase pNBE mutant and application of a recombinant plasmid of the esterase pNBE mutant in synthesis of cephalosporin drug intermediates, and belongs to the technical field of enzyme synthesis and catalysis. In order to solve the problem that the existing esterase has low enzyme esterlysis activity on RC, the invention provides an esterase pNBE mutant and an application of a recombinant plasmid thereof in synthesis of a cephalosporin drug intermediate, the amino acid sequence of the esterase pNBE mutant is formed by mutation of an amino acid sequence as shown in SEQ ID NO.1, and the recombinant plasmid containing the esterase pNBE mutant. Under the catalysis of an esterase pNBE mutant, a substrate RC is subjected to an enzyme esterlysis reaction, and a product 7-ACCA is generated. The esterase pNBE mutant disclosed by the invention can be used for improving the enzymatic esterlysis capability of the esterase pNBE mutant on RC, has high enzymatic activity, can be used for removing carboxyl protecting groups in RC, can be fully converted into 7-ACCA products, and has the advantages of high yield and good purity and quality.

The invention discloses a chemical enzyme synthesis method of chiral aporphine alkaloids, and relates to the field of biochemistry, the method comprises the following steps: taking a phenylethylamine compound and a phenylacetic acid compound as initial raw materials, and carrying out amide condensation and dehydration cyclization to form a compound IV; then reducing through imine reductase or an imine reductase mutant to form a V-type compound; then, a compound VI is formed through a methylation reaction of methyltransferase or Eschweiler-Clarke or methyl iodide, and the compound VI is formed through a methylation reaction of methyltransferase or Eschweiler-Clarke or methyl iodide; and finally, carrying out photocatalytic coupling to obtain the aporphine alkaloid. According to the preparation method disclosed by the invention, purification is not needed in the preparation reaction process from the IV-type compound to the VI-type compound, and the compound can be directly used for the last step of photocatalytic coupling; the optically pure (S)-VII aporphine alkaloid can be obtained; the method is mild in reaction condition, green, environment-friendly, simple in process, low in cost, capable of reaching gram-level scale and suitable for industrial production.

The invention relates to a preparation method of citryl coenzyme A, and belongs to the technical field of biology. The method comprises the following steps: taking citric acid, gastrodin and acetyl coenzyme A as raw materials, and generating citryl coenzyme A under the catalysis of citryl coenzyme A synthetase A obtained by CCSA region coding and citryl coenzyme A synthetase B obtained by CCSB region coding; as the citryl coenzyme A as an intermediate is difficult to separate and does not have a standard substance, the citryl coenzyme A finally passes synthesis indirect verification of a final product parishin C. The parishin C is synthesized through heterologous biology, the controllability is high, the requirement for raw material planting can be reduced, the yield of produced products is high, and the problems of environmental pollution and the like caused by chemical synthesis can be solved. The citryl-coenzyme A synthetase gene is used as a key gene for synthesizing an intermediate of parishin C, assists the synthesis of the parishin C, and can be used for the breeding research of the parishin C.

The application discloses a genetic element for enhancing lactic acid metabolic flux of actinomycetes, and belongs to the field of synthetic biology and microbial metabolic engineering. sucC The application successfully constructs a single expression element and a co-expression element by cloning genes from Streptomyces coelicolor sucD , and verifies that the SucC protein and the SucD protein both have lactic acid coenzyme A synthetase activity through in-vitro protein purification and enzyme activity determination, and the enzyme activity of a protein complex (SucC-SucD, SucCD) expressed by the co-expression element is significantly higher than that of the single protein, indicating that the two proteins have a synergistic effect; the genetic element can efficiently catalyze the combination reaction of lactic acid and coenzyme A in vitro, accelerates the lactic acid metabolic speed, provides a core genetic element for the modification of the lactic acid metabolic pathway of actinomycetes, enriches a synthetic biology tool library, and has important industrial application prospects and academic value.

The present application belongs to the technical field of product separation of enzyme synthesis of antibiotics, and particularly relates to a method for separating amoxicillin and phenylacetic acid from a reaction solution for preparing amoxicillin in one step. In the present application, immobilized penicillin acylase mutants are used to catalyze the one-step synthesis of amoxicillin from penicillin potassium, and a set of methods for separating amoxicillin and phenylacetic acid from the obtained reaction mixture is developed. The technical scheme mainly comprises the following steps: firstly, the immobilized penicillin acylase mutants are separated from the reaction solution by filtration, and then amoxicillin is separated by crystallization; and then phenylacetic acid is separated and recovered by toluene extraction, back extraction and the like. The separation method can quickly and efficiently separate amoxicillin, and the yield of amoxicillin is high, with an average crystallization rate of 93.22%; at the same time, the separation and recovery effect of phenylacetic acid is good; and the extraction agent toluene can be recycled.

The invention relates to a gastrodia elata acyltransferase GeBAHD013 gene and application of the gastrodia elata acyltransferase GeBAHD013 gene in preparation of parishin C, and belongs to the technical field of biology. The nucleotide sequence of the gastrodia elata acyltransferase GeBAHD013 gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein of the gastrodia elata acyltransferase GeBAHD013 gene is shown as SEQ ID NO. 2. Gastrodin, citric acid and acetyl coenzyme A are used as raw materials, and under the catalysis of gastrodia elata acyltransferase obtained by gastrodia elata acyltransferase GeBAHD013 gene coding and citryl coenzyme A synthetase, parishin C is generated through a one-pot method. The parishin C is synthesized through heterologous biology, the controllability is high, the requirement for raw material planting can be reduced, the yield of produced products is high, and the problems of environmental pollution and the like caused by chemical synthesis can be solved. The gastrodia elata acyltransferase GeBAHD013 gene is used as a key gene for biosynthesis of the parishin C, and can be used for breeding research of the parishin C.