131 results about "Enzyme synthesis" patented technology

The invention relates to a T7 RNA polymerase mutant and application thereof. Specifically, the invention discloses a T7 RNA polymerase mutant, and the polymerase mutant has at least one amino acid mutation at sites corresponding to a wild type T7 RNA polymerase and selected from the following groups: the 44th site, the 45th site, the 46th site, the 172nd site, the 389th site, the 425th site, the 441st site, the 632th site, the 643th site, the 754th site and the 789th site; the serial number of the mutation site is based on the sequence shown in SEQ ID NO: 1, the mutation leads to obvious reduction of a byproduct dsRNA generated in the process of enzyme synthesis of mRNA, and meanwhile, the generation of RNA aggregates is reduced.

The invention discloses a recombinant strain for producing squalene as well as a construction method and application of the recombinant strain. The construction method comprises the following steps: heterologously expressing cDNA (complementary deoxyribonucleic acid) of each gene in a squalene synthesis route from filamentous fungi in yeast; the squalene synthetic pathway genes comprise a mevalonic acid-5-phosphate kinase gene, a squalene synthase gene, an acetoacetyl coenzyme A thiolase gene, a mevalonic acid kinase gene, a hydroxymethyl glutaryl coenzyme a synthetase gene, a mevalonic acid pyrophosphate decarboxylase gene, a farnesyl pyrophosphate synthetase gene and isopentenyl pyrophosphate. A dimethallylpyrophosphate isomerase gene and a truncated hydroxymethyl glutaryl coenzyme a reductase gene; the culture medium is characterized in that the filamentous fungi are Saroocladium oryzae or Stibella sp. 1056, and the culture medium is a culture medium. The excavated squalene synthesis route can complete efficient de novo synthesis of squalene, and the squalene yield is increased.

The invention discloses construction and application of a saccharomyces cerevisiae engineering strain for synthesizing vanillin by taking ferulic acid as a substrate, and belongs to the technical field of genetic engineering. The invention provides a recombinant saccharomyces cerevisiae, which is characterized in that a series of genes for producing vanillic acid and vanillyl alcohol from vanillin through oxidation and reduction are knocked out, and feruloyl coenzyme A synthetase FCS and p-hydroxycinnamyl coenzyme A hydratase ECH which are obtained by screening from a metagene library in a West Sichuan hot spring extreme environment are overexpressed. According to the engineering strain VAN002 with high yield of vanillin, the accumulation of vanillin reaches 16.8 g / L, and the molar conversion rate of catalyzing ferulic acid to synthesize vanillin reaches 93%, which is the highest level reported by synthesizing vanillin by saccharomyces cerevisiae at present.

The invention discloses a multifunctional nano-enzyme as well as a preparation method and application thereof, and belongs to the technical field of nano-enzyme synthesis. Aiming at the problems that a traditional nano-enzyme is single in activity, limited in treatment effect, poor in tumor active targeting specificity and the like, the multifunctional nano-enzyme provided by the invention is simple and efficient in preparation method, and is prepared by taking N and P double-doped hollow carbon nanospheres as a carrier, loading L-arginine and glucose oxidase and taking an aptamer as a targeting molecule. The multifunctional nano-enzyme is small in particle size, high in dispersion degree and good in biological safety. The multifunctional nano-enzyme not only has the activities of peroxidase, nitric oxide synthase and glucose oxidase, but also has excellent photo-thermal conversion capacity in tumor targeted therapy. The multifunctional nano-enzyme provided by the invention has a tumor focus specific recognition capability, can be highly enriched at a tumor part, finally realizes the purpose of photothermal / catalytic treatment combined tumor treatment, and provides a new thought and platform for tumor treatment.

The invention discloses application of copper-amino acid nano enzyme in preparation of anti-inflammatory drugs, and relates to the technical field of biomedical new materials, amino acids comprise glycine, arginine, histidine, threonine, phenylalanine and cysteine; the ratio of the amino acid to the copper ions is 1: (0.5-5), and the synthesis temperature of the copper-cysteine nano enzyme is 25-125 DEG C. The copper-amino acid nano-enzyme library established by the invention has efficient hydroxyl free radical, superoxide free radical and hydrogen peroxide scavenging activity; wherein the copper-cysteine nano-enzyme shows the highest enzymatic activity, and shows low toxicity and good biocompatibility in both the cell level and the animal level; meanwhile, the copper-cysteine nano-enzyme also shows anti-inflammatory activity and anti-oxidative stress activity, can remarkably improve cell inflammation and body inflammation, relieves and treats dextran sodium sulfate induced mouse ulcerative colitis, and can be further applied to preparation of drugs for treating inflammatory bowel diseases.

The invention belongs to the technical field of biochemical engineering, and discloses a production method for improving the content of odd-chain fatty acid in grease by modifying schizochytrium limacinum and optimizing a substrate and application of the method. According to the method, schizochytrium limacinum HX-308 serves as a receptor strain, high-yield odd-chain fatty acid engineering bacteria are obtained through gene overexpression and inhibition, and the high-yield odd-chain fatty acid is obtained. Accumulation of a propionyl CoA precursor pool for synthesizing odd-chain fatty acid is improved, endogenous methylmalonyl-CoA mutase coding gene MUT is inhibited, so that a competitive pathway of the odd-chain fatty acid is inhibited, and then through substrate optimization, the content of fermented grease and the content of the odd-chain fatty acid are obviously improved. The expression of related enzymes in schizochytrium limacinum is adjusted through a genetic engineering strategy to improve the odd-chain fatty acid synthesis, the synthesis and utilization of the key intermediate propionyl coenzyme A in the odd-chain fatty acid synthesis process are increased, the metabolic pathway of the propionyl coenzyme A is inhibited, and substrate optimization is assisted to improve the odd-chain fatty acid yield.

The invention discloses an amino acid sequence and application of a nucleotide sequence in synthesis of anisodine, and relates to the technical field of biology, the amino acid sequence is shown as SEQ ID NO.2, or the sequence shown as SEQ ID NO.2 is subjected to substitution, mutation or deletion of a plurality of amino acid residues, and the amino acid sequence with the same coding function is used for coding anisodine synthetase. The enzyme is used for catalytic synthesis of anisodine. The nucleotide sequence is as shown in SEQ ID NO.1, or the sequence as shown in SEQ ID NO.1 is substituted, mutated or deleted by a plurality of nucleotides, and the nucleotide sequence with the same coding function is used for coding the anisodine synthetase. An amino acid sequence for synthesizing the anisodine synthetase is found for the first time, the anisodine synthesis function of the anisodine synthetase is verified through a tobacco transient expression experiment, and the function of the anisodine synthetase is further verified through prokaryotic expression of in-vitro enzyme activity.

The present disclosure relates to methods of using single-stranded RNA ligase for synthesis of oligonucleotides, including oligonucleotides containing a conjugate moiety. In some embodiments, the present disclosure further provides recombinant single-stranded RNA ligases, polynucleotides encoding the recombinant single-stranded RNA ligases, and compositions of the single-stranded RNA ligases.

The invention belongs to the technical field of bioengineering, and relates to an ArsL mutant and an application of the ArsL mutant in synthesis of Arsinothricin. The ArsL mutant is a mutant obtained by mutating the 428th cysteine C of the amino acid sequence of wild AST synthetase ArsL into alanine A, and the amino acid sequence of the ArsL mutant is as shown in SEQ ID No.2. The invention also discloses a preparation method of the ArsL mutant. The invention also further provides an application of the ArsL mutant in catalysis of sodium methyl arsenate and S-adenosylmethionine to synthesize Arsinothricin. The invention further provides an application of the ArsL mutant in catalysis of sodium methyl arsenate and S-adenosylmethionine to synthesize Arsinothricin. The ArsL mutant disclosed by the invention has higher catalytic activity and thermal stability, the process of converting high-concentration MMA into AST is realized by utilizing the ArsL mutant, and the catalytic activity of the ArsLC428A mutant is obviously improved compared with that of wild type AST synthetase. In addition, compared with an organic synthesis path, a semi-organic enzyme synthesis path and a natural biological synthesis path, the yield of the synthesis path provided by the invention is obviously improved, and the ArsL mutant can be used as a potential industrial application enzyme and has a wide application prospect.

The invention discloses a synthesis method of an amino-acid-based MOFs (Metal-Organic Frameworks) derived nano-enzyme, and relates to the technical field of nano-enzyme synthesis, the synthesis method of the amino-acid-based MOFs derived nano-enzyme comprises the following steps: step 1, selecting proper metal ions and organic ligands; step 2, ultrasonic dispersion; step 3, precipitate drying; and step 4, activating treatment of the nano enzyme. The MOFs-derived nano-enzyme with alkaline phosphatase activity is synthesized by taking natural amino acid as a ligand and one of ions of zirconium, copper and iron as a metal node and introducing defects by a method of adding an acidic regulator in synthesis, and the crystal structure of the nano-enzyme is not changed under the introduction of the defects, so that the alkaline phosphatase activity of the MOFs-derived nano-enzyme is improved. The MOFs-derived nano-enzyme has the advantages that the MOFs-derived nano-enzyme is modified, the catalytic hydrolysis activity of the MOFs-derived nano-enzyme on phosphate substrates is greatly improved, good substrate specificity is shown, the structure of the MOFs-derived nano-enzyme does not change after long-term incubation under acidic, alkaline and high-temperature conditions, and compared with natural enzyme, the MOFs-derived nano-enzyme has good stability, pH stability and reusability.

The invention discloses a black fungus AMY protein and a coding gene and application thereof, and belongs to the technical field of protein engineering. The invention provides a black fungus AMY protein as well as an encoding gene and application thereof in order to excavate a key enzyme gene of a starch sucrose metabolic pathway in black fungus, and discovers and verifies that the black fungus AMY protein and the encoding gene thereof play a key regulation and control role in inducing synthesis of black fungus alpha-amylase through transcriptome sequencing. A solid foundation is laid for deeply exploring the function of a key gene of a starch sucrose metabolic pathway.

The application discloses a kind of high-activity polyphenol oxidase and preparation method thereof, belong to nano mimic enzyme synthesis technical field.The polyphenol oxidase of the application is with copper ion as active center, 2-methyl imidazole and guanylate disodium as organic ligand.Preparation method thereof includes the following steps: guanylate disodium solution is added to copper chloride solution under stirring condition, to obtain first mixed solution;First mixed solution is slowly added to 2-methyl imidazole solution under stirring condition, to obtain second mixed solution;Second mixed solution is centrifuged after ultrasonic at room temperature, to obtain brown precipitate, and the polyphenol oxidase is prepared by washing and drying brown precipitate.The mimic enzyme prepared by the application has higher catalytic performance, better stability and feasibility of large-scale preparation compared with natural enzyme, and will have broad application prospect in the field of biosensor.

The invention relates to the technical field of enzyme synthesis, and discloses a method for synthesizing coenzyme A by combining magnetic immobilized enzyme with weak electric field, comprising the following steps: S1, bacterial seed culture; S2, fermentation culture; S3, bacterial collection and preservation; S4, preparation of magnetic immobilized carrier and immobilization of intracellular enzyme protein of Brevibacterium ammoniagenes; S5, synthesis of coenzyme A by magnetic immobilized coenzyme A synthetase. Coenzyme A is synthesized by combining magnetic immobilized enzyme with weak electric field, with high unit titer, short synthesis time, reduced oxidation of reduced coenzyme A, and no need to add mercaptoethanol for reduction in the later stage. Brevibacterium ammoniagenes is broken to free several enzyme proteins required for coenzyme A synthesis, and the purified coenzyme A synthetase is immobilized by using activated hydroxy resin carrier, with mild immobilization conditions, high protein fixation rate, less enzyme inactivation, good stability of magnetic immobilized enzyme, and one-time catalysis of pantothenic acid, adenosine triphosphate and cysteine ​​to synthesize coenzyme A at a fast speed.

The invention provides a metabolic engineering transformation method of high-yield coenzyme Q10 of rhodobacter sphaeroides and application of transformed engineering bacteria. The method comprises the following steps: knocking out geranyl and geranyl pyrophosphate synthase gene crtE participating in a carotenoid synthesis route; performing overexpression on a farnesyl pyrophosphate synthase gene ispA; a histidine kinase coding gene prrB is mutated, and the mutant is introduced. According to the invention, a combined regulation strategy suitable for targeted regulation is found, the metabolic flux synthesized by the coenzyme Q10 is accurately optimized, and the capability of producing the coenzyme Q10 by the rhodobacter sphaeroides is greatly improved.

The invention relates to a chemoenzymatic synthesis method of a high-glucose-type homogeneity mucoprotein core structural domain. The method comprises the following steps: firstly, catalytically synthesizing a key alpha-linked glycosylated amino acid block Fmoc-GalNacalpha-Ser / Thr-OH at a low temperature (-20 DEG C to 0 DEG C); then assembling the building blocks on a repeated polypeptide skeleton rich in proline, serine and threonine at accurate intervals (3-5 amino acid residues) through a solid-phase peptide synthesis technology to form a'sugar chain growth scaffold '; then, a series of high-specificity glycosyl transferases are used for carrying out sequential and directional sugar chain enzymatic extension on the scaffold, and a uniform core structure (such as a core type 3) is constructed; and finally, performing high-efficiency purification by a three-step series method of size exclusion chromatography, ion exchange chromatography and lectin affinity chromatography. The technical bottleneck of inhomogeneity (lt, 70%) of glycoforms in a traditional method is overcome, the mucoprotein core fragment with glycoform homogeneity larger than or equal to 95% and bioactivity highly similar to that of natural mucoprotein can be prepared on a large scale, and the method has wide application prospects in the biomedical fields of mucous membrane protective agents, drug delivery carriers and the like.

The invention provides non-naturally occurring microbial organisms containing enzymatic pathways and / or metabolic modifications for enhancing carbon flux through acetyl-CoA. In some embodiments, the microbial organisms of the invention having such pathways also include pathways for generating reducing equivalents, formaldehyde fixation and / or formate assimilation. The enhanced carbon flux through acetyl-CoA, in combination with pathways for generating reducing equivalents, formaldehyde fixation and / or formate assimilation can, in some embodiments, be used for production of a bioderived compound. Accordingly, in some embodiments, the microbial organisms of the invention can include a pathway capable of producing a bioderived compound of the invention. The invention still further provides a bioderived compound produced by a microbial organism of the invention, culture medium having the bioderived compound of the invention, compositions having the bioderived compound of the invention, a biobased product comprising the bioderived compound of the invention, and a process for producing a bioderived compound of the invention.

The invention provides a synergistic treatment pharmaceutical composition for preventing and treating recurrence after hepatocellular carcinoma thermal ablation and application of the synergistic treatment pharmaceutical composition. Aiming at malignant tumor progression driven by ACSS3 gene silencing after thermal ablation, an epigenetic editing technology is innovatively combined with metabolite supplementation, and a preparation for specifically activating or promoting ACSS3 gene expression and sodium propionate are cooperatively used, so that ACSS3 can be remarkably reactivated or expression can be promoted in a xenograft model derived from a cell line and a patient; the compound can be used for recovering propionyl-coenzyme A synthesis and normal propionic acid metabolism and inhibiting fatty acid beta-oxidation, so that the growth and metastasis of post-ablation hepatocellular carcinoma (HCC) are effectively blocked, and the limitation of a single therapy is overcome. A clearer mechanism target is provided for preventing recurrence after HCC thermal ablation, meanwhile, the prepared targeting nano-liposome preparation has a synergistic effect, and as a brand-new treatment strategy, the defect that intervention on recurrence roots is insufficient in the prior art is overcome.

The invention belongs to the technical field of biochemistry, and particularly relates to an efficient enzymatic synthesis method of holothurian glycosaminoglycan. According to the invention, a high-efficiency enzymatic synthesis approach of the sea cucumber glycosaminoglycan is established, sulfated fucose and chondroitin sulfate are used as raw materials, and the sea cucumber glycosaminoglycan with anticoagulant activity is efficiently prepared by using the alpha 1, 3-fucosyltransferase mutant and L-fucose kinase / GDP-fucose pyrophosphorylase and adopting a one-pot multi-enzyme synthesis strategy. And important technical support is provided for development of novel anticoagulant drugs.

The application discloses a cytochrome P450BM3 mutant, a carrier, microorganisms and application thereof, and belongs to the technical field of chemical enzyme synthesis.The application discloses a synthetic route of a key drug intermediate dehydro-nandrolone acetate synthesized by a chemical enzyme method, wherein enzyme catalysis and chemical catalysis are included.The enzyme catalysis includes a cytochrome P450 mutant and 17beta-steroid reductase, and a one-pot two-step method is used to catalyze acid to be removed to generate 7beta-hydroxynandrolone.Combined with chemical catalysis, the 7beta-hydroxynandrolone is subjected to dehydration esterification to generate the dehydro-nandrolone acetate.The synthetic route has short steps, high yield and the ability to reduce the production cost of related steroid drugs.

The present invention relates to a T7 RNA polymerase mutant and its application. Specifically, the present invention discloses a T7 RNA polymerase mutant, which has at least one amino acid mutation at a site corresponding to the wild-type T7 RNA polymerase selected from the group consisting of: position 44, position 45, position 46, position 172, position 389, position 425, position 441, position 632, position 643, position 754, and position 789; the numbering of the mutation sites is based on the sequence shown in SEQ ID NO:1, and the mutation results in a significant reduction in the by-product dsRNA produced during the process of the enzyme synthesizing mRNA, and at the same time reduces the production of RNA aggregates.

This invention discloses a method and system for controlling the preparation of low-temperature, high-efficiency straw-promoting microbial agents, relating to the field of microbial agent preparation process control technology. It utilizes multiple types of sensors to collect a microbial agent preparation dataset RAW(t) at time t, calculates and constructs a metabolic feature vector Vm(t) at time t, achieving real-time quantification of the macroscopic metabolic activity of microorganisms. Furthermore, it calculates the respiratory entropy Q at time t. R (t) and calculate the enzyme synthesis potential E at time t. S (t) and construct the diagnostic state vector Ds(t) at time t, thereby transforming external measurable parameters into a deep insight into the internal production potential of the microbial cells, objectively and dynamically diagnosing the fermentation process into different physiological stages, and generating a stage identifier ID(t) at time t, automatically executing the matching fine control strategy AIR, ensuring that optimal regulation is automatically adopted at each stage, thereby significantly improving the quality, production efficiency and batch stability of the microbial agent product.

The invention discloses a plant source leuco-anthocyanidin dioxygenase mutant and application thereof in synthesis of chiral drug intermediates, and belongs to the technical field of enzymology engineering. According to the invention, leuco-anthocyanidin dioxygenase (LDOX) derived from arabidopsis thaliana is modified through site-specific mutagenesis, an F304L / T239S double mutant (LDOXLS) is obtained, the (R)-phenylglycine analogue can be synthesized with the yield of 1.69 g / L, the yield of 81% and the enantiomeric excess ratio of 97: 3, and different substituent derivatives of the (R)-phenylglycine analogue can be generated. Compared with the traditional method, the method for synthesizing the (R)-phenylglycine analogue and the derivative thereof by using the enzyme has the advantages of wider substrate range, higher stereoselectivity, more environment-friendly requirement, simplicity and convenience in operation and the like.

The invention relates to the technical field of gene engineering, in particular to high-activity peptide ligase and application thereof in synthesis of tilpotide. The high-activity peptide ligase provided by the invention shows catalytic efficiency far higher than that of an existing enzyme in a specific ligation reaction aiming at an acyl donor and an acyl receptor of a precursor fragment of the tilpoitide; by systematically transforming a host strain, the high-activity peptide ligase which can be secreted and expressed at high level and high fidelity and has correct folding and complete activity is obtained, and meanwhile, the degradation of a product is reduced to the maximum extent; and an optimal expression element combination of the high-activity peptide ligase is obtained through high-throughput optimization of an expression system. The method is finally integrated into a complete new process for synthesizing the tilpoitide by the chemical enzyme method, which can realize kilogram-level production, and is suitable for popularization and application.

The invention belongs to the field of biochemical engineering, and relates to a preparation method of glutathione, in particular to a method for synthesizing, separating and extracting glutathione by using double enzymes. Main impurities in the enzyme catalysis reaction liquid can be effectively removed through the alcohol solvent precipitation method, oxidation in the separation and extraction process can be slowed down, the alcohol solvent can be recycled, and cost can be saved. According to the method, most of macromolecular and micromolecular impurities can be effectively removed through an ultrafiltration method, and the purity of the product can be increased. The preparation method of glutathione is simple and convenient to operate, low in pollution, mild in reaction condition and suitable for industrial production.

The invention discloses an enzymatic synthesis method of a melogabalin intermediate and related alcohol dehydrogenase, racemic 3-ethyl bicyclo [3.2. 0] hept-3-ene-6-ketone is used as a substrate, a genetically engineered bacterium for producing alcohol dehydrogenase is used as an enzyme catalyst, a genetically engineered bacterium for producing formate dehydrogenase, ammonium formate and coenzyme are matched, an enzymatic reaction is carried out under a stirring condition, and the melogabalin intermediate is obtained. And carrying out purification and separation on a reaction product to obtain the melogabalin intermediate (1R, 5S)-3-ethyl bicyclo [3.2. 0] hept-3-ene-6-ketone. The method has the advantages of simple reaction, few steps, mild reaction, less pollution and the like, and has great industrial application potential and commercial value.

This invention discloses a method for designing optimal strains for product synthesis using an enzyme-constrained model combined with an evolutionary algorithm, comprising the following steps: Step S1, solving the enzyme-constrained model with the objective function of maximizing the specific growth rate of the strain to obtain the simulated metabolic flux of the wild-type strain; Step S2, performing dimensionality reduction analysis and labeling on genes directly related to enzyme synthesis within the model by solving a series of flux balance analysis problems with fixed biomass synthesis rates; Step S3, predicting the yield of single-target editing; Step S4, using a genetic algorithm to search for combined targets after dimensionality reduction, adjusting relevant parameters to obtain the optimal gene editing combination strategy; Step S5, repeating the experiment to verify the stability of the algorithm. This invention utilizes genetic algorithms and enzyme-constrained models to discover non-intuitive gene editing combination strategies, providing a new method for constructing efficient microbial cell factories for synthetic chemicals, and demonstrating the potential of applying heuristic algorithms to strain optimization design.

The invention discloses streptomyces hygroscopicus ascomycetes subspecies and an obtaining method thereof. Regulating genes fkbN, fkbR1 and fkbR2 on an ascomycin biosynthesis gene cluster of the streptomyces hygroscopicus ascomycetes subspecies ATCC 14891 and a gene fkbS participating in synthesis of an ascomycin precursor ethyl malonyl coenzyme A are subjected to biosynthesis to obtain the streptomyces hygroscopicus ascomycetes subspecies. And carrying out overexpression on a regulatory gene SH03686 of a leucine response regulatory protein family (Lrp) outside the gene cluster, and screening an ascomycin high-yield strain. According to the invention, after combined overexpression of genes fkbS and fkbN and overexpression of a gene fkbR2 are carried out by a strain SN9 and a strain R2-17 in starting bacteria, the transcriptional level of regulatory genes (fkbN, fkbR2) on an ascomycin biosynthesis gene cluster and related genes participating in synthesis of precursors ethyl malonyl coenzyme A (fkbS, fkbE), piperidine acid (fkbL) and DHCHC (fkbO) are improved, and finally, the ascomycin yield is improved.

The present invention relates to a method for preparing oligosaccharides by a glycoside phosphorylase combined with monosaccharide kinase and polyphosphate kinase. The method comprises the steps of using donor monosaccharide, acceptor sugar, polyphosphate, and low equivalent adenosine phosphate as substrates, adding magnesium chloride to the substrate, and then co-catalyzing the reaction for 1.5 to 22 hours at 25 to 40 ° C by polyphosphatase, monosaccharide kinase and glycoside phosphorylase to generate crude oligosaccharide products, and obtaining oligosaccharides after purification. The present invention provides a method for preparing important oligosaccharides by using monosaccharides, acceptor substrates, polyphosphate, and low equivalent adenosine phosphate as substrates, and realizing ATP regeneration by using polyphosphate kinase as substrate, effectively solving the problem that a large amount of expensive sugar nucleotide donors and auxiliary factor ATP need to be added in the process of synthesizing oligosaccharides by bioenzymatic method, resulting in the problem that the cost is too high and cannot be mass-produced. The method of the present invention was successfully used to synthesize lactoyl-N-disaccharide, galactoyl-N-disaccharide, chitosan, and protein N-sugar chain core trisaccharide derivatives.

The invention provides a recombinant strain for synthesizing HMB, application of the recombinant strain, and a method for performing whole-cell catalytic synthesis of HMB by using the strain. The recombinant bacterium is obtained by knocking out genes such as a fatty acid metabolism regulation factor fadR gene, a beta-ketoacyl-ACP synthase II gene FabF, a beta-ketoacyl-ACP synthase III gene FabH and the like and enhancing or inserting genes such as a fatty acyl coenzyme A synthetase gene fadD, a long-chain fatty acid transporter gene fadL, a short-chain fatty acid degradation regulation gene cluster gene atoSC and the like. A strain capable of producing HMB by using fatty acid or a substance containing a fatty acid component as a raw material is obtained. The strain has the prospect of green, efficient and industrial production of HMB by utilizing the capability of synthesizing HMB.

The invention discloses serratia marcescens for producing terpenoids and application of the serratia marcescens, and belongs to the technical field of bioengineering. According to the invention, part of genes, such as acetoacetyl-coenzyme A thiolase, 3-hydroxy-3-methylglutaryl-coenzyme A synthetase, 3-hydroxy-3-methylglutaryl-coenzyme A reductase and phosphomevalonate decarboxylase, in a haloarchaea MVA pathway are integrated in chromosomes of a serratia marcescens host, and mevalonate kinase is expressed on plasmids, so that the recombinant serratia marcescens is obtained. Meanwhile, competitive genes are knocked out, and a universal terpenoid production strain is constructed. Synthetases of other terpenoids are further introduced into the universal terpenoid production strain and are used for synthesizing more terpenoids.