Method for recovering D-p-hydroxyphenylglycine (D-HPG) from cefprozil production waste liquid in enzyme synthesis process

A technology of p-hydroxyphenylglycine and cefprozil, which is applied in the field of medicine, can solve the problems of difficulty in collecting a single substance, and the adsorption effect is greatly affected by multiple factors, and achieve the effect of low processing cost and low energy consumption

Inactive Publication Date: 2015-11-25
AMICOGEN CHINA BIOPHARM CO LTD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] Chinese patent application CN102816803A discloses a method for recovering active ingredients in amoxicillin mother liquor, wherein the macroporous adsorption resin used is a porous adsorption material, which has a wide range of selective adsorption for substances, and the raw materials and finished products in the mother liquor are both It is a substance containing a benzene ring, which can be adsorbed to the resin by the van der Waals force, and then eluted step by step according to the different adsorption forces of each substance, but its adsorption is greatly affected by multiple factors, and it is difficult to elute. A single substance of good quality was collected
[0005] Although the ion exchange resin has a better single selectivity for substances, it is required that the single substance has better ion gain and loss ability than other substances, so there are certain limitations in the recovery of D-HPG in wastewater containing more impurities

Method used

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  • Method for recovering D-p-hydroxyphenylglycine (D-HPG) from cefprozil production waste liquid in enzyme synthesis process

Examples

Experimental program
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Effect test

Embodiment 1

[0034] The first step, resin pretreatment;

[0035] 201×7 resin (produced by Shandong Lukang Like Pharmaceutical Co., Ltd.) was packed into a column of 30mL, and 5BV of 1mol / L HCl aqueous solution was used to pass through the resin, washed with 4.5BV of deionized water until the pH of the dripping solution was 6, and then 1mol / L 5BV of NaOH aqueous solution to exchange the resin into OH type, and wash with 5.8BV of deionized water until the dripping solution pH=8;

[0036] The second step is the recovery of D-HPG;

[0037] Take 180mL of cefprozil crystallization waste liquid synthesized by enzymatic method at a temperature of 25°C, pass it through a 201×7 resin column at a flow rate of 1BV / h, and drip out 168mL, which can be recycled and applied to the next batch of production;

[0038] The third step, the elution of D-HPG;

[0039] Adopt 3BV deionized water to pass through the resin with a flow rate of less than 2BV / h, wash off the water-soluble residue on the resin; then u...

Embodiment 2

[0042] The first step, resin pretreatment;

[0043]201×7 resin (produced by Shandong Lukang Like Pharmaceutical Co., Ltd.) was packed into a column of 30mL, and 5BV of 1mol / L HCl aqueous solution was used to pass through the resin, washed with 6BV of deionized water until the pH of the dripping liquid was 5, and then 1mol / L of HCl was used to 5BV of NaOH aqueous solution to exchange the resin into OH type, and wash with 6BV of deionized water until the dripping solution pH=7;

[0044] The second step is the recovery of D-HPG;

[0045] Take 180mL of cefprozil crystallization waste liquid synthesized by enzymatic method at a temperature of 23°C, pass it through a 201×7 resin column at a flow rate of 1BV / h, and drop out 175mL, which can be recycled and applied to the next batch of production;

[0046] The third step, the elution of D-HPG;

[0047] Adopt 5BV deionized water to pass through the resin with a flow rate of less than 2BV / h, wash off the water-soluble residue on the r...

Embodiment 3

[0050] The first step, resin pretreatment;

[0051] 201×7 resin (produced by Shandong Lukang Like Pharmaceutical Co., Ltd.) was packed into a column of 30mL, and 5BV of 1mol / L HCl aqueous solution was used to pass through the resin, washed with 4-8BV of deionized water until the pH of the dripping solution was 5, and then 1mol / L 5BV of NaOH aqueous solution of L to exchange the resin into OH type, and wash with 6.4BV of deionized water until the dripping solution pH=8;

[0052] The second step is the recovery of D-HPG;

[0053] Take 180mL of cefprozil crystallization waste liquid synthesized by enzymatic method at a temperature of 23°C, pass it through a 201×7 resin column at a flow rate of 1BV / h, and drop out 172mL, which can be recycled and applied to the next batch of production;

[0054] The third step, the elution of D-HPG;

[0055] Use 4BV deionized water to pass through the resin at a flow rate of less than 2BV / h to wash off the water-soluble residue on the resin; the...

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Abstract

The invention discloses a method for recovering D-p-hydroxyphenylglycine (D-HPG) from a cefprozil production waste liquid in an enzyme synthesis process, which comprises the following two steps: 1. separating D-HPG from the crystallization waste liquid through ion exchange by using an ion exchange resin; and 2. purifying and eluting the D-HPG by using hydrochloric acid solutions with different concentrations under the actions of ion exchange and static electricity, and drying to obtain the finished product. The method is characterized in that the process of separating and purifying the D-HPG from the cefprozil production waste liquid in the enzyme synthesis process is completed by using the ion exchange resin, and meanwhile, the subsequent waste liquid treatment technique of the enzyme-process cefprozil synthesis is completed.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to the enzymatic synthesis of cefprozil, in particular to the recovery of D-p-aminophenylglycine in the waste liquid of enzymatic synthesis of cefprozil. Background technique [0002] As the main clinical product of the second-generation cephalosporin, cefprozil is the first oral cephalosporin antibiotic approved by the US FDA for the treatment of otitis media and sinusitis in children. Its biggest advantage in the pediatric drug market is its safety sex. Cefprozil has high bioavailability, complete absorption in the gastrointestinal tract of the human body, broad antibacterial spectrum, and good stability. It is a semi-synthetic cephalosporin drug with good development prospects, and its preparations have a very good market prospect in China. [0003] Due to the huge potential of cefprozil in the market, domestic scholars and research institutes have developed many enzymatic method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C229/36C07C227/40
Inventor 刘庆文林炳旺李建国王玲赵新祥
Owner AMICOGEN CHINA BIOPHARM CO LTD
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