Method for recovering D-p-hydroxyphenylglycine (D-HPG) from cefprozil production waste liquid in enzyme synthesis process
A technology of p-hydroxyphenylglycine and cefprozil, which is applied in the field of medicine, can solve the problems of difficulty in collecting a single substance, and the adsorption effect is greatly affected by multiple factors, and achieve the effect of low processing cost and low energy consumption
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Examples
Embodiment 1
[0034] The first step, resin pretreatment;
[0035] 201×7 resin (produced by Shandong Lukang Like Pharmaceutical Co., Ltd.) was packed into a column of 30mL, and 5BV of 1mol / L HCl aqueous solution was used to pass through the resin, washed with 4.5BV of deionized water until the pH of the dripping solution was 6, and then 1mol / L 5BV of NaOH aqueous solution to exchange the resin into OH type, and wash with 5.8BV of deionized water until the dripping solution pH=8;
[0036] The second step is the recovery of D-HPG;
[0037] Take 180mL of cefprozil crystallization waste liquid synthesized by enzymatic method at a temperature of 25°C, pass it through a 201×7 resin column at a flow rate of 1BV / h, and drip out 168mL, which can be recycled and applied to the next batch of production;
[0038] The third step, the elution of D-HPG;
[0039] Adopt 3BV deionized water to pass through the resin with a flow rate of less than 2BV / h, wash off the water-soluble residue on the resin; then u...
Embodiment 2
[0042] The first step, resin pretreatment;
[0043]201×7 resin (produced by Shandong Lukang Like Pharmaceutical Co., Ltd.) was packed into a column of 30mL, and 5BV of 1mol / L HCl aqueous solution was used to pass through the resin, washed with 6BV of deionized water until the pH of the dripping liquid was 5, and then 1mol / L of HCl was used to 5BV of NaOH aqueous solution to exchange the resin into OH type, and wash with 6BV of deionized water until the dripping solution pH=7;
[0044] The second step is the recovery of D-HPG;
[0045] Take 180mL of cefprozil crystallization waste liquid synthesized by enzymatic method at a temperature of 23°C, pass it through a 201×7 resin column at a flow rate of 1BV / h, and drop out 175mL, which can be recycled and applied to the next batch of production;
[0046] The third step, the elution of D-HPG;
[0047] Adopt 5BV deionized water to pass through the resin with a flow rate of less than 2BV / h, wash off the water-soluble residue on the r...
Embodiment 3
[0050] The first step, resin pretreatment;
[0051] 201×7 resin (produced by Shandong Lukang Like Pharmaceutical Co., Ltd.) was packed into a column of 30mL, and 5BV of 1mol / L HCl aqueous solution was used to pass through the resin, washed with 4-8BV of deionized water until the pH of the dripping solution was 5, and then 1mol / L 5BV of NaOH aqueous solution of L to exchange the resin into OH type, and wash with 6.4BV of deionized water until the dripping solution pH=8;
[0052] The second step is the recovery of D-HPG;
[0053] Take 180mL of cefprozil crystallization waste liquid synthesized by enzymatic method at a temperature of 23°C, pass it through a 201×7 resin column at a flow rate of 1BV / h, and drop out 172mL, which can be recycled and applied to the next batch of production;
[0054] The third step, the elution of D-HPG;
[0055] Use 4BV deionized water to pass through the resin at a flow rate of less than 2BV / h to wash off the water-soluble residue on the resin; the...
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Description
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Application Information
- IPC
- C07C229/36; C07C227/40
- Inventors
- 刘庆文; 林炳旺