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Method of synthesizing diacylglycerol

A technology of diglyceride and partial glyceride, applied in the field of diglyceride synthesis, can solve the problems of weak esterification activity, low esterification activity and the like

Active Publication Date: 2020-02-11
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the esterification activity of monoglyceride lipase is generally weak, especially the esterification activity of long-chain fatty acids is extremely low, and there is no report that it can be used to catalyze the esterification of long-chain fatty acids to prepare glycerides.

Method used

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  • Method of synthesizing diacylglycerol
  • Method of synthesizing diacylglycerol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1G 50 +GMGL

[0024] Get 4.3210g fatty acid and 5.6790g glycerin (molar ratio is 1:4) and the phosphate buffer solution that the pH of 0.4g is 7.5, add in the Erlenmeyer flask with stopper and mix uniformly, and be placed on the constant temperature magnetic stirrer that rotating speed is 500rpm 35 Preheat at ℃ for 10 minutes, add 240U / g partial glyceride lipase Lipase G after the preheating 50 (Based on the total mass of the reactant, purchased from Japan Amano Enzyme Co., Ltd.), while adding 240U / g of monoglyceride lipase GMGL, controlling the reaction temperature to be 35°C, and reacting for 12 hours, the esterification product DAG content was 49.50%. Molecular distillation separation is used for further separation and purification, and the DAG content is as high as 98.07%.

Embodiment 2

[0025] Example 2 SMG1+GMGL

[0026] Take 4.3210g of fatty acid and 5.6790g of glycerin (molar ratio is 1:4) and 0.4g of pH 7.5 phosphate buffer solution, add it into a stoppered Erlenmeyer flask and mix evenly, and place it on a constant temperature magnetic stirrer with a rotation speed of 500rpm at 35°C Preheat for 10 minutes, add 240U / g partial glyceride lipase SMG1 (based on the total mass of reactants) after preheating, and add 240U / g monoglyceride lipase GMGL at the same time, control the reaction temperature to 35°C; react for 12 hours , the DAG content of the esterification product is 50.04%, and further separated and purified by molecular distillation, the DAG content is as high as 98.30%.

Embodiment 3

[0027] Example 3G 50 +GMGL

[0028] Get 4.3210g fatty acid and 5.6790g glycerol (molar ratio is 1:4) and the pH of 0.2g is the phosphate buffer solution of 7.5, add in the Erlenmeyer flask with stopper and mix uniformly, and be placed on the constant temperature magnetic stirrer that rotating speed is 500rpm 35 Preheat at ℃ for 10 minutes, add 240U / g partial glyceride lipase Lipase G after the preheating 50 (Based on the total mass of reactants), while adding 240U / g of monoglyceride lipase GMGL, controlling the reaction temperature to 35°C, and reacting for 12 hours, the content of DAG in the esterified product was 45.50%, which was further separated and purified by molecular distillation.

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Abstract

The invention discloses a method of synthesizing diacylglycerol. The method is characterized in that a fatty acid donor, glycerol, partial glyceride lipase and monoglyceride lipase are mixed with water, an esterification reaction is conducted for 8-24 h, separation and purification are further conducted, and then diacylglycerol is obtained. According to the method of synthesizing diacylglycerol, the monoglyceride lipase is utilized for promoting the reaction efficiency of the partial glyceride lipase during the esterification reaction to increase the speed of diacylglycerol synthesis; comparedwith a method using one enzyme, the method of synthesizing diacylglycerol shortens reaction time by 50% or above; and after the esterification reaction, the yield of diacylglycerol reaches 45.50% orabove, and basically no triglyceride is generated in products, so that after molecular distillation and purification, the content of DAG reaches up to 98% or above. The method of synthesizing diacylglycerol is a synthesis method which is rapid, efficient, environmentally friendly and convenient for industrial production.

Description

technical field [0001] The invention relates to a method for synthesizing diglycerides. Background technique [0002] Diacylglycerol (DAG) is the esterification product of two hydroxyl groups on glycerol and fatty acid. It is a natural component of oil and an intermediate product of oil metabolism. There are two types of naturally occurring DAG, which can be divided into two isomers, 1,2-DAG and 1,3-DAG, according to the positions of vacant hydroxyl groups. Because the metabolic pathway of diglyceride is quite different from that of triglyceride, DAG has the functions of lowering blood lipid, relieving diabetes and its complications, and inhibiting fat accumulation. It is a healthy and safe functional oil. [0003] Diacylglycerol can be prepared by a variety of processes, mainly including hydrolysis, esterification, and glycerol hydrolysis. The hydrolysis method uses refined animal and vegetable oils as raw materials to select sn-1, 3 specific lipases to hydrolyze animal a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/6454C12Y301/01034C12N9/20C12R2001/10C12R2001/645
Inventor 王永华刘萱杨博王卫飞蓝东明罗日明
Owner SOUTH CHINA UNIV OF TECH
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