91results about How to "Increase synthesis rate" patented technology

Nutritive proteins are provided. In some embodiments the nutritive proteins comprise a protein digestibility corrected amino acid score (PDCAAS) that exceeds a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

The invention belongs to the technical field of microbes, and in particular relates to an antimicrobial compound and a fermenting preparation method and application thereof. The molecular weight of the antimicrobial compound of the invention is 445, the molecular formula of the antimicrobial compound is C16H23N5O10, the chemical name of the antimicrobial compound is 1-uracil-4-sarkosyl-serylamido-1,4-dideoxy-beta-D-glucopyranosyl acid, and the antimicrobial compound belongs to uracil hexose dipeptide and is named xin'aomycin. The preparation method adopts actinomycetes Streptomyces noursei and genetically improved strains thereof, and comprises the following steps: culturing in a primary liquid culture medium as a seed liquid; inoculating the cultured seed liquid to a secondary liquid culture medium B for culturing, and carrying out fed-batch fermentation culture of fed-batch liquid C; and after fermentation is finished, separating and purifying the structural compound from the fermentation culture liquid. The antimicrobial compound is used for manufacturing pesticide or medicine products. The invention has the characteristics of high yield, simple process and easy industrialization.

The invention belongs to the field of polymer materials, relating to a method for synthesizing acetoacetate ester modified polymer. The method comprises the following steps: taking polymer containing hydroxyl and micromolecule acetoacetate ester as raw materials, and performing reaction under the condition of microwave irradiation; and the equation is shown in the specification, wherein R2(OH)n is polymer containing multi-hydroxy. The invention synthesizes the polymer of which side chains or chain ends have a plurality of acetoacetate ester groups by the process of catalytic ester exchange. The synthetic method has the advantages of high efficiency, mild synthetic method and high safety. In addition, in the method, the catalyst can be added, thereby improving synthetic efficiency; the synthetic rate of the method is greatly improved compared with the prior art; and the reaction time is shortened to 2-30min.

The invention discloses an integrated quantum dot synthesizing method and device based on a micro-channel ion pump. The device comprises a first precursor solution container (7), a first one-way valve (8), a Y-shaped tube (9), a quantum dot solution collector (10), a reaction cavity (11), a second precursor solution container (12) and a second one-way valve (13); and the reaction cavity (11) comprises a precursor solution guide-in channel (1), an plate electrode (2), a micro-groove corrugated plate (4) and a quantum dot solution guide-out channel (3). Direct-current voltage is applied to the plate electrode (2), a strong electric field is formed in a micro-channel of the micro-groove corrugated plate (4), the ion pump effect is achieved, a mixed precursor solution flows into the micro-channel in an orientated manner and reacts, and an obtained quantum dot solution also flows out of the micro-channel in an orientated manner under the ion pump effect. Power and a reaction environment are integrated, synthesis speed of quantum dots is increased, and the device has the advantages of controllable reaction, cost conservation and the like.

The invention provides a device and a method for synthesizing ammonia by utilizing renewable energy sources, and relates to the technical field of ammonia synthesis. The device provided by the invention comprises an energy microgrid 1, a water electrolysis hydrogen production device 2, an air separation device 3, a first buffer tank 4, a first compressor 5, a second buffer tank 6, a third buffer tank 18, a heat exchanger 7, a reaction tower 8, a first adsorption column 17, a second adsorption column 11, a third adsorption column 20, a first flash tower 10, a second flash tower 14, a third flash tower 15, a liquid ammonia pump 16, a second compressor 12 and valves. According to the invention, the energy microgrid is used for providing a renewable energy source for hydrogen production by water electrolysis and nitrogen production by air separation, then the prepared hydrogen and nitrogen are mixed and then subjected to a synthetic reaction, and the generated high-temperature outlet gas is subjected to adsorption separation again after flash evaporation, condensation and separation, so that ammonia is obtained. The method can realize production of carbon-free ammonia, and the method combining adsorption separation and condensation separation can improve the nitrogen-hydrogen conversion rate and the ammonia synthesis rate in the ammonia synthesis reaction.

The invention discloses a method for improving the fermentation yield of hyaluronic acid (HA), and aims to solve the problems that the yield of the HA is restricted under the influence of the physiological metabolic properties of streptococcus zooepidemicus serving as production strains, while the yield of the HA produced by a microbial fermentation method is required to be improved and the production cost is required to be reduced imperatively at present. The method comprises the following steps: slant culture, seed culture, fermentation culture, first-stage culture and second-stage culture. By utilizing the method, the fermentation yield of the HA can be further improved and the fermentation comprehensive cost is reduced in the mode of combining fed-batch culture and intermittent pH stress, so that the application of the HA in the field of medicaments, makeup and food healthcare is promoted. By utilizing the method, the conventional fermentation culture mode is subverted; the problem that the yield cannot be further improved in the method for producing the HA is effectively solved; the method has important significance to improve the fermentation yield of the HA, reduce the production cost of enterprises and promote the application of the HA.

The invention discloses a method of synthesizing diacylglycerol. The method is characterized in that a fatty acid donor, glycerol, partial glyceride lipase and monoglyceride lipase are mixed with water, an esterification reaction is conducted for 8-24 h, separation and purification are further conducted, and then diacylglycerol is obtained. According to the method of synthesizing diacylglycerol, the monoglyceride lipase is utilized for promoting the reaction efficiency of the partial glyceride lipase during the esterification reaction to increase the speed of diacylglycerol synthesis; comparedwith a method using one enzyme, the method of synthesizing diacylglycerol shortens reaction time by 50% or above; and after the esterification reaction, the yield of diacylglycerol reaches 45.50% orabove, and basically no triglyceride is generated in products, so that after molecular distillation and purification, the content of DAG reaches up to 98% or above. The method of synthesizing diacylglycerol is a synthesis method which is rapid, efficient, environmentally friendly and convenient for industrial production.

The invention discloses a method for preparing 2-phenylethyl alcohol, which comprises the following steps: adding active saccharomyces cerevisiae in a biotransformation medium, taking L-phenylalanine as a substrate and glucose as a carbon source, adding an anti-oxidant, and fermenting under aerobic condition to obtain 2-phenylethyl alcohol. According to the invention, L-phenylalanine is taken as the substrate, glucose is taken as carbon source, under an alternative oxidation degraded system of glucose and ethanol, the anti-oxidant with certain concentration is added, and perfume 2-phenylethyl alcohol can be obtained through batch fermentation. The method increases the synthesis rate of products 2-phenylethyl alcohol, shortens biotransformation period; employs a method for disposable addition of the anti-oxidant to reduce the content of impurities such as butanols, isobutyric acid, butyric acid and isovaleric acid during the biotransformation process, and increases the 2-phenylethyl alcohol fragrance quality.

Nutritive proteins comprising no phenylalanine (Phe) are provided. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in a benchmark protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in a benchmark protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things. The compositions are useful, for example, to provide protein in the diet of subjects with a disorder characterized by accumulation of Phe in the body.

The invention is a kind of lithium ion cell positive material, which concretely refers to a manufacturing method for a kind of lithium manganic acid material which has an even spinel structure. The invention uses high purity electrolyzation MnO2 or / and chemical MnO2 as materials, the MnO2 with multi-aperture skeleton can be acquired through baking in 500-1000oC, acid processed for 0.5-3 hours in 70-100 oC, the block beforehand mixture; then they are synthesized for 8-30 hours in air under temperature of 700-900 oC, the product can acquired after being cooled and grinded. The material has a large capacity, the electrochemical performance is stable, and the cost is low, especially applicable to the lithium ion cell.

The invention relates to the technical field of biology, in particular to a thermostable reverse transcriptase to which a DNA binding protein derived from a high-temperature-resistant eosinophile is connected, the thermostability of the reverse transcriptase can be improved, and higher continuous synthesis capacity and template binding capacity are obtained.

The embodiment of the invention provides a singing synthesis method and device. The singing synthesis method comprises the steps of obtaining a target song and to-be-simulated user voice; acquiring song features of the target song and acoustic features of the user voice; based on the song features, the acoustic features and a pre-trained synthesis model, determining a plurality of acoustic parameters for synthesizing the song; and synthesizing the singing sound of the user based on the plurality of acoustic parameters. Thus, the song features of the pre-processed target song and the acoustic features of the user voice can be obtained, the acoustic parameters are determined by using the synthesis model, and the singing sound is synthesized by using the acoustic parameters, so that automaticsinging sound synthesis can be realized through model learning in singing sound synthesis, the singing sound synthesis rate is improved, synthesis factors are rich, and the trueness of the synthesized singing sound is high.

The invention discloses a method for producing glucosamine through fermentation of escherichia coli. After the escherichia coli, bacillus subtilis and staphylococcus are subjected to seed activation,a mixed fermentation agent is prepared, the mixed fermentation agent is inoculated into a fermentation medium containing glucose and a nitrogen source for constant-temperature shaking table culture, the fermentation process is carried out in a constant-speed material supplementing mode, the fermentation pH is controlled to be 6.8-7.0 through ammonia water, thus fermentation liquid containing the glucosamine is obtained, and the glucosamine is obtained after separation and purification. The fermentation activity of the escherichia coli is improved through the mixed fermentation agent, through addition of cysteine and alanine, the production speed of glutamic acid in the fermentation process can be decreased, and the synthesis rate of the glucosamine is increased.

The invention discloses a method for synthesizing and preparing high-quality methyl salicylate. An original concentrated-sulfuric-acid catalyst is replaced with a new catalyst. The new catalyst is prepared from base materials, polysaccharide, sulfide, talcum powder and addition agent, wherein base materials are prepared in the mode that silicon dioxide, kieselguhr and aluminum oxide are stirred and mixed; polysaccharide is one or more of poly-N-acetylglucosamine, galactose and polyfructosan; sulfide is one or two of potassium hydrogen sulfate and copper sulfate; addition agent is one or two of polyving akohol and polysorbitol. The new catalyst is stable in quality, even in concentration, steady in preparing-process operation, low in preparing cost, small in toxicity, good in repeatability, easy to operate and suitable for industrial mass production; meanwhile, the method replaces the traditional methyl salicylate synthesizing and preparing method with concentrated sulfuric acid as the catalyst, and the catalytic effect is obvious; meanwhile, the synthetic speed of the methyl salicylate is increased, and reaction time is shortened.

The invention belongs to the technical field of high-voltage ceramic dielectric capacitor materials, and provides a lead-free high-voltage medium-temperature sintered strontium bismuth titanium-baseddielectric material and a preparation method thereof. The strontium bismuth titanium-based dielectric material is prepared from the following raw materials: SrCO3, TiO2, Bi2O3 and a metal oxide. The strontium bismuth titanium-based dielectric material can meet the requirements of a high-voltage ceramic capacitor and a negative temperature compensation type capacitor on the dielectric property of the material at the same time, and is wider in application range; and medium-temperature sintering can be realized only by adding a common sintering aid without synthesizing cooling glass in advance oradding low-melting-point oxides such as lithium and boron. The preparation method comprises the following steps: firstly, pre-synthesizing a sintered block by adopting a solid-phase synthesis method,then preparing materials for the second time, ball-milling, drying, preparing a sample, and sintering at a medium temperature in an air atmosphere. The preparation method is simple in production andpreparation process and suitable for industrial production.

The invention discloses a special reaction device for a curing agent for repair of soil heavy metal pollution and a production method thereof. The reaction device comprises a sealing cover, a sealing lock, a reaction tank, a cleaning door, a finished product storage device, a finished product discharge solenoid valve, a reaction exhaust gas discharge solenoid valve, a reaction exhaust gas collecting tank, a control device and a finished product liquid level detector; a reaction raw material after crushing enters the reaction tank and generates a synthesis effect with a catalyst; generated reaction exhaust gas enters the reaction exhaust gas collecting tank through a pipeline and is collected and stored, a finished product generated in the synthesis process is discharged through the finished product storage device, and residues obtained after reaction and generated after the synthesis effect are manually cleaned through the cleaning door. The special reaction device for the curing agent for repair of soil heavy metal pollution has the advantages of energy saving, environmental protection, simple structure, high automation degree and high synthetic speed.

The invention discloses a high-quality hatching method for greylag. The high-quality hatching method is characterized by comprising the first step of hatching egg processing, the second step of hatching medium preparing, the third step of hatching egg hatching and the hatching egg hatching out. According to the high-quality hatching method, scientific and reasonable fine management is carried out on hatching conditions of hatching eggs and conforms to the hatching egg hatching rule of the greylag, the hatching period is remarkably shortened, young greylag can be hatched out within 21-23 days, the hatchability is increased well and stabilized to be 95% or above, and the hatched young greylag has the advantages of being strong in physique, large in size and full of animal spirits.

The invention discloses a fermentation method for producing N-acetylneuraminic acid and relates to the technical field of N-acetylneuraminic acid production. The fermentation method for producing N-acetylneuraminic acid comprises the steps of adding supplementary feed liquid to a fermentation culture medium inoculated with N-acetyl-neuraminic acid bacteria during fermentation, wherein the feed liquid contains glycerin and an additive, the additive is selected from one or more of N-acetylglucosamine and polyphosphoric acid substances. The fermentation method improves the synthesis rate of N-acetylneuraminic acid and meanwhile improves the yield of the N-acetylneuraminic acid by optimizing the composition or use amount of the feed liquid.

The invention discloses a polycarboxylate water reducer composition for self-compaction high-workability concrete. The polycarboxylate water reducer composition is composed of the following components: allyl alcohol polyoxyethylene ether, an unsaturated acid, functional monomers, an oxidizing agent, a reducing agent and a chain transfer agent, wherein the unsaturated acid consists of citric acid,acetic anhydride, acrylic acid and methacrylic acid according to a mass ratio of (1-3):(1-2):(2-3):(5-10), and the functional monomers are nano-diatomaceous earth and sulfonated melamine formaldehyderesin. According to the invention, the water reducer is synthesized by the allyl alcohol polyoxyethylene ether and the unsaturated acid, so that mixing performance of concrete can be improved, meanwhile, steel bars can be prevented from rusting, and the service life of the concrete is prolonged. Mutual reactions between cementing materials can be promoted through the functional monomers, and workability of the concrete is enhanced.

The invention discloses a method for synthesizing ammonia through high-temperature electrolysis by taking low-concentration gas as fuel, which comprises the following steps: preparing a proton conductor solid oxide electrolytic tank consisting of a porous Ni-BZCY cathode, a BZCY electrolyte and a porous LSCF-BZCY anode; preparing a low-concentration gas component as a fuel, and preparing humid air as an anode gas; and introducing fuel into a cathode gas channel of a solid oxide electrolytic tank, introducing humid air into an anode gas channel of the solid oxide electrolytic tank, and carrying out ammonia synthesis reaction on protons and nitrogen in low-concentration gas at 700 DEG C through external voltage. The comprehensive utilization rate of the low-concentration gas can be increased, the product ammonia gas can directly or indirectly reduce carbon emission and hydrogen production energy consumption in the synthesis ammonia industry, carbon circulation in the fossil fuel synthesis ammonia industry can be achieved, and energy conservation and emission reduction of the whole synthesis ammonia system are promoted.

The invention belongs to the field of photocatalytic materials, and particularly relates to a BiVO4 interface photocatalyst as well as a preparation method and an application thereof. The photocatalyst comprises a BiVO4 substrate, a ligand monomolecular layer formed on the surface of the BiVO4 substrate, and noble metal loaded on the ligand monomolecular layer, wherein the ligand monomolecular layer is obtained through a condensation reaction between a ligand containing an electron donating group and the surface of a BiVO4 substrate, the noble metal is loaded on the ligand monomolecular layerthrough coordination with the electron donating group, and the BiVO4 interface photocatalyst is a hydrophobic photocatalyst. According to the invention, the local coordination environment and the overall surface / interface polarity of the photocatalyst are regulated and controlled, so that the enrichment of electrons on the reaction active sites of the photocatalyst and the change of the surface and interface characteristics of the material are realized. Therefore, the technical problems of low light quantum efficiency, insufficient photocatalytic reaction driving force, low photocatalytic H2O2synthesis efficiency and the like of BiVO4 in the prior art are solved.

The invention relates to a method for increasing synthesis rate of starch of wheat grain. The method comprises the step of evenly spraying a certain concentration of mixed solution of salicylic acid and Tween 40 to wheat blade backs on 15-20 days after wheat anthesis at nightfall in a sunny day, wherein the use level of the mixture solution is 30-40Kg / 666.7m<2>. The synthesis rate of the starch of the wheat grain treated by the method disclosed by the invention can be increased by 16.1-26.5 percent, the thousand seed weight can be increased by 3-6g, and the yield is increased by 20-40Kg / 666.7m<2>.

The invention discloses a highly-efficient preparation method for a strong-fluorescence graphene quantum dot. The highly-efficient preparation method comprises the following steps: with pyrene as a reactant precursor and concentrated nitric acid as a nitrating agent, mixing the pyrene with the concentrated nitric acid in proportion, subjecting a mixed solution to water-bath heating to a constant low temperature of 60 to 80 DEG C under a reflux state, and carrying out a nitration reaction so as to obtain an intermediate product namely 1,3,6-trinitropyrene; and dissolving the intermediate product and an alkaline substance namely hydrazine hydrate into a solvent in a certain proportion, carrying out ultrasonic crushing so as to obtain an ultrasonically-crushed suspension, then transferring the suspension to a high-pressure-resistant reaction kettle, carrying out a hydrothermal reaction so as to synthesize a graphene quantum dot solution with high quantum efficiency, and carrying out vacuum filtering, drying and collection so as to obtain a graphene quantum dot powder. The method provided by the invention has the advantages of simple and highly-efficient preparation process, low cost,high quantum efficiency, environmental protection, no generation of harmful wastes and convenience in large-scale production, and facilitates promotion of graphene quantum dot application.

The invention relates to a fermenting preparation method of oosporein. The method is characterized in that chaetomium cupreum and genetic improved strain CH-1 are utilized and processed by two-stage fermenting culture, and the culture medium is subjected to material supplementing and feeding, so that high carbon to nitrogen ratio in strain growing and product synthesizing can be maintained, and high-yield oosporein is obtained. The fermenting and extracting processes are simple; and the operation is convenient.

The invention belongs to the technical field of algae culture, and discloses a method for increasing the content of phycoerythrobilin in porphyridium. The method comprises the following steps that a porphyridium seed solution is inoculated into a reaction tank containing an artificial synthesis culture solution, the illumination intensity is controlled to be 4000-6000 Lux, the flow of introduced air is 0.3-0.5 vvm, culture is carried out at 22-25 DEG C, the light-dark ratio is 14:10, and culture is carried out for 3 d; then brassinolide and bionitrogen are added, the mixture is continuously cultured for 3-5 d, and the porphyridium is collected. The method increases the growth rate of the porphyridium and the content of phycoerythrin.

The invention provides a device for facilitating control of a substrate temperature in diamond synthesis equipment. The device comprises a substrate table and a substrate, the top of the substrate table is provided with a plurality of first heat conducting rods, the substrate is supported on the substrate table by the first heat conducting rods, and the cross sections of the first heat conductingrods are of polygonal structures. By arranging a plurality of the first heat conducting rods between the substrate table and the substrate for supporting, a worker can change the thermal conductivitybetween the substrate table and the substrate by changing the number of the first heat conducting rods, thereby achieving reasonable control of the heat dissipation capability of the substrate, sincethe cross sections of the first heat conducting rods are of the polygonal structures, no relative sliding occurs between the substrate table and the substrate, so that the thermal conductivity betweenthe substrate table and the substrate is away stable after the first heat conducting rods are well placed, and the control effect of the heat dissipation capability of the substrate is more reproducible. The device is applied to the field of the diamond synthesis equipment.

The invention discloses a near infrared-visible light face image synthesis method based on comparative learning and StyleGAN2, and the method comprises the steps: 1, collecting paired near infrared-visible light face images, and carrying out the unified face detection and normalization preprocessing, thereby obtaining a data set image; 2, introducing a comparative learning mechanism, and constructing a generative network model comprising a generator based on a StyleGAN2 structure, a discriminator and an image multilayer feature extraction block; 3, combining with a proper loss function and an optimization function, and training by using the training set image to generate a network model; and 4, inputting a near-infrared face image to be tested to test the generative network model, and finally synthesizing a corresponding visible light face image. The synthesized visible light image is closer to a real image, and the facial edge details and skin color information of the face image can be better restored, so that the visual effect of the synthesized image and the cross-modal face recognition performance are improved.

The invention provides a super-large plasmid construction kit, a super-large plasmid construction method and application of the super-large plasmid construction kit. The kit comprises a DNA fragment Fn to be assembled and a vector, wherein the vector includes an intermediate vector and / or an assembly vector. The kit further comprises a lambda bacteriophage Red recombination system. By adopting a mode of combining in-vitro assembly and in-vivo assembly, large-fragment DNA and the assembly vector are firstly assembled in vitro by adopting a recombinase system, and then the in-vitro assembly product and the lambda phage Red recombinant system are simultaneously transformed into escherichia coli for in-vivo assembly. The constructed kit combines the advantages of in-vitro assembly and in-vivoassembly, the in-vivo assembly capacity of escherichia coli is enhanced by adopting the lambda phage Red recombination system, the synthesis rate of super-large plasmids is increased, and the synthesis success rate of the super-large plasmids is remarkably increased.

The invention discloses a magnesium ion battery positive electrode material of a novel microstructure and a preparation method thereof, and belongs to the technical field of battery materials. The invention solves the problems of low reversible capacity, poor rate capability and the like of the electrode material of the existing magnesium ion battery positive electrode. The preparation method comprises the following steps: dissolving manganese oxide in a high-concentration sodium hydroxide solution environment, carrying out hydrothermal reaction and centrifugation, directly freeze-drying the product to prepare a nanosheet and nanowire blended NaMnO electrode material, and adding titanium oxide in the same preparation process to prepare the Ti-doped NaMnO electrode material. The obtained nanosheet and nanowire blended NaMnO electrode material has higher tap density, reversible capacity, rate capability and good cycling stability, and the reversible capacity, the rate capability and thecycling stability are further improved by doping the designed Ti-doped NaMnO electrode material.

The invention discloses an incubation method for shortening the incubation period of bar-headed gooses. The method comprises the following steps of 1, hatching egg disinfection, 2, incubation medium preparation, 3, hatching egg incubation, and 4, hatching egg hatching. According to the incubation method, incubation conditions are scientifically and reasonably managed finely and meet the law of bar-headed goose hatching egg incubation, the incubation period is remarkably shortened, young bar-headed gooses can be incubated within 23-25 days, the incubation rate is increased well, and the incubated young bar-headed gooses have the advantages of being strong in physique and full of animal spirits.