89results about How to "Increase synthesis rate" patented technology

The invention discloses a method for improving the fermentation yield of hyaluronic acid (HA), and aims to solve the problems that the yield of the HA is restricted under the influence of the physiological metabolic properties of streptococcus zooepidemicus serving as production strains, while the yield of the HA produced by a microbial fermentation method is required to be improved and the production cost is required to be reduced imperatively at present. The method comprises the following steps: slant culture, seed culture, fermentation culture, first-stage culture and second-stage culture. By utilizing the method, the fermentation yield of the HA can be further improved and the fermentation comprehensive cost is reduced in the mode of combining fed-batch culture and intermittent pH stress, so that the application of the HA in the field of medicaments, makeup and food healthcare is promoted. By utilizing the method, the conventional fermentation culture mode is subverted; the problem that the yield cannot be further improved in the method for producing the HA is effectively solved; the method has important significance to improve the fermentation yield of the HA, reduce the production cost of enterprises and promote the application of the HA.

The invention is a kind of lithium ion cell positive material, which concretely refers to a manufacturing method for a kind of lithium manganic acid material which has an even spinel structure. The invention uses high purity electrolyzation MnO2 or/and chemical MnO2 as materials, the MnO2 with multi-aperture skeleton can be acquired through baking in 500-1000oC, acid processed for 0.5-3 hours in 70-100 oC, the block beforehand mixture; then they are synthesized for 8-30 hours in air under temperature of 700-900 oC, the product can acquired after being cooled and grinded. The material has a large capacity, the electrochemical performance is stable, and the cost is low, especially applicable to the lithium ion cell.

The invention relates to the technical field of biology, in particular to a thermostable reverse transcriptase to which a DNA binding protein derived from a high-temperature-resistant eosinophile is connected, the thermostability of the reverse transcriptase can be improved, and higher continuous synthesis capacity and template binding capacity are obtained.

The embodiment of the invention provides a singing synthesis method and device. The singing synthesis method comprises the steps of obtaining a target song and to-be-simulated user voice; acquiring song features of the target song and acoustic features of the user voice; based on the song features, the acoustic features and a pre-trained synthesis model, determining a plurality of acoustic parameters for synthesizing the song; and synthesizing the singing sound of the user based on the plurality of acoustic parameters. Thus, the song features of the pre-processed target song and the acoustic features of the user voice can be obtained, the acoustic parameters are determined by using the synthesis model, and the singing sound is synthesized by using the acoustic parameters, so that automaticsinging sound synthesis can be realized through model learning in singing sound synthesis, the singing sound synthesis rate is improved, synthesis factors are rich, and the trueness of the synthesized singing sound is high.

The invention discloses a method for producing glucosamine through fermentation of escherichia coli. After the escherichia coli, bacillus subtilis and staphylococcus are subjected to seed activation,a mixed fermentation agent is prepared, the mixed fermentation agent is inoculated into a fermentation medium containing glucose and a nitrogen source for constant-temperature shaking table culture, the fermentation process is carried out in a constant-speed material supplementing mode, the fermentation pH is controlled to be 6.8-7.0 through ammonia water, thus fermentation liquid containing the glucosamine is obtained, and the glucosamine is obtained after separation and purification. The fermentation activity of the escherichia coli is improved through the mixed fermentation agent, through addition of cysteine and alanine, the production speed of glutamic acid in the fermentation process can be decreased, and the synthesis rate of the glucosamine is increased.

The invention discloses a method for synthesizing and preparing high-quality methyl salicylate. An original concentrated-sulfuric-acid catalyst is replaced with a new catalyst. The new catalyst is prepared from base materials, polysaccharide, sulfide, talcum powder and addition agent, wherein base materials are prepared in the mode that silicon dioxide, kieselguhr and aluminum oxide are stirred and mixed; polysaccharide is one or more of poly-N-acetylglucosamine, galactose and polyfructosan; sulfide is one or two of potassium hydrogen sulfate and copper sulfate; addition agent is one or two of polyving akohol and polysorbitol. The new catalyst is stable in quality, even in concentration, steady in preparing-process operation, low in preparing cost, small in toxicity, good in repeatability, easy to operate and suitable for industrial mass production; meanwhile, the method replaces the traditional methyl salicylate synthesizing and preparing method with concentrated sulfuric acid as the catalyst, and the catalytic effect is obvious; meanwhile, the synthetic speed of the methyl salicylate is increased, and reaction time is shortened.

The invention belongs to the technical field of high-voltage ceramic dielectric capacitor materials, and provides a lead-free high-voltage medium-temperature sintered strontium bismuth titanium-baseddielectric material and a preparation method thereof. The strontium bismuth titanium-based dielectric material is prepared from the following raw materials: SrCO3, TiO2, Bi2O3 and a metal oxide. The strontium bismuth titanium-based dielectric material can meet the requirements of a high-voltage ceramic capacitor and a negative temperature compensation type capacitor on the dielectric property of the material at the same time, and is wider in application range; and medium-temperature sintering can be realized only by adding a common sintering aid without synthesizing cooling glass in advance oradding low-melting-point oxides such as lithium and boron. The preparation method comprises the following steps: firstly, pre-synthesizing a sintered block by adopting a solid-phase synthesis method,then preparing materials for the second time, ball-milling, drying, preparing a sample, and sintering at a medium temperature in an air atmosphere. The preparation method is simple in production andpreparation process and suitable for industrial production.

The invention discloses a high-quality hatching method for greylag. The high-quality hatching method is characterized by comprising the first step of hatching egg processing, the second step of hatching medium preparing, the third step of hatching egg hatching and the hatching egg hatching out. According to the high-quality hatching method, scientific and reasonable fine management is carried out on hatching conditions of hatching eggs and conforms to the hatching egg hatching rule of the greylag, the hatching period is remarkably shortened, young greylag can be hatched out within 21-23 days, the hatchability is increased well and stabilized to be 95% or above, and the hatched young greylag has the advantages of being strong in physique, large in size and full of animal spirits.

The invention discloses a fermentation method for producing N-acetylneuraminic acid and relates to the technical field of N-acetylneuraminic acid production. The fermentation method for producing N-acetylneuraminic acid comprises the steps of adding supplementary feed liquid to a fermentation culture medium inoculated with N-acetyl-neuraminic acid bacteria during fermentation, wherein the feed liquid contains glycerin and an additive, the additive is selected from one or more of N-acetylglucosamine and polyphosphoric acid substances. The fermentation method improves the synthesis rate of N-acetylneuraminic acid and meanwhile improves the yield of the N-acetylneuraminic acid by optimizing the composition or use amount of the feed liquid.

The invention discloses a polycarboxylate water reducer composition for self-compaction high-workability concrete. The polycarboxylate water reducer composition is composed of the following components: allyl alcohol polyoxyethylene ether, an unsaturated acid, functional monomers, an oxidizing agent, a reducing agent and a chain transfer agent, wherein the unsaturated acid consists of citric acid,acetic anhydride, acrylic acid and methacrylic acid according to a mass ratio of (1-3):(1-2):(2-3):(5-10), and the functional monomers are nano-diatomaceous earth and sulfonated melamine formaldehyderesin. According to the invention, the water reducer is synthesized by the allyl alcohol polyoxyethylene ether and the unsaturated acid, so that mixing performance of concrete can be improved, meanwhile, steel bars can be prevented from rusting, and the service life of the concrete is prolonged. Mutual reactions between cementing materials can be promoted through the functional monomers, and workability of the concrete is enhanced.

The invention discloses a method for synthesizing ammonia through high-temperature electrolysis by taking low-concentration gas as fuel, which comprises the following steps: preparing a proton conductor solid oxide electrolytic tank consisting of a porous Ni-BZCY cathode, a BZCY electrolyte and a porous LSCF-BZCY anode; preparing a low-concentration gas component as a fuel, and preparing humid air as an anode gas; and introducing fuel into a cathode gas channel of a solid oxide electrolytic tank, introducing humid air into an anode gas channel of the solid oxide electrolytic tank, and carrying out ammonia synthesis reaction on protons and nitrogen in low-concentration gas at 700 DEG C through external voltage. The comprehensive utilization rate of the low-concentration gas can be increased, the product ammonia gas can directly or indirectly reduce carbon emission and hydrogen production energy consumption in the synthesis ammonia industry, carbon circulation in the fossil fuel synthesis ammonia industry can be achieved, and energy conservation and emission reduction of the whole synthesis ammonia system are promoted.

The invention relates to a method for increasing synthesis rate of starch of wheat grain. The method comprises the step of evenly spraying a certain concentration of mixed solution of salicylic acid and Tween 40 to wheat blade backs on 15-20 days after wheat anthesis at nightfall in a sunny day, wherein the use level of the mixture solution is 30-40Kg/666.7m<2>. The synthesis rate of the starch of the wheat grain treated by the method disclosed by the invention can be increased by 16.1-26.5 percent, the thousand seed weight can be increased by 3-6g, and the yield is increased by 20-40Kg/666.7m<2>.

The invention discloses a highly-efficient preparation method for a strong-fluorescence graphene quantum dot. The highly-efficient preparation method comprises the following steps: with pyrene as a reactant precursor and concentrated nitric acid as a nitrating agent, mixing the pyrene with the concentrated nitric acid in proportion, subjecting a mixed solution to water-bath heating to a constant low temperature of 60 to 80 DEG C under a reflux state, and carrying out a nitration reaction so as to obtain an intermediate product namely 1,3,6-trinitropyrene; and dissolving the intermediate product and an alkaline substance namely hydrazine hydrate into a solvent in a certain proportion, carrying out ultrasonic crushing so as to obtain an ultrasonically-crushed suspension, then transferring the suspension to a high-pressure-resistant reaction kettle, carrying out a hydrothermal reaction so as to synthesize a graphene quantum dot solution with high quantum efficiency, and carrying out vacuum filtering, drying and collection so as to obtain a graphene quantum dot powder. The method provided by the invention has the advantages of simple and highly-efficient preparation process, low cost,high quantum efficiency, environmental protection, no generation of harmful wastes and convenience in large-scale production, and facilitates promotion of graphene quantum dot application.

The invention provides a device for facilitating control of a substrate temperature in diamond synthesis equipment. The device comprises a substrate table and a substrate, the top of the substrate table is provided with a plurality of first heat conducting rods, the substrate is supported on the substrate table by the first heat conducting rods, and the cross sections of the first heat conductingrods are of polygonal structures. By arranging a plurality of the first heat conducting rods between the substrate table and the substrate for supporting, a worker can change the thermal conductivitybetween the substrate table and the substrate by changing the number of the first heat conducting rods, thereby achieving reasonable control of the heat dissipation capability of the substrate, sincethe cross sections of the first heat conducting rods are of the polygonal structures, no relative sliding occurs between the substrate table and the substrate, so that the thermal conductivity betweenthe substrate table and the substrate is away stable after the first heat conducting rods are well placed, and the control effect of the heat dissipation capability of the substrate is more reproducible. The device is applied to the field of the diamond synthesis equipment.

The invention provides a super-large plasmid construction kit, a super-large plasmid construction method and application of the super-large plasmid construction kit. The kit comprises a DNA fragment Fn to be assembled and a vector, wherein the vector includes an intermediate vector and/or an assembly vector. The kit further comprises a lambda bacteriophage Red recombination system. By adopting a mode of combining in-vitro assembly and in-vivo assembly, large-fragment DNA and the assembly vector are firstly assembled in vitro by adopting a recombinase system, and then the in-vitro assembly product and the lambda phage Red recombinant system are simultaneously transformed into escherichia coli for in-vivo assembly. The constructed kit combines the advantages of in-vitro assembly and in-vivoassembly, the in-vivo assembly capacity of escherichia coli is enhanced by adopting the lambda phage Red recombination system, the synthesis rate of super-large plasmids is increased, and the synthesis success rate of the super-large plasmids is remarkably increased.

The invention discloses a magnesium ion battery positive electrode material of a novel microstructure and a preparation method thereof, and belongs to the technical field of battery materials. The invention solves the problems of low reversible capacity, poor rate capability and the like of the electrode material of the existing magnesium ion battery positive electrode. The preparation method comprises the following steps: dissolving manganese oxide in a high-concentration sodium hydroxide solution environment, carrying out hydrothermal reaction and centrifugation, directly freeze-drying the product to prepare a nanosheet and nanowire blended NaMnO electrode material, and adding titanium oxide in the same preparation process to prepare the Ti-doped NaMnO electrode material. The obtained nanosheet and nanowire blended NaMnO electrode material has higher tap density, reversible capacity, rate capability and good cycling stability, and the reversible capacity, the rate capability and thecycling stability are further improved by doping the designed Ti-doped NaMnO electrode material.

The invention discloses an incubation method for shortening the incubation period of bar-headed gooses. The method comprises the following steps of 1, hatching egg disinfection, 2, incubation medium preparation, 3, hatching egg incubation, and 4, hatching egg hatching. According to the incubation method, incubation conditions are scientifically and reasonably managed finely and meet the law of bar-headed goose hatching egg incubation, the incubation period is remarkably shortened, young bar-headed gooses can be incubated within 23-25 days, the incubation rate is increased well, and the incubated young bar-headed gooses have the advantages of being strong in physique and full of animal spirits.

The invention discloses a preparation method of special foliage fertilizer for tobacco. The foliage fertilizer is prepared from components in parts by weight as follows: 1-3 parts of anhydrous ferroussulfate, 1-3 parts of zinc nitrate, 0.1-0.3 parts of malt selenium, 40-60 parts of a fermentation broth, 10-20 parts of monopotassium phosphate, 3-8 parts of potassium borate, 3-8 parts of a surfactant and 3-8 parts of an activator. The preparation method of the special foliage fertilizer for the tobacco has the benefits as follows: the special foliage fertilizer for the tobacco can timely supplement nutrients to relieve incomplete absorption caused by underdeveloped roots or root system function decline at the seedling stage and the later growth stage or inhibition of nutrient absorption ofthe roots in saline and alkaline, drought environments and the like and has functions of strengthening seedlings and increasing yield; the foliage fertilizer can promote increase of plant height of leafy vegetable crops and number of blades and promote blades to be stout, broad and bright in color; the foliage fertilizer increases the fruit-bearing percentage of fruiting tobacco, is favorable forexpanding fruits, enables the fruits to have smooth surfaces and bright color, enhances the taste of the fruits and can timely relieve the element deficiency symptoms of crops.

The invention discloses a method for preparing a composite water-retaining material with hydroxypropylated lobster shells as the main raw material. First, the lobster shells are used for high-temperature treatment in a high-pressure steam sterilizer, and then added to dilute hydrochloric acid solution for boiling treatment, ball milling into a fine powder, then dissolve it in sodium hydroxide solution, stir to dissolve it, freeze it, then thaw it, add tetramethylammonium hydroxide solution and propylene oxide to react, add eggshell powder and other ultrasonic reactions After processing vested. The water-retaining material prepared by the invention has high-efficiency water-retaining and moisturizing functions, and has high adsorption and slow-release effects, and can perform fertilizer adsorption and slow-release, thereby improving the utilization rate of fertilizers.