Method for preparing 2-phenylethyl alcohol

A technology of phenylethyl alcohol and phenylalanine, applied in the chemical industry, can solve the problems of low aroma quality of 2-phenylethyl alcohol and high impurity content of 2-phenylethyl alcohol, so as to shorten the biotransformation cycle, improve the conversion efficiency, and achieve remarkable technological progress. Effect

Inactive Publication Date: 2015-04-08
SHANGHAI INST OF TECH
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Problems solved by technology

[0005] Aiming at the technical problems in the prior art, the invention provides a method for preparing 2-phenylethanol, which solves the problems in the 2-phenylethanol obtained by the preparation method of the prior art. Technical problems of high impurity content and low aroma quality of 2-phenylethanol

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  • Method for preparing 2-phenylethyl alcohol

Examples

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Embodiment 1

[0018] The biotransformation medium used in this example consists of 10 g / L L-phenylalanine, 30 g / L glucose, and 5 g / L K 2 HPO 4 , 5 g / L MgSO 4 ·7H 2 It is composed of O, 1 g / L NaCl and the balance of water, pH 6.5.

[0019] After sterilizing the above-mentioned NBS BIOFLO 3000 mechanically stirred fermenter with 3.5L fermentation medium (L-phenylalanine high temperature and high drying sterilization) at 121°C for 20 minutes. After cooling, connect the fermentation tank, pass condensed water and sterile air with a ventilation volume of 0.5 V / (V·min), control the temperature of the fermentation tank at 28°C, and add sterilized L-phenylalanine. Inoculate 1% active Saccharomyces cerevisiae by flame burning at the inoculation port of the fermenter, and at the same time add 0.5g / L carotene at a speed of 350r / min for fermentation culture. After 4 hours, the speed was adjusted to 600r / min, the aseptic air ventilation rate was 1.0 V / V·min, and the control temperature remained unchanged a...

Embodiment 2

[0025] The biotransformation medium used in this example consists of 10g / L L-phenylalanine, 30g / L glucose, 5g / L K 2 HPO 4 , 5g / L MgSO 4 ·7H 2 O, 1g / L NaCl and the remainder of water, pH 6.5.

[0026] The above-mentioned fermentation medium containing 3.5L was sterilized by autoclaving at 121°C for 20 minutes. After cooling, connect the fermentation tank, pass condensed water and sterile air with a ventilation volume of 0.5 V / (V·min), control the temperature of the fermentation tank at 28°C, and add sterilized L-phenylalanine. Inoculate 1% active Saccharomyces cerevisiae by flame burning at the inoculation port of the fermenter, and add 0.5g / L vitamin E at the same time, and the rotation speed is 350r / min for fermentation culture. After 4 hours, the speed was adjusted to 600r / min, the ventilation rate of sterile air was 1.0 V / V·min, and the temperature was kept unchanged at 28°C. When both glucose and ethanol are consumed completely, the biotransformation is ended.

[0027] In the...

Embodiment 3

[0030] The biotransformation medium of this embodiment is calculated per liter, which consists of 10 g / L L-phenylalanine, 30 g / L glucose, and 5 g / L K 2 HPO 4 , 5g / L MgSO 4 ·7H 2 O, 1g / L NaCl and the remainder of water, pH 6.5.

[0031] The above-mentioned fermentation medium containing 3.5L was sterilized by autoclaving at 121°C for 20 minutes. Connect the fermentation tank, pass condensed water and sterile air with a ventilation volume of 0.5 V / (V·min), control the temperature of the fermentation tank at 28°C, and add sterilized L-phenylalanine. Inoculate 1% active Saccharomyces cerevisiae by flame burning at the inoculation port of the fermenter, and at the same time add 0.5 g / L of propyl gallate at a speed of 350r / min for fermentation culture. After 4 hours, the speed was adjusted to 600r / min, the ventilation rate of sterile air was 1.0 V / V·min, and the temperature was kept unchanged at 28°C. When both glucose and ethanol are consumed completely, the biotransformation is ende...

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Abstract

The invention discloses a method for preparing 2-phenylethyl alcohol, which comprises the following steps: adding active saccharomyces cerevisiae in a biotransformation medium, taking L-phenylalanine as a substrate and glucose as a carbon source, adding an anti-oxidant, and fermenting under aerobic condition to obtain 2-phenylethyl alcohol. According to the invention, L-phenylalanine is taken as the substrate, glucose is taken as carbon source, under an alternative oxidation degraded system of glucose and ethanol, the anti-oxidant with certain concentration is added, and perfume 2-phenylethyl alcohol can be obtained through batch fermentation. The method increases the synthesis rate of products 2-phenylethyl alcohol, shortens biotransformation period; employs a method for disposable addition of the anti-oxidant to reduce the content of impurities such as butanols, isobutyric acid, butyric acid and isovaleric acid during the biotransformation process, and increases the 2-phenylethyl alcohol fragrance quality.

Description

technical field [0001] The invention belongs to the field of chemical industry, in particular to a kind of 2-phenylethanol, specifically a method for preparing 2-phenylethanol. Background technique [0002] 2-phenylethanol (2-phenylethanol, 2-PE) is an aromatic alcohol with a fresh, elegant and delicate rose smell. Natural 2-PE exists in many plants in nature, especially rose essential oil. Because of its delicate and comfortable aroma, it has become the second largest spice after vanillin, and can be used in the deployment of cosmetics, tobacco flavors and various food flavors. At present, the global annual output of 2-PE is nearly 10,000 tons, which is basically produced by chemical synthesis. Only a very small part is extracted from rose essential oil, and its price is very different from that of synthetic 2-PE. The price of -PE is about 150 times, and the chemically synthesized 2-PE uses benzene or styrene as raw materials. These raw materials are highly carcinogenic, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/22C12R1/865
CPCC12P7/22
Inventor 荣绍丰田勋李茜茜蔡保国管世敏
Owner SHANGHAI INST OF TECH
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