137results about How to "Less amount of reagent" patented technology

The invention provides a microfluidic chip integrating circulating tumor cell capture, lysis and nucleic acid detection, and a device and method of microfluidic chip. The microfluidic chip is formed by laminating a glass base layer and a polydimethylsiloxane chip layer. The polydimethylsiloxane chip layer comprises the following parts: a circulating tumor cell capture zone; a nucleic acid detection zone comprising a feeding channel with a flexible sidewall, a microcavity array region and a sample outlet; and a valve control region comprising: T-shaped pipe structures respectively disposed on both sides of the feeding channel, wherein the structure includes a first pipe extending parallel to the feeding channel and a second pipe vertically connected to the feeding channel, and the second pipe is connected to a gas supply mechanism to control the opening and closing of the feeding channel by controlling the opening and closing of the gas supply mechanism. According to the present invention, the microfluidic chip integrating the circulating tumor cell capture, lysis and nucleic acid detection, and the device and method have the advantages of high throughput, low cost, high sensitivity, easy usage, and short time consumption.

The invention discloses hepatopathy detecting test paper by colloidal gold chromatography and a preparation method thereof. The hepatopathy detecting test paper by colloidal gold chromatography is a film tape formed by sequentially and mutually overlapping and pasting a pyroxylin coating film, a bonding pad, a sample pad and a water-absorption pad, wherein the pyroxylin film is provided with a detecting line and a quality control line, the detecting line coats M2 type mitochondrial antigenic protein, the quality control line coats an antibody (c), and the bonding pad coats a colloidal gold labeled antibody (a) and a colloidal gold labeled antibody (b). In the test paper tape provided by the invention, the M2 type mitochondrial antigenic protein is introduced, and process optimization is carried out on the bonding pad and the sample pad, thus realizing detection performance with high sensitivity, high specificity and high accuracy on the M2 type anti-mitochondrial antibody.

The invention relates to a method for detecting antioxidant constituents in nitraria fruit juice by using a DPPH-HPLC method. The method comprises that firstly an antioxidant 1,1-diphenyl-2-trinitrobenzene hydrazine and the nitraria fruit juice are subjected to photophobic reaction to obtain nitraria fruit juice after reaction; then nitraria fruit juice which is not subjected to reaction and the nitraria fruit juice after reaction are respectively injected into the high performance liquid chromatography for detection, and a chromatogram of the nitraria fruit juice which is not subjected to reaction and a chromatogram of the nitraria fruit juice after reaction are respectively ontained; and finally, peak areas of corresponding peaks in the chromatogram of the nitraria fruit juice which is not subjected to reaction and the chromatogram of the nitraria fruit juice after reaction are compared; and if the peak areas of the peaks are reduced, antioxidant constituents exist in the nitraria fruit juice. According to the method, the amount of the used reagent is low, the operation is convenient and simple, the sensitivity is high, the accuracy is high, the stability is good, and effective evaluation for the antioxidant constituents in nitraria fruit juice can be achieved.

The invention relates to the class of chemical technologies, belongs to the field of solid waste recovery and particularly relates to a method for recovering high-purity metal palladium from an organic waste palladium contained catalyst. The method comprises the steps of waste pretreatment, leaching, extraction, purification and hydrogenation preparation, thereby finally obtaining high-purity metal palladium. The method provided by the invention is simple in reaction step, short in time and capable of saving reagents; in addition, the recovery rate of palladium is up to over 99.2%, and the purity of recovered palladium is higher than 99.98%.

The present invention discloses a preparation method of an electrogenerated chemiluminescence sensor with a quantum dot modified nano porous carbon paste electrode for detecting trace antibiotic residues. The preparation method for the carbon paste electrode comprises the following steps: synthesizing nanoporous gold and quantum dot solution according to the existing method; modifying light-emitting quantum dots into the surface and the porous channels of the nanoporous gold material layer by layer by adopting the layer-by-layer self-assembly modification technology; doping the modified nanoporous gold material into graphite powder. The method for detecting the trace antibiotic residues comprises the following steps: connecting the modified carbon paste electrode with an electrogenerated chemiluminescence instrument, and detecting the trace antibiotic residues in dairy and meat products. The sensor has high sensitivity and a high detection speed, and one basic detection process can be completed within only 2-5 minutes. Moreover, the sensor has a low cost, and is economical and practical. The instrument is easy to operate and can automatically record and analyze experimental results, the effect of subjective factors is avoided, and good repeatability is achieved.

The invention relates to a method for detecting an antioxidant ingredient in Lycium ruthenicum Murr fruit juice with a DPPH-HPLC method. The method comprises the steps of reacting an antioxidant 1,1-dibenzyl-2-trinitrophenylhydrazine and the Lycium ruthenicum Murr fruit juice in a dark place to obtained reacted Lycium ruthenicum Murr fruit juice; respectively injecting unreacted Lycium ruthenicum Murr fruit juice and the reacted Lycium ruthenicum Murr fruit juice in a high performance liquid chromatography (HLPC) for detection to obtain a chromatogram of the unreacted Lycium ruthenicum Murr fruit juice and a chromatogram of the reacted Lycium ruthenicum Murr fruit juice; and finally comparing peak areas of corresponding peaks in the chromatogram of the unreacted Lycium ruthenicum Murr fruit juice and the chromatogram of the reacted Lycium ruthenicum Murr fruit juice, and if the peal areas of the reacted Lycium ruthenicum Murr fruit juice are smaller than those of and the unreacted Lycium ruthenicum Murr fruit juice, the result indicates that the Lycium ruthenicum Murr fruit juice contains the antioxidant ingredient. The method has the characteristics of little reagent, simple and convenient operation, high sensitivity, high precision, good stability and the like, and can realize effectiveness evaluation for the antioxidant ingredient in the Lycium ruthenicum Murr fruit juice.

The invention discloses a solid electrochemical luminescence sensor for detecting mercury ions and a preparation method and application of the solid electrochemical luminescence sensor for detecting the mercury ions. The solid electrochemical luminescence sensor is characterized by being a glassy carbon electrode, wherein the surface of the glassy carbon electrode is immobilized with electrically polymerized ABEI, and then the glassy carbon electrode is assembled with glutaraldehyde, DNA1, T-Hg<2+>-T mismatched DNA2 and biotin. The preparation method comprises the steps: preparing the glassy carbon electrode immobilized with the DNA1 and assembling the solid electrochemical luminescence sensor. The solid electrochemical luminescence sensor has the advantages of high sensitivity, high stability, high selectivity, high repetitiveness, easiness in operation and the like, and reagents can be saved.

The invention relates to a bioinstrumentation detection and identification method, in particular to a method for PCR detection of a pathogen of the citrus greening disease. The method for PCR detection of the pathogen of the citrus greening disease comprises the steps that DNA of a sample to be detected is extracted; a PCR amplification system is prepared, wherein the PCR amplification system comprises a primary PCR amplification system and a secondary PCR amplification system; a PCR amplification reaction is conducted; PCR amplification products are detected, wherein the final volume of the primary PCR amplification system and the final volume of the secondary PCR amplification system respectively ranges from 7microliters 10 microliters, wherein the final volume of a DNA template is 2.5 microliters, the final volume of 2*Taq PCR Master Mix is 2.5 microliters, the final volume of a primer is 0.25 microliter, and the rest is sterilization double distilled water. According to the method for PCR detection of the pathogen of the citrus greening disease, the final volume of the primary PCR amplification system and the final volume of the secondary PCR amplification system respectively ranges from 7microliters 10 microliters, the detection effect of a 20-microliter reaction system or a 25-microliter reaction system can be achieved, the economic cost of detection is greatly reduced, the amount of needed reagents is small, and the pollution to the environment is reduced.

The invention relates to a pretreatment method for detecting organochlorine pesticide residue. The method comprises the steps of (1) pulping fresh vegetables and fruits to obtain a sample to be detected; (2) adding acetonitrile into the sample to be detected for blending, then adding dried NaCl, centrifuging, and standing at room temperature to obtain supernate, evaporating the supernate to obtain a concentrate; (3) adding leacheate into the concentrate, and completely dissolving to obtain a solution A; (4) prewashing an amino column or a graphitized carbon black-amino serial column by the leacheate, removing the leacheate, and pouring the solution A instantly; (5) conducting column chromatography on the solution A, then adding the leacheate, and collecting to obtain a solution B; (6) evaporating the elution solution B until the constant weight is achieved to obtain eluate, conducting constant volume on the eluate by normal hexane; and (7) filtering the eluate, adding the eluate into an automatic sample feeding bottle, and the detecting the eluate by a gas chromatography electron capture detector (ECD). According to the method, the simplicity and the rapidness are realized, the pesticide residues in the vegetables and the fruits are extracted, and the quantitative detection of the pesticide residues can be realized.

The invention relates to a quality control method of lycium ruthenicum pigment. In the method, determination of a total anthocyanin content, a proanthocyanidins content and a total polyphenols content is taken as a quality control index, and the method has the advantages of little dosage of used reagent, simple and convenient operation, high flexibility, high precision, favorable stability and the like and can realize effective evaluation of biological activity on a lycium ruthenicum pigment.

The invention discloses a method for rapid determination of the selenium content in tetrastigmatis hemsleyani, wherein a microwave digestion-hydride generation-atomic fluorescence spectrometry method is adopted for determination of the selenium content. With microwave digestion, the digestion efficiency is high and fast, moreover, acid removing is not required after digestion, operation steps are simplified, pollution is reduced, the accuracy rate is increased, disadvantages of easy pollution of wet digestion and long digestion time are avoided, acid removing is not required, concentrated hydrochloric acid is directly added for reduction of selenium, and the digestion procedures are simplified; the detection cycle is greatly shortened, and the original detection cycle of 8-10 hours is shortened to 2-3 hours; and the detection is rapid and accurate, and the operation is simple.

The invention discloses a pretreatment kit for detecting Tilmicosin in an animal muscle tissue and a method thereof. The kit comprises a box body, a reagent tube A, a reagent bag B, a reagent tube C, a reagent bottle D, a reagent bottle E, and a reagent bag F, wherein the inside of the box body is divided into a zone a, a zone b, a zone c and a zone d, the zone a and the zone b are aperture zonesfor placing the reagent tube A and the reagent tube C, the zone c is a groove zone for placing the reagent bag B and the reagent bag F, and the zone d is a groove zone for placing the reagent bottle D and the reagent bottle E. The method comprises the following steps: 1, placing the animal muscle tissue in a centrifuge tube, adding a reagent to the centrifuge tube, and oscillating; 2, adding a reagent, and carrying out rotary evaporation to dryness; 3, adding a reagent to an inverted conical bottle, and transferring the obtained solution to the centrifuge tube; 4, adding a reagent solution, oscillating, and drying through blowing by a pressured gas blowing concentrator; and 5, dissolving the residue by a mixed liquid reagent solution, adding n-hexane, carrying out vortex oscillation, removing the upper liquid, filtering the lower liquid by a filtering head, and determining by UPLC (ultrahigh performance liquid chromatography). The method of the invention has the advantages of simple operation, rapidness, low price, and realization of no damage of the reagents to the chromatographic column.

The invention discloses a method for detecting NO (nitric oxide) content in exhaled gas of a human body, wherein PTIO (3-oxo-2-phenyl-4,4,5,5-tetramethyl imidazoline-1-oxygen) is utilized as an oxidizing agent of the NO, and the content of the NO in the exhaled gas of the human body can be directly and quickly detected by adopting an electrospray ionization extraction mass spectrometry method. The method disclosed by the invention has strong specificity and high sensitivity without pre-treatment on a sample; the PTIO has better stability and reaction sensitivity, and can selectively react with the NO. In addition, the method is integrated with sample collection and treatment, so that the consumed dose is small, the operation is convenient and simple and the detection can be finished in a short time. The method has the advantages of high mass spectrum sensitivity and good stability, breaks through the disadvantage of a traditional method, and realizes fast and sensitive monitoring of the NO in the exhaled gas of the human body.

The invention provides a fast visual detection method of organophosphorus type pesticide. Firstly, ZnTPyP is subjected to hydrothermal self-assembly by a cationic surface active agent for preparing nanometer porphyrin; then, the nanometer porphyrin is used for the fast visual detection on organophosphorus. On the basis of biomimetic peroxidase type activity of the nanometer porphyrin, 3,3',5,5'-tetramethyl benzidine is catalyzed and oxidized; the characteristic absorption in a 652nm position of the ultraviolet-visible spectrum occurs; colorless to blue chromogenic reaction is generated; specific reaction of the organophosphorus type pesticide and the nanometer porphyrin is utilized; the catalytic activity of the nanometer porphyrin is reduced; the generation of blue oxidation product oxTMBis reduced; the reduction of the absorbancy in the 652nm position of the ultraviolet-visible spectrum is shown; the reduction degree and the concentration of the organophosphorus type pesticide are in positive correlation relationship; the detection of the organophosphorus type pesticide is realized; the operation of the detection method is simple and convenient; the reagent toxicity is small; the cost is low; the sensitivity is high; the stability is high; the applicability is high.

The invention provides a fluorescent quantitative (PCR) rapid diagnostic kit for quantitatively detecting virus content of spleen and lymph tissue origin and cell culture origin classical swine fever virus C-strain vaccine, and a preparation method thereof. The method can achieve specific amplification when a total RNA content of the virus is 10<2> copies/[mu]L, has a good amplification curve in a linear range of 10<7>-10<2> copies/[mu]L, is suitable for titration evaluation of finished products and half-finished products of the lapinized classical swine fever virus vaccine. The specific primers of the classical swine fever virus (CSFV) used by the invention is two primers designed and synthesized in highly conserved 5' untranslated region (5'-UTR), wherein P1(5'-GCAGAAGCCCACCTCGAGAT-3') is a specific upstream primer of the classical swine fever virus; P2(5'-TACACCGGTTCCTCCACTCC-3') is a specific downstream primer of the classical swine fever virus; an expected amplification product is 245 bp; fluorescent quantitative (PCR) reaction presents a characteristic S-type amplification dynamic curve; and analysis of a melting curve shows a single characteristic peak. The method can only detect CSFV, and has no cross-reaction with BVDV.

The invention discloses a colloidal gold chromatography anti-SSB antibody detection test paper and a preparation method thereof. The colloidal gold chromatography anti-SSB antibody detection test paper comprises a sample pad, a combination pad, a cellulose nitrate coated film and a water absorption pad, and the sample pad, the combination pad, the cellulose nitrate coated film and the water absorption pad are pasted on a base plate sequentially from one side of the base plate to the other side of the base plate, wherein the combination pad is coated with a gold-labeled antibody a and a gold-labeled antibody b; and the cellulose nitrate coated film is provided with a detection line and a quality control line, the detection line is coated with an SSB antigen protein, and the quality control line is coated with a gold-labeled antibody c. By adopting indirect immunoassay to introduce the SSB antigen protein in the invention to carry out process optimization on the combination pad and the sample pad, so high sensitivity, high specificity and high accuracy detection performances of the anti-SSB antibody are realized, and a reference basis is provided for the auxiliary diagnosis of primary biliary cirrhosis.

The invention provides a quick antibacterial test method and a kit. The kit comprises an aseptic porous detection board, a moisturizing and heat preservation culture box, a culture solution, test organisms and a positive control antibacterial agent, wherein the aseptic porous detection board comprises three or more detection holes with the same size, transparent materials with the same thicknesses and uniform texture are arranged at the bottoms of the detection holes, the moisturizing and heat preservation culture box is composed of a box body and a box cover, 1-10 detection boards can be placed in the box body, the culture solution is a culture solution necessary for the growth of the test organisms. The quick antibacterial test method and the kit provided by the invention can be used for quickly and conveniently detecting the antibacterial ability of a test sample and have the advantages of high throughput and low cost.

The invention discloses a colloidal gold chromatography anti-Ro52 antibody detection test paper and a preparation method thereof. The colloidal gold chromatography anti-Ro52 antibody detection test paper comprises a sample pad, a combination pad, a cellulose nitrate coated film and a water absorption pad, and the sample pad, the combination pad, the cellulose nitrate coated film and the water absorption pad are pasted on a base plate sequentially from one side of the base plate to the other side of the base plate, wherein the combination pad is coated with a gold-labeled antibody a and a gold-labeled antibody b; and the cellulose nitrate coated film is provided with a detection line and a quality control line, the detection line is coated with an Ro52 antigen protein, and the quality control line is coated with a gold-labeled antibody c. By adopting indirect immunoassay to introduce the Ro52 antigen protein in the invention to carry out process optimization on the combination pad and the sample pad, so high sensitivity, high specificity and high accuracy detection performances of the anti-Ro52 antibody are realized, and a reference basis is provided for the auxiliary diagnosis of Sjogren's syndrome.

The invention discloses a micro-fluidic chip, a chemiluminescent immunoassay system and an analysis method. The micro-fluidic chip comprises: a base plate, on which a first liquid inlet channel, a second liquid inlet channel, a third liquid inlet channel, an immunoreaction pool and a luminescent reaction pool are formed. The discharge end of the immunoreaction pool is communicated with the liquidinlet end of the luminescent reaction pool, and the immunoreaction pool is internally equipped with a primer. The drain end of the first liquid inlet channel is communicated with the liquid inlet endof the immunoreaction pool, and the first liquid inlet channel is used for guiding a to-be-detected antigen and an antibody into the immunoreaction pool. The drain end of the second liquid inlet channel is communicated with the liquid inlet end of the immunoreaction pool, and the second liquid inlet channel is used for guiding an alkaline solution into the immunoreaction pool. The drain end of thethird liquid inlet channel is communicated with the liquid inlet end of the luminescent reaction pool, and the third liquid inlet channel is used for guiding an oxidant solution into the luminescentreaction pool. The micro-fluidic chip provided by the invention can realize chemiluminescence immunoassay with low reagent dose.