Application of enzyme labeling meter in latex enhancing immune transmittance turbidimetry

A technology of turbidimetry and latex immunity, which is applied in instruments, scientific instruments, measuring devices, etc., can solve the problems of high detection cost and large amount of reagents, and achieve the effects of short time consumption, small amount of reagents, and low cost

Inactive Publication Date: 2006-06-14
陈金华
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AI Technical Summary

Problems solved by technology

The biochemical analyzer is easy to operate and has high accuracy, but each test requires a large amount of reagents and high testing costs

Method used

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Embodiment Construction

[0013] The invention provides a detection method using a microplate reader for latex-enhanced immunotransmission turbidimetry. All immunotransmission turbidity through latex-enhanced turbidity can be detected by a microplate reader. Take transferrin in human serum as an example to illustrate , the specific implementation is as follows:

[0014] 1. The polystyrene latex was prepared according to the known technology (published by the People's Health Publishing House for rapid inspection data compilation, edited by the inspection team of Shanghai Sixth People's Hospital, 1973, pages 1-40), and 0.1mol / L pH8.2 glycine buffer was taken. 1ml of 2% polystyrene latex (latex particle size: 0.1um) diluted with liquid, add 100μl rabbit anti-transferrin antibody, add glycine buffer to dilute to 5ml, centrifuge at 10,000g for 10 minutes, aspirate the supernatant, add glycine buffer Diluted, washed twice in this way, and finally diluted to 1 ml with glycine buffer to obtain rabbit anti-tran...

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Abstract

The invention offers immunity transmission turbidimetry used enzyme mark instrument to detect insolubility immunity grain compound enhanced turbidity by latex extinction value. The method includes the following steps: combining antibody with polystyrene latex to form antibody latex; combining the antibody latex with albumen antigen of blood serum sample; when immune response happens they will do chain reaction that a lot of latex is gathered to form insolubility immunity grain compound; the grain size of the latex changes and its transmission capacity and absorbance value also changes. The invention adopts enzyme mark instrument with certain transmitting wavelength to measure the change of the latex grain absorbance value, and draws reaction standard curve of the antigen and corresponding antibody latex grain to calculate identity antigen density in sample.

Description

Technical field: [0001] The invention relates to an immunotransmission turbidimetric method, in particular to a detection method with a microplate reader. technical background: [0002] The immunoturbidimetric method is a turbidimetric method that uses the antigenic properties of the complex structure of the protein to form an insoluble immune complex after the protein to be tested is combined with a specific antibody, and is irradiated with visible light to detect the protein content. However, the turbidity change of this immune complex is too small, and the absorbance value changes after visible light irradiation is small, and the sensitivity is very low. After research and improvement, the latex-enhanced immune turbidimetric method was produced. The latex-enhanced immune turbidimetric method is to bind antibody molecules to the surface of latex. The commonly used latex is polymer microspheres with a particle size of 0.1um to 0.2um. When the particle size of the latex is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/546G01N21/31
Inventor 陈金华
Owner 陈金华
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