156 results about "Stylosanthes viscosa" patented technology

The invention provides a beta 2-microglobulin detection kit comprising a reagent R1 and a reagent R2, wherein the reagent R1 comprises a buffer solution, a preservative, a stabilizing agent, an electrolyte, a surfactant and the balance of purified water; and the reagent R2 comprises polystyrene latex grains sensitized by the beta 2-microglobulin antibody, a buffer solution, a preservative, a stabilizing agent and a residual amount of purified water. The polystyrene latex grains sensitized by the beta 2-microglobulin antibody are directionally coupled. A preparation method comprises the following steps of: activating the polystyrene latex grains; oxidizing the beta 2-microglobulin antibody; and coupling the beta 2-microglobulin antibody and the polystyrene latex grains. The kit provided by the invention has the advantages of high sensitivity, good specificity, good accuracy, strong anti-jamming capability and low production cost.

The invention discloses a hollow ball micro/nano structure array with a plurality of silver nano plates as basic elements and a preparation method thereof. In the array, a hollow gold ball array is arranged on a substrate, fan-shaped silver nano plates are arranged on gold ball shells in a crossing and standing way, wherein the inside diameter of the gold ball shells is 1-10mu m, the thickness of the gold ball shells is 15-25nm, the height of the fan-shaped silver nano plates is 500mn to 1mu m, the width of the fan-shaped silver nano plates is 200-500nm, the thickness of the fan-shaped silver nano plates is 20-30nm, and the gold ball shells are made of single silver crystal. In the method, polystyrene colloidal balls with the diameter being 1-10mu m are attached to the surface of the substrate at first to form a single-layer colloidal crystal template, a gold film is evaporated on the surface of the single-layer colloidal crystal template, the single-layer colloidal crystal template with the evaporated gold film on the surface is placed in silver electrolyte for electrodeposition, intermediate products are obtained after cleaning and drying and are then placed in dichloromethane solution to dissolve polystyrene colloidal balls, and thus the hollow ball micro/nano structure array with the silver nano plates as the basic elements is prepared. The hollow ball micro/nano structure array can be used as an SERS (surface enhanced Raman scattering) active substrate for rapid trace detection of a variety of organic pollutants.

The invention discloses a two-dimensional and double-cycle ordered structure array and a preparation method thereof. The array is an ordered porous film consisting of metals in a microparticle hole shape and a nanometer hole shape and arranged on a conductive substrate, the nanometer hole in the film is located in the microparticle hole and is in a stacking shape, or the nanometer hole is a single layer and is located at the bottom surface or the external surface of the microparticle hole or covers the internal surface and the external surface thereof, the diameter of the microparticle hole is 1,800-2,200 nm, and the diameter of the thenanometer hole is 180-220 nm. The method comprises the following steps of: firstly, self-assembling by using a polystyrene colloidal ball of one diameter in combination with a solution impregnating method or an electrodepositing method to obtain the ordered hole array of a bowl-shaped metal attached to the conductive substrate; and then self-assembling thereon by utilizing polystyrene colloidal of another diameter in combination with the electrodepositing method to prepare the two-dimensional and double-cycle ordered structure arrays in four structures. The product thereof has the characteristics of a macro-scale system, the preparation method has universality, and the two-dimensional double-cycle and ordered structure array consisting of other conductive materials can be prepared by the method.

The invention discloses a detection kit for human serum amyloid A protein. The detection kit is characterized by comprising a reagent R1 and a reagent R2, the reagent R1 is an appropriate buffer solution, and the reagent R2 is prepared by putting polystyrene latex particles sensitized by a human serum amyloid A protein antibody into the appropriate buffer solution. The detection kit has the advantages of being simple and fast in detection, high in sensitivity, good in accuracy, high in anti-jamming capability and low in production cost.

The invention discloses an anti-cyclic citrullinated peptide (CCP) antibody detection kit. The kit comprises a reagent R1, a reagent R2, a standard substance and a standard diluent, wherein the reagent R1 is prepared by washing CCP antigen latex particles by 50mM Tris buffer solution containing 20-500mmol/L first stabilizer and 0.1-1% preservative and then dispersing; and CCP antigens and polystyrene latex microspheres are subjected to chemical crosslinking and then are closed, so that the CCP antigen latex particles are formed. For the kit, the latex-enhanced immunoturbidimetry is adopted for detecting the content of an anti-CCP antibody, the kit has the high sensitivity, the high stability and the high detection speed (the time from determination to result acquisition is only within 5-10min), can detect mass samples on a conventional biochemical analyzer and can greatly improve the detection efficiency.

The invention relates to a preparation method of cystatin C antibody and a preparation method of a detection kit. Cystatin C (Cys C) is currently one of the most sensitive diagnostic markers for clinical kidney disease. The antibody preparation of the present invention is extracted and purified from human normal serum, and then a highly sensitive antigen is obtained by artificial modification. Animals were immunized multiple times to obtain hyperimmune serum containing Cys C antibody, and high-purity Cys C antibody was obtained by affinity chromatography. During the preparation process, the loss of antibody activity is small, and it is easy to produce in batches, and the antibody has high affinity and high titer, and does not generate cross-immune reaction. After the antibody is coupled with polystyrene latex balls, the prepared detection kit has higher sensitivity and linear range, good repeatability, low detection limit, easy automatic analysis, fast and simple operation. Due to the high purity of the antibody, the content of heteroantibody is very small, so there is less interference and the matrix is ​​stable.

The invention discloses a detection kit for a cystine protease inhibitor C. The kit comprises a reagent R1 and a reagent R2, wherein the R1 is a proper buffer, and the reagent R2 is formed through placing polystyrene latex particles sensitized by cystine protease inhibitor C antibodies in a proper buffer. The kit of the present invention has the advantages of high sensitivity, good specificity, good accuracy, strong anti-interference capability, and low production cost.

The invention discloses a reagent kit for measuring the content of C-reactive protein in whole blood and a measuring method thereof. The reagent kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 comprises a surfactant and a proper amount of buffer solution, and the content of the surfactant is 0.1-3g/L; and the reagent 2 comprises polystyrene latex adsorbed with a C-reactive protein antibody and a proper amount of buffer solution, and the content of the polystyrene latex adsorbed with the C-reactive protein antibody is 0.5-3g/L. The measuring method comprises the following steps of: collecting a whole blood sample of a subject, uniformly mixing the reagent 1 and the whole blood sample, then adding the reagent 2 for uniformly mixing, detecting the absorbance and the scattering light intensity of a reaction system at 625-800nm, and computing the content of the C-reactive protein in the whole blood by contrasting a standard curve. In the reagent kit and the measuring method, the whole blood of the subject can be used as a measured sample, the operation is simple, and results are accurate. The invention relieves the pain of the subjects, particularly for the child and newborn subjects and the subjects with more difficult vein blood collection, such as patients with large-area burns, and the like.

The invention provides a latex enhanced turbidimetric immunoassay kit for quantitatively detecting cystatin C. The kit comprises a reagent R1 and a reagent R2 which are independent from each other, wherein the reagent R1 is mainly prepared from a buffer solution 1, a stabilizing agent 1, a preservative 1, EDTA, accelerator and a protective agent 1; the reagent R2 is mainly prepared from polystyrene latex microspheres for crosslinking a cystatin C antibody, a buffer solution 2, a stabilizing 2, a preservative 2 and a protective agent 2, wherein the polystyrene latex microspheres and the cystatin C antibody are connected in a covalent cross-linking mode. The detection kit has the advantages of low preparation cost, good stability, good data repeatability and high detection sensitivity, is easy to store, and can be widely applied to clinical biochemical analyzers.

The invention discloses a silicon nano cone array coated with a gold film as well as a preparation method and an application thereof. The array is a silicon nano cone sequence array with a surface coated with a gold film, wherein the conical bottom diameter of silicon nano cones forming the silicon nano cone sequence array is 180nm to 220nm, the cone height is 450nm to 550nm, the cone period is 250nm to 350nm, and the thickness of the gold film is 15nm to 25nm. The preparation method comprises the following steps: first synthesizing polystyrene colloidal spheres on a silicon substrate to form a single-layer colloidal crystal template by virtue of a gas-liquid interface self-assembling technology, placing the silicon substrate with the single-layer colloidal crystal template in a sulfur hexafluoride atmosphere, etching the silicon substrate by virtue of plasma to obtain the silicon substrate with silicon nano cone sequence array, and depositing the gold film on the silicon substrate with the silicon nano cone sequence array by utilizing an ion beam sputtering technology or thermal evaporation deposition technology to obtain a target product. The silicon nano cone array can be used as an active substrate of surface enhanced raman scattering to measure the content of clenbuterol hydrochloride attached thereon.

The invention provides a method for detecting cardiac troponin I (cTnI) and a cardiac troponin I detection kit prepared by applying the method. The method comprises the steps of coupling an antibody composition with polystyrene latex particles, then subjecting the coupled material to immunoreaction with corresponding antigen in a sample to be detected to form aggregated particles, and determining turbidity generated by the reactant under a wavelength of 400-700nm to obtain the content of cTnI in the sample to be detected. The antibody composition consists of at least three antibodies, and the antibodies are specific to cTnI without cross reaction with fast skeletal muscle troponin I and slow skeletal muscle troponin I. In addition, if one antibody in the antibody composition is sensitive to a certain factor influencing the accurate determination of cTnI in the sample to be detected, and then at least another antibody is not sensitive to the factor influencing the accurate determination of cTnI. The method of the invention is simple to operate, and has a wide scope of application, high precision, strong capability of resisting disturbance and low production cost.

The invention relates to a perovskite catalyst with a three-dimensional ordered macroporous structure, in particular to preparation and application of the perovskite catalyst with the three-dimensional ordered macroporous structure. The preparing process comprises the following steps: (1), preparing polystyrene colloidal crystals by a colloidal crystal template process; (2), assembling the colloidal crystals by a centrifugal method; (3), filling a template with a precursor solution prepared from metal ion nitrate by an impregnating method; (4), removing the template by a high-temperature forging method; (5) applying the perovskite catalyst with the three-dimensional ordered macroporous structure to the fields of new energy, such as lithium air batteries, catalysis, environments, chemicals and electronic industry. The operation is simple, and the prepared perovskite catalyst with the three-dimensional ordered macroporous structure has great catalytic activity. When the perovskite catalyst with the three-dimensional ordered macroporous structure is applied to the lithium air batteries, the electrochemical properties of the batteries can be obviously improved, and high catalytic activity is reflected.

The invention provides a cystatin C detection kit and a preparation method thereof. The kit comprises a reagent R1, a reagent R2 and a cystatin C calibration product, wherein the reagent R1 comprisesa preservative, a surfactant, an emulsifier and a buffer solution; the reagent R2 comprises a cystatin C antibody, polystyrene latex microspheres, a latex microsphere activating agent, a sealing agent, an emulsifier, a preservative, a surfactant and a buffer solution. The cystatin C antibody is slowly dropwise added to an activated polystyrene latex microsphere solution, a polystyrene latex microsphere suspension coupled with the cystatin C antibody is obtained after a stirring reaction, sealing is performed, the antibody and latex microspheres are coupled together in a chemical crosslinking manner, and centrifugation, cleaning and ultrasonic resuspension processes in conventional methods are omitted. The production process is simplified, and the kit has the advantages of low blank absorbency, high sensitivity and precision and large linear range.

The invention discloses a latex enhanced immuno-nephelometry kit for determining procalcitonin and a preparation method and application of the latex enhanced immuno-nephelometry kit for determining procalcitonin, and belongs to the technical field of disease diagnosis and detection. The latex enhanced immuno-nephelometry kit for determining the procalcitonin comprises a diluent, a latex preparation, a blank liquid, a calibrator and a quality control material, wherein the latex preparation contains carboxylated polystyrene latex with different particle sizes and coupled with a PCT monoclonal antibody and a PCT polyclonal antibody respectively. According to the method, the PCT monoclonal antibody and the PCT polyclonal antibody are marked respectively, the latex with the large particle size can improve the detection sensitivity, and the latex with the small particle size can expand the linearity range, and therefore, the composite latex preparation can improve the detection sensitivity, can expand the detection linearity range, and has the advantages of high detection speed, high sensitivity, strong specificity and good accuracy for detection on the procalcitonin.

The invention relates to an in vitro diagnostic kit and in particular relates to a D-dimmer latex-reinforced immunonephelometry detection kit cooperatively adopting an ion stabilizer and a suspension stabilizer. The D-dimmer latex-reinforced immunonephelometry detection kit cooperatively adopting the ion stabilizer and the suspension stabilizer comprises two components, namely a reagent R1 and a reagent R2, wherein the reagent R1 mainly consists of a buffer solution 1, a stabilizer 1, a preservative 1, a coagulation accelerator and a protective agent 1; the reagent R2 mainly consists of a buffer solution 2, two polystyrene latex microspheres crosslinked with different D-dimmer monoclonal antibodies, a stabilizer 2, a protective agent 2 and a preservative 2, and the stabilizer 2 in the reagent R2 adopts the ion stabilizer and the suspension stabilizer which are used cooperatively. Compared with the prior art, the D-dimmer latex-reinforced immunonephelometry detection kit cooperatively adopting the ion stabilizer and the suspension stabilizer has the advantages that a latex-reinforced immunonephelometry method is adopted, and detection can be carried out under the condition that wavelength is 400-800nm, so that the detection is easier and the D-dimmer latex-reinforced immunonephelometry detection kit cooperatively adopting the ion stabilizer and the suspension stabilizer can be applied in clinical more easily.

The invention discloses a binary ordered colloidal crystal template and a preparation method and use thereof. The template consists of a substrate and colloidal crystals on the substrate, wherein particularly the colloidal crystals consist of polystyrene colloidal spheres with two different diameters in a ratio of (5-20):1; the diameter of the big-diameter colloidal spheres is 250 to 5,000 nanometers, and the diameter of the small-diameter colloidal spheres is 50 to 250 nanometers; the big-diameter colloidal spheres are arranged in a single layer according to a hexagonal ordered structure; the small-diameter colloidal spheres are positioned between the big-diameter colloidal spheres; and the colloidal crystals are 250 to 5,000 nanometers thick. The method comprises the following steps: uniformly mixing big-diameter polystyrene colloidal sphere solution and small-diameter polystyrene colloidal spheres solution, the mass percentage solubility of each of which is 1.5 to 10 percent, according to a volume ratio of (10-30):1 to obtain mixed solution; dripping the mixed solution onto a substrate; spinning and casting; and naturally drying to obtain the binary ordered colloidal crystal template. The binary ordered colloidal crystal template can be widely used for preparing a single-layer or multi-layer binary ordered porous thin film.

The invention provides a preparing method for a metal/oxide compound surface enhanced Raman active substrate. The method solves the problem that according to an existing technical scheme, a metal/oxide compound substrate with a multilayer film structure is hard to prepare, meanwhile, the preparing method can be suitable for compounding of a plurality of kinds of metal and oxides, and the application range is wide. According to the method, firstly, a self-assembly technology is used for preparing a two-dimensional ordered polystyrene colloidal array; then, a plasma etching technology is used for etching the two-dimensional ordered polystyrene colloidal array; thirdly, the polystyrene colloidal array subject to treatment in the second step serves as a substrate, and under the protection atmosphere or vacuum conditions, a metal/oxide compound multi-layer film sequentially grows on the surface of the substrate through a magnetron sputtering method; and finally, heat treatment is carried out under the protection atmosphere, and the surface enhanced Raman activeness of the metal/oxide compound surface enhanced Raman active substrate obtained after special heat treatment is obviously enhanced.

The invention discloses a preparation method of a silicon substrate array with nanometer gaps being controllable and application thereof. The preparation method comprises the steps that a silicon slice substrate single-layer polystyrene colloidal crystal array is prepared; etching is conducted on the silicon slice substrate single-layer polystyrene colloidal crystal array by adopting a reactive ion etching method, the single-layer polystyrene colloidal crystal array is removed after etching is completed, and a tapered silicon substrate array is prepared; and the tapered silicon substrate arrayis taken as a template, a layer of gold film with the thickness being 10-50nm is deposited on the surface of the template by adopting a physical deposition method, gold nanoballs are deposited and formed at the top of the tapered silicon substrate, the distances among the gold nanoballs are adjusted by controlling the deposition time, and the silicon substrate array with the nanometer gaps beingcontrollable is prepared by controlling the deposition time and adjusting the distances among the gold nanoballs. The silicon substrate array is large in structure area, clean on surface, high in sensitivity and good in detection performance, can directly serve as a substrate material which has long-term stability and a high-activity surface enhanced Raman effect, and can be used for conducting rapid trace detection on the concentration of saccharin sodium salt. The preparation method is simple, convenient to operate, low in cost and economical and environmentally friendly.

The invention provides a cystatin C latex-particle-enhanced turbidimetry detection reagent kit and application thereof.The reagent kit comprises a reagent R1 and a reagent R2.The reagent R1 is prepared from a buffer solution, inorganic salt, a surfactant, a preservative, a stabilizer and interference elimination protein.The reagent R2 is prepared from a buffer solution, inorganic salt, a surfactant, a preservative, a stabilizer and a polystyrene latex particle mixture, and the polystyrene latex particle mixture is interlinked with a cystatin C antibody.The reagent kit based on latex-particle-enhanced turbidimetric immunoassay (PETIA) can be generally applied to analysis of various full-automatic biochemical analyzers and is short in assay time, good in specificity, high in precision and good in accuracy when used.

The invention relates to the making of 3DOM. It is made through synthesizing polymethyl methacryate or polystyrene pattern lumber, using Li and Mn front drive solution to stuff glue crystalline, through two sections of constant temperature baking to get 3DOM, using acid or peroxy-disulfate to perform acid etch. The result is that the product is the low density material in small blocks, unnecessary to add adhesive with high adhesive feature, using ammonium peroxy-disulfate to remove Li, reduced in Mn ion damage, improved adhesive feature, showing three dimensional porous frame structure, significantly improved in drive power inside the screen and the outside surface of the micro hole adhesive, being a dual hole channel with both macro and micro holes.

The invention discloses a preparation method for polystyrene latex with carboxyl on the surface and for immunoassay technology; the preparation method comprises the following steps that: styrene, methyl methacrylate and water are added into a three-neck flask to be stirred for 10 to 30min; the molar ratio of the methyl methacrylate to the styrene is 0.1 to 0.2:1; after stirring, N2 is fed into the reaction system; and then potassium persulfate and the aqueous solution of sodium chloride are added in, so that the concentration of sodium chloride in the reaction system is 0.064mol/L, and the concentration of the potassium persulfate is 7.00*10-4 to 25.0*10-3mol/L; N2 is continued to be fed in; the temperature goes up to 60 to 80DEG C, and the reaction is carried out for 12 to 24h; and after the reaction, the temperature is cooled to room temperature, and the target product of the invention is prepared. The latex prepared by the invention has the advantages of uniform grain size, good monodispersity and strong stability; and simultaneously, the surface of the latex has higher carboxyl content, and the preparation method is applicable to a latex immune turbidity determination method.

The invention provides a lipoprotein phospholipase A2 detection kit which comprises reagent R1 and reagent R2, the reagent comprises a buffer solution, a stabilizing agent, electrolyte, a surface active agent and preservative; the reagent R2 comprises polystyrene latex particles coupled to an antibody resisting to the lipoprotein phospholipase A2, a buffer solution, a stabilizing agent, a surface active agent and preservative. A directional coupling method is adopted for the polystyrene latex particles sensitized by the antibody resisting to the lipoprotein phospholipase A2, and the preparation steps include activation of the polystyrene latex particles; oxidation of the antibody resisting to the lipoprotein phospholipase A2; coupling of the antibody resisting to the lipoprotein phospholipase A2 to the polystyrene latex particles. The kit is high in detection sensitivity, wide in detection range, good in accuracy and high in interference resistance.

The invention discloses a preparation method of a three-dimensional ordered macroporous carrier loaded heteropoly compound catalyst and belongs to the technical field of preparation of catalysts. The preparation method comprises the following steps of: adding tetraethoxysilane, a vanadium-containing compound and hydrochloric acid into a polystyrene colloidal crystal template; immersing and calcining the template to obtain a VOx-SiO2 three-dimensional ordered macroporous carrier; modifying the carrier; and finally, introducing a heteropoly compound to obtain the three-dimensional ordered macroporous carrier loaded heteropoly compound catalyst. According to the preparation method of the three-dimensional ordered macroporous carrier loaded heteropoly compound catalyst, disclosed by the invention, the catalyst has a proper duct structure and a relatively large specific surface area, so that the diffusing process of reactants and products is facilitated; and the oxidation-reduction quality of the catalyst can be improved by doping vanadium in the carrier, so that the catalyst shows high activity and high selectivity in catalyzing the oxidizing reaction of methylacrolein.

The invention relates to an in vitro diagnostic kit, and in particular relates to a D dimer latex-enhanced immunoturbidimetric assay kit utilizing a surface functional group. The D dimer latex-enhanced immunoturbidimetric assay kit utilizing the surface functional group consists of two components, namely a reagent R1 and a reagent R2, wherein the reagent R1 mainly consists of a buffer solution 1, a stabilizer 1, a preservative 1, a gelling agent and a protective agent 1; the reagent R2 mainly consists of a buffer solution 2, two polystyrene latex microspheres cross-linked to different D dimer monoclonal antibodies, a stabilizer 2, a protective agent 2 and a preservative 2; the polystyrene latex microspheres are connected to the D dimer antibodies in a manner of covalent cross-linking or physical adsorption; and the surface functional group of the polystyrene latex microsphere in the reagent R2 is an amino group, a carboxyl group, hydrazide, an aldehyde group or an epoxy group. Compared with the prior art, by virtue of the latex-enhanced immunoturbidimetric assay, the kit can be used for detecting by wavelength of 400-800nm, so that the kit is more convenient to detect and is easy in clinical application.